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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHH320
Library SH
(Link to library)
Clone ID SHH320
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15687-1
Original site URL
Representative seq. ID SHH320P
(Link to Original site)
Representative DNA sequence 
>SHH320 (SHH320Q) /CSM/SH/SHH3-A/SHH320Q.Seq.d/
GAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGGTCAAAATGAAGA
ATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGAGCAACAATTAAC
TAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGATAGAAGAAGCATT
AGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTCAATTTATAATAA
ACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCATAAAATTTTCAATAA
AGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTAAAAGAAACAGCAGA
AAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGAATCATCAGAAGG
TGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTTATCAGCATTTGT
AAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATCGATCTATCAATC
ACAAAAGAATATACATACACAATTCTTTAAGAATGTTCATTTACAAGCTGTCTATTATTC
AATGATTGATATATTTTCAGTATTAATAAATTTAGATAGTATAATTACACAAAATACTGC
TTTAGAXXXXXXXXXXGTAATGCTATCAAATTCGTACCAGACTTGAAAAAGATTCCAAAA
TTCCAAGATTTAACCTCAAAAGATGCCCTCTCACCTGAAACAATTCAAGCTAGTACCAAT
TTGGATTCAATCATTCATAATCTTTCAAATAATTTATCAGAGGGTACTGAATACTTTAAG
ATGTTGGTAAACGTTTTCGCTACAGAGTTTCGAAATATCGCTAATCAACATTTAAAGAAT
TTCTATATCATCGTACCTGCCTTGGCCTCAAATTACATCGATCATATGATCAATAGTAAA
GATAAACTCTTTAAGAAATCAAAAGCAATCGGTGCTGAAGCTTTATTATTCACTGATGAT
GGCTTTGCAATTGGTTTGGCTTATATCTTAAAATTATTAGATCAAAATAAAGATTTCGAT
TCTCTTCATTGGTTCGATCGTATTCAATTGAAATGTGAAACTGATCAAAAGAGAATGTTG
GCTGAAGCAAATGCAAAAGGTATCAAAGAGGATCAAAATCATTTAGCAATTAAAAAGATT
CAAAATTTCCAACAAGAATATG
sequence update 2002.10.25
Translated Amino Acid sequence 
nnpi*KMATSYSANNGQNEEYTFDAFEDGSSKLVGEQQLTKLNQFAVDYAQQIWKIEEAL
DETLNEVWDINIDPVSIYNKPHEQLDLIELVKTDHKIFNKVILVFSSICNQTRILKETAE
SKFYSPLTVFGEITGESSEGDVQIEVGKLLPFMIDLSAFVNRCYSLIRNIISQFASIYQS
QKNIHTQFFKNVHLQAVYYSMIDIFSVLINLDSIITQNTAL---

---NAIKFVPDLKKIPKFQDLTSKDALSPETIQASTNLDSIIHNLSNNLSEGTEYFKMLV
NVFATEFRNIANQHLKNFYIIVPALASNYIDHMINSKDKLFKKSKAIGAEALLFTDDGFA
IGLAYILKLLDQNKDFDSLHWFDRIQLKCETDQKRMLAEANAKGIKEDQNHLAIKKIQNF
QQEY
Translated Amino Acid sequence (All Frames) 
Frame A:
eqpnlenghilfg**wsk*riyi*ci*rwfi*igwratin*t*sics*lcttnledrrsi
r*nik*smgykyrssfnl**ts*tirfn*ig*n*s*nfq*snisilfnl*sn*nfkrnsr
k*ilftinsiwrdyr*iirr*ctn*sw*iitiyd*fisick*mlfin*kyy*sicidlsi
tkeytytil*ecsftscllfnd*yifsinkfr*ynytkycfr---

---vmlsnsyqt*krfqnski*pqkmpshlkqfklvpiwiqsfiifqiiyqrvlntlrcw
*tfslqsfeislini*risissylpwpqitsii*sivkinslrnqkqsvlklyyslmmal
qlvwlis*ny*ikikisilfigsivfn*nvklikrecwlkqmqkvskrikii*qlkrfki
snknm

Frame B:
nnpi*KMATSYSANNGQNEEYTFDAFEDGSSKLVGEQQLTKLNQFAVDYAQQIWKIEEAL
DETLNEVWDINIDPVSIYNKPHEQLDLIELVKTDHKIFNKVILVFSSICNQTRILKETAE
SKFYSPLTVFGEITGESSEGDVQIEVGKLLPFMIDLSAFVNRCYSLIRNIISQFASIYQS
QKNIHTQFFKNVHLQAVYYSMIDIFSVLINLDSIITQNTAL---

---*cyqirtrlekdskiprfnlkrcplt*nnss*yqfgfnhs*sfk*firgy*il*dvg
krfryrvskyr*stfkeflyhrtclglklhrsydq**r*tl*eiksnrc*sfiih**wlc
nwfglylkiirsk*rfrfsslvrsysiem*n*skenvg*skckryqrgsksfsn*kdskf
ptri

Frame C:
ttqfrkwphpirlimvkmknihlmhlkmvhlnwlesnn*lnlinlqlimhnkfgr*kkh*
mkh*mkygi*i*iqfqfiinlmnn*i*lnwlkliikfsik*y*yslqfviklef*kkqqk
vnfihh*qylerlqvnhqkvmyklklvnyyhl*liyqhl*idvih*leillvnlhrsinh
kriyihnslrmfiyklsiiq*liyfqy**i*iv*lhkill*---

---NAIKFVPDLKKIPKFQDLTSKDALSPETIQASTNLDSIIHNLSNNLSEGTEYFKMLV
NVFATEFRNIANQHLKNFYIIVPALASNYIDHMINSKDKLFKKSKAIGAEALLFTDDGFA
IGLAYILKLLDQNKDFDSLHWFDRIQLKCETDQKRMLAEANAKGIKEDQNHLAIKKIQNF
QQEY
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHH320 (SHH320Q) /CSM/SH/SHH3-A/SHH320Q.Seq.d/                       2363   0.0
VHQ112 (VHQ112Q) /CSM/VH/VHQ1-A/VHQ112Q.Seq.d/                       1320   0.0
VHP507 (VHP507Q) /CSM/VH/VHP5-A/VHP507Q.Seq.d/                       1320   0.0
VHP184 (VHP184Q) /CSM/VH/VHP1-D/VHP184Q.Seq.d/                       1320   0.0
VHI654 (VHI654Q) /CSM/VH/VHI6-C/VHI654Q.Seq.d/                       1320   0.0
VHH832 (VHH832Q) /CSM/VH/VHH8-B/VHH832Q.Seq.d/                       1320   0.0
VHA138 (VHA138Q) /CSM/VH/VHA1-B/VHA138Q.Seq.d/                       1320   0.0
SHL271 (SHL271Q) /CSM/SH/SHL2-C/SHL271Q.Seq.d/                       1320   0.0
CHO318 (CHO318Q) /CSM/CH/CHO3-A/CHO318Q.Seq.d/                       1320   0.0
CHI453 (CHI453Q) /CSM/CH/CHI4-C/CHI453Q.Seq.d/                       1320   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BY464171|BY464171.1 Mus musculus 15 days pregnant adult female amnion cDNA, RIKENsequence.    42   0.026  2
CP000123|CP000123.1 Mycoplasma capricolum subsp. capricolum ATCC 27343, complete genome.    38   0.034  20
CR450713|CR450713.2 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    38   0.046  8
AE017308|AE017308.1 Mycoplasma mobile 163K complete genome.        38   0.050  18
BB822673|BB822673.1 Mus musculus mammary gland RCB-0526 Jyg-MC(A) cDNA, RIKEN full-length enriched library, clone:G830022O04, 3' end partial sequence.    32   0.074  4
BX842643|BX842643.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 2/4.    34   0.082  13
CR103667|CR103667.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP142o16.    50   0.12   1
CR023465|CR023465.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP421e10.    50   0.12   1
CR044130|CR044130.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP186g09.    50   0.12   1
CR017539|CR017539.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP47e19.    50   0.12   1
dna update 2006. 4.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54R74) RecName: Full=UPF0681 protein;                               426   e-117
AB028956_1(AB028956|pid:none) Homo sapiens mRNA for KIAA1033 pro...   173   9e-42
BC143373_1(BC143373|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   173   9e-42
BC040936_1(BC040936|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   173   9e-42
BC020314_1(BC020314|pid:none) Mus musculus RIKEN cDNA A230046K03...   171   5e-41
(Q3UMB9) RecName: Full=UPF0681 protein KIAA1033;                      171   5e-41
AK161247_1(AK161247|pid:none) Mus musculus adult male testis cDN...   171   5e-41
AK145000_1(AK145000|pid:none) Mus musculus mammary gland RCB-052...   171   5e-41
BC110850_1(BC110850|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   167   9e-40
CR858798_1(CR858798|pid:none) Pongo abelii mRNA; cDNA DKFZp468B1...   153   1e-35
protein update 2009. 4.19
PSORT 

 psg: 0.79  gvh: 0.25  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 40.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for SHH320 is nuc
5' end seq. ID SHH320F
5' end seq. 
>SHH320F.Seq
GAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGGTCAAAATGAAGA
ATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGAGCAACAATTAAC
TAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGATAGAAGAAGCATT
AGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTCAATTTATAATAA
ACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCATAAAATTTTCAATAA
AGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTAAAAGAAACAGCAGA
AAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGAATCATCAGAAGG
TGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTTATCAGCATTTGT
AAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATCGATCTATCAATC
ACAAAAGAATATACATACACAATTCTTTAAGAATGTTCATTTACAAGCTGTCTATTATTC
AATGATTGATATATTTTCAGTATTAATAAATTTAGATAGTATAATTACACAAAATACTGC
TTTAGANNNNNNNNNN
Length of 5' end seq. 676
3' end seq. ID SHH320Z
3' end seq. 
>SHH320Z.Seq
NNNNNNNNNNGTAATGCTATCAAATTCGTACCAGACTTGAAAAAGATTCCAAAATTCCAA
GATTTAACCTCAAAAGATGCCCTCTCACCTGAAACAATTCAAGCTAGTACCAATTTGGAT
TCAATCATTCATAATCTTTCAAATAATTTATCAGAGGGTACTGAATACTTTAAGATGTTG
GTAAACGTTTTCGCTACAGAGTTTCGAAATATCGCTAATCAACATTTAAAGAATTTCTAT
ATCATCGTACCTGCCTTGGCCTCAAATTACATCGATCATATGATCAATAGTAAAGATAAA
CTCTTTAAGAAATCAAAAGCAATCGGTGCTGAAGCTTTATTATTCACTGATGATGGCTTT
GCAATTGGTTTGGCTTATATCTTAAAATTATTAGATCAAAATAAAGATTTCGATTCTCTT
CATTGGTTCGATCGTATTCAATTGAAATGTGAAACTGATCAAAAGAGAATGTTGGCTGAA
GCAAATGCAAAAGGTATCAAAGAGGATCAAAATCATTTAGCAATTAAAAAGATTCAAAAT
TTCCAACAAGAATATG
Length of 3' end seq. 556
Connected seq. ID SHH320P
Connected seq. 
>SHH320P.Seq
GAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGGTCAAAATGAAGA
ATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGAGCAACAATTAAC
TAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGATAGAAGAAGCATT
AGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTCAATTTATAATAA
ACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCATAAAATTTTCAATAA
AGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTAAAAGAAACAGCAGA
AAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGAATCATCAGAAGG
TGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTTATCAGCATTTGT
AAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATCGATCTATCAATC
ACAAAAGAATATACATACACAATTCTTTAAGAATGTTCATTTACAAGCTGTCTATTATTC
AATGATTGATATATTTTCAGTATTAATAAATTTAGATAGTATAATTACACAAAATACTGC
TTTAGA----------GTAATGCTATCAAATTCGTACCAGACTTGAAAAAGATTCCAAAA
TTCCAAGATTTAACCTCAAAAGATGCCCTCTCACCTGAAACAATTCAAGCTAGTACCAAT
TTGGATTCAATCATTCATAATCTTTCAAATAATTTATCAGAGGGTACTGAATACTTTAAG
ATGTTGGTAAACGTTTTCGCTACAGAGTTTCGAAATATCGCTAATCAACATTTAAAGAAT
TTCTATATCATCGTACCTGCCTTGGCCTCAAATTACATCGATCATATGATCAATAGTAAA
GATAAACTCTTTAAGAAATCAAAAGCAATCGGTGCTGAAGCTTTATTATTCACTGATGAT
GGCTTTGCAATTGGTTTGGCTTATATCTTAAAATTATTAGATCAAAATAAAGATTTCGAT
TCTCTTCATTGGTTCGATCGTATTCAATTGAAATGTGAAACTGATCAAAAGAGAATGTTG
GCTGAAGCAAATGCAAAAGGTATCAAAGAGGATCAAAATCATTTAGCAATTAAAAAGATT
CAAAATTTCCAACAAGAATATG
Length of connected seq. 1212
Full length Seq ID -
Full length Seq. -
Length of full length seq. -