>SHH309 (SHH309Q) /CSM/SH/SHH3-A/SHH309Q.Seq.d/
ATTCCTGCTCACCATCAACTGGTTGCGTAAATACCCCAATTTCTTGTGATGACAAAAACC
CATGCACCGTAGACTCTTGCAACAATTCAACTGGTTGCTGTTATACCCCAATCAATGTTG
ATGACAATAATCCATGTACCATCGATGCCTGTACTAAATCAACAGGTGTCACCCACACTC
CAATCAATGTCGATGATAATAACCAATGTACAACTGATTCATGTACCAAAGAAGGTGGTG
TAACTCATACTCCAGTCAATACTGATGATAACAATCCATGTACTGTTGACTCCTGCTCAC
CATTTACAGGTGTTTCTCACACTCCAATCAATGTTGATGATAATAACANATGTACAATTG
ATGCATGTACCAAAGAANGTGGTGTAACTCATACTCCANTCAACACTGATGATAACAATG
CCTGTACCCTTGATTCTTGTTCACCATAAACTGGTGTGACTCATACACCCAATAAANTGT
GANGATAAAAAAAGCCTGTACNNGTGGATTCATGTTCACAANTCAACTGGNGTGCGTANA
TACTCCAATNTANTGTGGATGATAATAATCCATGTNCNGTTGATACCTGTGANGACTCNA
CCGGGGTGTTGNAATACTCCCATCAAATGTTGATGATAANNAANACATGTGCCNCTNGAT
GCNCTGTGCCAAAAAAAXXXXXXXXXXCCAAAAGATAAATGTTCAATCTCTCAATGTGAT
TCAGCCAAAGGTTGCATCGAAGTCCCAATGAATTGTACCTCTGATAAATGTAATGAAGCA
TCATGTTGTGATGGTGTTTGTACCTCAAAACCAATTAGCTGTCCAAAACCAAAGAATAAG
TGTCAAGTTGCAAAATGTGATTTAATTAAAGGTTGTACCGTCTCAAATGTAGTATGTGAT
GATGGTAATGCTTGTACCGAAGATTCATGTTGTTCAGACACTGGTAAATGTCAATTCGAA
CCAATCAAACTTCCAAAGAATAAAAACAAATGTATCATTTCAAAATGTGATCCAATTAAA
GGTACAATCACCAACAGTACCGTAAACTGTGAATGTGATGACCTTTGTAACATTGGTGAA
TGTTGTGAAGATACAGGAAAATGTAATTACAGACAAAAAGATTGTGATGATAATAATCCA
AAAACAGCTGATAGTTGCGATTCCAAGACTGGTAAATGTATTAACAAACCATATAATGTT
ATCACAAGTGGTTCTAATTTAATCTCTGGTTTAATTGGTGGTCTCATTGGTGGTGGTACA
GGAGGTAAAGGNATGCAAAACTGTAAAAATTAAATAACTCTTTT

scspstgcvntpiscddknpctvdscnnstgccytpinvddnnpctidactkstgvthtp
invddnnqcttdsctkeggvthtpvntddnnpctvdscspftgvshtpinvddnnxctid
actkexgvthtpxntddnnactldscsp*tgvthtpnkx*x*KKPVXVDSCSQXNWXAXI
LQXXVDDNNPCXVDTCXDSTGVLXYSHQMLMIXXTCAXXCXVPKK---

---PKDKCSISQCDSAKGCIEVPMNCTSDKCNEASCCDGVCTSKPISCPKPKNKCQVAKC
DLIKGCTVSNVVCDDGNACTEDSCCSDTGKCQFEPIKLPKNKNKCIISKCDPIKGTITNS
TVNCECDDLCNIGECCEDTGKCNYRQKDCDDNNPKTADSCDSKTGKCINKPYNVITSGSN
LISGLIGGLIGGGTGGKGMQNCKN*itl

Frame A:
ipahhqlva*ipqflvmtkthap*tlatiqlvavipqsmlmtiihvpsmpvlnqqvsptl
qsmsmiitnvqlihvpkkvv*lilqsilmitihvlltpahhlqvfltlqsmlmiitxvql
mhvpkxvv*lilxstlmitmpvplilvhhklv*lihpixcxdkkslyxwihvhxstgvrx
ysnxxwmiiihvxlipvxtxpgcxntpikc***xxhvpldalcqk---

---PKDKCSISQCDSAKGCIEVPMNCTSDKCNEASCCDGVCTSKPISCPKPKNKCQVAKC
DLIKGCTVSNVVCDDGNACTEDSCCSDTGKCQFEPIKLPKNKNKCIISKCDPIKGTITNS
TVNCECDDLCNIGECCEDTGKCNYRQKDCDDNNPKTADSCDSKTGKCINKPYNVITSGSN
LISGLIGGLIGGGTGGKGMQNCKN*itl

Frame B:
flltinwlrkypnfl**qkpmhrrllqqfnwlllypnqc**q*smyhrcly*inrchphs
nqcr***pmyn*fmyqrrwcnsyssqy***qsmyc*llltiyrcfshsnqc****xmyn*
cmyqrxwcnsysxqh***qclyp*flftinwcdsytq*xvxikkactxgfmftxqlxcvx
tpxxcg***smxx*yl*xlxrgvxilpsnvddxxxmcxxmxcakk---

---qkinvqslnviqpkvasksq*ivplinvmkhhvvmvfvpqnqlavqnqrisvklqnv
i*lkvvpsqm*yvmmvmlvpkihvvqtlvnvnsnqsnfqriktnvsfqnviqlkvqsptv
p*tvnvmtfvtlvnvvkiqenvitdkkivmiiiqkqlivaiprlvnvltnhimlsqvvli
*slv*lvvslvvvqevkxcktvkik*lf

Frame C:
scspstgcvntpiscddknpctvdscnnstgccytpinvddnnpctidactkstgvthtp
invddnnqcttdsctkeggvthtpvntddnnpctvdscspftgvshtpinvddnnxctid
actkexgvthtpxntddnnactldscsp*tgvthtpnkx*x*KKPVXVDSCSQXNWXAXI
LQXXVDDNNPCXVDTCXDSTGVLXYSHQMLMIXXTCAXXCXVPKK---

---kr*mfnlsm*fsqrlhrspnelyl**m**siml*wclylktn*lsktke*vssckm*
fn*rlyrlkcsm**w*clyrrfmlfrhw*msirtnqtske*kqmyhfkm*sn*rynhqqy
rkl*m**pl*hw*ml*ryrkm*lqtkrl****skns**lrfqdw*my*qti*cyhkwf*f
nlwfnwwshwwwyrr*rxakl*klnnsf

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHH309 (SHH309Q) /CSM/SH/SHH3-A/SHH309Q.Seq.d/                       1217   0.0
CFC542 (CFC542Q) /CSM/CF/CFC5-B/CFC542Q.Seq.d/                       1189   0.0
SHA152 (SHA152Q) /CSM/SH/SHA1-C/SHA152Q.Seq.d/                       1187   0.0
CFC807 (CFC807Q) /CSM/CF/CFC8-A/CFC807Q.Seq.d/                       1181   0.0
SHF556 (SHF556Q) /CSM/SH/SHF5-C/SHF556Q.Seq.d/                       1179   0.0
SHG408 (SHG408Q) /CSM/SH/SHG4-A/SHG408Q.Seq.d/                       1178   0.0
SHC394 (SHC394Q) /CSM/SH/SHC3-D/SHC394Q.Seq.d/                       1178   0.0
SFC263 (SFC263Q) /CSM/SF/SFC2-C/SFC263Q.Seq.d/                       1178   0.0
CFC677 (CFC677Q) /CSM/CF/CFC6-D/CFC677Q.Seq.d/                       1178   0.0
SHG420 (SHG420Q) /CSM/SH/SHG4-A/SHG420Q.Seq.d/                       1176   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X78948|X78948.1 D.minutum ecmB gene.                               50   2e-07  4
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    50   0.13   1
AF467890|AF467890.1 Mus musculus chromosome 17 clone 160i3 strain C57BL/6J, *** SEQUENCING IN PROGRESS ***, 4 ordered pieces.    48   0.52   1
AC154238|AC154238.2 Mus musculus chromosome 17 clone RP24-162O23, WORKING DRAFT SEQUENCE.    48   0.52   1
DX470312|DX470312.1 Cpl12344 Papaya Genomic DNA BAC Library Carica papaya genomic, genomic survey sequence.    48   0.52   1
AC138642|AC138642.10 Mus musculus chromosome 3, clone RP23-21O7, complete sequence.    48   0.52   1
AC122491|AC122491.3 Mus musculus BAC clone RP24-390D3 from chromosome 3, complete sequence.    48   0.52   1
AC113114|AC113114.9 Mus musculus chromosome 17, clone RP23-338H10, complete sequence.    48   0.52   1
AC117072|AC117072.3 Dictyostelium discoideum chromosome 2 map 3879572-4071762 strain AX4, complete sequence.    42   2.0    4
BB223484|BB223484.1 Mus musculus adult male aorta and vein cDNA, RIKEN full-length enriched library, clone:A530082F12, 3' end partial sequence.    46   2.0    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P11976) RecName: Full=Prestalk protein; AltName: Full=Extracell...   276   e-101
A26838(A26838)prestalk protein precursor - slime mold (Dictyoste...   276   e-101
A27020(A27020)DIF-induced prestalk pDd63 protein precursor - sli...   341   4e-92
S44208(S44208) extracellular matrix protein B - Dictyostelium mi...   224   4e-57
AC116984_103(AC116984|pid:none) Dictyostelium discoideum chromos...   211   6e-53
AC117072_61(AC117072|pid:none) Dictyostelium discoideum chromoso...   193   8e-51
(Q54C31) RecName: Full=Protein psiR; Flags: Precursor;                 92   5e-29
(Q54C32) RecName: Full=Protein psiQ; Flags: Precursor;                 87   6e-26
(Q86HT1) RecName: Full=Protein psiL; Flags: Precursor;  &AC11695...    68   1e-19
(Q54CH8) RecName: Full=Protein psiN; Flags: Precursor;                 98   5e-19

 psg: 0.82  gvh: 0.54  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.09  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 36.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for SHH309 is nuc

>SHH309F.Seq
ATTCCTGCTCACCATCAACTGGTTGCGTAAATACCCCAATTTCTTGTGATGACAAAAACC
CATGCACCGTAGACTCTTGCAACAATTCAACTGGTTGCTGTTATACCCCAATCAATGTTG
ATGACAATAATCCATGTACCATCGATGCCTGTACTAAATCAACAGGTGTCACCCACACTC
CAATCAATGTCGATGATAATAACCAATGTACAACTGATTCATGTACCAAAGAAGGTGGTG
TAACTCATACTCCAGTCAATACTGATGATAACAATCCATGTACTGTTGACTCCTGCTCAC
CATTTACAGGTGTTTCTCACACTCCAATCAATGTTGATGATAATAACANATGTACAATTG
ATGCATGTACCAAAGAANGTGGTGTAACTCATACTCCANTCAACACTGATGATAACAATG
CCTGTACCCTTGATTCTTGTTCACCATAAACTGGTGTGACTCATACACCCAATAAANTGT
GANGATAAAAAAAGCCTGTACNNGTGGATTCATGTTCACAANTCAACTGGNGTGCGTANA
TACTCCAATNTANTGTGGATGATAATAATCCATGTNCNGTTGATACCTGTGANGACTCNA
CCGGGGTGTTGNAATACTCCCATCAAATGTTGATGATAANNAANACATGTGCCNCTNGAT
GCNCTGTGCCAAAAAAANNNNNNNNNN

>SHH309Z.Seq
NNNNNNNNNNCCAAAAGATAAATGTTCAATCTCTCAATGTGATTCAGCCAAAGGTTGCAT
CGAAGTCCCAATGAATTGTACCTCTGATAAATGTAATGAAGCATCATGTTGTGATGGTGT
TTGTACCTCAAAACCAATTAGCTGTCCAAAACCAAAGAATAAGTGTCAAGTTGCAAAATG
TGATTTAATTAAAGGTTGTACCGTCTCAAATGTAGTATGTGATGATGGTAATGCTTGTAC
CGAAGATTCATGTTGTTCAGACACTGGTAAATGTCAATTCGAACCAATCAAACTTCCAAA
GAATAAAAACAAATGTATCATTTCAAAATGTGATCCAATTAAAGGTACAATCACCAACAG
TACCGTAAACTGTGAATGTGATGACCTTTGTAACATTGGTGAATGTTGTGAAGATACAGG
AAAATGTAATTACAGACAAAAAGATTGTGATGATAATAATCCAAAAACAGCTGATAGTTG
CGATTCCAAGACTGGTAAATGTATTAACAAACCATATAATGTTATCACAAGTGGTTCTAA
TTTAATCTCTGGTTTAATTGGTGGTCTCATTGGTGGTGGTACAGGAGGTAAAGGNATGCA
AAACTGTAAAAATTAAATAACTCTTTT

>SHH309P.Seq
ATTCCTGCTCACCATCAACTGGTTGCGTAAATACCCCAATTTCTTGTGATGACAAAAACC
CATGCACCGTAGACTCTTGCAACAATTCAACTGGTTGCTGTTATACCCCAATCAATGTTG
ATGACAATAATCCATGTACCATCGATGCCTGTACTAAATCAACAGGTGTCACCCACACTC
CAATCAATGTCGATGATAATAACCAATGTACAACTGATTCATGTACCAAAGAAGGTGGTG
TAACTCATACTCCAGTCAATACTGATGATAACAATCCATGTACTGTTGACTCCTGCTCAC
CATTTACAGGTGTTTCTCACACTCCAATCAATGTTGATGATAATAACANATGTACAATTG
ATGCATGTACCAAAGAANGTGGTGTAACTCATACTCCANTCAACACTGATGATAACAATG
CCTGTACCCTTGATTCTTGTTCACCATAAACTGGTGTGACTCATACACCCAATAAANTGT
GANGATAAAAAAAGCCTGTACNNGTGGATTCATGTTCACAANTCAACTGGNGTGCGTANA
TACTCCAATNTANTGTGGATGATAATAATCCATGTNCNGTTGATACCTGTGANGACTCNA
CCGGGGTGTTGNAATACTCCCATCAAATGTTGATGATAANNAANACATGTGCCNCTNGAT
GCNCTGTGCCAAAAAAA----------CCAAAAGATAAATGTTCAATCTCTCAATGTGAT
TCAGCCAAAGGTTGCATCGAAGTCCCAATGAATTGTACCTCTGATAAATGTAATGAAGCA
TCATGTTGTGATGGTGTTTGTACCTCAAAACCAATTAGCTGTCCAAAACCAAAGAATAAG
TGTCAAGTTGCAAAATGTGATTTAATTAAAGGTTGTACCGTCTCAAATGTAGTATGTGAT
GATGGTAATGCTTGTACCGAAGATTCATGTTGTTCAGACACTGGTAAATGTCAATTCGAA
CCAATCAAACTTCCAAAGAATAAAAACAAATGTATCATTTCAAAATGTGATCCAATTAAA
GGTACAATCACCAACAGTACCGTAAACTGTGAATGTGATGACCTTTGTAACATTGGTGAA
TGTTGTGAAGATACAGGAAAATGTAATTACAGACAAAAAGATTGTGATGATAATAATCCA
AAAACAGCTGATAGTTGCGATTCCAAGACTGGTAAATGTATTAACAAACCATATAATGTT
ATCACAAGTGGTTCTAATTTAATCTCTGGTTTAATTGGTGGTCTCATTGGTGGTGGTACA
GGAGGTAAAGGNATGCAAAACTGTAAAAATTAAATAACTCTTTT