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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHH224
Library SH
(Link to library)
Clone ID SHH224
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11458-1
Original site URL
Representative seq. ID SHH224P
(Link to Original site)
Representative DNA sequence 
>SHH224 (SHH224Q) /CSM/SH/SHH2-A/SHH224Q.Seq.d/
TCAATCTACGTAATAAGTTATCCTATTTGTTAGGATTTTCCGCATGCTTCTTGGTAGTGT
TTATAGTGTCACTATGTGTTTCCATCATCACCAACACACCAGTGGTATTCCTAAGTTTGT
CAGAAGCAAGTGTTGGTGAAATGGATATGGTGTTGACACCTGGTTCATGGACATTATCAG
ATCAATTAAATTATACAATGTTATCAGAGTTATTAGCTGGTACATATAAAGAACCAAATG
GAAGTTATTTTGATGATAATAATAATAATAATCAATATGAATTAGAAGAAGTAGATGATA
GTTACCATTCACCAAGAGTCGATGGGACTGTTAGTGTGGTTAGTGCAAAGTCGTGTCCAA
ACGGTAACACAAATCCAAATGATAATCTTTGGAAATATCATGATGAGCCAAATCAATGTG
AACCATACTGTTTCGAATCAGTTTGTAGTAATTCAGTAGCAAGTAAAGCATCGATGTTTG
TGATTGATACTGCACGTGAAAAGAGGATGGGATTAGGTCGTGATTGGAAATTGGATGCTC
CACCACAGGGXXXXXXXXXXTCGTATGCAAGTAGTATCTTCTCATCAGTACGTGAAATTC
CAATGAATCAAGTGATTTTGAAATTGAATACTGATGATAAAGATGCTGTAGAATCAATAA
AGAATCGTTTAAATAGCTTTTTAATTGGTAATCTTAGAGAGGATTTCGATATGTTGGATT
ACTATGAAAAGATTAAACCTTTTGCAACGGCCTCTGTGATCATTCAATACTTTTTCAGTT
TTACTACAGTTTTAGCAATGACCATCTCTTTCTTCTCTNTAATGTCTTCTGTATATTCAA
ATATTTTAGAACAAACTAAAGAAATTGGTGTTTTATGTGCATTGGGAATACCAAAATCTT
GGATGATTAGAATTTACGTCTATGAAGCTTTCGTATTGGTTATGGCTTCCTCAATGTTGG
GTATGTTTATTGGTTCAACTGTTGGTTTCACAATGATCCTTCAAAGACAATTATTTACAA
TGTTACCAATTCCAATGACTTTACCTTGGCAATTAATTATAATAGTTTTCATTTGTTCAA
TGTTTTTCTCTTTCTTTTCAGCTTTTGGACCAATTAGAAAAGTACTTAATCAACCCGTTG
TTAGTATAAGAGAGTTGTAACTTAAAAAAATAAAATAAATAAATAAATAAAAAAATAATA
A
sequence update 2002.10.25
Translated Amino Acid sequence 
NLRNKLSYLLGFSACFLVVFIVSLCVSIITNTPVVFLSLSEASVGEMDMVLTPGSWTLSD
QLNYTMLSELLAGTYKEPNGSYFDDNNNNNQYELEEVDDSYHSPRVDGTVSVVSAKSCPN
GNTNPNDNLWKYHDEPNQCEPYCFESVCSNSVASKASMFVIDTAREKRMGLGRDWKLDAP
PQ---

---SYASSIFSSVREIPMNQVILKLNTDDKDAVESIKNRLNSFLIGNLREDFDMLDYYEK
IKPFATASVIIQYFFSFTTVLAMTISFFSXMSSVYSNILEQTKEIGVLCALGIPKSWMIR
IYVYEAFVLVMASSMLGMFIGSTVGFTMILQRQLFTMLPIPMTLPWQLIIIVFICSMFFS
FFSAFGPIRKVLNQPVVSIREL*lkkik*ink*knn
Translated Amino Acid sequence (All Frames) 
Frame A:
siyvisypic*dfphasw*cl*chyvfpsspthqwys*vcqkqvlvkwiwc*hlvhghyq
in*iiqcyqsy*lvhiknqmevilmiiiiiinmn*kk*mivtihqesmgllvwlvqsrvq
tvtqiqmiifgnimmsqinvnhtvsnqfvviq*qvkhrcl*lilhvkrgwd*vvignwml
hhr---

---SYASSIFSSVREIPMNQVILKLNTDDKDAVESIKNRLNSFLIGNLREDFDMLDYYEK
IKPFATASVIIQYFFSFTTVLAMTISFFSXMSSVYSNILEQTKEIGVLCALGIPKSWMIR
IYVYEAFVLVMASSMLGMFIGSTVGFTMILQRQLFTMLPIPMTLPWQLIIIVFICSMFFS
FFSAFGPIRKVLNQPVVSIREL*lkkik*ink*knn

Frame B:
qst**vilfvrifrmllgsvysvtmcfhhhqhtsgipkfvrskcw*ngygvdtwfmdiir
siklynvirviswyi*rtkwklf*******si*irrsr**lpftksrwdc*cg*ckvvsk
r*hksk**sleis**aksm*tilfrisl**fssk*sidvcd*yct*kedgirs*leigcs
ttg---

---rmqvvsshqyvkfq*ik*f*n*ilmikml*nq*riv*iaf*lvilerisicwitmkr
lnllqrpl*sfntfsvllqf*q*pslssl*cllyiqif*nklkklvfyvhweyqnlg*le
ftsmklsywlwlpqcwvcllvqllvsq*sfkdnylqcyqfq*lylgn*l**fsfvqcfsl
sfqlldqlekylinpllv*escnlkk*nk*inkkii

Frame C:
NLRNKLSYLLGFSACFLVVFIVSLCVSIITNTPVVFLSLSEASVGEMDMVLTPGSWTLSD
QLNYTMLSELLAGTYKEPNGSYFDDNNNNNQYELEEVDDSYHSPRVDGTVSVVSAKSCPN
GNTNPNDNLWKYHDEPNQCEPYCFESVCSNSVASKASMFVIDTAREKRMGLGRDWKLDAP
PQ---

---vck*yllist*nsnessdfeiey***rccrinkesfk*lfnw*s*rgfryvgll*kd
*tfcnglcdhsilfqfyysfsndhlfllxnvfcifkyfrtn*rnwcfmcigntkildd*n
lrl*sfrigygflnvgyvywfncwfhndpsktiiynvtnsndftlainynsfhlfnvflf
lfsfwtn*kst*strc*ykrvvt*knkink*ikk**
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHH224 (SHH224Q) /CSM/SH/SHH2-A/SHH224Q.Seq.d/                       1699   0.0
VHQ341 (VHQ341Q) /CSM/VH/VHQ3-B/VHQ341Q.Seq.d/                       1174   0.0
SHF215 (SHF215Q) /CSM/SH/SHF2-A/SHF215Q.Seq.d/                       1174   0.0
AHL653 (AHL653Q) /CSM/AH/AHL6-C/AHL653Q.Seq.d/                       1174   0.0
CHS122 (CHS122Q) /CSM/CH/CHS1-A/CHS122Q.Seq.d/                       1130   0.0
AHP863 (AHP863Q) /CSM/AH/AHP8-C/AHP863Q.Seq.d/                       1104   0.0
SSG440 (SSG440Q) /CSM/SS/SSG4-B/SSG440Q.Seq.d/                       1100   0.0
AHN177 (AHN177Q) /CSM/AH/AHN1-D/AHN177Q.Seq.d/                       1086   0.0
CHP271 (CHP271Q) /CSM/CH/CHP2-C/CHP271Q.Seq.d/                        509   e-143
VFL665 (VFL665Q) /CSM/VF/VFL6-C/VFL665Q.Seq.d/                         42   0.019
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AU088323|AU088323.2 Plasmodium falciparum 3D7 cDNA clone from Sugano malaria cDNA library: XPFn6930.    48   0.48   1
AU088296|AU088296.1 Plasmodium falciparum 3D7 cDNA clone from Sugano malaria cDNA library: XPFn6884.    48   0.48   1
AU087831|AU087831.1 Plasmodium falciparum 3D7 cDNA clone from Sugano malaria cDNA library: XPFn5944.    48   0.48   1
AU087192|AU087192.1 Plasmodium falciparum 3D7 cDNA clone from Sugano malaria cDNA library: XPFn3984.    48   0.48   1
AU087161|AU087161.1 Plasmodium falciparum 3D7 cDNA clone from Sugano malaria cDNA library: XPFn3933.    48   0.48   1
BQ633262|BQ633262.1 PfESToab39g07.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to TR:Q25991 Q25991 PARASITOPHOROUS VACUOLE ANTIGEN ;, mRNA sequence.    48   0.48   1
A16141|A16141.1 SERP gene.                                         48   0.48   1
AR567083|AR567083.1 Sequence 119 from patent US 6780407.           48   0.48   1
AR052783|AR052783.1 Sequence 119 from patent US 5833975.           48   0.48   1
AR288096|AR288096.1 Sequence 119 from patent US 6537594.           48   0.48   1
dna update 2006. 4. 9
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116956_66(AC116956|pid:none) Dictyostelium discoideum chromoso...    87   1e-15
CT005262_48(CT005262|pid:none) Leishmania major strain Friedlin,...    74   1e-11
AM502241_48(AM502241|pid:none) Leishmania infantum chromosome 23.      73   2e-11
CP001390_2000(CP001390|pid:none) Geobacter sp. FRC-32, complete ...    63   2e-08
CP000361_689(CP000361|pid:none) Arcobacter butzleri RM4018, comp...    62   5e-08
CP001213_713(CP001213|pid:none) Bifidobacterium animalis subsp. ...    61   9e-08
CP000937_337(CP000937|pid:none) Francisella philomiragia subsp. ...    61   9e-08
AP008971_1053(AP008971|pid:none) Finegoldia magna ATCC 29328 DNA...    60   2e-07
AM233362_474(AM233362|pid:none) Francisella tularensis subsp. ho...    60   2e-07
AY967184_1(AY967184|pid:none) Synthetic construct isolate FTT040...    60   2e-07
protein update 2009. 4.19
PSORT 

 psg: 0.99  gvh: 0.63  alm: 0.16  top: 0.13  tms: 0.27  mit: 0.40  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 36.0 %: endoplasmic reticulum
	 24.0 %: mitochondrial
	 12.0 %: plasma membrane
	  8.0 %: extracellular, including cell wall
	  8.0 %: cytoplasmic
	  4.0 %: vacuolar
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for SHH224 is end
5' end seq. ID SHH224F
5' end seq. 
>SHH224F.Seq
TCAATCTACGTAATAAGTTATCCTATTTGTTAGGATTTTCCGCATGCTTCTTGGTAGTGT
TTATAGTGTCACTATGTGTTTCCATCATCACCAACACACCAGTGGTATTCCTAAGTTTGT
CAGAAGCAAGTGTTGGTGAAATGGATATGGTGTTGACACCTGGTTCATGGACATTATCAG
ATCAATTAAATTATACAATGTTATCAGAGTTATTAGCTGGTACATATAAAGAACCAAATG
GAAGTTATTTTGATGATAATAATAATAATAATCAATATGAATTAGAAGAAGTAGATGATA
GTTACCATTCACCAAGAGTCGATGGGACTGTTAGTGTGGTTAGTGCAAAGTCGTGTCCAA
ACGGTAACACAAATCCAAATGATAATCTTTGGAAATATCATGATGAGCCAAATCAATGTG
AACCATACTGTTTCGAATCAGTTTGTAGTAATTCAGTAGCAAGTAAAGCATCGATGTTTG
TGATTGATACTGCACGTGAAAAGAGGATGGGATTAGGTCGTGATTGGAAATTGGATGCTC
CACCACAGGGNNNNNNNNNN
Length of 5' end seq. 560
3' end seq. ID SHH224Z
3' end seq. 
>SHH224Z.Seq
NNNNNNNNNNTCGTATGCAAGTAGTATCTTCTCATCAGTACGTGAAATTCCAATGAATCA
AGTGATTTTGAAATTGAATACTGATGATAAAGATGCTGTAGAATCAATAAAGAATCGTTT
AAATAGCTTTTTAATTGGTAATCTTAGAGAGGATTTCGATATGTTGGATTACTATGAAAA
GATTAAACCTTTTGCAACGGCCTCTGTGATCATTCAATACTTTTTCAGTTTTACTACAGT
TTTAGCAATGACCATCTCTTTCTTCTCTNTAATGTCTTCTGTATATTCAAATATTTTAGA
ACAAACTAAAGAAATTGGTGTTTTATGTGCATTGGGAATACCAAAATCTTGGATGATTAG
AATTTACGTCTATGAAGCTTTCGTATTGGTTATGGCTTCCTCAATGTTGGGTATGTTTAT
TGGTTCAACTGTTGGTTTCACAATGATCCTTCAAAGACAATTATTTACAATGTTACCAAT
TCCAATGACTTTACCTTGGCAATTAATTATAATAGTTTTCATTTGTTCAATGTTTTTCTC
TTTCTTTTCAGCTTTTGGACCAATTAGAAAAGTACTTAATCAACCCGTTGTTAGTATAAG
AGAGTTGTAACTTAAAAAAATAAAATAAATAAATAAATAAAAAAATAATAA
Length of 3' end seq. 651
Connected seq. ID SHH224P
Connected seq. 
>SHH224P.Seq
TCAATCTACGTAATAAGTTATCCTATTTGTTAGGATTTTCCGCATGCTTCTTGGTAGTGT
TTATAGTGTCACTATGTGTTTCCATCATCACCAACACACCAGTGGTATTCCTAAGTTTGT
CAGAAGCAAGTGTTGGTGAAATGGATATGGTGTTGACACCTGGTTCATGGACATTATCAG
ATCAATTAAATTATACAATGTTATCAGAGTTATTAGCTGGTACATATAAAGAACCAAATG
GAAGTTATTTTGATGATAATAATAATAATAATCAATATGAATTAGAAGAAGTAGATGATA
GTTACCATTCACCAAGAGTCGATGGGACTGTTAGTGTGGTTAGTGCAAAGTCGTGTCCAA
ACGGTAACACAAATCCAAATGATAATCTTTGGAAATATCATGATGAGCCAAATCAATGTG
AACCATACTGTTTCGAATCAGTTTGTAGTAATTCAGTAGCAAGTAAAGCATCGATGTTTG
TGATTGATACTGCACGTGAAAAGAGGATGGGATTAGGTCGTGATTGGAAATTGGATGCTC
CACCACAGGG----------TCGTATGCAAGTAGTATCTTCTCATCAGTACGTGAAATTC
CAATGAATCAAGTGATTTTGAAATTGAATACTGATGATAAAGATGCTGTAGAATCAATAA
AGAATCGTTTAAATAGCTTTTTAATTGGTAATCTTAGAGAGGATTTCGATATGTTGGATT
ACTATGAAAAGATTAAACCTTTTGCAACGGCCTCTGTGATCATTCAATACTTTTTCAGTT
TTACTACAGTTTTAGCAATGACCATCTCTTTCTTCTCTNTAATGTCTTCTGTATATTCAA
ATATTTTAGAACAAACTAAAGAAATTGGTGTTTTATGTGCATTGGGAATACCAAAATCTT
GGATGATTAGAATTTACGTCTATGAAGCTTTCGTATTGGTTATGGCTTCCTCAATGTTGG
GTATGTTTATTGGTTCAACTGTTGGTTTCACAATGATCCTTCAAAGACAATTATTTACAA
TGTTACCAATTCCAATGACTTTACCTTGGCAATTAATTATAATAGTTTTCATTTGTTCAA
TGTTTTTCTCTTTCTTTTCAGCTTTTGGACCAATTAGAAAAGTACTTAATCAACCCGTTG
TTAGTATAAGAGAGTTGTAACTTAAAAAAATAAAATAAATAAATAAATAAAAAAATAATA
A
Length of connected seq. 1191
Full length Seq ID -
Full length Seq. -
Length of full length seq. -