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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFJ471
Library AF
(Link to library)
Clone ID AFJ471
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10768-1
Original site URL
Representative seq. ID AFJ471P
(Link to Original site)
Representative DNA sequence 
>AFJ471 (AFJ471Q) /CSM/AF/AFJ4-C/AFJ471Q.Seq.d/
CACTGTTGGCCTACTGGNATTTGAATACATAATTTATATTTATTATTTTATAAATATATA
TTTTTAATATGGCACATATTGATCGTATTAATATACTTGCCAATCATCTTTCAAAGGAAG
AAGAAGAAGAAAATGAATTATTAACTTCACCAGTTTCTGCTGATAAAAAAAAAGAGACAG
TTACAGTTACAGATAATAGAAATGGTAAAAGCTATGATTTCAAAATTAAAAATGACACTA
TTAATGCTTTAAACTTTAAAGAATTACAATTAGCCAAAGGTGATGGTGGTTTAATGATTT
ATGATCCAGGTTTCCAAAATACAGCAGTTGTAACATCATACATTACATATATTGATGGTG
ATAAAGGAATTTTAAGATACAGAGGTTATCCAATTGAAGAATTAGCAGAAAGATCAAACT
TTTTAGAGGTTGCTTATCTTTTAATCAATGGTAACTTACCAAATAAATCACAATTAGATG
GTTGGAGTAATAAAATTATGACTCATACTTTTCTTCATGAAAATTTGGTTGGTCTTATGA
AAACATTTAGATATGATGCTCATCXXXXXXXXXXTTCTTGTATATGTTGGATAAATTATC
AGAACCAGACCTATAAACCAAATCCCAGTTTTATGTCGTGCATTGGAAATTTTATTCATT
CTTCATGCAGATCATGAATTAAATTGTTCAACCGCTGCAATGAGACATATTTCATCATCA
GGTACCGATCCATACACAAGTGTTGCTGGTGCCGCTGGTGCATTATATGGTCCATCTCAT
GGTGGTGCCAATGAGGCCGTATTGGATATGTTAATTCATATCGGTTCCAAAGAGAATATT
CCACAATTCATTTCAGATGTAAAGAGCAAAAAGAAGAAATTAATGGGTTTCGGTCATAGA
ATCTACAAAAACTATGATCCAAGAGCCAAGATCATTCGTAGAGTAGCTTATGAAGTTTTC
GAAAGTTTAGGTAAAGAACCATTGATTGAAGTAGCCACTGAGTTGGAGAAACAAGCCTTG
GAAGATGAATACTTTGTAAGCAGAAAACTTTATCCAAATGTTGATTTCTACTCTGGTCTC
ATCTATAAAGCAATGGGTTTCCCAACTGATATGTTCCCAGTTTTATTCACTATTCCACGT
GCCGTTGGTTGGTTAGCTCATTGGGTAGAACATTTAGAAGATCCAGAAACTAAAATCTAT
CGTCCACGTCAAGTTTACAAAGGTGAATGGTTTAGAAACTATGTCCCAATCGATGGTAGA
CCACCAGCTAAAGTTCGTTCTCAGGAAGTTATTCGTCTGCTACCACTAAAAGATATTCAA
AAGTACTTCTCATNAAATTTTAAAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
llaywxlnt*fifiil*IYIFNMAHIDRINILANHLSKEEEEENELLTSPVSADKKKETV
TVTDNRNGKSYDFKIKNDTINALNFKELQLAKGDGGLMIYDPGFQNTAVVTSYITYIDGD
KGILRYRGYPIEELAERSNFLEVAYLLINGNLPNKSQLDGWSNKIMTHTFLHENLVGLMK
TFRYDAH---

---SCICWINYQNQTYKPNPSFMSCIGNFIHSSCRS*iklfnrcnetyfiiryrsihkcc
wcrwciiwsiswwcq*grigyvnsyrfqreystihfrckeqkeeingfrs*nlqkl*sks
qdhs*ssl*sfrkfr*rtid*ssh*vgetslgr*ilckqktlskc*fllwshl*sngfpn
*yvpsfihystcrwlvsslgrtfrrsrn*nlsstsslqr*mv*klcpnrw*tts*ssfsg
syssattkryskvllxkf*
Translated Amino Acid sequence (All Frames) 
Frame A:
hcwptgi*ihnlyllfykyifliwhilivliylpiifqrkkkkkmny*lhqfllikkkrq
lqlqiiemvkamisklkmtllml*tlknyn*pkvmvv**fmiqvskiqql*hhtlhilmv
ikef*dteviqlkn*qkdqtf*rllif*smvtyqinhn*mvgvikl*lilffmkiwlvl*
khldmmli---

---flymldklsepdl*tksqfyvvhwkfysffmqimn*ivqplq*difhhqvpihtqvl
lvplvhymvhlmvvpmrpywic*fisvpkrifhnsfqm*rakrrn*wvsviestktmiqe
prsfve*lmkfskv*vknh*lk*plswrnkpwkmntl*aenfiqmlistlvssikqwvsq
licsqfyslfhvplvg*lig*ni*kiqklksivhvkftkvngletmsqsmvdhqlkfvlr
klfvcyh*kifkstshxiln

Frame B:
tvgllxfeyiiyiyyfiniyf*ygty*sy*ytcqssfkgrrrrk*iinftsfc**kkrds
ysyr**kw*kl*fqn*k*hy*cfkl*ritisqr*wwfndl*srfpkysscniihyiy*w*
*rnfkiqrlsn*risrkiklfrgclsfnqw*ltk*itirwle**nydsyfss*kfgwsye
ni*i*cs---

---SCICWINYQNQTYKPNPSFMSCIGNFIHSSCRS*iklfnrcnetyfiiryrsihkcc
wcrwciiwsiswwcq*grigyvnsyrfqreystihfrckeqkeeingfrs*nlqkl*sks
qdhs*ssl*sfrkfr*rtid*ssh*vgetslgr*ilckqktlskc*fllwshl*sngfpn
*yvpsfihystcrwlvsslgrtfrrsrn*nlsstsslqr*mv*klcpnrw*tts*ssfsg
syssattkryskvllxkf*

Frame C:
llaywxlnt*fifiil*IYIFNMAHIDRINILANHLSKEEEEENELLTSPVSADKKKETV
TVTDNRNGKSYDFKIKNDTINALNFKELQLAKGDGGLMIYDPGFQNTAVVTSYITYIDGD
KGILRYRGYPIEELAERSNFLEVAYLLINGNLPNKSQLDGWSNKIMTHTFLHENLVGLMK
TFRYDAH---

---lvyvg*iirtrpinqipvlcraleilfilhadhelncstaamrhisssgtdpytsva
gaagalygpshgganeavldmlihigskenipqfisdvkskkkklmgfghriyknydpra
kiirrvayevfeslgkeplievatelekqaledeyfvsrklypnvdfysgliykamgfpt
dmfpvlftipravgwlahwvehledpetkiyrprqvykgewfrnyvpidgrppakvrsqe
virllplkdiqkyfsxnfk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFJ471 (AFJ471Q) /CSM/AF/AFJ4-C/AFJ471Q.Seq.d/                       2359   0.0
AFK171 (AFK171Q) /CSM/AF/AFK1-C/AFK171Q.Seq.d/                       1398   0.0
AFM803 (AFM803Q) /CSM/AF/AFM8-A/AFM803Q.Seq.d/                       1366   0.0
AFN717 (AFN717Q) /CSM/AF/AFN7-A/AFN717Q.Seq.d/                       1356   0.0
AFJ440 (AFJ440Q) /CSM/AF/AFJ4-B/AFJ440Q.Seq.d/                       1346   0.0
AFJ723 (AFJ723Q) /CSM/AF/AFJ7-A/AFJ723Q.Seq.d/                       1336   0.0
AFI655 (AFI655Q) /CSM/AF/AFI6-C/AFI655Q.Seq.d/                       1336   0.0
SSF821 (SSF821Q) /CSM/SS/SSF8-A/SSF821Q.Seq.d/                       1320   0.0
SSK530 (SSK530Q) /CSM/SS/SSK5-B/SSK530Q.Seq.d/                       1300   0.0
SFE393 (SFE393Q) /CSM/SF/SFE3-D/SFE393Q.Seq.d/                       1259   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AY124377|AY124377.1 Dictyostelium discoideum citrate synthase (cshA) mRNA, complete cds.  1312  0.0  6
AC115584|AC115584.2 Dictyostelium discoideum chromosome 2 map complement(3622543-3568102) strain AX4, complete sequence.    80  5e-58  9
AF310892|AF310892.1 Dictyostelium discoideum citrate synthase (gltA) gene, partial cds; RacF2 (racF2) gene, complete cds; and unknown gene.    76  1e-18  2
Z70012|Z70012.1 Bartonella sp. gltA gene (strain N40).            52  3e-12  3
BU044708|BU044708.1 PP_LEa0020E04f Peach developing fruit mesocarp Prunus persica cDNA clone PP_LEa0020E04f, mRNA sequence.    62  3e-10  2
BU040616|BU040616.1 PP_LEa0006L02f Peach developing fruit mesocarp Prunus persica cDNA clone PP_LEa0006L02f, mRNA sequence.    62  3e-10  2
AI895330|AI895330.1 EST264773 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC7M24, mRNA sequence.    46  1e-09  3
Z70011|Z70011.1 Bartonella sp. gltA gene (strain R-phy2).         52  2e-09  3
Z70022|Z70022.1 Bartonella sp. gltA gene (strain C1phy).          52  2e-09  3
BE437000|BE437000.1 EST408118 tomato breaker fruit, TIGR Lycopersicon esculentum cDNA clone cLEG35K15, mRNA sequence.    46  2e-09  3
dna update 2003. 9. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK120755_1(AK120755|pid:none) Oryza sativa Japonica Group cDNA c...   322   1e-86
AB109907_1(AB109907|pid:none) Tetrahymena thermophila tgCS mRNA ...   318   3e-85
AB334779_1(AB334779|pid:none) Glycine max mRNA for peroxisomal c...   312   2e-83
(Q9SJH7) RecName: Full=Citrate synthase 3, peroxisomal;         ...   311   3e-83
(P49299) RecName: Full=Citrate synthase, glyoxysomal;          E...   311   4e-83
CP000096_332(CP000096|pid:none) Pelodictyon luteolum DSM 273, co...   308   3e-82
CP000607_394(CP000607|pid:none) Chlorobium phaeovibrioides DSM 2...   308   4e-82
AB167269_1(AB167269|pid:none) Chlorobium limicola cit gene for c...   306   1e-81
CP001099_363(CP001099|pid:none) Chlorobaculum parvum NCIB 8327, ...   306   1e-81
CP001110_2246(CP001110|pid:none) Pelodictyon phaeoclathratiforme...   306   1e-81
protein update 2009. 4.23
PSORT 

 psg: 0.76  gvh: 0.30  alm: 0.50  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 36.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: endoplasmic reticulum

>> prediction for AFJ471 is nuc
5' end seq. ID AFJ471F
5' end seq. 
>AFJ471F.Seq
CACTGTTGGCCTACTGGNATTTGAATACATAATTTATATTTATTATTTTATAAATATATA
TTTTTAATATGGCACATATTGATCGTATTAATATACTTGCCAATCATCTTTCAAAGGAAG
AAGAAGAAGAAAATGAATTATTAACTTCACCAGTTTCTGCTGATAAAAAAAAAGAGACAG
TTACAGTTACAGATAATAGAAATGGTAAAAGCTATGATTTCAAAATTAAAAATGACACTA
TTAATGCTTTAAACTTTAAAGAATTACAATTAGCCAAAGGTGATGGTGGTTTAATGATTT
ATGATCCAGGTTTCCAAAATACAGCAGTTGTAACATCATACATTACATATATTGATGGTG
ATAAAGGAATTTTAAGATACAGAGGTTATCCAATTGAAGAATTAGCAGAAAGATCAAACT
TTTTAGAGGTTGCTTATCTTTTAATCAATGGTAACTTACCAAATAAATCACAATTAGATG
GTTGGAGTAATAAAATTATGACTCATACTTTTCTTCATGAAAATTTGGTTGGTCTTATGA
AAACATTTAGATATGATGCTCATC----------
Length of 5' end seq. 564
3' end seq. ID AFJ471Z
3' end seq. 
>AFJ471Z.Seq
----------TTCTTGTATATGTTGGATAAATTATCAGAACCAGACCTATAAACCAAATC
CCAGTTTTATGTCGTGCATTGGAAATTTTATTCATTCTTCATGCAGATCATGAATTAAAT
TGTTCAACCGCTGCAATGAGACATATTTCATCATCAGGTACCGATCCATACACAAGTGTT
GCTGGTGCCGCTGGTGCATTATATGGTCCATCTCATGGTGGTGCCAATGAGGCCGTATTG
GATATGTTAATTCATATCGGTTCCAAAGAGAATATTCCACAATTCATTTCAGATGTAAAG
AGCAAAAAGAAGAAATTAATGGGTTTCGGTCATAGAATCTACAAAAACTATGATCCAAGA
GCCAAGATCATTCGTAGAGTAGCTTATGAAGTTTTCGAAAGTTTAGGTAAAGAACCATTG
ATTGAAGTAGCCACTGAGTTGGAGAAACAAGCCTTGGAAGATGAATACTTTGTAAGCAGA
AAACTTTATCCAAATGTTGATTTCTACTCTGGTCTCATCTATAAAGCAATGGGTTTCCCA
ACTGATATGTTCCCAGTTTTATTCACTATTCCACGTGCCGTTGGTTGGTTAGCTCATTGG
GTAGAACATTTAGAAGATCCAGAAACTAAAATCTATCGTCCACGTCAAGTTTACAAAGGT
GAATGGTTTAGAAACTATGTCCCAATCGATGGTAGACCACCAGCTAAAGTTCGTTCTCAG
GAAGTTATTCGTCTGCTACCACTAAAAGATATTCAAAAGTACTTCTCATNAAATTTTAAA
T
Length of 3' end seq. 771
Connected seq. ID AFJ471P
Connected seq. 
>AFJ471P.Seq
CACTGTTGGCCTACTGGNATTTGAATACATAATTTATATTTATTATTTTATAAATATATA
TTTTTAATATGGCACATATTGATCGTATTAATATACTTGCCAATCATCTTTCAAAGGAAG
AAGAAGAAGAAAATGAATTATTAACTTCACCAGTTTCTGCTGATAAAAAAAAAGAGACAG
TTACAGTTACAGATAATAGAAATGGTAAAAGCTATGATTTCAAAATTAAAAATGACACTA
TTAATGCTTTAAACTTTAAAGAATTACAATTAGCCAAAGGTGATGGTGGTTTAATGATTT
ATGATCCAGGTTTCCAAAATACAGCAGTTGTAACATCATACATTACATATATTGATGGTG
ATAAAGGAATTTTAAGATACAGAGGTTATCCAATTGAAGAATTAGCAGAAAGATCAAACT
TTTTAGAGGTTGCTTATCTTTTAATCAATGGTAACTTACCAAATAAATCACAATTAGATG
GTTGGAGTAATAAAATTATGACTCATACTTTTCTTCATGAAAATTTGGTTGGTCTTATGA
AAACATTTAGATATGATGCTCATC----------TTCTTGTATATGTTGGATAAATTATC
AGAACCAGACCTATAAACCAAATCCCAGTTTTATGTCGTGCATTGGAAATTTTATTCATT
CTTCATGCAGATCATGAATTAAATTGTTCAACCGCTGCAATGAGACATATTTCATCATCA
GGTACCGATCCATACACAAGTGTTGCTGGTGCCGCTGGTGCATTATATGGTCCATCTCAT
GGTGGTGCCAATGAGGCCGTATTGGATATGTTAATTCATATCGGTTCCAAAGAGAATATT
CCACAATTCATTTCAGATGTAAAGAGCAAAAAGAAGAAATTAATGGGTTTCGGTCATAGA
ATCTACAAAAACTATGATCCAAGAGCCAAGATCATTCGTAGAGTAGCTTATGAAGTTTTC
GAAAGTTTAGGTAAAGAACCATTGATTGAAGTAGCCACTGAGTTGGAGAAACAAGCCTTG
GAAGATGAATACTTTGTAAGCAGAAAACTTTATCCAAATGTTGATTTCTACTCTGGTCTC
ATCTATAAAGCAATGGGTTTCCCAACTGATATGTTCCCAGTTTTATTCACTATTCCACGT
GCCGTTGGTTGGTTAGCTCATTGGGTAGAACATTTAGAAGATCCAGAAACTAAAATCTAT
CGTCCACGTCAAGTTTACAAAGGTGAATGGTTTAGAAACTATGTCCCAATCGATGGTAGA
CCACCAGCTAAAGTTCGTTCTCAGGAAGTTATTCGTCTGCTACCACTAAAAGATATTCAA
AAGTACTTCTCATNAAATTTTAAAT
Length of connected seq. 1335
Full length Seq ID -
Full length Seq. -
Length of full length seq. -