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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFJ459
Library AF
(Link to library)
Clone ID AFJ459
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U02846-1
Original site URL
Representative seq. ID AFJ459P
(Link to Original site)
Representative DNA sequence 
>AFJ459 (AFJ459Q) /CSM/AF/AFJ4-C/AFJ459Q.Seq.d/
AATTTTTTTTTTTTTAATATATTNGCAAATATNCAATNGTAAAGCAAATAAATTTATTCC
ATNTTTTAAGAAATAATAATAATAATAATTAATAATTAAAATCATTTTTTAAAAANGGTA
ANGGGAAATAATAATAATAAAAGNGGTNGCAATAATAATAATAATAATAATAATAGTAAT
AATAAAAAATTAGATAGNCANGTTAAAGCATCATTAANAAAAAGAAANGAGAGATTAAAN
CATGGNGATGAATCATGGAAAAGAGGTNTAGTTAAATTTCAXXXXXXXXXXTCATTTATC
ATATCATAATGATGAACATTATAATTCTGTAAGACCTGCAAATCAATCATTATATCCAAC
AAAATCAACCAGTTCTTCACCAGTTCCAAATTTTGATAATAAACAAACTTTTCAACCTTC
TTCAAAGGGTGGTAAGAATAATAATAATGGTAAAAATAATGGTAAAAATAATGGTGGTGG
TTCATTTGATTATAATAATAATAATGGTAGTAGTTCATTTGATTATAATAATAGTGGTGG
TAATAGTGGAAATTTTATAGACGATTCAGTTTATGAAATTATGACATTAACAGGTGAAAC
GAGTGTAAAAAATATAAAAGAAGCATTAATCGATTGTAATTATAATATAGAAGCAGCTGT
TGAATATTTACTTGCTTTATCATTAAGTAAAGATGATTCAGTTGTTGAAAATAGCAGTAG
TAATAATTTTGATGATAATAGTAGTGTAACTACTACTGATGAAAATGAAATTGATGAAAA
TAAAGATGATAATATAAATACTACCACTACGACAACAACTACCACTACAACTACCACCAC
TACTACAAGTAATAATAATAATAACAATGAAAAAGCTTCAAGAAATCCATATAAAGGTAT
TAAAAAGAAAGAGAATGAAGAGAAAAAAGAAAATCAAGATGAAAGTAATGCACATTTAAG
TAATAAACAAAGAAAAAATTTAAAACAACAACAAAATACAAAACCAAGGAGAGAAGTTCA
CCATATGATAGAAATTCAATCGTNACGACTCNGAGTCAATTGGGTTCT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
nffffnixanxqx*sk*iysxf*eiiiiiinn*NHFLKXVXGNNNNKXGXNNNNNNNNSN
NKKLDXXVKASLXKRXERLXHGDESWKRGXVKF---

---HLSYHNDEHYNSVRPANQSLYPTKSTSSSPVPNFDNKQTFQPSSKGGKNNNNGKNNG
KNNGGGSFDYNNNNGSSSFDYNNSGGNSGNFIDDSVYEIMTLTGETSVKNIKEALIDCNY
NIEAAVEYLLALSLSKDDSVVENSSSNNFDDNSSVTTTDENEIDENKDDNINTTTTTTTT
TTTTTTTTSNNNNNNEKASRNPYKGIKKKENEEKKENQDESNAHLSNKQRKNLKQQQNTK
PRREVHHMIEIQSXRLXVNWV
Translated Amino Acid sequence (All Frames) 
Frame A:
nffffnixanxqx*sk*iysxf*eiiiiiinn*NHFLKXVXGNNNNKXGXNNNNNNNNSN
NKKLDXXVKASLXKRXERLXHGDESWKRGXVKF---

---sfiis***tl*fcktcksiiisnkinqfftsskf***tnfstffkgw*e***w*k*w
*k*wwwfi*l****w**fi*l***ww**wkfyrrfsl*nydinr*neckkykrsinrl*l
*yrssc*iftcfiik*r*fsc*k*q***f*****cnyy**k*n**k*r**ykyyhydnny
hynyhhyyk*****q*ksfkksi*ry*kere*rekrksr*k*ctfk**tkkkfktttkyk
tkersspydrnsivttxsqlgs

Frame B:
ifffliyxqixnxkankfipxfkk*****liikiif*kx*xeiiiikxvaiiiiiiiivi
ikn*ixxlkhh*xkexrd*xmxmnhgkev*lnf---

---HLSYHNDEHYNSVRPANQSLYPTKSTSSSPVPNFDNKQTFQPSSKGGKNNNNGKNNG
KNNGGGSFDYNNNNGSSSFDYNNSGGNSGNFIDDSVYEIMTLTGETSVKNIKEALIDCNY
NIEAAVEYLLALSLSKDDSVVENSSSNNFDDNSSVTTTDENEIDENKDDNINTTTTTTTT
TTTTTTTTSNNNNNNEKASRNPYKGIKKKENEEKKENQDESNAHLSNKQRKNLKQQQNTK
PRREVHHMIEIQSXRLXVNWV

Frame C:
ffff*yixkyxxvkqinlfhxlrnnnnnn**lksffkxgxgk****kxxq**********
*kir*xx*siixkkkxeikxwx*imekrxs*is---

---iyhiimmniiil*dlqinhyiqqnqpvlhqfqiliinklfnllqrvvriiimvkimv
kimvvvhliiiiimvvvhliiiivvviveil*tiqfmkl*h*qvkrv*ki*kkh*sivii
i*kqllniyllyh*vkmiqllkiavviilmiivv*lllmkmklmkikmii*ilplrqqlp
lqlppllqviiiitmkklqeihikvlkrkrmkrkkkikmkvmhi*vinkeki*nnnkiqn
qgekfti**kfnrxdsesigf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SSK512 (SSK512Q) /CSM/SS/SSK5-A/SSK512Q.Seq.d/                        575   e-163
AFJ459 (AFJ459Q) /CSM/AF/AFJ4-C/AFJ459Q.Seq.d/                        575   e-163
CFE504 (CFE504Q) /CSM/CF/CFE5-A/CFE504Q.Seq.d/                        402   e-111
VSH638 (VSH638Q) /CSM/VS/VSH6-B/VSH638Q.Seq.d/                         52   2e-05
SHJ559 (SHJ559Q) /CSM/SH/SHJ5-C/SHJ559Q.Seq.d/                         52   2e-05
CHN329 (CHN329Q) /CSM/CH/CHN3-B/CHN329Q.Seq.d/                         52   2e-05
CHK501 (CHK501Q) /CSM/CH/CHK5-A/CHK501Q.Seq.d/                         52   2e-05
CHE614 (CHE614Q) /CSM/CH/CHE6-A/CHE614Q.Seq.d/                         52   2e-05
AHQ796 (AHQ796Q) /CSM/AH/AHQ7-D/AHQ796Q.Seq.d/                         52   2e-05
AHQ174 (AHQ174Q) /CSM/AH/AHQ1-D/AHQ174Q.Seq.d/                         52   2e-05
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

M59474|M59474.1 Plasmodium falciparum asparagine-rich antigen Pfa35-2 DNA, partial cds.    42  2e-05  4
AE014821|AE014821.1 Plasmodium falciparum 3D7 chromosome 14 section 6 of 13 of the complete sequence.    52  3e-05  4
AL844505|AL844505.1 Plasmodium falciparum chromosome  6.          42  6e-04  5
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.    42  0.002  3
AL109814|AL109814.1 Plasmodium falciparum DNA *** SEQUENCING IN PROGRESS *** from  MAL13P11.    36  0.003  4
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    44  0.005  4
AC007051|AC007051.3 Homo sapiens chromosome 3, clone hRPK.44_A_1, complete sequence.    40  0.006  4
AC116988|AC116988.2 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    42  0.006  5
AC007919|AC007919.18 Homo sapiens 3q26.2-27 BAC RPCI11-408H1 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    40  0.007  4
AY232271|AY232271.1 Dictyostelium discoideum putative protein Roco9 (roco9) gene, complete cds.    40  0.010  3
dna update 2003. 9. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC115599_3(AC115599|pid:none) Dictyostelium discoideum chromosom...    38   0.50
(Q54HD2) RecName: Full=Probable serine/threonine-protein kinase ...    35   4.2
FM992689_197(FM992689|pid:none) Candida dubliniensis CD36 chromo...    34   9.4
AP008971_865(AP008971|pid:none) Finegoldia magna ATCC 29328 DNA,...    34   9.4
(Q54IV3) RecName: Full=Probable ATP-dependent RNA helicase ddx42...    34   9.4
AL031745_93(AL031745|pid:none) Plasmodium falciparum DNA from MA...    34   9.4
(Q54R73) RecName: Full=Uncharacterized protein DDB_G0283357;           34   9.4
protein update 2009. 4.23
PSORT 

 psg: 0.59  gvh: 0.32  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.10  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 28.0 %: cytoplasmic
	 20.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for AFJ459 is nuc
5' end seq. ID AFJ459F
5' end seq. 
>AFJ459F.Seq
AATTTTTTTTTTTTTAATATATTNGCAAATATNCAATNGTAAAGCAAATAAATTTATTCC
ATNTTTTAAGAAATAATAATAATAATAATTAATAATTAAAATCATTTTTTAAAAANGGTA
ANGGGAAATAATAATAATAAAAGNGGTNGCAATAATAATAATAATAATAATAATAGTAAT
AATAAAAAATTAGATAGNCANGTTAAAGCATCATTAANAAAAAGAAANGAGAGATTAAAN
CATGGNGATGAATCATGGAAAAGAGGTNTAGTTAAATTTCA----------
Length of 5' end seq. 281
3' end seq. ID AFJ459Z
3' end seq. 
>AFJ459Z.Seq
----------TCATTTATCATATCATAATGATGAACATTATAATTCTGTAAGACCTGCAA
ATCAATCATTATATCCAACAAAATCAACCAGTTCTTCACCAGTTCCAAATTTTGATAATA
AACAAACTTTTCAACCTTCTTCAAAGGGTGGTAAGAATAATAATAATGGTAAAAATAATG
GTAAAAATAATGGTGGTGGTTCATTTGATTATAATAATAATAATGGTAGTAGTTCATTTG
ATTATAATAATAGTGGTGGTAATAGTGGAAATTTTATAGACGATTCAGTTTATGAAATTA
TGACATTAACAGGTGAAACGAGTGTAAAAAATATAAAAGAAGCATTAATCGATTGTAATT
ATAATATAGAAGCAGCTGTTGAATATTTACTTGCTTTATCATTAAGTAAAGATGATTCAG
TTGTTGAAAATAGCAGTAGTAATAATTTTGATGATAATAGTAGTGTAACTACTACTGATG
AAAATGAAATTGATGAAAATAAAGATGATAATATAAATACTACCACTACGACAACAACTA
CCACTACAACTACCACCACTACTACAAGTAATAATAATAATAACAATGAAAAAGCTTCAA
GAAATCCATATAAAGGTATTAAAAAGAAAGAGAATGAAGAGAAAAAAGAAAATCAAGATG
AAAGTAATGCACATTTAAGTAATAAACAAAGAAAAAATTTAAAACAACAACAAAATACAA
AACCAAGGAGAGAAGTTCACCATATGATAGAAATTCAATCGTNACGACTCNGAGTCAATT
GGGTTCT
Length of 3' end seq. 777
Connected seq. ID AFJ459P
Connected seq. 
>AFJ459P.Seq
AATTTTTTTTTTTTTAATATATTNGCAAATATNCAATNGTAAAGCAAATAAATTTATTCC
ATNTTTTAAGAAATAATAATAATAATAATTAATAATTAAAATCATTTTTTAAAAANGGTA
ANGGGAAATAATAATAATAAAAGNGGTNGCAATAATAATAATAATAATAATAATAGTAAT
AATAAAAAATTAGATAGNCANGTTAAAGCATCATTAANAAAAAGAAANGAGAGATTAAAN
CATGGNGATGAATCATGGAAAAGAGGTNTAGTTAAATTTCA----------TCATTTATC
ATATCATAATGATGAACATTATAATTCTGTAAGACCTGCAAATCAATCATTATATCCAAC
AAAATCAACCAGTTCTTCACCAGTTCCAAATTTTGATAATAAACAAACTTTTCAACCTTC
TTCAAAGGGTGGTAAGAATAATAATAATGGTAAAAATAATGGTAAAAATAATGGTGGTGG
TTCATTTGATTATAATAATAATAATGGTAGTAGTTCATTTGATTATAATAATAGTGGTGG
TAATAGTGGAAATTTTATAGACGATTCAGTTTATGAAATTATGACATTAACAGGTGAAAC
GAGTGTAAAAAATATAAAAGAAGCATTAATCGATTGTAATTATAATATAGAAGCAGCTGT
TGAATATTTACTTGCTTTATCATTAAGTAAAGATGATTCAGTTGTTGAAAATAGCAGTAG
TAATAATTTTGATGATAATAGTAGTGTAACTACTACTGATGAAAATGAAATTGATGAAAA
TAAAGATGATAATATAAATACTACCACTACGACAACAACTACCACTACAACTACCACCAC
TACTACAAGTAATAATAATAATAACAATGAAAAAGCTTCAAGAAATCCATATAAAGGTAT
TAAAAAGAAAGAGAATGAAGAGAAAAAAGAAAATCAAGATGAAAGTAATGCACATTTAAG
TAATAAACAAAGAAAAAATTTAAAACAACAACAAAATACAAAACCAAGGAGAGAAGTTCA
CCATATGATAGAAATTCAATCGTNACGACTCNGAGTCAATTGGGTTCT
Length of connected seq. 1058
Full length Seq ID -
Full length Seq. -
Length of full length seq. -