>SHG666 (SHG666Q) /CSM/SH/SHG6-C/SHG666Q.Seq.d/
GTTAATAGCAAAAGAAAAATGGCAACAAAAAATACATCTCCTTATGAGATTGAATATGCA
AAGAGTGATAGATCAACATGTTCAACCTGTCAAAGAGGTATTAATAAAGAAGCAGTTCGT
ATTGGTTATAAAACAAAATCAAAACACTTTGATGGAATGGATGTATCATGGCATCATTTA
AAATGTAAATGTCCACAAGTACCATCATTTACAGATTTAATTCACTGGGAATACCTTCGT
TGGGAAGACCAATTATCAATTAAAACAACTTATTTTTCATCTGAAAAGTATGATCCAAAA
TCAGCATCAGAGGTACAAAGAGAGAAATATTTAAAGGCATTATGGGAAGTTAAAGATAGT
ATTGCCGATAACTTGAAGGGACCAGCAATTAAAAACATCATTCAATATAATAAAGGTTAT
GTTGATAAGGTATCACCAGTTCATTTACTTCATACTCTCTCTGATTGGATGTTAAAAGGT
CGTCCAGGTAGATGTCCAACTTGTAAGAATTTCGATATACTTTTCAATGGTATCGAATAT
CAATGTAAAGGTTGGATTTCAGGTTTCACAAAATGTGATTGGAAAGGTGATTCAATTGAA
AGATGXXXXXXXXXXCCACTTGATAAAGATAGTGTAGTTTATAAAACCTTGGTAGATTAT
GTTGCCAATAGTCAAGATAAACAATTTAGAACCAATATCTGTGTTCAAGATATTCTTTCA
CTTGAGAGAGAGAATGAAAGTGAACGTTTCACTCCTTGGGCAAAAGATAAGAATAGATTA
CTCTTATGGCATGGTAGTAGGTTAACTAATTTCGTATCGATTGTCTCGCAGGGATTAAGA
ATTGCTCCACCATCTGCTCCAAAAACTGGTTACAGATTTGGTAAAGGTATTTATTTTGCT
GATTGTATTAGTAAATCATTCAGCTATTGTTTCACATCAAGTGATTGTCCAACTGCACTT
ATGTTATTATGTGAAGTTTCACTTGGTGACATGAATGAATTGAAACATGATACCTATATG
GAAGAGGCACCACATCCATTCCATAGTACAAAAGCTTTAGGTATGGCTGCTCCTCACAAA
GATGGTAATCATCCTCTTTCTGATTCTGATGGTTTAGTAGTACCATTAGGTAAAATTTCA
AAGACTGGTCTCTCAACTTCATGTACTCACAATGAATTCATTGTCTATAAAATTGAACAA
GTCAGAATCAAATACATCTTAAGAGTTTACAANGAAAATAAAATAAAATAAAATTTTTTA
AAATTATCCTTAA

VNSKRKMATKNTSPYEIEYAKSDRSTCSTCQRGINKEAVRIGYKTKSKHFDGMDVSWHHL
KCKCPQVPSFTDLIHWEYLRWEDQLSIKTTYFSSEKYDPKSASEVQREKYLKALWEVKDS
IADNLKGPAIKNIIQYNKGYVDKVSPVHLLHTLSDWMLKGRPGRCPTCKNFDILFNGIEY
QCKGWISGFTKCDWKGDSIER---

---PLDKDSVVYKTLVDYVANSQDKQFRTNICVQDILSLERENESERFTPWAKDKNRLLL
WHGSRLTNFVSIVSQGLRIAPPSAPKTGYRFGKGIYFADCISKSFSYCFTSSDCPTALML
LCEVSLGDMNELKHDTYMEEAPHPFHSTKALGMAAPHKDGNHPLSDSDGLVVPLGKISKT
GLSTSCTHNEFIVYKIEQVRIKYILRVYXENKIK*nflklsl

Frame A:
VNSKRKMATKNTSPYEIEYAKSDRSTCSTCQRGINKEAVRIGYKTKSKHFDGMDVSWHHL
KCKCPQVPSFTDLIHWEYLRWEDQLSIKTTYFSSEKYDPKSASEVQREKYLKALWEVKDS
IADNLKGPAIKNIIQYNKGYVDKVSPVHLLHTLSDWMLKGRPGRCPTCKNFDILFNGIEY
QCKGWISGFTKCDWKGDSIER---

---PLDKDSVVYKTLVDYVANSQDKQFRTNICVQDILSLERENESERFTPWAKDKNRLLL
WHGSRLTNFVSIVSQGLRIAPPSAPKTGYRFGKGIYFADCISKSFSYCFTSSDCPTALML
LCEVSLGDMNELKHDTYMEEAPHPFHSTKALGMAAPHKDGNHPLSDSDGLVVPLGKISKT
GLSTSCTHNEFIVYKIEQVRIKYILRVYXENKIK*nflklsl

Frame B:
liakekwqqkihllmrlnmqrvidqhvqpvkevlikkqfvlvikqnqntlmewmyhgii*
nvnvhkyhhlqi*ftgntfvgktnyqlkqlifhlksmiqnqhqrykerni*rhygklkiv
lpit*rdqqlktsfniikvmliryhqfiyfilsligc*kvvqvdvqlvrisiyfsmvsni
nvkvgfqvsqnvigkviqlkd---

---hlikiv*fikpw*imlpivkinnlepisvfkiffhlrermkvnvsllgqkiridysy
gmvvg*lisyrlsrrd*ellhhllqklvtdlvkvfillivlvnhsaivshqvivqlhlcy
yvkfhlvt*mn*nmipiwkrhhihsivqkl*vwllltkmviilflilmv**yh*vkfqrl
vsqlhvltmnslsiklnksesnts*eftxkik*nkif*nyp*

Frame C:
**qkkngnkkyisl*d*icke**inmfnlskry**rsssywl*nkiktl*wngcimasfk
m*mststiiyrfnslgipslgrpiin*nnlffi*kv*skisirgtkreifkgimgs*r*y
cr*legtsn*khhsi**rlc**gitssftsysl*ldvkrssr*msnl*efrytfqwyris
m*rldfrfhkm*ler*fn*km---

---t**r*csl*nlgrlccq*sr*ti*nqylcsrysft*ere*k*tfhslgkr*e*itlm
aw**vn*fridclagikncsticsknwlqiw*rylfc*ly**iiqllfhik*lsnctyvi
m*sftw*he*iet*ylygrgttsip*yksfrygcssqrw*sssf*f*wfsstir*nfkdw
slnfmysq*ihcl*n*tsqnqihlkslqxk*nkikffkiil

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHG666 (SHG666Q) /CSM/SH/SHG6-C/SHG666Q.Seq.d/                       2383   0.0
VHD227 (VHD227Q) /CSM/VH/VHD2-B/VHD227Q.Seq.d/                       1223   0.0
VHC836 (VHC836Q) /CSM/VH/VHC8-B/VHC836Q.Seq.d/                       1223   0.0
VHB869 (VHB869Q) /CSM/VH/VHB8-C/VHB869Q.Seq.d/                       1223   0.0
VHB303 (VHB303Q) /CSM/VH/VHB3-A/VHB303Q.Seq.d/                       1223   0.0
VHA783 (VHA783Q) /CSM/VH/VHA7-D/VHA783Q.Seq.d/                       1223   0.0
VHA675 (VHA675Q) /CSM/VH/VHA6-D/VHA675Q.Seq.d/                       1223   0.0
VHA575 (VHA575Q) /CSM/VH/VHA5-D/VHA575Q.Seq.d/                       1223   0.0
SHK889 (SHK889Q) /CSM/SH/SHK8-D/SHK889Q.Seq.d/                       1223   0.0
SHJ664 (SHJ664Q) /CSM/SH/SHJ6-C/SHJ664Q.Seq.d/                       1223   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AJ534948|AJ534948.1 Dictyostelium discoideum mRNA for NAD(+) ADP-ribosyltransferase-1A (adprt-1A gene).  1215   0.0    1
AJ520099|AJ520099.1 Dictyostelium discoideum mRNA for (ADP-ribosyl)transferase (adprt2  gene).    62   3e-14  3
DY229813|DY229813.1 ID0AAK8YC07CM1 ApHL3SD Acyrthosiphon pisum cDNA clone ID0AAK8YC07 5', mRNA sequence.    60   1e-04  1
AC025807|AC025807.2 Homo sapiens clone RP11-657G2, WORKING DRAFT SEQUENCE, 15 unordered pieces.    50   0.13   1
AM113503|AM113503.1 Streptococcus sp. 2410 lytA gene for N-acetylmuramoyl-L-alanine  amidase, strain 2410.    50   0.13   1
AM113502|AM113502.1 Streptococcus sp. 3137 lytA gene for N-acetylmuramoyl-L-alanine  amidase, strain 3137.    50   0.13   1
AM113501|AM113501.1 Streptococcus sp. 1956 lytA gene for N-acetylmuramoyl-L-alanine  amidase, strain 1956.    50   0.13   1
AM113500|AM113500.1 Streptococcus sp. 1504 lytA gene for N-acetylmuramoyl-L-alanine  amidase, strain 1504.    50   0.13   1
AM113497|AM113497.1 Streptococcus sp. 578 lytA gene for N-acetylmuramoyl-L-alanine  amidase, strain 578.    50   0.13   1
AM113496|AM113496.1 Streptococcus pseudopneumoniae lytA gene for  N-acetylmuramoyl-L-alanine amidase, strain CCUG 48465.    50   0.13   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

PDBF(1A26) MOL_ID: 1; MOLECULE: POLY (ADP-RIBOSE) POLYMERASE; CH...   185   2e-45
(P26446) RecName: Full=Poly [ADP-ribose] polymerase 1;          ...   185   2e-45
X65497_1(X65497|pid:none) R.norvegicus mRNA for poly(ADP-ribose)...   185   3e-45
(P27008) RecName: Full=Poly [ADP-ribose] polymerase 1;          ...   185   3e-45
AK303340_1(AK303340|pid:none) Homo sapiens cDNA FLJ53442 complet...   183   9e-45
(Q9R152) RecName: Full=Poly [ADP-ribose] polymerase 1;          ...   183   1e-44
AK312339_1(AK312339|pid:none) Homo sapiens cDNA, FLJ92658, highl...   182   2e-44
J03473_1(J03473|pid:none) Human poly(ADP-ribose) synthetase mRNA...   182   2e-44
AB384394_1(AB384394|pid:none) Synthetic construct DNA, clone: pF...   182   2e-44
M17081_1(M17081|pid:none) Human placental poly(ADP-ribose) polym...   182   2e-44

 psg: 0.58  gvh: 0.23  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.39  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 24.0 %: cytoplasmic
	 20.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for SHG666 is nuc

>SHG666F.Seq
GTTAATAGCAAAAGAAAAATGGCAACAAAAAATACATCTCCTTATGAGATTGAATATGCA
AAGAGTGATAGATCAACATGTTCAACCTGTCAAAGAGGTATTAATAAAGAAGCAGTTCGT
ATTGGTTATAAAACAAAATCAAAACACTTTGATGGAATGGATGTATCATGGCATCATTTA
AAATGTAAATGTCCACAAGTACCATCATTTACAGATTTAATTCACTGGGAATACCTTCGT
TGGGAAGACCAATTATCAATTAAAACAACTTATTTTTCATCTGAAAAGTATGATCCAAAA
TCAGCATCAGAGGTACAAAGAGAGAAATATTTAAAGGCATTATGGGAAGTTAAAGATAGT
ATTGCCGATAACTTGAAGGGACCAGCAATTAAAAACATCATTCAATATAATAAAGGTTAT
GTTGATAAGGTATCACCAGTTCATTTACTTCATACTCTCTCTGATTGGATGTTAAAAGGT
CGTCCAGGTAGATGTCCAACTTGTAAGAATTTCGATATACTTTTCAATGGTATCGAATAT
CAATGTAAAGGTTGGATTTCAGGTTTCACAAAATGTGATTGGAAAGGTGATTCAATTGAA
AGATGNNNNNNNNNN

>SHG666Z.Seq
NNNNNNNNNNCCACTTGATAAAGATAGTGTAGTTTATAAAACCTTGGTAGATTATGTTGC
CAATAGTCAAGATAAACAATTTAGAACCAATATCTGTGTTCAAGATATTCTTTCACTTGA
GAGAGAGAATGAAAGTGAACGTTTCACTCCTTGGGCAAAAGATAAGAATAGATTACTCTT
ATGGCATGGTAGTAGGTTAACTAATTTCGTATCGATTGTCTCGCAGGGATTAAGAATTGC
TCCACCATCTGCTCCAAAAACTGGTTACAGATTTGGTAAAGGTATTTATTTTGCTGATTG
TATTAGTAAATCATTCAGCTATTGTTTCACATCAAGTGATTGTCCAACTGCACTTATGTT
ATTATGTGAAGTTTCACTTGGTGACATGAATGAATTGAAACATGATACCTATATGGAAGA
GGCACCACATCCATTCCATAGTACAAAAGCTTTAGGTATGGCTGCTCCTCACAAAGATGG
TAATCATCCTCTTTCTGATTCTGATGGTTTAGTAGTACCATTAGGTAAAATTTCAAAGAC
TGGTCTCTCAACTTCATGTACTCACAATGAATTCATTGTCTATAAAATTGAACAAGTCAG
AATCAAATACATCTTAAGAGTTTACAANGAAAATAAAATAAAATAAAATTTTTTAAAATT
ATCCTTAA

>SHG666P.Seq
GTTAATAGCAAAAGAAAAATGGCAACAAAAAATACATCTCCTTATGAGATTGAATATGCA
AAGAGTGATAGATCAACATGTTCAACCTGTCAAAGAGGTATTAATAAAGAAGCAGTTCGT
ATTGGTTATAAAACAAAATCAAAACACTTTGATGGAATGGATGTATCATGGCATCATTTA
AAATGTAAATGTCCACAAGTACCATCATTTACAGATTTAATTCACTGGGAATACCTTCGT
TGGGAAGACCAATTATCAATTAAAACAACTTATTTTTCATCTGAAAAGTATGATCCAAAA
TCAGCATCAGAGGTACAAAGAGAGAAATATTTAAAGGCATTATGGGAAGTTAAAGATAGT
ATTGCCGATAACTTGAAGGGACCAGCAATTAAAAACATCATTCAATATAATAAAGGTTAT
GTTGATAAGGTATCACCAGTTCATTTACTTCATACTCTCTCTGATTGGATGTTAAAAGGT
CGTCCAGGTAGATGTCCAACTTGTAAGAATTTCGATATACTTTTCAATGGTATCGAATAT
CAATGTAAAGGTTGGATTTCAGGTTTCACAAAATGTGATTGGAAAGGTGATTCAATTGAA
AGATG----------CCACTTGATAAAGATAGTGTAGTTTATAAAACCTTGGTAGATTAT
GTTGCCAATAGTCAAGATAAACAATTTAGAACCAATATCTGTGTTCAAGATATTCTTTCA
CTTGAGAGAGAGAATGAAAGTGAACGTTTCACTCCTTGGGCAAAAGATAAGAATAGATTA
CTCTTATGGCATGGTAGTAGGTTAACTAATTTCGTATCGATTGTCTCGCAGGGATTAAGA
ATTGCTCCACCATCTGCTCCAAAAACTGGTTACAGATTTGGTAAAGGTATTTATTTTGCT
GATTGTATTAGTAAATCATTCAGCTATTGTTTCACATCAAGTGATTGTCCAACTGCACTT
ATGTTATTATGTGAAGTTTCACTTGGTGACATGAATGAATTGAAACATGATACCTATATG
GAAGAGGCACCACATCCATTCCATAGTACAAAAGCTTTAGGTATGGCTGCTCCTCACAAA
GATGGTAATCATCCTCTTTCTGATTCTGATGGTTTAGTAGTACCATTAGGTAAAATTTCA
AAGACTGGTCTCTCAACTTCATGTACTCACAATGAATTCATTGTCTATAAAATTGAACAA
GTCAGAATCAAATACATCTTAAGAGTTTACAANGAAAATAAAATAAAATAAAATTTTTTA
AAATTATCCTTAA