>AFJ435 (AFJ435Q) /CSM/AF/AFJ4-B/AFJ435Q.Seq.d/
ATTCAATTTTATTTTTAATCAATTCAAATTAAAAAAAAAAACAATAACTAACTAAGTTAA
AAATAAAAAAATAATAAAGGATGTTGTTTCTAAAAAATATTGGAGTATTTTTTATGATAT
TCCTTGTATCAAAATCCTATGCGACGGATTGTAATAAAATTACTAATGAAGAAGAATGTC
ATAAATCTTCGGAATGTATTGTTATAAATTATACACCATGTTGNGGNGAACAAAAATGGG
CTTGCTNTAAAGGTACATTTGATACTTGTACATATGAAAATAGTTGTTATAGAAATTCAT
CCAATAACCAAGTTGTTGAAGTATCAAATAAATGCTTCAATCTTGATGGATTTATTAAAA
TTACAACTCCAACCGAGTATTCTTGTTCTGATGCCAAGATTAAAGAATGNGCCTTATTAG
GTAAATCATGTAGTTTCCAAAAGAACTCTTGTTCAAATCCAACTTCCTGTTGCCCAGGTG
AATCAATTTGTGAAGGTTTAAGCTCTGGTAGTTCAAXXXXXXXXXX

silflinsn*kkkq*ltklkikk**RMLFLKNIGVFFMIFLVSKSYATDCNKITNEEECH
KSSECIVINYTPCXGEQKWACXKGTFDTCTYENSCYRNSSNNQVVEVSNKCFNLDGFIKI
TTPTEYSCSDAKIKEXALLGKSCSFQKNSCSNPTSCCPGESICEGLSSGSS---

Frame A:
iqfyf*siqikkknnn*ls*k*knnkgccf*kileyfl*yslyqnpmrrivikllmkknv
inlrnvll*iihhvxxnknglaxkvhlilvhmkivvieihpitkllkyqinasilmdllk
lqlqpsilvlmprlknxpy*vnhvvskrtlvqiqlpvaqvnqfvkv*alvv---


Frame B:
fnfifnqfklkkktitn*vknkkiikdvvskkywsifydipcikilcdgl**ny**rrms
*ifgmycyklytmlxxtkmgll*ryi*ylyi*k*ll*kfiq*psc*sik*mlqs*wiy*n
ynsnrvflf*cqd*rmxlir*im*fpkellfksnfllpr*inl*rfklw*f---


Frame C:
silflinsn*kkkq*ltklkikk**RMLFLKNIGVFFMIFLVSKSYATDCNKITNEEECH
KSSECIVINYTPCXGEQKWACXKGTFDTCTYENSCYRNSSNNQVVEVSNKCFNLDGFIKI
TTPTEYSCSDAKIKEXALLGKSCSFQKNSCSNPTSCCPGESICEGLSSGSS---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLG501 (SLG501Q) /CSM/SL/SLG5-A/SLG501Q.Seq.d/                        858   0.0
SFL250 (SFL250Q) /CSM/SF/SFL2-C/SFL250Q.Seq.d/                        858   0.0
SFK426 (SFK426Q) /CSM/SF/SFK4-B/SFK426Q.Seq.d/                        858   0.0
SFK212 (SFK212Q) /CSM/SF/SFK2-A/SFK212Q.Seq.d/                        858   0.0
SFJ633 (SFJ633Q) /CSM/SF/SFJ6-B/SFJ633Q.Seq.d/                        858   0.0
SFJ257 (SFJ257Q) /CSM/SF/SFJ2-C/SFJ257Q.Seq.d/                        858   0.0
SFH201 (SFH201Q) /CSM/SF/SFH2-A/SFH201Q.Seq.d/                        858   0.0
SFH185 (SFH185Q) /CSM/SF/SFH1-D/SFH185Q.Seq.d/                        858   0.0
SFG768 (SFG768Q) /CSM/SF/SFG7-C/SFG768Q.Seq.d/                        858   0.0
SFG663 (SFG663Q) /CSM/SF/SFG6-C/SFG663Q.Seq.d/                        858   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U25144|U25144.1 Dictyostelium discoideum spore coat protein SP87 (PspD) gene, complete cds.   660  0.0  4
AC117267|AC117267.1 Dictyostelium discoideum chromosome 2 map 4767688-4790343 strain AX4, *** SEQUENCING IN PROGRESS ***, in ordered pieces.   660  0.0  4
AC115610|AC115610.2 Dictyostelium discoideum chromosome 2 map 5836255-5862024 strain AX4, complete sequence.   660  0.0  4
CB566403|CB566403.1 AGENCOURT_12971596 NIH_MGC_135 Mus musculus cDNA clone IMAGE:30293960 5', mRNA sequence.    40  0.49  2
AL353814|AL353814.1 Arabidopsis thaliana DNA chromosome 3, BAC clone F26G5.    36  0.55  4
U67535|U67535.1 Methanococcus jannaschii section 77 of 150 of the complete genome.    42  0.98  2
AC120760|AC120760.4 Rattus norvegicus clone CH230-71L4, *** SEQUENCING IN PROGRESS ***, 6 unordered pieces.    44  1.4  2
AC106246|AC106246.3 Rattus norvegicus clone CH230-220G2, *** SEQUENCING IN PROGRESS ***, 9 unordered pieces.    44  1.7  1
AC106231|AC106231.4 Rattus norvegicus clone CH230-105P10, WORKING DRAFT SEQUENCE, 3 unordered pieces.    44  1.7  1
AC098542|AC098542.5 Rattus norvegicus clone CH230-99J13, WORKING DRAFT SEQUENCE, 4 unordered pieces.    44  1.7  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC117267_7(AC117267|pid:none) Dictyostelium discoideum chromosom...   234   7e-61
(Q6TU45) RecName: Full=Probable spore coat protein sigD; AltName...   100   3e-20
(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;        46   5e-04
D13973_1(D13973|pid:none) Dictyostelium discoideum gene for Dp87...    43   0.005
(Q99088) RecName: Full=Low-density lipoprotein receptor 2;      ...    34   1.8
QRXLL2(B40388)LDL receptor 2 precursor - African clawed frog           34   1.8
(Q99087) RecName: Full=Low-density lipoprotein receptor 1;      ...    34   2.4
BC170345_1(BC170345|pid:none) Xenopus laevis low density lipopro...    34   2.4
QRXLL1(A40388)LDL receptor 1 precursor - African clawed frog           34   2.4
(Q07954) RecName: Full=Prolow-density lipoprotein receptor-relat...    33   3.1

 psg: 1.02  gvh: 0.68  alm: 0.51  top: 0.20  tms: 0.00  mit: 0.38  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	 12.0 %: vacuolar
	 12.0 %: endoplasmic reticulum
	  8.0 %: nuclear

>> prediction for AFJ435 is exc

>AFJ435F.Seq
ATTCAATTTTATTTTTAATCAATTCAAATTAAAAAAAAAAACAATAACTAACTAAGTTAA
AAATAAAAAAATAATAAAGGATGTTGTTTCTAAAAAATATTGGAGTATTTTTTATGATAT
TCCTTGTATCAAAATCCTATGCGACGGATTGTAATAAAATTACTAATGAAGAAGAATGTC
ATAAATCTTCGGAATGTATTGTTATAAATTATACACCATGTTGNGGNGAACAAAAATGGG
CTTGCTNTAAAGGTACATTTGATACTTGTACATATGAAAATAGTTGTTATAGAAATTCAT
CCAATAACCAAGTTGTTGAAGTATCAAATAAATGCTTCAATCTTGATGGATTTATTAAAA
TTACAACTCCAACCGAGTATTCTTGTTCTGATGCCAAGATTAAAGAATGNGCCTTATTAG
GTAAATCATGTAGTTTCCAAAAGAACTCTTGTTCAAATCCAACTTCCTGTTGCCCAGGTG
AATCAATTTGTGAAGGTTTAAGCTCTGGTAGTTCAA----------