>SHG555 (SHG555Q) /CSM/SH/SHG5-C/SHG555Q.Seq.d/
ACTTATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCG
TCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAG
TTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAG
GTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACT
TAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCA
TTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTA
CCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCA
GAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTA
CTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCAGGTA
ATAAATTAGCTATGCAAGAATTTATGATTTTACCAGTTGGTGCTAAAGATTTCAATGAAG
CCTATCXXXXXXXXXXTCAATGAAGCCTATCGTATGGGTTCAGAAGTTTATCATAACTTA
AAGAATGTCATCAGTGGTAGATACGGTCAAGATGCTATCAATGTTGGTGATGAAGGTGGT
TTCGCTCCACCAATTCAAAGCAACAAAGAAGGTCTTGAACTCTTAAAACTTGCCATTGAA
AAAGCTGGTTACACAGGTTTAGTTAAAATTGGTATGGATTGTGCTGCCTCTGAATTCAAA
GTTGAAAATGGTTACGATCTTGACTTCAAAACTAAAAACAATGACGGTTCAGCTGTTATC
TCTGGTGAAAAATTAGGTGATCTCTACAGAGAATTCATTAAAGAATATCCAATCATTTCA
ATTGAAGATCCATTTGATCAAGATGATTGGGAATCATACACTAAATTAACAGCATCAGTT
GATATTCAAATTGTTGGTGATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGGT
ATTGAAAAGAAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAAATGGTTCAGTCACTG
AATCAATTCGTGCCGCTCTGACTCAAAGAAGCATCATGGGGTGTCA

LLLYFFLSLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVPSG
ASTGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGT
PNKGKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHAGN
KLAMQEFMILPVGAKDFNEAY---

---NEAYRMGSEVYHNLKNVISGRYGQDAINVGDEGGFAPPIQSNKEGLELLKLAIEKAG
YTGLVKIGMDCAASEFKVENGYDLDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIED
PFDQDDWESYTKLTASVDIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQMVQSLNQF
VPL*lkeaswgv

Frame A:
tyyfiffylylyiyhflk*assnqlkpekf*ihvvtqllkltftpkkmvlfhhselqfhq
vhqlvfmklln*emvinqdt*vkvyskqsktfsksfnqpslvnqfpikllsin**sismv
pqikvn*vptlslqsv*lfaelvllieiyhftnisqklqvlk*dyqfqhsmlsmvvhmqv
in*lcknl*fyqlvlkismkpi---

---smkpivwvqkfiit*rmssvvdtvkmlsmlvmkvvslhqfkatkkvlns*nlplkkl
vtqv*lklvwivlplnsklkmvtiltsklktmtvqllslvkn*vistenslkniqsfqlk
ihlikmignhtln*qhqlifkllvmiyllptqkelrlvlkrkhvmlfy*kltkwfsh*in
scrsdskkhhgvs

Frame B:
litlfffisiyiyiif*nerhqin*sqrnfrftw*pnc*s*plhrkrwccfiiqscssir
cinwyl*sc*ikrw**ikilr*rctqsnqkhsrshstsrhw*isfrsscyr*indrsrwy
pk*r*ircqrypcsqfsclqswcc**kfttlqiylrncry*neitsssiqcyqwwftcr*
*isyariydftswc*rfq*sl---

---q*slsygfrsls*lkechqw*irsrcyqcw**rwfrstnskqqrrs*tlktch*ksw
lhrfs*nwyglccl*iqs*kwlrs*lqn*kq*rfscylw*kir*slqrih*risnhfn*r
si*sr*lgiih*insis*ysncw**ftcyqprkn*dwy*kesm*csfiks*pngsvtesi
raaltqrsimgc

Frame C:
LLLYFFLSLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVPSG
ASTGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGT
PNKGKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHAGN
KLAMQEFMILPVGAKDFNEAY---

---NEAYRMGSEVYHNLKNVISGRYGQDAINVGDEGGFAPPIQSNKEGLELLKLAIEKAG
YTGLVKIGMDCAASEFKVENGYDLDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIED
PFDQDDWESYTKLTASVDIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQMVQSLNQF
VPL*lkeaswgv

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHG555 (SHG555Q) /CSM/SH/SHG5-C/SHG555Q.Seq.d/                       2248   0.0
VFB851 (VFB851Q) /CSM/VF/VFB8-C/VFB851Q.Seq.d/                       1158   0.0
SSM734 (SSM734Q) /CSM/SS/SSM7-B/SSM734Q.Seq.d/                       1158   0.0
SFH339 (SFH339Q) /CSM/SF/SFH3-B/SFH339Q.Seq.d/                       1158   0.0
SFE744 (SFE744Q) /CSM/SF/SFE7-B/SFE744Q.Seq.d/                       1158   0.0
SFB722 (SFB722Q) /CSM/SF/SFB7-A/SFB722Q.Seq.d/                       1158   0.0
CFD322 (CFD322Q) /CSM/CF/CFD3-A/CFD322Q.Seq.d/                       1158   0.0
AFO869 (AFO869Q) /CSM/AF/AFO8-C/AFO869Q.Seq.d/                       1158   0.0
AFO860 (AFO860Q) /CSM/AF/AFO8-C/AFO860Q.Seq.d/                       1158   0.0
AFO841 (AFO841Q) /CSM/AF/AFO8-B/AFO841Q.Seq.d/                       1158   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressinggrowth of plasmodium.    76   5e-56  9
BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76   5e-56  9
AB026051|AB026051.1 Plasmodium falciparum mRNA for enolase, complete cds.    76   5e-56  9
X80474|X80474.1 N.frontalis enolase gene.                          64   4e-55  11
U00152|U00152.1 Plasmodium falciparum enolase gene complete cds.    76   4e-54  8
Y17298|Y17298.1 Cunninghamella elegans mRNA for enolase.           86   4e-54  9
BM165201|BM165201.1 EST567724 PyBS Plasmodium yoelii yoelii cDNA clone PYCMI87 5' end, mRNA sequence.    92   2e-51  5
DY886402|DY886402.1 CeleSEQ2047 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ2047, mRNA sequence.    86   3e-51  8
DY887760|DY887760.1 CeleSEQ4412 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ4412, mRNA sequence.    86   4e-50  8
AY522934|AY522934.1 Artemia sp. SBH266677 2-phospho-D-glycerate hydrolase mRNA, partial cds.    70   8e-48  8

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RK5) RecName: Full=Enolase A;          EC=4.2.1.11; AltName:...   354   0.0
EF137905_1(EF137905|pid:none) Helianthus annuus plastid enolase ...   251   e-133
AC016662_10(AC016662|pid:none) Arabidopsis thaliana chromosome 1...   246   e-131
DQ497988_1(DQ497988|pid:none) Polypterus senegalus enolase B mRN...   245   e-128
AK243111_1(AK243111|pid:none) Oryza sativa Japonica Group cDNA, ...   242   e-127
BC045082_1(BC045082|pid:none) Xenopus laevis enolase 3, beta mus...   250   e-127
BC096516_1(BC096516|pid:none) Xenopus tropicalis hypothetical pr...   249   e-127
T12341(T12341) phosphopyruvate hydratase (EC 4.2.1.11) - common ...   250   e-126
(Q43130) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   249   e-126
EU964132_1(EU964132|pid:none) Zea mays clone 276124 enolase mRNA...   245   e-126

 psg: 0.95  gvh: 0.47  alm: 0.32  top: 0.60  tms: 0.00  mit: 0.36  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 28.0 %: nuclear
	 24.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for SHG555 is cyt

>SHG555F.Seq
ACTTATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCG
TCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAG
TTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAG
GTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACT
TAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCA
TTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTA
CCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCA
GAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTA
CTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCAGGTA
ATAAATTAGCTATGCAAGAATTTATGATTTTACCAGTTGGTGCTAAAGATTTCAATGAAG
CCTATCNNNNNNNNNN

>SHG555Z.Seq
NNNNNNNNNNTCAATGAAGCCTATCGTATGGGTTCAGAAGTTTATCATAACTTAAAGAAT
GTCATCAGTGGTAGATACGGTCAAGATGCTATCAATGTTGGTGATGAAGGTGGTTTCGCT
CCACCAATTCAAAGCAACAAAGAAGGTCTTGAACTCTTAAAACTTGCCATTGAAAAAGCT
GGTTACACAGGTTTAGTTAAAATTGGTATGGATTGTGCTGCCTCTGAATTCAAAGTTGAA
AATGGTTACGATCTTGACTTCAAAACTAAAAACAATGACGGTTCAGCTGTTATCTCTGGT
GAAAAATTAGGTGATCTCTACAGAGAATTCATTAAAGAATATCCAATCATTTCAATTGAA
GATCCATTTGATCAAGATGATTGGGAATCATACACTAAATTAACAGCATCAGTTGATATT
CAAATTGTTGGTGATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGGTATTGAA
AAGAAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAAATGGTTCAGTCACTGAATCAA
TTCGTGCCGCTCTGACTCAAAGAAGCATCATGGGGTGTCA

>SHG555P.Seq
ACTTATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCG
TCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAG
TTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAG
GTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACT
TAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCA
TTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTA
CCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCA
GAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTA
CTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCAGGTA
ATAAATTAGCTATGCAAGAATTTATGATTTTACCAGTTGGTGCTAAAGATTTCAATGAAG
CCTATC----------TCAATGAAGCCTATCGTATGGGTTCAGAAGTTTATCATAACTTA
AAGAATGTCATCAGTGGTAGATACGGTCAAGATGCTATCAATGTTGGTGATGAAGGTGGT
TTCGCTCCACCAATTCAAAGCAACAAAGAAGGTCTTGAACTCTTAAAACTTGCCATTGAA
AAAGCTGGTTACACAGGTTTAGTTAAAATTGGTATGGATTGTGCTGCCTCTGAATTCAAA
GTTGAAAATGGTTACGATCTTGACTTCAAAACTAAAAACAATGACGGTTCAGCTGTTATC
TCTGGTGAAAAATTAGGTGATCTCTACAGAGAATTCATTAAAGAATATCCAATCATTTCA
ATTGAAGATCCATTTGATCAAGATGATTGGGAATCATACACTAAATTAACAGCATCAGTT
GATATTCAAATTGTTGGTGATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGGT
ATTGAAAAGAAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAAATGGTTCAGTCACTG
AATCAATTCGTGCCGCTCTGACTCAAAGAAGCATCATGGGGTGTCA