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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHG549
Library SH
(Link to library)
Clone ID SHG549
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11926-1
Original site URL
Representative seq. ID SHG549P
(Link to Original site)
Representative DNA sequence 
>SHG549 (SHG549Q) /CSM/SH/SHG5-C/SHG549Q.Seq.d/
AATCCATGTACCATCGATGCCTGTACTAAATCAACAGGTGTTACTCATACCCCAGTCAAT
GTAGATGATAATAACAAATGTACAATTGATACATGTACCAAAGAAGGTGGTGTAACTCAT
ACTCCAGTCAATACTGATGATAACAATGCCTGTACCCTTGATTCCTGCTCACCATCAACT
GGTGTTTCCCATACCCCAATAAACTGTGATGATAATAATAAATGTACTGTAGATTCATGT
TCAAACTCAACTGGTTGTGTAAATACTCCAATTAACTGTGACGATAGTAATCCATGTACC
GTAGACTCATGCANTAATTCAACTGGTTGTGTAAACACTCCAGTCAATGTTGATGATAAT
AATCCATGTACCGTCGATGCCTGTACCAAATCAACAGGTGTTACTCATACCCCAGTCAAT
GTAGATGATAACAACAAATGTACAATTGATGCATGTACCAAAGAAGGTGGTGTAACTCAT
ACCCCAGTCAATACTGATGATAACAATGCCTGTACAATTGATGCATGTACAAAAGAAGGT
GGTGTAACTCATACTCCANTCAATACTGATGATAACAATGCTTGTACCCTTGACTCCTGT
TCACCATCAACTGGCGTTTCCCACACNCCAATTAACTGTGATGATAGTAATCCATGTACC
GTAGACTCATGTTCAAATTCAACCGGTTGTTGCAACACTCCAATCAATGGTGATGATAAT
AATCCATGTACTGTAGATTCATGTACCAAACCAACAGGTGGTACTCATACCCCAATCAAT
GGTNGATGATAACAACCAATGTXXXXXXXXXXGNTTTAATTTAATNTCTGGNTTAANTGG
GGGTNTCNTTGGNGGNGGGACNGGANGNAAANGTGATTGCNAAACNTGTAAAAATTAAAN
AACNCNTTTTATTTAATTATATTTTAAAAAAATTCNAAAAAAATTCNAAAAAAAATTCNA
NAAAAAAAAAAAACTATTTGTTTTCTTATCTAATATTTATAATTTTTAAAAATAAAAATT
CATNCCAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
smyhrcly*inrcysypsqcr***qmyn*ymyqrrwcnsyssqy***qclyp*flltinw
cfpypnkl*****mycrfmfklnwlckysn*l*r**smyrrlmx*fnwlckhssqc****
smyrrclyqinrcysypsqcr**qqmyn*cmyqrrwcnsypsqy***qclyn*cmykrrw
cnsysxqy***qclyp*llftinwrfphxn*l****smyrrlmfkfnrllqhsnqw****
SMYCRFMYQTNRWYSYPNQWXMITTN---

---FNLXSGLXGGXXGGGTGXKXDCXTCKN*xtxfi*lyfkkixkkfxkkfxkkkklfvf
lsniynf*k*kfxpk
Translated Amino Acid sequence (All Frames) 
Frame A:
npctidactkstgvthtpvnvddnnkctidtctkeggvthtpvntddnnactldscspst
gvshtpincddnnkctvdscsnstgcvntpincddsnpctvdscxnstgcvntpvnvddn
npctvdactkstgvthtpvnvddnnkctidactkeggvthtpvntddnnactidactkeg
gvthtpxntddnnactldscspstgvshtpincddsnpctvdscsnstgccntpingddn
npctvdsctkptggthtpingx**qpm---

---xli*xlx*xgvslxxgxxxxviakxvkikxxxlfnyilkkfxknskknsxkkknylf
sylifiifknknsxqk

Frame B:
ihvpsmpvlnqqvllipqsm*miitnvqlihvpkkvv*lilqsilmitmpvplipahhql
vfpipq*tvmiiinvl*ihvqtqlvv*ilqltvtivihvp*thaxiqlvv*tlqsmlmii
ihvpsmpvpnqqvllipqsm*mittnvqlmhvpkkvv*lipqsilmitmpvqlmhvqkkv
v*lilxsilmitmlvpltpvhhqlafptxqltvmivihvp*thvqiqpvvatlqsmvmii
ihvl*ihvpnqqvvlipqsmvddnnqc---

---x*fnxwxxwgxxwxxdxxxx*lxnx*klxnxfyliif*knskkixkkixxkkkticf
li*yl*flkikihxk

Frame C:
smyhrcly*inrcysypsqcr***qmyn*ymyqrrwcnsyssqy***qclyp*flltinw
cfpypnkl*****mycrfmfklnwlckysn*l*r**smyrrlmx*fnwlckhssqc****
smyrrclyqinrcysypsqcr**qqmyn*cmyqrrwcnsypsqy***qclyn*cmykrrw
cnsysxqy***qclyp*llftinwrfphxn*l****smyrrlmfkfnrllqhsnqw****
SMYCRFMYQTNRWYSYPNQWXMITTN---

---FNLXSGLXGGXXGGGTGXKXDCXTCKN*xtxfi*lyfkkixkkfxkkfxkkkklfvf
lsniynf*k*kfxpk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHG549 (SHG549Q) /CSM/SH/SHG5-C/SHG549Q.Seq.d/                       1649   0.0
SHA152 (SHA152Q) /CSM/SH/SHA1-C/SHA152Q.Seq.d/                       1477   0.0
SHF321 (SHF321Q) /CSM/SH/SHF3-A/SHF321Q.Seq.d/                       1376   0.0
SHF556 (SHF556Q) /CSM/SH/SHF5-C/SHF556Q.Seq.d/                       1362   0.0
CHN682 (CHN682Q) /CSM/CH/CHN6-D/CHN682Q.Seq.d/                       1306   0.0
SHC589 (SHC589Q) /CSM/SH/SHC5-D/SHC589Q.Seq.d/                       1292   0.0
CHR684 (CHR684Q) /CSM/CH/CHR6-D/CHR684Q.Seq.d/                       1251   0.0
SHA109 (SHA109Q) /CSM/SH/SHA1-A/SHA109Q.Seq.d/                       1247   0.0
SHA313 (SHA313Q) /CSM/SH/SHA3-A/SHA313Q.Seq.d/                       1245   0.0
SHK502 (SHK502Q) /CSM/SH/SHK5-A/SHK502Q.Seq.d/                       1237   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X78948|X78948.1 D.minutum ecmB gene.                               58   1e-14  6
BU494788|BU494788.1 PfESToab67g03.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5', mRNA sequence.    36   0.003  4
CK991559|CK991559.1 EST0111 Eyestalk cDNA library Penaeus monodon cDNA clone ES-N-S03-555-W 5' similar to Histidine-rich glycoprotein precursor (Plasmodium lophurae), mRNA sequence.    30   0.006  4
Y08924|Y08924.1 P.falciparum mRNA for AARP2 protein.               30   0.008  6
BQ739629|BQ739629.1 PfESToab48c05.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to TR:Q9U0J6 Q9U0J6 HYPOTHETICAL 312.5 KD PROTEIN. ;, mRNA sequence.    30   0.010  4
BU497188|BU497188.1 PfESToab56a10.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to TR:Q9SSI6 Q9SSI6 PUTATIVE CELL DIVISION RELATED PROTEIN. ;, mRNA sequence.    36   0.024  3
AC151654|AC151654.3 Dasypus novemcinctus clone VMRC5-37J9, WORKING DRAFT SEQUENCE, 7 ordered pieces.    52   0.026  1
AC151640|AC151640.3 Dasypus novemcinctus clone VMRC5-185N18, WORKING DRAFT SEQUENCE, 11 ordered pieces.    52   0.026  1
DR453650|DR453650.1 CM015E04 Cotton Lambda Zap Express Library Gossypium hirsutum cDNA, mRNA sequence.    38   0.030  3
BX231032|BX231032.1 Danio rerio genomic clone DKEY-254O18, genomic survey sequence.    30   0.063  5
dna update 2006. 4. 1
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A27020(A27020)DIF-induced prestalk pDd63 protein precursor - sli...   414   e-114
(P11976) RecName: Full=Prestalk protein; AltName: Full=Extracell...   399   e-110
A26838(A26838)prestalk protein precursor - slime mold (Dictyoste...   399   e-110
S44208(S44208) extracellular matrix protein B - Dictyostelium mi...   328   1e-88
AC116984_103(AC116984|pid:none) Dictyostelium discoideum chromos...   308   2e-82
AC117072_61(AC117072|pid:none) Dictyostelium discoideum chromoso...   301   2e-80
(Q54CH8) RecName: Full=Protein psiN; Flags: Precursor;                146   9e-34
(Q54C31) RecName: Full=Protein psiR; Flags: Precursor;                132   1e-29
(Q54G85) RecName: Full=Protein psiJ; Flags: Precursor;                128   3e-28
(Q54C32) RecName: Full=Protein psiQ; Flags: Precursor;                112   3e-23
protein update 2009. 4.19
PSORT 

 psg: 0.63  gvh: 0.61  alm: 0.56  top: 0.53  tms: 0.00  mit: 0.33  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: mitochondrial
	 28.0 %: nuclear
	 16.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: endoplasmic reticulum

>> prediction for SHG549 is mit
5' end seq. ID SHG549F
5' end seq. 
>SHG549F.Seq
AATCCATGTACCATCGATGCCTGTACTAAATCAACAGGTGTTACTCATACCCCAGTCAAT
GTAGATGATAATAACAAATGTACAATTGATACATGTACCAAAGAAGGTGGTGTAACTCAT
ACTCCAGTCAATACTGATGATAACAATGCCTGTACCCTTGATTCCTGCTCACCATCAACT
GGTGTTTCCCATACCCCAATAAACTGTGATGATAATAATAAATGTACTGTAGATTCATGT
TCAAACTCAACTGGTTGTGTAAATACTCCAATTAACTGTGACGATAGTAATCCATGTACC
GTAGACTCATGCANTAATTCAACTGGTTGTGTAAACACTCCAGTCAATGTTGATGATAAT
AATCCATGTACCGTCGATGCCTGTACCAAATCAACAGGTGTTACTCATACCCCAGTCAAT
GTAGATGATAACAACAAATGTACAATTGATGCATGTACCAAAGAAGGTGGTGTAACTCAT
ACCCCAGTCAATACTGATGATAACAATGCCTGTACAATTGATGCATGTACAAAAGAAGGT
GGTGTAACTCATACTCCANTCAATACTGATGATAACAATGCTTGTACCCTTGACTCCTGT
TCACCATCAACTGGCGTTTCCCACACNCCAATTAACTGTGATGATAGTAATCCATGTACC
GTAGACTCATGTTCAAATTCAACCGGTTGTTGCAACACTCCAATCAATGGTGATGATAAT
AATCCATGTACTGTAGATTCATGTACCAAACCAACAGGTGGTACTCATACCCCAATCAAT
GGTNGATGATAACAACCAATGTNNNNNNNNNN
Length of 5' end seq. 812
3' end seq. ID SHG549Z
3' end seq. 
>SHG549Z.Seq
NNNNNNNNNNGNTTTAATTTAATNTCTGGNTTAANTGGGGGTNTCNTTGGNGGNGGGACN
GGANGNAAANGTGATTGCNAAACNTGTAAAAATTAAANAACNCNTTTTATTTAATTATAT
TTTAAAAAAATTCNAAAAAAATTCNAAAAAAAATTCNANAAAAAAAAAAAACTATTTGTT
TTCTTATCTAATATTTATAATTTTTAAAAATAAAAATTCATNCCAAAAA
Length of 3' end seq. 229
Connected seq. ID SHG549P
Connected seq. 
>SHG549P.Seq
AATCCATGTACCATCGATGCCTGTACTAAATCAACAGGTGTTACTCATACCCCAGTCAAT
GTAGATGATAATAACAAATGTACAATTGATACATGTACCAAAGAAGGTGGTGTAACTCAT
ACTCCAGTCAATACTGATGATAACAATGCCTGTACCCTTGATTCCTGCTCACCATCAACT
GGTGTTTCCCATACCCCAATAAACTGTGATGATAATAATAAATGTACTGTAGATTCATGT
TCAAACTCAACTGGTTGTGTAAATACTCCAATTAACTGTGACGATAGTAATCCATGTACC
GTAGACTCATGCANTAATTCAACTGGTTGTGTAAACACTCCAGTCAATGTTGATGATAAT
AATCCATGTACCGTCGATGCCTGTACCAAATCAACAGGTGTTACTCATACCCCAGTCAAT
GTAGATGATAACAACAAATGTACAATTGATGCATGTACCAAAGAAGGTGGTGTAACTCAT
ACCCCAGTCAATACTGATGATAACAATGCCTGTACAATTGATGCATGTACAAAAGAAGGT
GGTGTAACTCATACTCCANTCAATACTGATGATAACAATGCTTGTACCCTTGACTCCTGT
TCACCATCAACTGGCGTTTCCCACACNCCAATTAACTGTGATGATAGTAATCCATGTACC
GTAGACTCATGTTCAAATTCAACCGGTTGTTGCAACACTCCAATCAATGGTGATGATAAT
AATCCATGTACTGTAGATTCATGTACCAAACCAACAGGTGGTACTCATACCCCAATCAAT
GGTNGATGATAACAACCAATGT----------GNTTTAATTTAATNTCTGGNTTAANTGG
GGGTNTCNTTGGNGGNGGGACNGGANGNAAANGTGATTGCNAAACNTGTAAAAATTAAAN
AACNCNTTTTATTTAATTATATTTTAAAAAAATTCNAAAAAAATTCNAAAAAAAATTCNA
NAAAAAAAAAAAACTATTTGTTTTCTTATCTAATATTTATAATTTTTAAAAATAAAAATT
CATNCCAAAAA
Length of connected seq. 1021
Full length Seq ID -
Full length Seq. -
Length of full length seq. -