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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHG495
Library SH
(Link to library)
Clone ID SHG495
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16292-1|Contig-U16400-1
Original site URL
Representative seq. ID SHG495P
(Link to Original site)
Representative DNA sequence 
>SHG495 (SHG495Q) /CSM/SH/SHG4-D/SHG495Q.Seq.d/
AAATCCCAGGTGTGTTCACAAAAATAACAACAACACTAAAAAAAGTCTTCATTGTTTTTT
ACTTTTTTTTTTTAATTTTTAATTTTTTATTTAATTTAAACAATATATAAATCAATTAAA
ATAAACNCTCACTCGATAGNGTAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAANTTTTTTNAAAAAAAAAAANGNCCCCCCCCCCNAAAANNTTTTTN
NGGGGGGGGGGGGGNNAANTTNGGGGGGNNAAAAAANNNNAAAAAAAAANTTTTTTNGGG
GNAAAAANNTTTTNNAAAAAAAAXXXXXXXXXXGCTGCAATGAGTTCTTTAACCTCAGGT
GCTGAACGTTCATATATAATGGAAAGAGGTATCACATTGAATACACTAACCAAGGATTTG
GAAATGTTTGTTGAACGTTTCAAACGTGAACATAGAATTGGTCTAATTATTAAGAGTGAA
CTTGCTAGCAACACCTACTCAACTCATTTCATTTATTCACTCTTCAAAGAAGAAGGTAAA
CATTTATTCGATGTCCGTGAATCTATACTCGGTCATTTACAACAAGGTGGTACTCCTTCT
GCAATTGATCGTATCTTTTCAACTCGTTTAATGAATCATTATTATCAATTCTTGGAAAAC
GATCTCAAAGAACATGGTCATCTTCAAATGAATGGTTGTATTGGTTTCATTGATGGTGGT
ATTCATTACACTCCAATGCAAGAAATGATCGAAGAAATGTCTGA
sequence update 2002.10.25
Translated Amino Acid sequence 
ipgvftkitttlkkvfivfyffflifnflfnlnni*in*NKXSLDXVKEKKKKKKKKKKK
KKKKXFXKKKXXPPPXXXFXGGGGXXXGGXKXXKKXFFXGKXXXXKK---

---AAMSSLTSGAERSYIMERGITLNTLTKDLEMFVERFKREHRIGLIIKSELASNTYST
HFIYSLFKEEGKHLFDVRESILGHLQQGGTPSAIDRIFSTRLMNHYYQFLENDLKEHGHL
QMNGCIGFIDGGIHYTPMQEMIEEMS
Translated Amino Acid sequence (All Frames) 
Frame A:
ksqvcsqk*qqh*kksslfftfff*flifyli*tiyksikinxhsix*kkkkkkkkkkkk
kkkkkxfxkkkxxpppkxfxxggggxxxgxkkxxkkxfxgxkxfxkk---

---AAMSSLTSGAERSYIMERGITLNTLTKDLEMFVERFKREHRIGLIIKSELASNTYST
HFIYSLFKEEGKHLFDVRESILGHLQQGGTPSAIDRIFSTRLMNHYYQFLENDLKEHGHL
QMNGCIGFIDGGIHYTPMQEMIEEMS

Frame B:
nprcvhknnnntkkslhcfllfffnf*ffi*fkqyinqlk*tltr*xkrkkkkkkkkkkk
kkkkxffxkkkxpppxkxfxggggxxxggxkxxkkkxfxgxkxfxkk---

---lq*vl*pqvlnvhi*wkevsh*ih*priwkcllnvsnvnielv*llrvnllatptql
isfihsskkkvniysmsvnlysviynkvvlllqlivsfqlv**iiiinswktisknmvif
k*mvvlvslmvvfitlqckk*skkcl

Frame C:
ipgvftkitttlkkvfivfyffflifnflfnlnni*in*NKXSLDXVKEKKKKKKKKKKK
KKKKXFXKKKXXPPPXXXFXGGGGXXXGGXKXXKKXFFXGKXXXXKK---

---cneffnlrc*tfiyngkryhieytnqgfgnvc*tfqt*t*nwsny*e*tc*qhllns
fhlftlqrrr*tfircp*iytrsfttrwysfcn*sylfnsfnesllsilgkrsqrtwsss
newlywfh*wwyslhsnarndrrnv*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHD874 (VHD874Q) /CSM/VH/VHD8-D/VHD874Q.Seq.d/                        854   0.0
VHP708 (VHP708Q) /CSM/VH/VHP7-A/VHP708Q.Seq.d/                        854   0.0
VHP472 (VHP472Q) /CSM/VH/VHP4-C/VHP472Q.Seq.d/                        854   0.0
VHP463 (VHP463Q) /CSM/VH/VHP4-C/VHP463Q.Seq.d/                        854   0.0
VHO736 (VHO736Q) /CSM/VH/VHO7-B/VHO736Q.Seq.d/                        854   0.0
VHO650 (VHO650Q) /CSM/VH/VHO6-C/VHO650Q.Seq.d/                        854   0.0
VHO434 (VHO434Q) /CSM/VH/VHO4-B/VHO434Q.Seq.d/                        854   0.0
VHO156 (VHO156Q) /CSM/VH/VHO1-C/VHO156Q.Seq.d/                        854   0.0
VHN894 (VHN894Q) /CSM/VH/VHN8-D/VHN894Q.Seq.d/                        854   0.0
VHN560 (VHN560Q) /CSM/VH/VHN5-C/VHN560Q.Seq.d/                        854   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   854   0.0    3
X94340|X94340.1 D.discoideum mRNA for 6-phosphofructo-1-kinase.   854   0.0    1
X89039|X89039.1 D.discoideum mRNA for 6-phosphofructo-1-kinase.   854   0.0    1
AJ430522|AJ430522.1 Dictyostelium discoideum partial pfk gene for ATP:D-fructose  6-phosphate 1-phosphofructokinase, exon 1.    88   2e-27  2
DX414156|DX414156.1 SOYC248TH LargeInsertSoybeanGenLibBuild4 Glycine max genomic clone H12A17:Build4MTP4H23, genomic survey sequence.    48   0.30   1
CE635221|CE635221.1 tigr-gss-dog-17000366743799 Dog Library Canis familiaris genomic, genomic survey sequence.    46   1.2    1
BZ298846|BZ298846.1 CG4616.f1 Candida glabrata Random Genomic Library Candida glabrata genomic clone CG4616, DNA sequence.    46   1.2    1
BZ297417|BZ297417.1 CG3420.r1 Candida glabrata Random Genomic Library Candida glabrata genomic clone CG3420, DNA sequence.    46   1.2    1
CR380955|CR380955.1 Candida glabrata strain CBS138 chromosome I complete sequence.    46   1.2    1
U97104|U97104.1 Bactrocera tryoni membrane transporter (white) gene, complete cds.    46   1.2    1
dna update 2006. 3.31
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P90521) RecName: Full=6-phosphofructokinase;          Short=Pho...   290   2e-77
CP001337_2617(CP001337|pid:none) Chloroflexus aggregans DSM 9485...   117   4e-25
BC081652_1(BC081652|pid:none) Danio rerio phosphofructokinase, m...   103   6e-21
(Q867C9) RecName: Full=6-phosphofructokinase, muscle type;      ...    99   1e-19
DQ480147_1(DQ480147|pid:none) Sus scrofa muscle 6-phosphofructok...    98   3e-19
BC077756_1(BC077756|pid:none) Xenopus laevis MGC79063 protein, m...    98   3e-19
AY335614_1(AY335614|pid:none) Synthetic construct Homo sapiens p...    97   5e-19
CR858939_1(CR858939|pid:none) Pongo abelii mRNA; cDNA DKFZp468O1...    97   5e-19
(P08237) RecName: Full=6-phosphofructokinase, muscle type;      ...    97   5e-19
(Q0IIG5) RecName: Full=6-phosphofructokinase, muscle type;      ...    97   5e-19
protein update 2009. 4.19
PSORT 

 psg: 0.03  gvh: 0.41  alm: 0.54  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.74  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 76.0 %: nuclear
	 16.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: cytoplasmic

>> prediction for SHG495 is nuc
5' end seq. ID SHG495F
5' end seq. 
>SHG495F.Seq
AAATCCCAGGTGTGTTCACAAAAATAACAACAACACTAAAAAAAGTCTTCATTGTTTTTT
ACTTTTTTTTTTTAATTTTTAATTTTTTATTTAATTTAAACAATATATAAATCAATTAAA
ATAAACNCTCACTCGATAGNGTAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAANTTTTTTNAAAAAAAAAAANGNCCCCCCCCCCNAAAANNTTTTTN
NGGGGGGGGGGGGGNNAANTTNGGGGGGNNAAAAAANNNNAAAAAAAAANTTTTTTNGGG
GNAAAAANNTTTTNNAAAAAAAANNNNNNNNNN
Length of 5' end seq. 333
3' end seq. ID SHG495Z
3' end seq. 
>SHG495Z.Seq
NNNNNNNNNNGCTGCAATGAGTTCTTTAACCTCAGGTGCTGAACGTTCATATATAATGGA
AAGAGGTATCACATTGAATACACTAACCAAGGATTTGGAAATGTTTGTTGAACGTTTCAA
ACGTGAACATAGAATTGGTCTAATTATTAAGAGTGAACTTGCTAGCAACACCTACTCAAC
TCATTTCATTTATTCACTCTTCAAAGAAGAAGGTAAACATTTATTCGATGTCCGTGAATC
TATACTCGGTCATTTACAACAAGGTGGTACTCCTTCTGCAATTGATCGTATCTTTTCAAC
TCGTTTAATGAATCATTATTATCAATTCTTGGAAAACGATCTCAAAGAACATGGTCATCT
TCAAATGAATGGTTGTATTGGTTTCATTGATGGTGGTATTCATTACACTCCAATGCAAGA
AATGATCGAAGAAATGTCTGA
Length of 3' end seq. 441
Connected seq. ID SHG495P
Connected seq. 
>SHG495P.Seq
AAATCCCAGGTGTGTTCACAAAAATAACAACAACACTAAAAAAAGTCTTCATTGTTTTTT
ACTTTTTTTTTTTAATTTTTAATTTTTTATTTAATTTAAACAATATATAAATCAATTAAA
ATAAACNCTCACTCGATAGNGTAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAANTTTTTTNAAAAAAAAAAANGNCCCCCCCCCCNAAAANNTTTTTN
NGGGGGGGGGGGGGNNAANTTNGGGGGGNNAAAAAANNNNAAAAAAAAANTTTTTTNGGG
GNAAAAANNTTTTNNAAAAAAAA----------GCTGCAATGAGTTCTTTAACCTCAGGT
GCTGAACGTTCATATATAATGGAAAGAGGTATCACATTGAATACACTAACCAAGGATTTG
GAAATGTTTGTTGAACGTTTCAAACGTGAACATAGAATTGGTCTAATTATTAAGAGTGAA
CTTGCTAGCAACACCTACTCAACTCATTTCATTTATTCACTCTTCAAAGAAGAAGGTAAA
CATTTATTCGATGTCCGTGAATCTATACTCGGTCATTTACAACAAGGTGGTACTCCTTCT
GCAATTGATCGTATCTTTTCAACTCGTTTAATGAATCATTATTATCAATTCTTGGAAAAC
GATCTCAAAGAACATGGTCATCTTCAAATGAATGGTTGTATTGGTTTCATTGATGGTGGT
ATTCATTACACTCCAATGCAAGAAATGATCGAAGAAATGTCTGA
Length of connected seq. 754
Full length Seq ID -
Full length Seq. -
Length of full length seq. -