>SHG345 (SHG345Q) /CSM/SH/SHG3-B/SHG345Q.Seq.d/
AATAAAAAACGAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGGTC
AAAATGAAGAATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGAGC
AACAATTAACTAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGATAG
AAGAAGCATTAGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTCAA
TTTATAATAAACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCATAAAA
TTTTCAATAAAGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTAAAAG
AAACAGCAGAAAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGAAT
CATCAGAAGGTGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTTAT
CAGCATTTGTAAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATCGA
TCTATCAATCACAAAAGAATATACATACACAATTCTTTAAGAATGTTCATTTACAAGCTG
TCTATTATTCAATGATTGATATXXXXXXXXXXTTGGTGACAGAGTTGGAAAGAGACATTA
GACAATTGGGTGTCTCCGAGACTGGTTTAACCTTTTTAGATCATTTTAGACTTTTAATCA
CTCATATTGGTAATGCTATGGGTTATATTAGATTGGTCAGATCCGGTGGTTTACACTATT
GTAGTAATGCTATCAAATTCGTACCAGACTTGAAAAAGATTCCAAAATTCCAAGATTTAA
CCTCAAAAGATGCCCTCTCACCTGAAACAATTCAAGCTAGTACCAATTTGGATTCAATCA
TTCATAATCTTTCAAATAATTTATCAGAGGGTACTGAATACTTTAAGATGTTGGTAAACG
TTTTCGCTACAGAGTTTCGAAATATCGCTAATCAACATTTAAAGAATTTCTATATCATCG
TACCTGCCTTGGCCTCAAATTATATCGATCATATGATCAATAGTAAAGATAAACTCTTTA
AGAAATCAAAAGCAATCGGTGCTGAAGCTTTATTATTCACTGATGATGGCTTTGCAATTG
GTTTGGCTTATATCTTAAAATTATTAGATCAAAATAAAGATTTCGATTCTCTTCATTGGT
TCGATCGTATTCAATTGAAATGTGAAACTGATCAAAAGAGAATGTTGGCTGAAGCAAATG
CAAAAGGTATCAAAGGGGATCAAAATCATTTAGCAATTAAAAAGATTCAAAATTTCCAAC
AAGAATA

*ktnnpi*KMATSYSANNGQNEEYTFDAFEDGSSKLVGEQQLTKLNQFAVDYAQQIWKIE
EALDETLNEVWDINIDPVSIYNKPHEQLDLIELVKTDHKIFNKVILVFSSICNQTRILKE
TAESKFYSPLTVFGEITGESSEGDVQIEVGKLLPFMIDLSAFVNRCYSLIRNIISQFASI
YQSQKNIHTQFFKNVHLQAVYYSMID---

---LVTELERDIRQLGVSETGLTFLDHFRLLITHIGNAMGYIRLVRSGGLHYCSNAIKFV
PDLKKIPKFQDLTSKDALSPETIQASTNLDSIIHNLSNNLSEGTEYFKMLVNVFATEFRN
IANQHLKNFYIIVPALASNYIDHMINSKDKLFKKSKAIGAEALLFTDDGFAIGLAYILKL
LDQNKDFDSLHWFDRIQLKCETDQKRMLAEANAKGIKGDQNHLAIKKIQNFQQE

Frame A:
nkkrttqfrkwphpirlimvkmknihlmhlkmvhlnwlesnn*lnlinlqlimhnkfgr*
kkh*mkh*mkygi*i*iqfqfiinlmnn*i*lnwlkliikfsik*y*yslqfviklef*k
kqqkvnfihh*qylerlqvnhqkvmyklklvnyyhl*liyqhl*idvih*leillvnlhr
sinhkriyihnslrmfiyklsiiq*li---

---LVTELERDIRQLGVSETGLTFLDHFRLLITHIGNAMGYIRLVRSGGLHYCSNAIKFV
PDLKKIPKFQDLTSKDALSPETIQASTNLDSIIHNLSNNLSEGTEYFKMLVNVFATEFRN
IANQHLKNFYIIVPALASNYIDHMINSKDKLFKKSKAIGAEALLFTDDGFAIGLAYILKL
LDQNKDFDSLHWFDRIQLKCETDQKRMLAEANAKGIKGDQNHLAIKKIQNFQQE

Frame B:
ikneqpnlenghilfg**wsk*riyi*ci*rwfi*igwratin*t*sics*lcttnledr
rsir*nik*smgykyrssfnl**ts*tirfn*ig*n*s*nfq*snisilfnl*sn*nfkr
nsrk*ilftinsiwrdyr*iirr*ctn*sw*iitiyd*fisick*mlfin*kyy*sicid
lsitkeytytil*ecsftscllfnd*y---

---w*qswketldnwvsprlv*pf*iildf*slilvmlwvildwsdpvvytivvmlsnsy
qt*krfqnski*pqkmpshlkqfklvpiwiqsfiifqiiyqrvlntlrcw*tfslqsfei
slini*risissylpwpqiisii*sivkinslrnqkqsvlklyyslmmalqlvwlis*ny
*ikikisilfigsivfn*nvklikrecwlkqmqkvskgikii*qlkrfkisnkn

Frame C:
*ktnnpi*KMATSYSANNGQNEEYTFDAFEDGSSKLVGEQQLTKLNQFAVDYAQQIWKIE
EALDETLNEVWDINIDPVSIYNKPHEQLDLIELVKTDHKIFNKVILVFSSICNQTRILKE
TAESKFYSPLTVFGEITGESSEGDVQIEVGKLLPFMIDLSAFVNRCYSLIRNIISQFASI
YQSQKNIHTQFFKNVHLQAVYYSMID---

---gdrvgkrh*tigclrdwfnlfrsf*tfnhsyw*cygly*igqirwftll**cyqirt
rlekdskiprfnlkrcplt*nnss*yqfgfnhs*sfk*firgy*il*dvgkrfryrvsky
r*stfkeflyhrtclglklyrsydq**r*tl*eiksnrc*sfiih**wlcnwfglylkii
rsk*rfrfsslvrsysiem*n*skenvg*skckryqrgsksfsn*kdskfptri

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHG345 (SHG345Q) /CSM/SH/SHG3-B/SHG345Q.Seq.d/                       2571   0.0
VHP871 (VHP871Q) /CSM/VH/VHP8-C/VHP871Q.Seq.d/                       1362   0.0
VHK538 (VHK538Q) /CSM/VH/VHK5-B/VHK538Q.Seq.d/                       1362   0.0
VHK424 (VHK424Q) /CSM/VH/VHK4-A/VHK424Q.Seq.d/                       1362   0.0
VHI658 (VHI658Q) /CSM/VH/VHI6-C/VHI658Q.Seq.d/                       1362   0.0
VHI518 (VHI518Q) /CSM/VH/VHI5-A/VHI518Q.Seq.d/                       1362   0.0
VHB863 (VHB863Q) /CSM/VH/VHB8-C/VHB863Q.Seq.d/                       1362   0.0
VHB821 (VHB821Q) /CSM/VH/VHB8-A/VHB821Q.Seq.d/                       1362   0.0
VHB614 (VHB614Q) /CSM/VH/VHB6-A/VHB614Q.Seq.d/                       1362   0.0
VHB189 (VHB189Q) /CSM/VH/VHB1-D/VHB189Q.Seq.d/                       1362   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BU746157|BU746157.1 CH3#002_C07T7 Canine heart normalized cDNA Library in pBluescriptCanis familiaris cDNA clone CH3#002_C07 5', mRNA sequence.    38   7e-04  3
BY464171|BY464171.1 Mus musculus 15 days pregnant adult female amnion cDNA, RIKEN full-length enriched library, clone:K630150M24, 3' end partial sequence.    42   0.027  2
AK113047|AK113047.1 Ciona intestinalis cDNA, clone:ciad002a21, full insert sequence.    48   0.030  2
AC168241|AC168241.2 Bos taurus clone CH240-197K22, *** SEQUENCING IN PROGRESS ***, 28 unordered pieces.    52   0.033  1
BB822673|BB822673.1 Mus musculus mammary gland RCB-0526 Jyg-MC(A) cDNA, RIKEN full-length enriched library, clone:G830022O04, 3' end partial sequence.    32   0.099  4
DN287505|DN287505.1 1241755 MARC 7BOV Bos taurus cDNA 3', mRNA sequence.    34   0.12   3
CR103667|CR103667.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP142o16.    50   0.13   1
CR044130|CR044130.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP186g09.    50   0.13   1
CR034434|CR034434.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP47e19.    50   0.13   1
CR023465|CR023465.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP421e10.    50   0.13   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54R74) RecName: Full=UPF0681 protein;                               457   e-127
BC040936_1(BC040936|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   228   4e-58
AB028956_1(AB028956|pid:none) Homo sapiens mRNA for KIAA1033 pro...   228   5e-58
BC143373_1(BC143373|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   228   5e-58
BC020314_1(BC020314|pid:none) Mus musculus RIKEN cDNA A230046K03...   226   2e-57
(Q3UMB9) RecName: Full=UPF0681 protein KIAA1033;                      226   2e-57
AK161247_1(AK161247|pid:none) Mus musculus adult male testis cDN...   226   2e-57
AK145000_1(AK145000|pid:none) Mus musculus mammary gland RCB-052...   226   2e-57
CP000600_221(CP000600|pid:none) Ostreococcus lucimarinus CCE9901...   174   5e-42
BC110850_1(BC110850|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   174   6e-42

 psg: 0.79  gvh: 0.25  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 40.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for SHG345 is nuc

>SHG345F.Seq
AATAAAAAACGAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGGTC
AAAATGAAGAATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGAGC
AACAATTAACTAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGATAG
AAGAAGCATTAGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTCAA
TTTATAATAAACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCATAAAA
TTTTCAATAAAGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTAAAAG
AAACAGCAGAAAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGAAT
CATCAGAAGGTGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTTAT
CAGCATTTGTAAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATCGA
TCTATCAATCACAAAAGAATATACATACACAATTCTTTAAGAATGTTCATTTACAAGCTG
TCTATTATTCAATGATTGATATNNNNNNNNNN

>SHG345Z.Seq
NNNNNNNNNNTTGGTGACAGAGTTGGAAAGAGACATTAGACAATTGGGTGTCTCCGAGAC
TGGTTTAACCTTTTTAGATCATTTTAGACTTTTAATCACTCATATTGGTAATGCTATGGG
TTATATTAGATTGGTCAGATCCGGTGGTTTACACTATTGTAGTAATGCTATCAAATTCGT
ACCAGACTTGAAAAAGATTCCAAAATTCCAAGATTTAACCTCAAAAGATGCCCTCTCACC
TGAAACAATTCAAGCTAGTACCAATTTGGATTCAATCATTCATAATCTTTCAAATAATTT
ATCAGAGGGTACTGAATACTTTAAGATGTTGGTAAACGTTTTCGCTACAGAGTTTCGAAA
TATCGCTAATCAACATTTAAAGAATTTCTATATCATCGTACCTGCCTTGGCCTCAAATTA
TATCGATCATATGATCAATAGTAAAGATAAACTCTTTAAGAAATCAAAAGCAATCGGTGC
TGAAGCTTTATTATTCACTGATGATGGCTTTGCAATTGGTTTGGCTTATATCTTAAAATT
ATTAGATCAAAATAAAGATTTCGATTCTCTTCATTGGTTCGATCGTATTCAATTGAAATG
TGAAACTGATCAAAAGAGAATGTTGGCTGAAGCAAATGCAAAAGGTATCAAAGGGGATCA
AAATCATTTAGCAATTAAAAAGATTCAAAATTTCCAACAAGAATA

>SHG345P.Seq
AATAAAAAACGAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGGTC
AAAATGAAGAATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGAGC
AACAATTAACTAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGATAG
AAGAAGCATTAGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTCAA
TTTATAATAAACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCATAAAA
TTTTCAATAAAGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTAAAAG
AAACAGCAGAAAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGAAT
CATCAGAAGGTGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTTAT
CAGCATTTGTAAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATCGA
TCTATCAATCACAAAAGAATATACATACACAATTCTTTAAGAATGTTCATTTACAAGCTG
TCTATTATTCAATGATTGATAT----------TTGGTGACAGAGTTGGAAAGAGACATTA
GACAATTGGGTGTCTCCGAGACTGGTTTAACCTTTTTAGATCATTTTAGACTTTTAATCA
CTCATATTGGTAATGCTATGGGTTATATTAGATTGGTCAGATCCGGTGGTTTACACTATT
GTAGTAATGCTATCAAATTCGTACCAGACTTGAAAAAGATTCCAAAATTCCAAGATTTAA
CCTCAAAAGATGCCCTCTCACCTGAAACAATTCAAGCTAGTACCAATTTGGATTCAATCA
TTCATAATCTTTCAAATAATTTATCAGAGGGTACTGAATACTTTAAGATGTTGGTAAACG
TTTTCGCTACAGAGTTTCGAAATATCGCTAATCAACATTTAAAGAATTTCTATATCATCG
TACCTGCCTTGGCCTCAAATTATATCGATCATATGATCAATAGTAAAGATAAACTCTTTA
AGAAATCAAAAGCAATCGGTGCTGAAGCTTTATTATTCACTGATGATGGCTTTGCAATTG
GTTTGGCTTATATCTTAAAATTATTAGATCAAAATAAAGATTTCGATTCTCTTCATTGGT
TCGATCGTATTCAATTGAAATGTGAAACTGATCAAAAGAGAATGTTGGCTGAAGCAAATG
CAAAAGGTATCAAAGGGGATCAAAATCATTTAGCAATTAAAAAGATTCAAAATTTCCAAC
AAGAATA