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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHG302
Library SH
(Link to library)
Clone ID SHG302
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10794-1
Original site URL
Representative seq. ID SHG302P
(Link to Original site)
Representative DNA sequence 
>SHG302 (SHG302Q) /CSM/SH/SHG3-A/SHG302Q.Seq.d/
GATATCAATAATAAATATTATTACATATACAAAACAAAATTAATAAAACTTAAACTTTAT
TTTTCCCCTACATTGGCCAAACTTTAATTTGCCTTTGTACAATTATTCTCTTTAAACTCT
TATTATTAAAATTCATTTTAAAAACTCTCATTTCTAAAAAAACTAAAACAAAAAAAAAAN
GGTCAATTTTACAATANATCAAATTCGTTCAATTATGGATCGTCGNGAAAATATCAAAAA
TATGTCTGTTATNGCTCATGTTGATCATGGTAAAACTACTCTTACTGATTCATTAATTCA
AANAGCTGGTNTTATTGCAAATAAGGTATCTGGTAATGCAGGATATATGTCATGTCGTCC
AAATNAACAATTACGTGGTATTACCATAAAGTCATCATCTGTTTCACTTCACTTTGAAAT
GCCAAAAGAXXXXXXXXXXCTACATGAAATCTGAACCATTATCGTCAGACCTAACTGATG
ATATTGATAATGGTTTGGTGGCTTTTAATCAAGATGTTAAAAGTCGTGCCAATTATCTCT
ATGATAAACATTCTTGGGATAAGAATGATGCAGTTAACATTTGGTCATTTGGTCCAGAAT
ATAAGGGTCCAAATGTTTTAGTTAACAAAACCAAAGGTGTTCAATATCTTCATGAAATTA
AAGACTCTATGGTTTCAGCATTCCAATGGATCTCAAATGAAGGTGTATTATGTGGTGAGA
AAATGAGATCGATTCGTTTCAACGTTTATGATGTAATGCTTCATGCCGATGCAATTCATA
GAGGTAGTGGTCAATTTATTCCAACAACTCGTCGTGTAATGTATGCCTCCCAACTATCAG
CTCAACCAACACTTTTGGAACCAATGTATTTGGTTGATATTAGTACTCCTGAAAGTGGTA
TTAGTGGTATCTACTCTGTACTCAATCGTAGAAGAGGTGTTGTCATTGGTGAAGAACGTC
GTATTGGTACTCCATTATTCAATATCAAAGCTTATCTTCCTGTAATGGAATCATTTGGTC
TCACTGCTGATTTACGTTCAAGTACTGCTGATCAAGCTTTCCCTCAATGTGTTTTTGATC
ATTGGAGTTCAATTGGTATCGTAGGACAAAAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
isiiniitytkqn**nlnfifplhwpnfnlplynysl*tliikihfknshf*kn*NKKKX
VNFTIXQIRSIMDRRENIKNMSVXAHVDHGKTTLTDSLIQXAGXIANKVSGNAGYMSCRP
NXQLRGITIKSSSVSLHFEMPK---

---YMKSEPLSSDLTDDIDNGLVAFNQDVKSRANYLYDKHSWDKNDAVNIWSFGPEYKGP
NVLVNKTKGVQYLHEIKDSMVSAFQWISNEGVLCGEKMRSIRFNVYDVMLHADAIHRGSG
QFIPTTRRVMYASQLSAQPTLLEPMYLVDISTPESGISGIYSVLNRRRGVVIGEERRIGT
PLFNIKAYLPVMESFGLTADLRSSTADQAFPQCVFDHWSSIGIVGQKK
Translated Amino Acid sequence (All Frames) 
Frame A:
dinnkyyyiyktkliklklyfsptlakl*fafvqlfslnsyy*nsf*klsflkklkqkkx
gqfynxsnsfnygssxkyqkyvcyxsc*sw*nysy*finsxswxyck*giw*criyvmss
kxtitwyyhkviicftsl*nakr---

---lhei*tiivrpn**y**wfggf*src*kscqlsl**tflg*e*cs*hlviwsri*gs
kcfs*qnqrcsiss*n*rlygfsipmdlk*rcimw*eneidsfqrl*cnascrcns*r*w
siysnnsscnvclptisstntfgtnvfg*y*ys*kwy*wyllctqs*krcchw*rtsywy
siiqyqslsscngiiwshc*ftfkyc*ssfpsmcf*slefnwyrrtkk

Frame B:
isiiniitytkqn**nlnfifplhwpnfnlplynysl*tliikihfknshf*kn*NKKKX
VNFTIXQIRSIMDRRENIKNMSVXAHVDHGKTTLTDSLIQXAGXIANKVSGNAGYMSCRP
NXQLRGITIKSSSVSLHFEMPK---

---YMKSEPLSSDLTDDIDNGLVAFNQDVKSRANYLYDKHSWDKNDAVNIWSFGPEYKGP
NVLVNKTKGVQYLHEIKDSMVSAFQWISNEGVLCGEKMRSIRFNVYDVMLHADAIHRGSG
QFIPTTRRVMYASQLSAQPTLLEPMYLVDISTPESGISGIYSVLNRRRGVVIGEERRIGT
PLFNIKAYLPVMESFGLTADLRSSTADQAFPQCVFDHWSSIGIVGQKK

Frame C:
yq**illhiqnkinkt*tlffpyigqtliclctiilfkllllkfilktliskktktkkkx
silqxikfvqlwivxkiskicllxlmlimvkllllih*fkxlvllqirylvmqdichvvq
xnnyvvlp*shhlfhftlkcqk---

---t*nlnhyrqt*lmilimvwwllikmlkvvpiisminilgirmmqltfghlvqnirvq
mf*ltkpkvfnifmklktlwfqhsngsqmkvyyvvrk*drfvstfmm*cfmpmqfievvv
nlfqqlvv*cmppnyqlnqhfwnqciwlilvllkvvlvvstlysiveevlslvknvvlvl
hysisklifl*wnhlvslliyvqvlliklslnvfliigvqlvs*dkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHG302 (SHG302Q) /CSM/SH/SHG3-A/SHG302Q.Seq.d/                       1742   0.0
AHN643 (AHN643Q) /CSM/AH/AHN6-B/AHN643Q.Seq.d/                       1713   0.0
AHM892 (AHM892Q) /CSM/AH/AHM8-D/AHM892Q.Seq.d/                       1707   0.0
VHJ795 (VHJ795Q) /CSM/VH/VHJ7-D/VHJ795Q.Seq.d/                       1693   0.0
VHO117 (VHO117Q) /CSM/VH/VHO1-A/VHO117Q.Seq.d/                       1306   0.0
VHN538 (VHN538Q) /CSM/VH/VHN5-B/VHN538Q.Seq.d/                       1306   0.0
VHK348 (VHK348Q) /CSM/VH/VHK3-B/VHK348Q.Seq.d/                       1306   0.0
VHB815 (VHB815Q) /CSM/VH/VHB8-A/VHB815Q.Seq.d/                       1306   0.0
VHB107 (VHB107Q) /CSM/VH/VHB1-A/VHB107Q.Seq.d/                       1306   0.0
SHG365 (SHG365Q) /CSM/SH/SHG3-C/SHG365Q.Seq.d/                       1306   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.   117   9e-78  9
X15386|X15386.1 Dictyostelium discoideum H6 gene mRNA.            276   3e-74  2
BI863575|BI863575.1 kx45h07.y1 Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to SW:EF2_CHICK Q90705 ELONGATION FACTOR 2 ;, mRNA sequence.    66   3e-18  3
BI451383|BI451383.1 kx23e09.y1 Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to SW:EF2_CAEEL P29691 ELONGATION FACTOR 2 ;, mRNA sequence.    66   3e-18  3
BI863585|BI863585.1 kx46a06.y1 Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to SW:EF2_CAEEL P29691 ELONGATION FACTOR 2 ;, mRNA sequence.    66   3e-18  3
BM056853|BM056853.1 2154-85 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides felis cDNA clone 2154-85, mRNA sequence.    84   8e-12  1
DR447725|DR447725.1 AR1047E10 A. gomesiana hemocytes normalized library Acanthoscurria gomesiana cDNA clone AR1047E10 5', mRNA sequence.    80   1e-11  2
DR443579|DR443579.1 AR2018A03 A. gomesiana hemocytes library Acanthoscurria gomesiana cDNA clone AR2018A03 5', mRNA sequence.    80   1e-11  2
BM058578|BM058578.1 2193-94 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides felis cDNA clone 2193-94, mRNA sequence.    82   3e-11  1
BM058379|BM058379.1 2191-29 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides felis cDNA clone 2191-29, mRNA sequence.    82   3e-11  1
dna update 2006. 3.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   272   1e-71
AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   268   2e-70
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   267   4e-70
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   267   5e-70
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   267   5e-70
AK032997_1(AK032997|pid:none) Mus musculus 12 days embryo male w...   267   5e-70
(P05197) RecName: Full=Elongation factor 2;          Short=EF-2;...   267   5e-70
AJ278147_1(AJ278147|pid:none) Rattus norvegicus partial Ef-2 gen...   267   5e-70
AK159514_1(AK159514|pid:none) Mus musculus osteoclast-like cell ...   267   5e-70
M76131_1(M76131|pid:none) Mouse elongation factor 2 (ef-2) mRNA,...   267   5e-70
protein update 2009. 4.18
PSORT 

 psg: 0.80  gvh: 0.47  alm: 0.48  top: 0.53  tms: 0.00  mit: 0.33  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 28.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for SHG302 is nuc
5' end seq. ID SHG302F
5' end seq. 
>SHG302F.Seq
GATATCAATAATAAATATTATTACATATACAAAACAAAATTAATAAAACTTAAACTTTAT
TTTTCCCCTACATTGGCCAAACTTTAATTTGCCTTTGTACAATTATTCTCTTTAAACTCT
TATTATTAAAATTCATTTTAAAAACTCTCATTTCTAAAAAAACTAAAACAAAAAAAAAAN
GGTCAATTTTACAATANATCAAATTCGTTCAATTATGGATCGTCGNGAAAATATCAAAAA
TATGTCTGTTATNGCTCATGTTGATCATGGTAAAACTACTCTTACTGATTCATTAATTCA
AANAGCTGGTNTTATTGCAAATAAGGTATCTGGTAATGCAGGATATATGTCATGTCGTCC
AAATNAACAATTACGTGGTATTACCATAAAGTCATCATCTGTTTCACTTCACTTTGAAAT
GCCAAAAGANNNNNNNNNN
Length of 5' end seq. 439
3' end seq. ID SHG302Z
3' end seq. 
>SHG302Z.Seq
NNNNNNNNNNCTACATGAAATCTGAACCATTATCGTCAGACCTAACTGATGATATTGATA
ATGGTTTGGTGGCTTTTAATCAAGATGTTAAAAGTCGTGCCAATTATCTCTATGATAAAC
ATTCTTGGGATAAGAATGATGCAGTTAACATTTGGTCATTTGGTCCAGAATATAAGGGTC
CAAATGTTTTAGTTAACAAAACCAAAGGTGTTCAATATCTTCATGAAATTAAAGACTCTA
TGGTTTCAGCATTCCAATGGATCTCAAATGAAGGTGTATTATGTGGTGAGAAAATGAGAT
CGATTCGTTTCAACGTTTATGATGTAATGCTTCATGCCGATGCAATTCATAGAGGTAGTG
GTCAATTTATTCCAACAACTCGTCGTGTAATGTATGCCTCCCAACTATCAGCTCAACCAA
CACTTTTGGAACCAATGTATTTGGTTGATATTAGTACTCCTGAAAGTGGTATTAGTGGTA
TCTACTCTGTACTCAATCGTAGAAGAGGTGTTGTCATTGGTGAAGAACGTCGTATTGGTA
CTCCATTATTCAATATCAAAGCTTATCTTCCTGTAATGGAATCATTTGGTCTCACTGCTG
ATTTACGTTCAAGTACTGCTGATCAAGCTTTCCCTCAATGTGTTTTTGATCATTGGAGTT
CAATTGGTATCGTAGGACAAAAAAAA
Length of 3' end seq. 686
Connected seq. ID SHG302P
Connected seq. 
>SHG302P.Seq
GATATCAATAATAAATATTATTACATATACAAAACAAAATTAATAAAACTTAAACTTTAT
TTTTCCCCTACATTGGCCAAACTTTAATTTGCCTTTGTACAATTATTCTCTTTAAACTCT
TATTATTAAAATTCATTTTAAAAACTCTCATTTCTAAAAAAACTAAAACAAAAAAAAAAN
GGTCAATTTTACAATANATCAAATTCGTTCAATTATGGATCGTCGNGAAAATATCAAAAA
TATGTCTGTTATNGCTCATGTTGATCATGGTAAAACTACTCTTACTGATTCATTAATTCA
AANAGCTGGTNTTATTGCAAATAAGGTATCTGGTAATGCAGGATATATGTCATGTCGTCC
AAATNAACAATTACGTGGTATTACCATAAAGTCATCATCTGTTTCACTTCACTTTGAAAT
GCCAAAAGA----------CTACATGAAATCTGAACCATTATCGTCAGACCTAACTGATG
ATATTGATAATGGTTTGGTGGCTTTTAATCAAGATGTTAAAAGTCGTGCCAATTATCTCT
ATGATAAACATTCTTGGGATAAGAATGATGCAGTTAACATTTGGTCATTTGGTCCAGAAT
ATAAGGGTCCAAATGTTTTAGTTAACAAAACCAAAGGTGTTCAATATCTTCATGAAATTA
AAGACTCTATGGTTTCAGCATTCCAATGGATCTCAAATGAAGGTGTATTATGTGGTGAGA
AAATGAGATCGATTCGTTTCAACGTTTATGATGTAATGCTTCATGCCGATGCAATTCATA
GAGGTAGTGGTCAATTTATTCCAACAACTCGTCGTGTAATGTATGCCTCCCAACTATCAG
CTCAACCAACACTTTTGGAACCAATGTATTTGGTTGATATTAGTACTCCTGAAAGTGGTA
TTAGTGGTATCTACTCTGTACTCAATCGTAGAAGAGGTGTTGTCATTGGTGAAGAACGTC
GTATTGGTACTCCATTATTCAATATCAAAGCTTATCTTCCTGTAATGGAATCATTTGGTC
TCACTGCTGATTTACGTTCAAGTACTGCTGATCAAGCTTTCCCTCAATGTGTTTTTGATC
ATTGGAGTTCAATTGGTATCGTAGGACAAAAAAAA
Length of connected seq. 1105
Full length Seq ID -
Full length Seq. -
Length of full length seq. -