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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHG248
Library SH
(Link to library)
Clone ID SHG248
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15709-1
Original site URL
Representative seq. ID SHG248P
(Link to Original site)
Representative DNA sequence 
>SHG248 (SHG248Q) /CSM/SH/SHG2-B/SHG248Q.Seq.d/
ATAAAAATAATTCTAAACTATAAAATAACAATTATAAAAAAACAATAACAATAATAATAA
TAATTAATATATAGAAAAAATTATCAACAAGATCAGATAGAGTTAAATCTGTTGATATTC
ACCCAACAGAACCATGGATTTTAGCAAGTTTATATGATGGTAATGTTTATATTTGGAATT
ATGAAACTCAAAATATGGTAAAATCATTTGAAGTTTCACCAAATAATCCAGTTAGAACAG
CAAGATTCATTGCAAAGAAACAATGGATAGTTACAGGTAGTGATGATACATATATTAGAG
TTTATAATTATAATACAATGGAAAAGATAAAATCATTTGNAGCACATGCCGATTATATTA
GATGTATTATAGTTCATCCAACATTACCATATATATTATCATCATCGGATGATATGTTTA
TAAAGTTATGGGATTATGAAAAGGGATGGAGTAACACTCAAGTCTTTGAAGGACATTCAC
ATTACGTCATGTCGATTGCATGGAATCCAAAGGATACCAATCAATTTGCAACCGCATCAT
TGGATAAAACTGTGAAAGTGTGGAGTATAAACTCACCACATCCTCACTTTACATTGGAGG
GACACGAAAAGGGTATCAACAGTGTCGAGTATTTCTCTGGCGGTGAAAAGCCATACTXXX
XXXXXXXAAGAGAGTTTAAAGAGTACAAGCTCAAAAGCATCCGATGCACTTTCAAATCCA
ATCGATTTCCCAAATTTATTCCCACAATATGATTTATCAATTAAAGCAGAGAAATATTTC
GAATCATTAAGATNTCAACCAAAATCATCTTTTGATTATTTAAAAAATGNTACTGCACAA
TCATTATCAAAGAAACAAAGAAACCTTTTAGAAGAGATTGAATCTGTTTCTCAACAAGAT
TATGATAAAGTTTTATCACAATTACAACATTTTAGTTTACATTCTGGTATTTCTGATATA
ACAACAACAACAACCAATACAAATAATAATACAAATAATACAAATAATAATAATACAGCA
TCACTTTTAGATGATCTAGATTTTACTTCAACCACCACCACTACTACAACCACTACAAAT
ACAAATACTACTCAAAAAGTTGCATCACCACTTTCATCACCATCACCAATTAGAAAGAAT
GATTCAATTGCTTCTCCACAAAAACAACAACAACAACAACAAGATATTAATATAACTGCA
CCACCACTCATTGATCCAAGTCCAGTTGATCATGATTTAGTTTTAGAACCAACTCCAATT
ATTAATACTGTATCACCAACTAAAACTTCTCCAACTGCCGATCTCTCATTTTTAGATAAT
ATACCAGTCGAT
sequence update 2002.10.25
Translated Amino Acid sequence 
knnskl*nnnykktitiiiiini*KKLSTRSDRVKSVDIHPTEPWILASLYDGNVYIWNY
ETQNMVKSFEVSPNNPVRTARFIAKKQWIVTGSDDTYIRVYNYNTMEKIKSFXAHADYIR
CIIVHPTLPYILSSSDDMFIKLWDYEKGWSNTQVFEGHSHYVMSIAWNPKDTNQFATASL
DKTVKVWSINSPHPHFTLEGHEKGINSVEYFSGGEKPY---

---ESLKSTSSKASDALSNPIDFPNLFPQYDLSIKAEKYFESLRXQPKSSFDYLKNXTAQ
SLSKKQRNLLEEIESVSQQDYDKVLSQLQHFSLHSGISDITTTTTNTNNNTNNTNNNNTA
SLLDDLDFTSTTTTTTTTTNTNTTQKVASPLSSPSPIRKNDSIASPQKQQQQQQDINITA
PPLIDPSPVDHDLVLEPTPIINTVSPTKTSPTADLSFLDNIPVD
Translated Amino Acid sequence (All Frames) 
Frame A:
ikiilnykitiikkq*q****liyrknyqqdqielnlliftqqnhgf*qvymmvmfifgi
mklkiw*nhlkfhqiiqleqqdslqrnng*lqvvmihilefiiiiqwkr*nhlxhmpiil
dvl*fiqhyhiyyhhrmicl*sygimkrdgvtlkslkdihitscrlhgiqripinlqphh
wikl*kcgv*thhiltlhwrdtkrvstvssislavksht---

---krv*rvqaqkhpmhfqiqsisqiyshnmiyqlkqrnisnh*dxnqnhllii*kmxlh
nhyqrnketf*krlnlflnkimikfyhnynilvyilvfli*qqqqpiqiiiqiiqiiiiq
hhf*mi*illqpppllqplqiqillkklhhhfhhhhqlermiqlllhknnnnnnkili*l
hhhsliqvqlimi*f*nqlqllilyhqlkllqlpishf*iiyqs

Frame B:
*k*f*tik*ql*knnnnnnnn*yiekiinkir*s*ic*yspnrtmdfskfi*w*clylel
*nskygkii*sftk*ss*nskihcketmdsyr***yiy*sl*l*yngkdkiixstcrly*
myysssnitiyiiiig*yvykvmgl*kgme*hssl*rtftlrhvdcmeskgyqsicnrii
g*ncesveyklttsslyiggtrkgyqqcrvflwr*kai---

---refkeyklksirctfksnrfpkfipti*fin*sreifriikxstkiif*lfkkxyct
iiiketkkpfrrd*icfstrl**sfitittf*ftfwyf*ynnnnnqyk**yk*yk***ys
itfr*srfyfnhhhyynhykykyyskscittfititn*ke*fncfstkttttttry*ync
ttth*skss*s*fsfrtnsny*ycitn*nfsncrslifr*ytsr

Frame C:
knnskl*nnnykktitiiiiini*KKLSTRSDRVKSVDIHPTEPWILASLYDGNVYIWNY
ETQNMVKSFEVSPNNPVRTARFIAKKQWIVTGSDDTYIRVYNYNTMEKIKSFXAHADYIR
CIIVHPTLPYILSSSDDMFIKLWDYEKGWSNTQVFEGHSHYVMSIAWNPKDTNQFATASL
DKTVKVWSINSPHPHFTLEGHEKGINSVEYFSGGEKPY---

---ESLKSTSSKASDALSNPIDFPNLFPQYDLSIKAEKYFESLRXQPKSSFDYLKNXTAQ
SLSKKQRNLLEEIESVSQQDYDKVLSQLQHFSLHSGISDITTTTTNTNNNTNNTNNNNTA
SLLDDLDFTSTTTTTTTTTNTNTTQKVASPLSSPSPIRKNDSIASPQKQQQQQQDINITA
PPLIDPSPVDHDLVLEPTPIINTVSPTKTSPTADLSFLDNIPVD
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHG248 (SHG248Q) /CSM/SH/SHG2-B/SHG248Q.Seq.d/                       1665   0.0
AHQ130 (AHQ130Q) /CSM/AH/AHQ1-B/AHQ130Q.Seq.d/                       1136   0.0
AHN840 (AHN840Q) /CSM/AH/AHN8-B/AHN840Q.Seq.d/                       1136   0.0
AHA122 (AHA122Q) /CSM/AH/AHA1-A/AHA122Q.Seq.d/                       1136   0.0
AHE547 (AHE547Q) /CSM/AH/AHE5-B/AHE547Q.Seq.d/                       1132   0.0
SHL384 (SHL384Q) /CSM/SH/SHL3-D/SHL384Q.Seq.d/                       1130   0.0
AHD201 (AHD201Q) /CSM/AH/AHD2-A/AHD201Q.Seq.d/                       1130   0.0
AHL191 (AHL191Q) /CSM/AH/AHL1-D/AHL191Q.Seq.d/                       1128   0.0
SHD239 (SHD239Q) /CSM/SH/SHD2-B/SHD239Q.Seq.d/                       1104   0.0
SHK472 (SHK472Q) /CSM/SH/SHK4-C/SHK472Q.Seq.d/                       1098   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AZ684437|AZ684437.1 ENTLH77TR Entamoeba histolytica Sheared DNA Entamoeba histolyticagenomic, genomic survey sequence.    66   4e-12  2
AP003487|AP003487.2 Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 6, BAC clone:OSJNBa0007O20.    46   7e-11  3
AK111584|AK111584.1 Oryza sativa (japonica cultivar-group) cDNA clone:J013089O14, full insert sequence.    46   8e-11  3
CL809452|CL809452.1 OR_CBa0024E02.f OR_CBa Oryza rufipogon genomic clone OR_CBa0024E02 5', genomic survey sequence.    46   8e-11  3
BP064336|BP064336.1 Lotus corniculatus var. japonicus cDNA, clone:GENLf038d07, 3' end.    68   1e-10  3
CN942611|CN942611.1 010921AVBC069045PG (AVBC) Royal Gala young shoot Malus x domestica cDNA clone AVBC069045, mRNA sequence.    78   2e-10  2
CN914665|CN914665.1 030110ABNB001773HT (ABNB) Braeburn cultured fruit cells, boron exposed, phytoremediation Malus x domestica cDNA clone ABNB001773, mRNA sequence.    78   2e-10  2
CN939139|CN939139.1 010616AVBC004822HT (AVBC) Royal Gala young shoot Malus x domestica cDNA clone AVBC004822, mRNA sequence.    78   3e-10  2
AY266451|AY266451.1 Plasmodium falciparum isolate 3D7 COPI protein (Sec27) mRNA, complete cds.    78   3e-10  3
AL929355|AL929355.1 Plasmodium falciparum strain 3D7, chromosome 9; segment 1/5.    78   6e-10  1
dna update 2006. 3.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54YD8) RecName: Full=Coatomer subunit beta'; AltName: Full=Bet...   407   e-112
(Q9C827) RecName: Full=Coatomer subunit beta'-2; AltName: Full=B...   310   9e-83
(Q6H8D5) RecName: Full=Coatomer subunit beta'-2; AltName: Full=B...   307   6e-82
AC011717_4(AC011717|pid:none) Arabidopsis thaliana chromosome 1 ...   307   6e-82
(Q9CAA0) RecName: Full=Coatomer subunit beta'-1; AltName: Full=B...   307   6e-82
AM438365_3(AM438365|pid:none) Vitis vinifera contig VV78X052512....   305   2e-81
BT036985_1(BT036985|pid:none) Zea mays full-length cDNA clone ZM...   302   2e-80
BC096500_1(BC096500|pid:none) Xenopus tropicalis hypothetical pr...   294   5e-78
AK304219_1(AK304219|pid:none) Homo sapiens cDNA FLJ61545 complet...   294   5e-78
(P35606) RecName: Full=Coatomer subunit beta'; AltName: Full=Bet...   294   5e-78
protein update 2009. 4.18
PSORT 

 psg: 0.62  gvh: 0.36  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.36  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: mitochondrial
	 32.0 %: cytoplasmic
	 20.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for SHG248 is mit
5' end seq. ID SHG248F
5' end seq. 
>SHG248F.Seq
ATAAAAATAATTCTAAACTATAAAATAACAATTATAAAAAAACAATAACAATAATAATAA
TAATTAATATATAGAAAAAATTATCAACAAGATCAGATAGAGTTAAATCTGTTGATATTC
ACCCAACAGAACCATGGATTTTAGCAAGTTTATATGATGGTAATGTTTATATTTGGAATT
ATGAAACTCAAAATATGGTAAAATCATTTGAAGTTTCACCAAATAATCCAGTTAGAACAG
CAAGATTCATTGCAAAGAAACAATGGATAGTTACAGGTAGTGATGATACATATATTAGAG
TTTATAATTATAATACAATGGAAAAGATAAAATCATTTGNAGCACATGCCGATTATATTA
GATGTATTATAGTTCATCCAACATTACCATATATATTATCATCATCGGATGATATGTTTA
TAAAGTTATGGGATTATGAAAAGGGATGGAGTAACACTCAAGTCTTTGAAGGACATTCAC
ATTACGTCATGTCGATTGCATGGAATCCAAAGGATACCAATCAATTTGCAACCGCATCAT
TGGATAAAACTGTGAAAGTGTGGAGTATAAACTCACCACATCCTCACTTTACATTGGAGG
GACACGAAAAGGGTATCAACAGTGTCGAGTATTTCTCTGGCGGTGAAAAGCCATACTNNN
NNNNNNN
Length of 5' end seq. 667
3' end seq. ID SHG248Z
3' end seq. 
>SHG248Z.Seq
NNNNNNNNNNAAGAGAGTTTAAAGAGTACAAGCTCAAAAGCATCCGATGCACTTTCAAAT
CCAATCGATTTCCCAAATTTATTCCCACAATATGATTTATCAATTAAAGCAGAGAAATAT
TTCGAATCATTAAGATNTCAACCAAAATCATCTTTTGATTATTTAAAAAATGNTACTGCA
CAATCATTATCAAAGAAACAAAGAAACCTTTTAGAAGAGATTGAATCTGTTTCTCAACAA
GATTATGATAAAGTTTTATCACAATTACAACATTTTAGTTTACATTCTGGTATTTCTGAT
ATAACAACAACAACAACCAATACAAATAATAATACAAATAATACAAATAATAATAATACA
GCATCACTTTTAGATGATCTAGATTTTACTTCAACCACCACCACTACTACAACCACTACA
AATACAAATACTACTCAAAAAGTTGCATCACCACTTTCATCACCATCACCAATTAGAAAG
AATGATTCAATTGCTTCTCCACAAAAACAACAACAACAACAACAAGATATTAATATAACT
GCACCACCACTCATTGATCCAAGTCCAGTTGATCATGATTTAGTTTTAGAACCAACTCCA
ATTATTAATACTGTATCACCAACTAAAACTTCTCCAACTGCCGATCTCTCATTTTTAGAT
AATATACCAGTCGAT
Length of 3' end seq. 675
Connected seq. ID SHG248P
Connected seq. 
>SHG248P.Seq
ATAAAAATAATTCTAAACTATAAAATAACAATTATAAAAAAACAATAACAATAATAATAA
TAATTAATATATAGAAAAAATTATCAACAAGATCAGATAGAGTTAAATCTGTTGATATTC
ACCCAACAGAACCATGGATTTTAGCAAGTTTATATGATGGTAATGTTTATATTTGGAATT
ATGAAACTCAAAATATGGTAAAATCATTTGAAGTTTCACCAAATAATCCAGTTAGAACAG
CAAGATTCATTGCAAAGAAACAATGGATAGTTACAGGTAGTGATGATACATATATTAGAG
TTTATAATTATAATACAATGGAAAAGATAAAATCATTTGNAGCACATGCCGATTATATTA
GATGTATTATAGTTCATCCAACATTACCATATATATTATCATCATCGGATGATATGTTTA
TAAAGTTATGGGATTATGAAAAGGGATGGAGTAACACTCAAGTCTTTGAAGGACATTCAC
ATTACGTCATGTCGATTGCATGGAATCCAAAGGATACCAATCAATTTGCAACCGCATCAT
TGGATAAAACTGTGAAAGTGTGGAGTATAAACTCACCACATCCTCACTTTACATTGGAGG
GACACGAAAAGGGTATCAACAGTGTCGAGTATTTCTCTGGCGGTGAAAAGCCATACT---
-------AAGAGAGTTTAAAGAGTACAAGCTCAAAAGCATCCGATGCACTTTCAAATCCA
ATCGATTTCCCAAATTTATTCCCACAATATGATTTATCAATTAAAGCAGAGAAATATTTC
GAATCATTAAGATNTCAACCAAAATCATCTTTTGATTATTTAAAAAATGNTACTGCACAA
TCATTATCAAAGAAACAAAGAAACCTTTTAGAAGAGATTGAATCTGTTTCTCAACAAGAT
TATGATAAAGTTTTATCACAATTACAACATTTTAGTTTACATTCTGGTATTTCTGATATA
ACAACAACAACAACCAATACAAATAATAATACAAATAATACAAATAATAATAATACAGCA
TCACTTTTAGATGATCTAGATTTTACTTCAACCACCACCACTACTACAACCACTACAAAT
ACAAATACTACTCAAAAAGTTGCATCACCACTTTCATCACCATCACCAATTAGAAAGAAT
GATTCAATTGCTTCTCCACAAAAACAACAACAACAACAACAAGATATTAATATAACTGCA
CCACCACTCATTGATCCAAGTCCAGTTGATCATGATTTAGTTTTAGAACCAACTCCAATT
ATTAATACTGTATCACCAACTAAAACTTCTCCAACTGCCGATCTCTCATTTTTAGATAAT
ATACCAGTCGAT
Length of connected seq. 1322
Full length Seq ID -
Full length Seq. -
Length of full length seq. -