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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHG225
Library SH
(Link to library)
Clone ID SHG225
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15718-1
Original site URL
Representative seq. ID SHG225P
(Link to Original site)
Representative DNA sequence 
>SHG225 (SHG225Q) /CSM/SH/SHG2-B/SHG225Q.Seq.d/
AATATTATAAATAAATAATGATGAAATATATATATATTTTATTAATATTTTCTTTATTGT
TTTTGAAAATAAATTCACAAAATACAATTTTGTTGAATGCATTAATTTATGATCAAACTC
CATCAAGAAATAATGATTTTCAAATTCCTTCTCCAGGAGGTATTGCAAAAGGGTTAGTAA
AATCTAATTTAGGTTCTGATAATACACCAGTTTTAGTGGCTGAGCGATCATCAACAATTC
ATTCACCAGAAACTTTTAATCAATGGTTTCACAATTATCCAGGAGTAAATTTACCAATTA
ATTATCAAATTACTTTAACTCAAAGTACTACAAATCCAAATGTTTATGCATATACAAATG
ATGCCTTTTTTCCAATAGATGGACAAGGATTTGATAATAAGACAAATTATCCATATGAAG
CCGTATATAGAGATGAACGTGGAACACCACATAATTTTCACTTTTGTTTGCAAATTCACT
CAATGTTTGCATATAANAAAGGTGATACTTTCTATTTTAATGGTGATGATGATGTTTGGG
TATTTATGXXXXXXXXXXAGTTGTTTATACAATTATACTGAATCAACATGTGATGACAAT
GATGCTTGTACTTCAGATGTATGTACAGAATATGGTTGTGAGCATTATACATATACTGGT
TGTATGAATTGTACTAATGGAGGTTGTTTCACTACTCCAGATACATGTAATCATTATGGT
TGTAATTTCACTGGTGATGGTAAATGCTTCTTCACTCATTTAGAATGTGATGATAATGAC
CCATGTACTAAAGATCATTGCTTATATTCTGGGGATTGTTATTATGAAAAACTTACCAAT
TGTACAACCCCAACCCCAACTCAAACTCCTCCTCCAACTCAAACTCCTACTAATCATTCT
ATTGGTGTTAATGAATGCGATTGTTGCCCTGAAGGTCAATATTGTTTATTGATTTTTGGA
CATGAAAGATGTTTCATTGCAAATGATGGTGGTGACGGTATTCCAGAGGAAACTATTGGT
TGTCCTGGTGTAACAACTGGTACACCAACATCAACAGATGGTGGTACAGGTCACTACACT
GAATCAGGTACAGGTAATCCACATTTATGTGATAGACATCATTGTAGGAGTGGAATGGAA
TGTCATGTTATTAATGGAATTCCAGAATGTTTACCATCAAAATACAAATGTTTAGATTGT
TTAGATCTTCATTGTGAAAAACAAGGTGGAAAAACATGTTTTACCATNAAAATCAAAATT
ATAAACCAAATATTAAAGGATGTAAAGANATTCTTGTTGTAAATATACTCCAACTT
sequence update 2002.10.25
Translated Amino Acid sequence 
yyk*IMMKYIYILLIFSLLFLKINSQNTILLNALIYDQTPSRNNDFQIPSPGGIAKGLVK
SNLGSDNTPVLVAERSSTIHSPETFNQWFHNYPGVNLPINYQITLTQSTTNPNVYAYTND
AFFPIDGQGFDNKTNYPYEAVYRDERGTPHNFHFCLQIHSMFAYXKGDTFYFNGDDDVWV
FM---

---SCLYNYTESTCDDNDACTSDVCTEYGCEHYTYTGCMNCTNGGCFTTPDTCNHYGCNF
TGDGKCFFTHLECDDNDPCTKDHCLYSGDCYYEKLTNCTTPTPTQTPPPTQTPTNHSIGV
NECDCCPEGQYCLLIFGHERCFIANDGGDGIPEETIGCPGVTTGTPTSTDGGTGHYTESG
TGNPHLCDRHHCRSGMECHVINGIPECLPSKYKCLDCLDLHCEKQGGKTCFTXKIKIINQ
ILKDVKXFLL*iysn
Translated Amino Acid sequence (All Frames) 
Frame A:
niink***niyify*yflycf*k*ihkiqfc*mh*fmiklhqeimifkfllqevlqkg**
nli*vliihqf*wlsdhqqfihqkllingftiiqe*iyqliikll*lkvlqiqmfmhiqm
mpffq*mdkdliirqiihmkpyiemnvehhiiftfvckftqclhixkvilsilmvmmmfg
yl---

---SCLYNYTESTCDDNDACTSDVCTEYGCEHYTYTGCMNCTNGGCFTTPDTCNHYGCNF
TGDGKCFFTHLECDDNDPCTKDHCLYSGDCYYEKLTNCTTPTPTQTPPPTQTPTNHSIGV
NECDCCPEGQYCLLIFGHERCFIANDGGDGIPEETIGCPGVTTGTPTSTDGGTGHYTESG
TGNPHLCDRHHCRSGMECHVINGIPECLPSKYKCLDCLDLHCEKQGGKTCFTXKIKIINQ
ILKDVKXFLL*iysn

Frame B:
il*inndeiyiyfiniffivfenkftkynfvecinl*snsikk**fsnsfsrryckrvsk
i*frf**ytsfsg*aiinnsftrnf*smvsqlsrskftn*lsnyfnskyykskclciyk*
clfsnrwtri***dklsi*sri*r*twntt*fsllfanslnvci*xr*yflf*w***clg
iy---

---vvytiilnqhvmtmmlvlqmyvqnmvvsiihilvv*ivlmevvsllqihviimvvis
lvmvnassli*nvmimthvlkiiayilgivimknlpivqpqpqlklllqlkllliillvl
mnaivalkvnivy*fldmkdvslqmmvvtvfqrkllvvlv*qlvhqhqqmvvqvttlnqv
qvihiyvidiivgvewnvmllmefqnvyhqntnv*iv*ifivknkvekhvlpxkskl*tk
y*rm*rxscckytpt

Frame C:
yyk*IMMKYIYILLIFSLLFLKINSQNTILLNALIYDQTPSRNNDFQIPSPGGIAKGLVK
SNLGSDNTPVLVAERSSTIHSPETFNQWFHNYPGVNLPINYQITLTQSTTNPNVYAYTND
AFFPIDGQGFDNKTNYPYEAVYRDERGTPHNFHFCLQIHSMFAYXKGDTFYFNGDDDVWV
FM---

---lfiqly*inm**q*clyfrcmyriwl*alyiywlyely*wrlfhysrym*slwl*fh
w*w*mllhsfrm****pmy*rsllifwglll*ktyqlynpnpnsnsssnsnsy*sfywc*
*mrllp*rsilfidfwt*kmfhck*ww*rysrgnywlswcnnwytninrwwyrslh*iry
r*stfm**tsl*ewngmscy*wnsrmftikiqmfrlfrssl*ktrwknmfyhxnqnykpn
ikgckxilvvnilql
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHG225 (SHG225Q) /CSM/SH/SHG2-B/SHG225Q.Seq.d/                       2405   0.0
SHG812 (SHG812Q) /CSM/SH/SHG8-A/SHG812Q.Seq.d/                       1463   0.0
AHQ868 (AHQ868Q) /CSM/AH/AHQ8-C/AHQ868Q.Seq.d/                       1463   0.0
AHO411 (AHO411Q) /CSM/AH/AHO4-A/AHO411Q.Seq.d/                       1463   0.0
AHO377 (AHO377Q) /CSM/AH/AHO3-D/AHO377Q.Seq.d/                       1463   0.0
AHO161 (AHO161Q) /CSM/AH/AHO1-C/AHO161Q.Seq.d/                       1463   0.0
AHN366 (AHN366Q) /CSM/AH/AHN3-C/AHN366Q.Seq.d/                       1463   0.0
AHL621 (AHL621Q) /CSM/AH/AHL6-A/AHL621Q.Seq.d/                       1463   0.0
AHL540 (AHL540Q) /CSM/AH/AHL5-B/AHL540Q.Seq.d/                       1463   0.0
AHL470 (AHL470Q) /CSM/AH/AHL4-C/AHL470Q.Seq.d/                       1463   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC117081|AC117081.2 Dictyostelium discoideum chromosome 2 map 5862124-6045772 strain AX4, complete sequence.    58   5e-04  1
AJ548837|AJ548837.1 Dictyostelium discoideum mRNA for extracellular signalling molecule  DicA (dicA gene).    52   0.002  2
AJ548838|AJ548838.1 Dictyostelium discoideum dicA gene for extracellular signalling  molecule DicA, exons 1-2.    52   0.003  2
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.    52   0.033  1
AB053203|AB053203.1 Dictyostelium discoideum psiA mRNA for Psi facor, complete cds.    46   0.038  3
U67940|U67940.1 Dictyostelium discoideum ORFveg106 mRNA, complete cds.    50   0.13   1
BX510642|BX510642.5 Zebrafish DNA sequence *** SEQUENCING CANCELLED *** from clone  CH211-118B5.    48   0.51   1
BX166864|BX166864.1 Danio rerio genomic clone DKEY-145I7, genomic survey sequence.    48   0.51   1
AL954679|AL954679.7 Zebrafish DNA sequence from clone DKEY-63J12 in linkage group 20.    48   0.51   1
AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.    38   0.70   8
dna update 2006. 3.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54C32) RecName: Full=Protein psiQ; Flags: Precursor;                446   e-127
(Q54C31) RecName: Full=Protein psiR; Flags: Precursor;                229   2e-58
(Q1ZXC5) RecName: Full=Protein psiC; Flags: Precursor;                161   4e-38
(Q54FD8) RecName: Full=Protein psiD; Flags: Precursor;                145   4e-33
(Q54CH8) RecName: Full=Protein psiN; Flags: Precursor;                126   1e-27
(Q54G85) RecName: Full=Protein psiJ; Flags: Precursor;                122   3e-26
(Q968Z6) RecName: Full=Protein psiA; AltName: Full=Prespore-cell...   112   2e-23
(Q55FQ4) RecName: Full=Protein psiB; Flags: Precursor;                106   2e-21
AM746676_5746(AM746676|pid:none) Sorangium cellulosum 'So ce 56'...   104   6e-21
(Q94494) RecName: Full=Protein psiH; Flags: Precursor;  &U67940_...   103   1e-20
protein update 2009. 4.18
PSORT 

 psg: 0.99  gvh: 0.78  alm: 0.51  top: 0.47  tms: 0.00  mit: 0.33  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: extracellular, including cell wall
	 12.0 %: nuclear
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum
	  4.0 %: cytoplasmic
	  4.0 %: mitochondrial
	  4.0 %: Golgi

>> prediction for SHG225 is exc
5' end seq. ID SHG225F
5' end seq. 
>SHG225F.Seq
AATATTATAAATAAATAATGATGAAATATATATATATTTTATTAATATTTTCTTTATTGT
TTTTGAAAATAAATTCACAAAATACAATTTTGTTGAATGCATTAATTTATGATCAAACTC
CATCAAGAAATAATGATTTTCAAATTCCTTCTCCAGGAGGTATTGCAAAAGGGTTAGTAA
AATCTAATTTAGGTTCTGATAATACACCAGTTTTAGTGGCTGAGCGATCATCAACAATTC
ATTCACCAGAAACTTTTAATCAATGGTTTCACAATTATCCAGGAGTAAATTTACCAATTA
ATTATCAAATTACTTTAACTCAAAGTACTACAAATCCAAATGTTTATGCATATACAAATG
ATGCCTTTTTTCCAATAGATGGACAAGGATTTGATAATAAGACAAATTATCCATATGAAG
CCGTATATAGAGATGAACGTGGAACACCACATAATTTTCACTTTTGTTTGCAAATTCACT
CAATGTTTGCATATAANAAAGGTGATACTTTCTATTTTAATGGTGATGATGATGTTTGGG
TATTTATGNNNNNNNNNN
Length of 5' end seq. 558
3' end seq. ID SHG225Z
3' end seq. 
>SHG225Z.Seq
NNNNNNNNNNAGTTGTTTATACAATTATACTGAATCAACATGTGATGACAATGATGCTTG
TACTTCAGATGTATGTACAGAATATGGTTGTGAGCATTATACATATACTGGTTGTATGAA
TTGTACTAATGGAGGTTGTTTCACTACTCCAGATACATGTAATCATTATGGTTGTAATTT
CACTGGTGATGGTAAATGCTTCTTCACTCATTTAGAATGTGATGATAATGACCCATGTAC
TAAAGATCATTGCTTATATTCTGGGGATTGTTATTATGAAAAACTTACCAATTGTACAAC
CCCAACCCCAACTCAAACTCCTCCTCCAACTCAAACTCCTACTAATCATTCTATTGGTGT
TAATGAATGCGATTGTTGCCCTGAAGGTCAATATTGTTTATTGATTTTTGGACATGAAAG
ATGTTTCATTGCAAATGATGGTGGTGACGGTATTCCAGAGGAAACTATTGGTTGTCCTGG
TGTAACAACTGGTACACCAACATCAACAGATGGTGGTACAGGTCACTACACTGAATCAGG
TACAGGTAATCCACATTTATGTGATAGACATCATTGTAGGAGTGGAATGGAATGTCATGT
TATTAATGGAATTCCAGAATGTTTACCATCAAAATACAAATGTTTAGATTGTTTAGATCT
TCATTGTGAAAAACAAGGTGGAAAAACATGTTTTACCATNAAAATCAAAATTATAAACCA
AATATTAAAGGATGTAAAGANATTCTTGTTGTAAATATACTCCAACTT
Length of 3' end seq. 768
Connected seq. ID SHG225P
Connected seq. 
>SHG225P.Seq
AATATTATAAATAAATAATGATGAAATATATATATATTTTATTAATATTTTCTTTATTGT
TTTTGAAAATAAATTCACAAAATACAATTTTGTTGAATGCATTAATTTATGATCAAACTC
CATCAAGAAATAATGATTTTCAAATTCCTTCTCCAGGAGGTATTGCAAAAGGGTTAGTAA
AATCTAATTTAGGTTCTGATAATACACCAGTTTTAGTGGCTGAGCGATCATCAACAATTC
ATTCACCAGAAACTTTTAATCAATGGTTTCACAATTATCCAGGAGTAAATTTACCAATTA
ATTATCAAATTACTTTAACTCAAAGTACTACAAATCCAAATGTTTATGCATATACAAATG
ATGCCTTTTTTCCAATAGATGGACAAGGATTTGATAATAAGACAAATTATCCATATGAAG
CCGTATATAGAGATGAACGTGGAACACCACATAATTTTCACTTTTGTTTGCAAATTCACT
CAATGTTTGCATATAANAAAGGTGATACTTTCTATTTTAATGGTGATGATGATGTTTGGG
TATTTATG----------AGTTGTTTATACAATTATACTGAATCAACATGTGATGACAAT
GATGCTTGTACTTCAGATGTATGTACAGAATATGGTTGTGAGCATTATACATATACTGGT
TGTATGAATTGTACTAATGGAGGTTGTTTCACTACTCCAGATACATGTAATCATTATGGT
TGTAATTTCACTGGTGATGGTAAATGCTTCTTCACTCATTTAGAATGTGATGATAATGAC
CCATGTACTAAAGATCATTGCTTATATTCTGGGGATTGTTATTATGAAAAACTTACCAAT
TGTACAACCCCAACCCCAACTCAAACTCCTCCTCCAACTCAAACTCCTACTAATCATTCT
ATTGGTGTTAATGAATGCGATTGTTGCCCTGAAGGTCAATATTGTTTATTGATTTTTGGA
CATGAAAGATGTTTCATTGCAAATGATGGTGGTGACGGTATTCCAGAGGAAACTATTGGT
TGTCCTGGTGTAACAACTGGTACACCAACATCAACAGATGGTGGTACAGGTCACTACACT
GAATCAGGTACAGGTAATCCACATTTATGTGATAGACATCATTGTAGGAGTGGAATGGAA
TGTCATGTTATTAATGGAATTCCAGAATGTTTACCATCAAAATACAAATGTTTAGATTGT
TTAGATCTTCATTGTGAAAAACAAGGTGGAAAAACATGTTTTACCATNAAAATCAAAATT
ATAAACCAAATATTAAAGGATGTAAAGANATTCTTGTTGTAAATATACTCCAACTT
Length of connected seq. 1306
Full length Seq ID -
Full length Seq. -
Length of full length seq. -