>SHG221 (SHG221Q) /CSM/SH/SHG2-A/SHG221Q.Seq.d/
AATTTTAATTTATATAAATTACCTTATTACTTAAAGATGACAACAAATAATCCATTTGAT
AAAGTAAAGGATGTATTAAAATCACAAGATCAAACTTATAATTTTTATAATCTTTCAAAA
CTTCAAGACCCAAGAATTGAAAAATTACCATACTCCATTAGAATTCTTTTGGAATCAGCA
GTTCGTAATTGTGATAACTTTGAAGTACACGAAAAAGATGTTGAAAATATCTTAAATTGG
GAAAACACTGCAAATAAAGTTGAAATTCCTTTCAAACCAGCAAGAGTATTATTACAAGAT
TTTACTGGTGTACCAGCAGTTGTTGATCTTGCAGCAATGAGAGATGCCATGAAAAGATTA
GGTGGTGATCCAGCAAAAATTAACCCACTTGTTCCAGTTGATTTAGTTATTGATCACTCT
GTTCAAGTTGATGTATCAAGAACTGTTGATGCTTTAGAGCAAAATCAAAAGATTGAATTC
CATAGAAATCATGAAAGATTTTCATTTTTAAAATGGGGTGCTCAAGCATTTGATGGATTA
TTTATTGCTCCACCTGGAAGTGGTATTGTACATCAAGTAAATTTAGAGTATATTGCAAGA
GAAGTTATGAATGGAACTGGTAACCTCTTATGTCCGGATAGTGTTGGTTGGTACTGATTC
TCATACAACAATGATTAATGGTTTAAGTGTTTGTGGTXXXXXXXXXXCTTGGTGATAGTA
TTACCACTGATCATATTTCACCAGCCGGTAACATCAATAGAAAATCATCAGCCGCTCGTT
ATTTAGAGTCCAAAGGTGTAAAACCAGAAGATTTTAACACCTATGGATCTCGTAGAGGTA
ATGATGAAATTATGGTTAGAGGTACCTTTGCCAATACTCGTATTGTTAATAAATTGGCTC
CAGCCGTTGGTCCACAAACAACCTATGTACCAACTGGTGAGTTAATGTTTATTTCAGATG
CTGCAGAAAAATATCAATCAGAAGGTCATCAACTCATTGTTTTGGCTGGTAGTGATTATG
GCTCTGGTAGTTCTAGAGATTGGGCTGCCAAAGGTCCATACCTTCAAGGAATTAAGTGTG
TCATTGCCATCTCATTTGAACGTATTCATAGATCCAATCTCGTTGGTATGGGTATTATCC
CATTGCAATTCCAACCAGGTCAAAATGCCTCTACTTTGGGTCTCACTGGTAAAGAACAAT
TCAATATCGAGTTACCAACTGATAAATCCCTCATTAAAACTGGTCAAACCGTAAAGGTTA
CCACTAATTGTGGTAAATCTTTTGAAACTATCCTTCGTTTCGATACTCCAATCGAAGTTG
AATATTGGGCAAATAATGGTATCTTATCTTATGTTTTAAGAAAACTTTTACATTAATTTC
TTTTTTAATATAAAAAAAAAAAAAA

iliyinylit*r*qqiihlik*rmy*nhkikliifiifqnfktqelknyhtpleffwnqq
fvivitlkytkkmlkis*igktlqiklkflsnqqeyyykillvyqqllilqq*emp*kd*
vviqqklthlfqli*llitlfklmyqellml*skikrlnsieimkdfhf*ngvlkhlmdy
lllhlevvlyik*i*silqekl*MELVTSYVRIVLVGTDSHTTMINGLSVCG---

---LGDSITTDHISPAGNINRKSSAARYLESKGVKPEDFNTYGSRRGNDEIMVRGTFANT
RIVNKLAPAVGPQTTYVPTGELMFISDAAEKYQSEGHQLIVLAGSDYGSGSSRDWAAKGP
YLQGIKCVIAISFERIHRSNLVGMGIIPLQFQPGQNASTLGLTGKEQFNIELPTDKSLIK
TGQTVKVTTNCGKSFETILRFDTPIEVEYWANNGILSYVLRKLLH*flf*ykkkk

Frame A:
nfnlyklpyylkmttnnpfdkvkdvlksqdqtynfynlsklqdprieklpysirillesa
vrncdnfevhekdvenilnwentankveipfkparvllqdftgvpavvdlaamrdamkrl
ggdpakinplvpvdlvidhsvqvdvsrtvdaleqnqkiefhrnherfsflkwgaqafdgl
fiappgsgivhqvnleyiarevmngtgnllcpdsvgwy*fsynnd*wfkclw---

---LGDSITTDHISPAGNINRKSSAARYLESKGVKPEDFNTYGSRRGNDEIMVRGTFANT
RIVNKLAPAVGPQTTYVPTGELMFISDAAEKYQSEGHQLIVLAGSDYGSGSSRDWAAKGP
YLQGIKCVIAISFERIHRSNLVGMGIIPLQFQPGQNASTLGLTGKEQFNIELPTDKSLIK
TGQTVKVTTNCGKSFETILRFDTPIEVEYWANNGILSYVLRKLLH*flf*ykkkk

Frame B:
iliyinylit*r*qqiihlik*rmy*nhkikliifiifqnfktqelknyhtpleffwnqq
fvivitlkytkkmlkis*igktlqiklkflsnqqeyyykillvyqqllilqq*emp*kd*
vviqqklthlfqli*llitlfklmyqellml*skikrlnsieimkdfhf*ngvlkhlmdy
lllhlevvlyik*i*silqekl*MELVTSYVRIVLVGTDSHTTMINGLSVCG---

---lvivlpliifhqpvtsienhqplvi*spkv*nqkiltpmdlvevmmklwlevplpil
vllinwlqplvhkqpmyqlvs*clfqmlqkninqkvinslfwlvvimalvvleiglpkvh
tfkelsvslpshlnvfidpislvwvlshcnsnqvkmpllwvslvknnsissyqlinpslk
lvkp*rlplivvnllklsfvsilqsklnigqimvsylmf*enfyinfffnikkkk

Frame C:
f*fi*itlllkddnk*si**skgcikitrsnl*fl*sfktsrpkn*kitilh*nsfgiss
s*l**l*strkrc*kylklgkhck*s*nsfqtsksiitrfywctssc*scsnerchekir
w*sskn*ptcss*fsy*slcss*ciknc*cfrakskd*ip*ks*kififkmgcssi*wii
ycstwkwyctsskfrvyckrsyewnw*plmsg*cwlvliliqq*lmv*vfv---

---w**yyh*syftsr*hq*kiisrslfrvqrcktrrf*hlwis*r***nyg*rylcqys
yc**igssrwstnnlctnw*vnvyfrccrkisirrssthcfgw**lwlw*f*rlgcqrsi
psrn*vchchli*tys*iqsrwygyypiaiptrskclyfgshw*rtiqyrvtn**iph*n
wsnrkgyh*lw*if*nypsfrysnrs*ilgk*wylilcfkktftlisfli*kkkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHG221 (SHG221Q) /CSM/SH/SHG2-A/SHG221Q.Seq.d/                       2698   0.0
VHA182 (VHA182Q) /CSM/VH/VHA1-D/VHA182Q.Seq.d/                       1356   0.0
VHA161 (VHA161Q) /CSM/VH/VHA1-C/VHA161Q.Seq.d/                       1356   0.0
SHA509 (SHA509Q) /CSM/SH/SHA5-A/SHA509Q.Seq.d/                       1356   0.0
CHQ778 (CHQ778Q) /CSM/CH/CHQ7-D/CHQ778Q.Seq.d/                       1356   0.0
CHL182 (CHL182Q) /CSM/CH/CHL1-D/CHL182Q.Seq.d/                       1356   0.0
AHO171 (AHO171Q) /CSM/AH/AHO1-C/AHO171Q.Seq.d/                       1356   0.0
AHF254 (AHF254Q) /CSM/AH/AHF2-C/AHF254Q.Seq.d/                       1356   0.0
AHD288 (AHD288Q) /CSM/AH/AHD2-D/AHD288Q.Seq.d/                       1356   0.0
AHC891 (AHC891Q) /CSM/AH/AHC8-D/AHC891Q.Seq.d/                       1356   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF194945|AF194945.1 Nicotiana tabacum cytosolic aconitase (ACO) mRNA, complete cds.    66   1e-33  6
AY250116|AY250116.1 Lycopersicon pennellii aconitase mRNA, partial cds.    60   7e-26  5
AY250115|AY250115.1 Lycopersicon pennellii aconitase mRNA, partial cds.    60   2e-21  5
D29629|D29629.1 Cucurbita cv. Kurokawa Amakuri mRNA for aconitase, complete cds.    54   1e-20  4
BE579347|BE579347.1 kq26c09.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to WP:ZK455.1 CE03812 PROBABLE ACONITATE HYDRATASE, CYTOPLASMIC ;, mRNA sequence.    64   3e-20  3
AF073507|AF073507.1 Citrus limon aconitase-iron regulated protein 1 (IRP1) mRNA, complete cds.    56   1e-19  7
BI925204|BI925204.1 EST545093 tomato flower, buds 0-3 mm Lycopersicon esculentum cDNA clone cTOA25H12 5' end, mRNA sequence.    58   8e-19  3
BI930520|BI930520.1 EST550409 tomato flower, 8 mm to preanthesis buds Lycopersicon esculentum cDNA clone cTOC16J14 5' end, mRNA sequence.    58   9e-19  3
CO910019|CO910019.1 BJ02055G09 BJ02 Capsicum annuum cDNA 5', mRNA sequence.    58   2e-18  4
DV184073|DV184073.1 CT030_H12_CT030_3700_91.ab1 C. tentans tissue culture cDNA library Chironomus tentans cDNA, mRNA sequence.    60   3e-18  5

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54X73) RecName: Full=Probable cytoplasmic aconitate hydratase;...   435   e-120
EU816775_1(EU816775|pid:none) Perkinsus marinus aconitase-1 mRNA...   279   3e-77
AB236788_1(AB236788|pid:none) Trifolium pratense RNA for putativ...   288   2e-76
AP008209_256(AP008209|pid:none) Oryza sativa (japonica cultivar-...   287   7e-76
BT060665_1(BT060665|pid:none) Zea mays full-length cDNA clone ZM...   285   2e-75
A45785_1(A45785|pid:none) Sequence 23 from Patent WO9520046.  &X...   274   2e-75
BT060820_1(BT060820|pid:none) Zea mays full-length cDNA clone ZM...   285   3e-75
(Q23500) RecName: Full=Probable cytoplasmic aconitate hydratase;...   285   3e-75
(P49608) RecName: Full=Aconitate hydratase, cytoplasmic;        ...   281   3e-74
(Q9SIB9) RecName: Full=Aconitate hydratase 2, mitochondrial;    ...   281   4e-74

 psg: 0.80  gvh: 0.45  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 36.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for SHG221 is nuc

>SHG221F.Seq
AATTTTAATTTATATAAATTACCTTATTACTTAAAGATGACAACAAATAATCCATTTGAT
AAAGTAAAGGATGTATTAAAATCACAAGATCAAACTTATAATTTTTATAATCTTTCAAAA
CTTCAAGACCCAAGAATTGAAAAATTACCATACTCCATTAGAATTCTTTTGGAATCAGCA
GTTCGTAATTGTGATAACTTTGAAGTACACGAAAAAGATGTTGAAAATATCTTAAATTGG
GAAAACACTGCAAATAAAGTTGAAATTCCTTTCAAACCAGCAAGAGTATTATTACAAGAT
TTTACTGGTGTACCAGCAGTTGTTGATCTTGCAGCAATGAGAGATGCCATGAAAAGATTA
GGTGGTGATCCAGCAAAAATTAACCCACTTGTTCCAGTTGATTTAGTTATTGATCACTCT
GTTCAAGTTGATGTATCAAGAACTGTTGATGCTTTAGAGCAAAATCAAAAGATTGAATTC
CATAGAAATCATGAAAGATTTTCATTTTTAAAATGGGGTGCTCAAGCATTTGATGGATTA
TTTATTGCTCCACCTGGAAGTGGTATTGTACATCAAGTAAATTTAGAGTATATTGCAAGA
GAAGTTATGAATGGAACTGGTAACCTCTTATGTCCGGATAGTGTTGGTTGGTACTGATTC
TCATACAACAATGATTAATGGTTTAAGTGTTTGTGGTNNNNNNNNNN

>SHG221Z.Seq
NNNNNNNNNNCTTGGTGATAGTATTACCACTGATCATATTTCACCAGCCGGTAACATCAA
TAGAAAATCATCAGCCGCTCGTTATTTAGAGTCCAAAGGTGTAAAACCAGAAGATTTTAA
CACCTATGGATCTCGTAGAGGTAATGATGAAATTATGGTTAGAGGTACCTTTGCCAATAC
TCGTATTGTTAATAAATTGGCTCCAGCCGTTGGTCCACAAACAACCTATGTACCAACTGG
TGAGTTAATGTTTATTTCAGATGCTGCAGAAAAATATCAATCAGAAGGTCATCAACTCAT
TGTTTTGGCTGGTAGTGATTATGGCTCTGGTAGTTCTAGAGATTGGGCTGCCAAAGGTCC
ATACCTTCAAGGAATTAAGTGTGTCATTGCCATCTCATTTGAACGTATTCATAGATCCAA
TCTCGTTGGTATGGGTATTATCCCATTGCAATTCCAACCAGGTCAAAATGCCTCTACTTT
GGGTCTCACTGGTAAAGAACAATTCAATATCGAGTTACCAACTGATAAATCCCTCATTAA
AACTGGTCAAACCGTAAAGGTTACCACTAATTGTGGTAAATCTTTTGAAACTATCCTTCG
TTTCGATACTCCAATCGAAGTTGAATATTGGGCAAATAATGGTATCTTATCTTATGTTTT
AAGAAAACTTTTACATTAATTTCTTTTTTAATATAAAAAAAAAAAAAA

>SHG221P.Seq
AATTTTAATTTATATAAATTACCTTATTACTTAAAGATGACAACAAATAATCCATTTGAT
AAAGTAAAGGATGTATTAAAATCACAAGATCAAACTTATAATTTTTATAATCTTTCAAAA
CTTCAAGACCCAAGAATTGAAAAATTACCATACTCCATTAGAATTCTTTTGGAATCAGCA
GTTCGTAATTGTGATAACTTTGAAGTACACGAAAAAGATGTTGAAAATATCTTAAATTGG
GAAAACACTGCAAATAAAGTTGAAATTCCTTTCAAACCAGCAAGAGTATTATTACAAGAT
TTTACTGGTGTACCAGCAGTTGTTGATCTTGCAGCAATGAGAGATGCCATGAAAAGATTA
GGTGGTGATCCAGCAAAAATTAACCCACTTGTTCCAGTTGATTTAGTTATTGATCACTCT
GTTCAAGTTGATGTATCAAGAACTGTTGATGCTTTAGAGCAAAATCAAAAGATTGAATTC
CATAGAAATCATGAAAGATTTTCATTTTTAAAATGGGGTGCTCAAGCATTTGATGGATTA
TTTATTGCTCCACCTGGAAGTGGTATTGTACATCAAGTAAATTTAGAGTATATTGCAAGA
GAAGTTATGAATGGAACTGGTAACCTCTTATGTCCGGATAGTGTTGGTTGGTACTGATTC
TCATACAACAATGATTAATGGTTTAAGTGTTTGTGGT----------CTTGGTGATAGTA
TTACCACTGATCATATTTCACCAGCCGGTAACATCAATAGAAAATCATCAGCCGCTCGTT
ATTTAGAGTCCAAAGGTGTAAAACCAGAAGATTTTAACACCTATGGATCTCGTAGAGGTA
ATGATGAAATTATGGTTAGAGGTACCTTTGCCAATACTCGTATTGTTAATAAATTGGCTC
CAGCCGTTGGTCCACAAACAACCTATGTACCAACTGGTGAGTTAATGTTTATTTCAGATG
CTGCAGAAAAATATCAATCAGAAGGTCATCAACTCATTGTTTTGGCTGGTAGTGATTATG
GCTCTGGTAGTTCTAGAGATTGGGCTGCCAAAGGTCCATACCTTCAAGGAATTAAGTGTG
TCATTGCCATCTCATTTGAACGTATTCATAGATCCAATCTCGTTGGTATGGGTATTATCC
CATTGCAATTCCAACCAGGTCAAAATGCCTCTACTTTGGGTCTCACTGGTAAAGAACAAT
TCAATATCGAGTTACCAACTGATAAATCCCTCATTAAAACTGGTCAAACCGTAAAGGTTA
CCACTAATTGTGGTAAATCTTTTGAAACTATCCTTCGTTTCGATACTCCAATCGAAGTTG
AATATTGGGCAAATAATGGTATCTTATCTTATGTTTTAAGAAAACTTTTACATTAATTTC
TTTTTTAATATAAAAAAAAAAAAAA