>SHG190 (SHG190Q) /CSM/SH/SHG1-D/SHG190Q.Seq.d/
ATAATAAAAAACGAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGG
TCAAAATGAAGAATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGA
GCAACAATTAACTAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGAT
AGAAGAGGCATTAGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTC
AATTTATAATAAACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCATAA
AATTTTCAATAAAGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTAAA
AGAAACAGCAGAAAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGA
ATCATCAGAAGGTGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTT
ATCAGCATTTGTAAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATC
GATCTATCAATCACAAAAGAATATACATACACAATTCTTTAAGAATGTTCATTTACAAGC
TGTCTATTATTCAATGATTGATATATTTTCAGTATTAATAAATTTAGXXXXXXXXXXTGA
CAGAGTTGGAAAGAGACATTAGACAATTGGGTGTCTCCGAGACTGGTTTAACCTTTTTAG
ATCATTTTAGACTTTTAATCACTCATATTGGTAATGCTATGGGTTATATTAGATTGGTCA
GATCCGGTGGTTTACACTATTGTAGTAATGCTATCAAATTCGTACCAGACTTGAAAAAGA
TTCCAAAATTCCAAGATTTAACCTCAAAAGATGCCCTCTCACCTGAAACAATTCAAGCTA
GTACCAATTTGGATTCAATCATTCATAATCTTTCAAATAATTTATCAGAGGGTACTGAAT
ACTTTAAGATGTTGGTAAACGTTTTCGCTACAGAGTTTCGAAATATCGCTAATCAACATT
TAAAGAATTTCTATATCATCGTACCTGCCTTGGCCTCAAATTACATCGATCATATGATCA
ATAGTAAAGATAAACTCTTTAAGAAATCAAAAGCAATCGGTGCTGAAGCTTTATTATTCA
CTGATGATGGCTTTGCAATTGGTTTGGCTTATATCTTAAAATTATTAGATCAAAATAAAG
ATTTCGATTCTCTTCATTGGTTCGATCGTATTCAATTGAAATGTGAAACTGATCAAAAGA
GAATGTTGGCTGAAGCAAATGCAAAAGGTATCAAAGAGGATCAAAATCATTTAGCAATTA
AAAAGATTCAAAATTTCCAACAAGAATAGAATTATTTAAATATTCTTTCTCGGTTCAAG

**ktnnpi*KMATSYSANNGQNEEYTFDAFEDGSSKLVGEQQLTKLNQFAVDYAQQIWKI
EEALDETLNEVWDINIDPVSIYNKPHEQLDLIELVKTDHKIFNKVILVFSSICNQTRILK
ETAESKFYSPLTVFGEITGESSEGDVQIEVGKLLPFMIDLSAFVNRCYSLIRNIISQFAS
IYQSQKNIHTQFFKNVHLQAVYYSMIDIFSVLINL---

---TELERDIRQLGVSETGLTFLDHFRLLITHIGNAMGYIRLVRSGGLHYCSNAIKFVPD
LKKIPKFQDLTSKDALSPETIQASTNLDSIIHNLSNNLSEGTEYFKMLVNVFATEFRNIA
NQHLKNFYIIVPALASNYIDHMINSKDKLFKKSKAIGAEALLFTDDGFAIGLAYILKLLD
QNKDFDSLHWFDRIQLKCETDQKRMLAEANAKGIKEDQNHLAIKKIQNFQQE*nylnils
rfk

Frame A:
iikneqpnlenghilfg**wsk*riyi*ci*rwfi*igwratin*t*sics*lcttnled
rrgir*nik*smgykyrssfnl**ts*tirfn*ig*n*s*nfq*snisilfnl*sn*nfk
rnsrk*ilftinsiwrdyr*iirr*ctn*sw*iitiyd*fisick*mlfin*kyy*sici
dlsitkeytytil*ecsftscllfnd*yifsinkf---

---*qswketldnwvsprlv*pf*iildf*slilvmlwvildwsdpvvytivvmlsnsyq
t*krfqnski*pqkmpshlkqfklvpiwiqsfiifqiiyqrvlntlrcw*tfslqsfeis
lini*risissylpwpqitsii*sivkinslrnqkqsvlklyyslmmalqlvwlis*ny*
ikikisilfigsivfn*nvklikrecwlkqmqkvskrikii*qlkrfkisnknrii*iff
lgs

Frame B:
**ktnnpi*KMATSYSANNGQNEEYTFDAFEDGSSKLVGEQQLTKLNQFAVDYAQQIWKI
EEALDETLNEVWDINIDPVSIYNKPHEQLDLIELVKTDHKIFNKVILVFSSICNQTRILK
ETAESKFYSPLTVFGEITGESSEGDVQIEVGKLLPFMIDLSAFVNRCYSLIRNIISQFAS
IYQSQKNIHTQFFKNVHLQAVYYSMIDIFSVLINL---

---drvgkrh*tigclrdwfnlfrsf*tfnhsyw*cygly*igqirwftll**cyqirtr
lekdskiprfnlkrcplt*nnss*yqfgfnhs*sfk*firgy*il*dvgkrfryrvskyr
*stfkeflyhrtclglklhrsydq**r*tl*eiksnrc*sfiih**wlcnwfglylkiir
sk*rfrfsslvrsysiem*n*skenvg*skckryqrgsksfsn*kdskfptrielfkysf
svq

Frame C:
nkkrttqfrkwphpirlimvkmknihlmhlkmvhlnwlesnn*lnlinlqlimhnkfgr*
krh*mkh*mkygi*i*iqfqfiinlmnn*i*lnwlkliikfsik*y*yslqfviklef*k
kqqkvnfihh*qylerlqvnhqkvmyklklvnyyhl*liyqhl*idvih*leillvnlhr
sinhkriyihnslrmfiyklsiiq*liyfqy**i*---

---TELERDIRQLGVSETGLTFLDHFRLLITHIGNAMGYIRLVRSGGLHYCSNAIKFVPD
LKKIPKFQDLTSKDALSPETIQASTNLDSIIHNLSNNLSEGTEYFKMLVNVFATEFRNIA
NQHLKNFYIIVPALASNYIDHMINSKDKLFKKSKAIGAEALLFTDDGFAIGLAYILKLLD
QNKDFDSLHWFDRIQLKCETDQKRMLAEANAKGIKEDQNHLAIKKIQNFQQE*nylnils
rfk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHG190 (SHG190Q) /CSM/SH/SHG1-D/SHG190Q.Seq.d/                       2674   0.0
VHP871 (VHP871Q) /CSM/VH/VHP8-C/VHP871Q.Seq.d/                       1425   0.0
VHK424 (VHK424Q) /CSM/VH/VHK4-A/VHK424Q.Seq.d/                       1425   0.0
VHJ568 (VHJ568Q) /CSM/VH/VHJ5-C/VHJ568Q.Seq.d/                       1425   0.0
VHI654 (VHI654Q) /CSM/VH/VHI6-C/VHI654Q.Seq.d/                       1425   0.0
VHI518 (VHI518Q) /CSM/VH/VHI5-A/VHI518Q.Seq.d/                       1425   0.0
VHB614 (VHB614Q) /CSM/VH/VHB6-A/VHB614Q.Seq.d/                       1425   0.0
VHA684 (VHA684Q) /CSM/VH/VHA6-D/VHA684Q.Seq.d/                       1425   0.0
CHG805 (CHG805Q) /CSM/CH/CHG8-A/CHG805Q.Seq.d/                       1425   0.0
CHG615 (CHG615Q) /CSM/CH/CHG6-A/CHG615Q.Seq.d/                       1425   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BU746157|BU746157.1 CH3#002_C07T7 Canine heart normalized cDNA Library in pBluescriptCanis familiaris cDNA clone CH3#002_C07 5', mRNA sequence.    38   7e-04  3
BY464171|BY464171.1 Mus musculus 15 days pregnant adult female amnion cDNA, RIKEN full-length enriched library, clone:K630150M24, 3' end partial sequence.    42   0.028  2
AK113047|AK113047.1 Ciona intestinalis cDNA, clone:ciad002a21, full insert sequence.    48   0.033  2
AC168241|AC168241.2 Bos taurus clone CH240-197K22, *** SEQUENCING IN PROGRESS ***, 28 unordered pieces.    52   0.035  1
AF063866|AF063866.1 Melanoplus sanguinipes entomopoxvirus, complete genome.    32   0.079  12
CR450713|CR450713.2 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    38   0.098  8
BB822673|BB822673.1 Mus musculus mammary gland RCB-0526 Jyg-MC(A) cDNA, RIKEN full-length enriched library, clone:G830022O04, 3' end partial sequence.    32   0.11   4
DN287505|DN287505.1 1241755 MARC 7BOV Bos taurus cDNA 3', mRNA sequence.    34   0.13   3
CR044130|CR044130.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP186g09.    50   0.14   1
CR034434|CR034434.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP47e19.    50   0.14   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54R74) RecName: Full=UPF0681 protein;                               457   e-127
BC040936_1(BC040936|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   229   1e-58
AB028956_1(AB028956|pid:none) Homo sapiens mRNA for KIAA1033 pro...   229   2e-58
BC143373_1(BC143373|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   229   2e-58
BC020314_1(BC020314|pid:none) Mus musculus RIKEN cDNA A230046K03...   227   6e-58
(Q3UMB9) RecName: Full=UPF0681 protein KIAA1033;                      227   6e-58
AK161247_1(AK161247|pid:none) Mus musculus adult male testis cDN...   227   6e-58
AK145000_1(AK145000|pid:none) Mus musculus mammary gland RCB-052...   227   6e-58
BC110850_1(BC110850|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   174   6e-42
CP000600_221(CP000600|pid:none) Ostreococcus lucimarinus CCE9901...   170   9e-41

 psg: 0.79  gvh: 0.25  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 40.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for SHG190 is nuc

>SHG190F.Seq
ATAATAAAAAACGAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGG
TCAAAATGAAGAATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGA
GCAACAATTAACTAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGAT
AGAAGAGGCATTAGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTC
AATTTATAATAAACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCATAA
AATTTTCAATAAAGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTAAA
AGAAACAGCAGAAAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGA
ATCATCAGAAGGTGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTT
ATCAGCATTTGTAAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATC
GATCTATCAATCACAAAAGAATATACATACACAATTCTTTAAGAATGTTCATTTACAAGC
TGTCTATTATTCAATGATTGATATATTTTCAGTATTAATAAATTTAGNNNNNNNNNN

>SHG190Z.Seq
NNNNNNNNNNTGACAGAGTTGGAAAGAGACATTAGACAATTGGGTGTCTCCGAGACTGGT
TTAACCTTTTTAGATCATTTTAGACTTTTAATCACTCATATTGGTAATGCTATGGGTTAT
ATTAGATTGGTCAGATCCGGTGGTTTACACTATTGTAGTAATGCTATCAAATTCGTACCA
GACTTGAAAAAGATTCCAAAATTCCAAGATTTAACCTCAAAAGATGCCCTCTCACCTGAA
ACAATTCAAGCTAGTACCAATTTGGATTCAATCATTCATAATCTTTCAAATAATTTATCA
GAGGGTACTGAATACTTTAAGATGTTGGTAAACGTTTTCGCTACAGAGTTTCGAAATATC
GCTAATCAACATTTAAAGAATTTCTATATCATCGTACCTGCCTTGGCCTCAAATTACATC
GATCATATGATCAATAGTAAAGATAAACTCTTTAAGAAATCAAAAGCAATCGGTGCTGAA
GCTTTATTATTCACTGATGATGGCTTTGCAATTGGTTTGGCTTATATCTTAAAATTATTA
GATCAAAATAAAGATTTCGATTCTCTTCATTGGTTCGATCGTATTCAATTGAAATGTGAA
ACTGATCAAAAGAGAATGTTGGCTGAAGCAAATGCAAAAGGTATCAAAGAGGATCAAAAT
CATTTAGCAATTAAAAAGATTCAAAATTTCCAACAAGAATAGAATTATTTAAATATTCTT
TCTCGGTTCAAG

>SHG190P.Seq
ATAATAAAAAACGAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGG
TCAAAATGAAGAATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGA
GCAACAATTAACTAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGAT
AGAAGAGGCATTAGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTC
AATTTATAATAAACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCATAA
AATTTTCAATAAAGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTAAA
AGAAACAGCAGAAAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGA
ATCATCAGAAGGTGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTT
ATCAGCATTTGTAAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATC
GATCTATCAATCACAAAAGAATATACATACACAATTCTTTAAGAATGTTCATTTACAAGC
TGTCTATTATTCAATGATTGATATATTTTCAGTATTAATAAATTTAG----------TGA
CAGAGTTGGAAAGAGACATTAGACAATTGGGTGTCTCCGAGACTGGTTTAACCTTTTTAG
ATCATTTTAGACTTTTAATCACTCATATTGGTAATGCTATGGGTTATATTAGATTGGTCA
GATCCGGTGGTTTACACTATTGTAGTAATGCTATCAAATTCGTACCAGACTTGAAAAAGA
TTCCAAAATTCCAAGATTTAACCTCAAAAGATGCCCTCTCACCTGAAACAATTCAAGCTA
GTACCAATTTGGATTCAATCATTCATAATCTTTCAAATAATTTATCAGAGGGTACTGAAT
ACTTTAAGATGTTGGTAAACGTTTTCGCTACAGAGTTTCGAAATATCGCTAATCAACATT
TAAAGAATTTCTATATCATCGTACCTGCCTTGGCCTCAAATTACATCGATCATATGATCA
ATAGTAAAGATAAACTCTTTAAGAAATCAAAAGCAATCGGTGCTGAAGCTTTATTATTCA
CTGATGATGGCTTTGCAATTGGTTTGGCTTATATCTTAAAATTATTAGATCAAAATAAAG
ATTTCGATTCTCTTCATTGGTTCGATCGTATTCAATTGAAATGTGAAACTGATCAAAAGA
GAATGTTGGCTGAAGCAAATGCAAAAGGTATCAAAGAGGATCAAAATCATTTAGCAATTA
AAAAGATTCAAAATTTCCAACAAGAATAGAATTATTTAAATATTCTTTCTCGGTTCAAG