>SHG171 (SHG171Q) /CSM/SH/SHG1-C/SHG171Q.Seq.d/
XXXXXXXXXXCTCTTCCAACTCTTCCAGAACAAAAAGACCAATCTGATGAACCACCCAAT
CTTGTCGATGTTTTAAACAAGGCTCGTCAAGCTGATCCATCTTTGGAGCCAGAAATCAAA
AAGATCCTTGATAACCTCAAAAAACTTGAAGAATATAATCTCACCACCTCTGCCGATAAT
TATTCCTTTTTCCTCAAGGCAGTCGCACTCGAAGTAGTTAATCGTGCTGAAATTCGTGAA
CAACAAAAGAAGGAGAAACAAAGATTAACCACCTCTTTGAATAATCTTCGTAAACATCAA
AAATATCTCAACGAACAAATCGCTCAATACAATCAATACCTCCAAGATTGTCGTCTCAAA
CATTATCAAAACAAGAGCAAAAAGAAGAAGAAGGGAGATGGTGCCAAAGTTGGTCCTTTC
AAATTCAGTTTCAGCGAACTTCACAAGAAAGGTGTCATCGTCGACTCTGAAGTTCCTCAA
ATCACCAGAAAGAAGATTAAATTTGTCATTAGCTCTGATACCGTTGGTGTGTTTGATGTC
TCTGCTAAAATGGCTGGTATCGATGTTCAAACTATGCGTTTGGAACTTGATGACATACTT
GNTCCTCAGTGTCATGTTG

---LPTLPEQKDQSDEPPNLVDVLNKARQADPSLEPEIKKILDNLKKLEEYNLTTSADNY
SFFLKAVALEVVNRAEIREQQKKEKQRLTTSLNNLRKHQKYLNEQIAQYNQYLQDCRLKH
YQNKSKKKKKGDGAKVGPFKFSFSELHKKGVIVDSEVPQITRKKIKFVISSDTVGVFDVS
AKMAGIDVQTMRLELDDILXPQCHV

Frame A:
---ssnssrtkrpi**ttqscrcfkqgsss*sifgarnqkdp**pqkt*ri*shhlcr*l
flfpqgsrtrss*sc*ns*ttkegetkinhlfe*ss*tskisqrtnrsiqsipprlssqt
lskqeqkeeegrwcqswsfqiqfqrtsqerchrrl*sssnhqked*ich*l*yrwcv*cl
c*ngwyrcsnyafgt**htxssvsc


Frame B:
---LPTLPEQKDQSDEPPNLVDVLNKARQADPSLEPEIKKILDNLKKLEEYNLTTSADNY
SFFLKAVALEVVNRAEIREQQKKEKQRLTTSLNNLRKHQKYLNEQIAQYNQYLQDCRLKH
YQNKSKKKKKGDGAKVGPFKFSFSELHKKGVIVDSEVPQITRKKIKFVISSDTVGVFDVS
AKMAGIDVQTMRLELDDILXPQCHV


Frame C:
---fqlfqnkktnlmnhpilsmf*trlvklihlwsqkskrslitsknlkniispplpiii
pfssrqshsk*livlkfvnnkrrrnkd*ppl*iifvniknistnkslntintskivvsni
iktrakrrrremvpklvlsnsvsanftrkvssstlkflksperrlnlslaliplvclmsl
lkwlvsmfklcvwnlmtylxlsvml

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHG171 (SHG171Q) /CSM/SH/SHG1-C/SHG171Q.Seq.d/                       1201   0.0
SHL214 (SHL214Q) /CSM/SH/SHL2-A/SHL214Q.Seq.d/                       1150   0.0
SHL120 (SHL120Q) /CSM/SH/SHL1-A/SHL120Q.Seq.d/                       1150   0.0
SHK836 (SHK836Q) /CSM/SH/SHK8-B/SHK836Q.Seq.d/                       1150   0.0
CHM205 (CHM205Q) /CSM/CH/CHM2-A/CHM205Q.Seq.d/                       1150   0.0
AHN241 (AHN241Q) /CSM/AH/AHN2-B/AHN241Q.Seq.d/                       1150   0.0
AHB795 (AHB795Q) /CSM/AH/AHB7-D/AHB795Q.Seq.d/                       1150   0.0
AHE179 (AHE179Q) /CSM/AH/AHE1-D/AHE179Q.Seq.d/                       1148   0.0
AHE493 (AHE493Q) /CSM/AH/AHE4-D/AHE493Q.Seq.d/                       1144   0.0
VHP536 (VHP536Q) /CSM/VH/VHP5-B/VHP536Q.Seq.d/                       1142   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U81156|U81156.1 Dictyostelium discoideum RasGAP1 (rgaA) gene, complete cds.  1150   0.0    1
L75794|L75794.1 Dictyostelium discoideum RasGTPase-activating protein gene, complete cds.  1150   0.0    1
DT043362|DT043362.1 Mdrtp1011E01.g1 Apple_EST_Mdrtp Malus x domestica x Malus sieversii cDNA, mRNA sequence.    48   0.24   1
BW248505|BW248505.1 Ciona intestinalis cDNA, clone:citb078a01, 5' end, single read.    48   0.24   1
BH552536|BH552536.1 BOHCC63TF BOHC Brassica oleracea genomic clone BOHCC63, DNA sequence.    46   0.94   1
AL161538|AL161538.2 Arabidopsis thaliana DNA chromosome 4, contig fragment No. 38.    42   1.3    3
AL021712|AL021712.2 Arabidopsis thaliana DNA chromosome 4, BAC clone T10I14 (ESSA  project).    38   2.4    2
DN191182|DN191182.1 ACL5_13E12 AcL5 cDNA Library Angiostrongylus cantonensis cDNA, mRNA sequence.    44   3.7    1
DN190596|DN190596.1 ACL5_06E22 AcL5 cDNA Library Angiostrongylus cantonensis cDNA, mRNA sequence.    44   3.7    1
CJ106169|CJ106169.1 Mus musculus cDNA, RIKEN full-length enriched library, clone:M5C1010M18, 5' end partial sequence.    44   3.7    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U81156_1(U81156|pid:none) Dictyostelium discoideum RasGAP1 (rgaA...   336   3e-91
AB076897_1(AB076897|pid:none) Ciona intestinalis Ci-IQGAP mRNA f...    82   9e-15
AK302981_1(AK302981|pid:none) Homo sapiens cDNA FLJ52376 complet...    82   2e-14
AK303054_1(AK303054|pid:none) Homo sapiens cDNA FLJ50398 complet...    82   2e-14
(Q13576) RecName: Full=Ras GTPase-activating-like protein IQGAP2...    82   2e-14
AY351902_1(AY351902|pid:none) Homo sapiens testis specific IQ mo...    82   2e-14
AB093561_1(AB093561|pid:none) Xenopus laevis XIQGAP2 mRNA for IQ...    76   6e-13
BT072767_1(BT072767|pid:none) Salmo salar clone ssal-rgf-539-307...    76   6e-13
BC150958_1(BC150958|pid:none) Mus musculus IQ motif containing G...    74   4e-12
(Q3UQ44) RecName: Full=Ras GTPase-activating-like protein IQGAP2;      74   4e-12

 psg: 0.75  gvh: 0.36  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.18  erl: 0.00  erm: 0.00  pox: 0.30  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 32.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  4.0 %: Golgi

>> prediction for SHG171 is nuc

>SHG171Z.Seq
NNNNNNNNNNCTCTTCCAACTCTTCCAGAACAAAAAGACCAATCTGATGAACCACCCAAT
CTTGTCGATGTTTTAAACAAGGCTCGTCAAGCTGATCCATCTTTGGAGCCAGAAATCAAA
AAGATCCTTGATAACCTCAAAAAACTTGAAGAATATAATCTCACCACCTCTGCCGATAAT
TATTCCTTTTTCCTCAAGGCAGTCGCACTCGAAGTAGTTAATCGTGCTGAAATTCGTGAA
CAACAAAAGAAGGAGAAACAAAGATTAACCACCTCTTTGAATAATCTTCGTAAACATCAA
AAATATCTCAACGAACAAATCGCTCAATACAATCAATACCTCCAAGATTGTCGTCTCAAA
CATTATCAAAACAAGAGCAAAAAGAAGAAGAAGGGAGATGGTGCCAAAGTTGGTCCTTTC
AAATTCAGTTTCAGCGAACTTCACAAGAAAGGTGTCATCGTCGACTCTGAAGTTCCTCAA
ATCACCAGAAAGAAGATTAAATTTGTCATTAGCTCTGATACCGTTGGTGTGTTTGATGTC
TCTGCTAAAATGGCTGGTATCGATGTTCAAACTATGCGTTTGGAACTTGATGACATACTT
GNTCCTCAGTGTCATGTTG