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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHG169
Library SH
(Link to library)
Clone ID SHG169
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10823-1|Contig-U13268-1
Original site URL
Representative seq. ID SHG169P
(Link to Original site)
Representative DNA sequence 
>SHG169 (SHG169Q) /CSM/SH/SHG1-C/SHG169Q.Seq.d/
AGTGTGTTAAAAATATAAACTAAAACTAAAAAAAAAATGAAACTTTTGTCTTCATTAATT
ATTTTTTTTGTTATTGTATTATTTTGNGTTGTTGGATCATTATCAGCATCATTATGTAAA
TATCCAGGTTATTCAACTCAAGGNGTTACAAAAACAAATAATGGTTATGAGGCAACACTT
AACCTTATTTCAGCAGGTCCATATGGTAACGATATANAACAATTAAATTTTCAATTAACT
TTTGAAACTAGTCAAATTTTTANAGTTANAATTACTGACCCAAATAATCAAAGATGGGAA
GTCCCACCAACTGTTAATCAATTAGTTGGANAAAATCCANATTCAACTGATTATATAATT
GAATTTACAAATAATCCATTTGGTTTTGCAGCAACTCGTATTTCAACTGGNGAAGTTTTA
TTTAATACAACTCAACCAAGTGATTGTTCATTTAATGGTTTAATTTATTCAAATTATTAT
TTANAATTAAGTNCATCATTCACAGAGAGTAATCCAAATATTTATGGTTTAGGXXXXXXX
XXXGTTGANTTTGGATTANATTAAAAATTANCNACNAANNAAATTAAANNTAGNTANATT
TTTTTNTAAAATAAAAAAAAAAAAAATAAAAAAAATAAATTATTTATTAAATTTTTA
sequence update 2002.10.25
Translated Amino Acid sequence 
svlki*TKTKKKMKLLSSLIIFFVIVLFXVVGSLSASLCKYPGYSTQGVTKTNNGYEATL
NLISAGPYGNDIXQLNFQLTFETSQIFXVXITDPNNQRWEVPPTVNQLVGXNPXSTDYII
EFTNNPFGFAATRISTGEVLFNTTQPSDCSFNGLIYSNYYLXLSXSFTESNPNIYGL---

---LXLDXIKNXXXXKLXXXXFFXKIKKKKIKKINYLLNF
Translated Amino Acid sequence (All Frames) 
Frame A:
svlki*TKTKKKMKLLSSLIIFFVIVLFXVVGSLSASLCKYPGYSTQGVTKTNNGYEATL
NLISAGPYGNDIXQLNFQLTFETSQIFXVXITDPNNQRWEVPPTVNQLVGXNPXSTDYII
EFTNNPFGFAATRISTGEVLFNTTQPSDCSFNGLIYSNYYLXLSXSFTESNPNIYGL---

---vxfglx*klxtxxikxxxifx*nkkkknkknklfikfl

Frame B:
vc*kyklklkkk*nfclh*lfflllyyfxlldhyqhhyvniqviqlkxlqkqimvmrqhl
tlfqqvhmvtixnn*ifn*llklvkflxlxlltqiikdgkshqllin*lxkixiqlii*l
nlqiihlvlqqlvfqlxkfyliqlnqvivhlmv*fiqiiixn*vhhsqrviqifmv*---

---LXLDXIKNXXXXKLXXXXFFXKIKKKKIKKINYLLNF

Frame C:
cvknin*n*kknetfvfinyffcyciilxcwiiisiim*isrlfnsrxyknk*wl*gnt*
pyfsrsiw*ryxtikfsinf*n*snfxsxny*pk*skmgsptnc*siswxksxfn*lyn*
iyk*siwfcsnsyfnwxsfi*ynstk*lfi*wfnlfkllfxikxiihre*skylwfr---

---*xwixlkixxxxn*x*xxffxk*kkkk*kk*iiy*if
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHG169 (SHG169Q) /CSM/SH/SHG1-C/SHG169Q.Seq.d/                        898   0.0
SHK543 (SHK543Q) /CSM/SH/SHK5-B/SHK543Q.Seq.d/                        890   0.0
SHK278 (SHK278Q) /CSM/SH/SHK2-D/SHK278Q.Seq.d/                        890   0.0
SHI850 (SHI850Q) /CSM/SH/SHI8-C/SHI850Q.Seq.d/                        890   0.0
SHI181 (SHI181Q) /CSM/SH/SHI1-D/SHI181Q.Seq.d/                        890   0.0
SHG509 (SHG509Q) /CSM/SH/SHG5-A/SHG509Q.Seq.d/                        890   0.0
SHA276 (SHA276Q) /CSM/SH/SHA2-D/SHA276Q.Seq.d/                        890   0.0
AHQ736 (AHQ736Q) /CSM/AH/AHQ7-B/AHQ736Q.Seq.d/                        886   0.0
SHG203 (SHG203Q) /CSM/SH/SHG2-A/SHG203Q.Seq.d/                        884   0.0
CHF189 (CHF189Q) /CSM/CH/CHF1-D/CHF189Q.Seq.d/                        884   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AE014839|AE014839.1 Plasmodium falciparum 3D7 chromosome 11 section 4 of 8 of the complete sequence.    36   0.021  9
AC116032|AC116032.2 Dictyostelium discoideum chromosome 2 map 4158743-4189373 strain AX4, complete sequence.    36   0.23   4
BX572075|BX572075.8 Zebrafish DNA sequence from clone CH211-158N13 in linkage group 12.    40   0.30   5
AC117072|AC117072.3 Dictyostelium discoideum chromosome 2 map 3879572-4071762 strain AX4, complete sequence.    34   0.38   7
AY573125|AY573125.1 Palloptera ambusta 16S ribosomal RNA gene, partial sequence; mitochondrial.    38   0.82   2
AL449924|AL449924.1 Streptococcus pneumoniae serotype 19F  *** SEQUENCING IN PROGRESS  *** from clone G54.    36   0.94   3
BH944911|BH944911.1 obu81h08.b1 B.oleracea002 Brassica oleracea genomic, DNA sequence.    46   1.00   1
AJ928065|AJ928065.1 Theileria annulata EST, clone tam011f04_p1k.    34   1.8    3
AE014822|AE014822.1 Plasmodium falciparum 3D7 chromosome 14 section 7 of 13 of the complete sequence.    32   1.9    2
AL606494|AL606494.4 Mouse DNA sequence from clone RP23-198B6 on chromosome 2.    40   2.0    4
dna update 2006. 3.26
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BX294013_13(BX294013|pid:none) Neurospora crassa DNA linkage gro...    72   1e-11
BC115034_1(BC115034|pid:none) Homo sapiens sucrase-isomaltase (a...    70   4e-11
BC116452_1(BC116452|pid:none) Homo sapiens sucrase-isomaltase (a...    70   4e-11
(P14410) RecName: Full=Sucrase-isomaltase, intestinal; Contains:...    70   4e-11
M22616_1(M22616|pid:none) Human sucrase-isomaltase mRNA, partial...    70   4e-11
AM920436_1367(AM920436|pid:none) Penicillium chrysogenum Wiscons...    68   2e-10
CU640366_6(CU640366|pid:none) Podospora anserina genomic DNA chr...    68   2e-10
A36690(A36690) sucrose alpha-glucosidase (EC 3.2.1.48) - rat (fr...    66   7e-10
(Q6P7A9) RecName: Full=Lysosomal alpha-glucosidase;          EC=...    66   1e-09
(P70699) RecName: Full=Lysosomal alpha-glucosidase;          EC=...    65   2e-09
protein update 2009. 4.18
PSORT 

 psg: 1.02  gvh: 0.72  alm: 0.49  top: 0.27  tms: 0.00  mit: 0.33  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: extracellular, including cell wall
	 12.0 %: endoplasmic reticulum
	  8.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: nuclear

>> prediction for SHG169 is exc
5' end seq. ID SHG169F
5' end seq. 
>SHG169F.Seq
AGTGTGTTAAAAATATAAACTAAAACTAAAAAAAAAATGAAACTTTTGTCTTCATTAATT
ATTTTTTTTGTTATTGTATTATTTTGNGTTGTTGGATCATTATCAGCATCATTATGTAAA
TATCCAGGTTATTCAACTCAAGGNGTTACAAAAACAAATAATGGTTATGAGGCAACACTT
AACCTTATTTCAGCAGGTCCATATGGTAACGATATANAACAATTAAATTTTCAATTAACT
TTTGAAACTAGTCAAATTTTTANAGTTANAATTACTGACCCAAATAATCAAAGATGGGAA
GTCCCACCAACTGTTAATCAATTAGTTGGANAAAATCCANATTCAACTGATTATATAATT
GAATTTACAAATAATCCATTTGGTTTTGCAGCAACTCGTATTTCAACTGGNGAAGTTTTA
TTTAATACAACTCAACCAAGTGATTGTTCATTTAATGGTTTAATTTATTCAAATTATTAT
TTANAATTAAGTNCATCATTCACAGAGAGTAATCCAAATATTTATGGTTTAGGNNNNNNN
NNN
Length of 5' end seq. 543
3' end seq. ID SHG169Z
3' end seq. 
>SHG169Z.Seq
NNNNNNNNNNGTTGANTTTGGATTANATTAAAAATTANCNACNAANNAAATTAAANNTAG
NTANATTTTTTTNTAAAATAAAAAAAAAAAAAATAAAAAAAATAAATTATTTATTAAATT
TTTA
Length of 3' end seq. 124
Connected seq. ID SHG169P
Connected seq. 
>SHG169P.Seq
AGTGTGTTAAAAATATAAACTAAAACTAAAAAAAAAATGAAACTTTTGTCTTCATTAATT
ATTTTTTTTGTTATTGTATTATTTTGNGTTGTTGGATCATTATCAGCATCATTATGTAAA
TATCCAGGTTATTCAACTCAAGGNGTTACAAAAACAAATAATGGTTATGAGGCAACACTT
AACCTTATTTCAGCAGGTCCATATGGTAACGATATANAACAATTAAATTTTCAATTAACT
TTTGAAACTAGTCAAATTTTTANAGTTANAATTACTGACCCAAATAATCAAAGATGGGAA
GTCCCACCAACTGTTAATCAATTAGTTGGANAAAATCCANATTCAACTGATTATATAATT
GAATTTACAAATAATCCATTTGGTTTTGCAGCAACTCGTATTTCAACTGGNGAAGTTTTA
TTTAATACAACTCAACCAAGTGATTGTTCATTTAATGGTTTAATTTATTCAAATTATTAT
TTANAATTAAGTNCATCATTCACAGAGAGTAATCCAAATATTTATGGTTTAGG-------
---GTTGANTTTGGATTANATTAAAAATTANCNACNAANNAAATTAAANNTAGNTANATT
TTTTTNTAAAATAAAAAAAAAAAAAATAAAAAAAATAAATTATTTATTAAATTTTTA
Length of connected seq. 647
Full length Seq ID -
Full length Seq. -
Length of full length seq. -