>SHG125 (SHG125Q) /CSM/SH/SHG1-B/SHG125Q.Seq.d/
TGCTCAAAGTAAACGTGTTTTGGTAAACACTTGTAATATCACGCTTTCATCGATCCATCC
AAATTATGGTCCAACTTCTGGTGGTTCATTGGTATCTATTAGTGGTTCTGGTTTTAATTG
TGGCATTAGTAATGTATTGTTTGGTGGTGCTGCATGCTCAAATCCAAAGGTTAAAACAGA
TGGACTCTTAACTTGTACAACTCCTTCCTCTGATAATAATGGTCCAGCTGATATTGTTGT
CAAAGGTTCTGATGGTAGTACCGTTACATTAACTGAAGCCTATAACTATGTTTCAACCAT
TGCAATTACTTCATGTGCACTCGGTGCAAACAATGGTAATTTCTTTAGTTGTGATATCTC
TTCTCCAGTTGATACTTCAAATAGGCCTTCATGTAGTTCTCTTTTCTCTTCTGCAACTTT
ACAAACTTTGGGTAGTTCTCCAAGATGTGAATATACTGCTTCAAACATTCTAATGGTTAC
ATTGGGTAGTGGTGAAAGTGTATCAGTTGGCCAACAATTGGAATTTTCTCAAGGTGGAAT
CACXXXXXXXXXXGGCAGATGGTATTCTTCAAAGAACTGCAATTGGTGAAGAACCATTTA
CAGTTGCAAGTGATAAAGGTTTCTCGATTACAACATTTAAAACTCTACATATGGAATTAA
GTAAGAGTGGTCAATCGATTGGTTTCAAAAAGGATGATGAAGGTCAATCTCTTGATGGTG
AAAAACTTGCTTTCACTCCAAAATTCAGTATTCCATCAAGTGTTTTATCAAAGAATAAAT
TAAAACAAACCTCTGAAATCACTGGTAAACTAATTACAGTTCCATCAAATATTCATTTCA
ATCATAAATCTGATCAAACCATTTTCCCAAAAACTGTTTCACTTGAACTTTTCAATGGTG
GCTCTCAATTATCAATCAATGGCCTTAAAGATCAAATTATCATTGATATGGGAAAACATT
CACCACCAAAAGATAAAGAATTTAAATGTGGTTGGTGGAGCTCCAATAAAAAAGAATGGT
TAACAGATGGTTGTTCAACCCATAAAGTAGATGGTAATATTCAATGTAAATGTAACCATC
TTACAGAGTTTGGTTTAATTGAATTTGCAAAATCCTCTCAAAACATTTGGATCATTATTG
GACCAGTAGTTGGTGGTGTTGTATTTGCAGCTGCAATGGTTGGTACTGTTGTATTGGTAA
AGAGAAAGAAAAACAAAAACAAAAAGAACAAATCAAAGAAAACCAAAGAACAAATCAACA
AAGAAAAAGAAGACAAAAAGAAACAAGCAAAAGAAAAGAAAGAAGC

AQSKRVLVNTCNITLSSIHPNYGPTSGGSLVSISGSGFNCGISNVLFGGAACSNPKVKTD
GLLTCTTPSSDNNGPADIVVKGSDGSTVTLTEAYNYVSTIAITSCALGANNGNFFSCDIS
SPVDTSNRPSCSSLFSSATLQTLGSSPRCEYTASNILMVTLGSGESVSVGQQLEFSQGGI
---

---ADGILQRTAIGEEPFTVASDKGFSITTFKTLHMELSKSGQSIGFKKDDEGQSLDGEK
LAFTPKFSIPSSVLSKNKLKQTSEITGKLITVPSNIHFNHKSDQTIFPKTVSLELFNGGS
QLSINGLKDQIIIDMGKHSPPKDKEFKCGWWSSNKKEWLTDGCSTHKVDGNIQCKCNHLT
EFGLIEFAKSSQNIWIIIGPVVGGVVFAAAMVGTVVLVKRKKNKNKKNKSKKTKEQINKE
KEDKKKQAKEKKE

Frame A:
csk*tcfgkhl*yhafidpsklwsnfwwfigiy*wfwf*lwh**civwwccmlkskg*nr
wtlnlynsfl***wss*yccqrf*w*yryin*sl*lcfnhcnyfmctrckqw*fl*l*yl
fss*yfk*afm*fsflfcnftnfg*fskm*iycfkhsngyig*w*kciswptigifsrwn
h---

---grwysskncnw*rtiysck**rfldyni*nstygik*ewsidwfqkg**rsis*w*k
tcfhskiqysikcfike*iktnl*nhw*tnyssikysfqs*i*snhfpkncft*tfqwwl
siinqwp*rsnyh*ygktfttkr*ri*mwlvelq*krmvnrwlfnp*srw*ysm*m*psy
rvwfn*ickilskhldhywtsswwccicscngwyccigkekekqkqkeqikenqrtnqqr
krrqketskrkers

Frame B:
AQSKRVLVNTCNITLSSIHPNYGPTSGGSLVSISGSGFNCGISNVLFGGAACSNPKVKTD
GLLTCTTPSSDNNGPADIVVKGSDGSTVTLTEAYNYVSTIAITSCALGANNGNFFSCDIS
SPVDTSNRPSCSSLFSSATLQTLGSSPRCEYTASNILMVTLGSGESVSVGQQLEFSQGGI
---

---ADGILQRTAIGEEPFTVASDKGFSITTFKTLHMELSKSGQSIGFKKDDEGQSLDGEK
LAFTPKFSIPSSVLSKNKLKQTSEITGKLITVPSNIHFNHKSDQTIFPKTVSLELFNGGS
QLSINGLKDQIIIDMGKHSPPKDKEFKCGWWSSNKKEWLTDGCSTHKVDGNIQCKCNHLT
EFGLIEFAKSSQNIWIIIGPVVGGVVFAAAMVGTVVLVKRKKNKNKKNKSKKTKEQINKE
KEDKKKQAKEKKE

Frame C:
lkvnvfw*tlvisrfhrsiqimvqllvvhwyllvvlvlivalvmyclvvlhaqiqrlkqm
ds*lvqllpliimvqlillskvlmvvplh*lkpitmfqplqllhvhsvqtmvislvvisl
lqlilqiglhvvlfsllqlyklwvvlqdvnillqtf*wlhwvvvkvyqlannwnflkves
---

---qmvffkelqlvknhlqlqvikvsrlqhlklyiwn*vrvvnrlvskrmmkvnllmvkn
llslqnsvfhqvfyqrin*nkplkslvn*lqfhqifisiinlikpfsqklfhlnfsmval
nyqsmalkiklsliwenihhqkiknlnvvggapikkng*qmvvqpik*mvifnvnvtilq
slv*lnlqnplktfgslldq*lvvlylqlqwlvllyw*rerktktkrtnqrkpknkstkk
kktkrnkqkkrkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHG125 (SHG125Q) /CSM/SH/SHG1-B/SHG125Q.Seq.d/                       2315   0.0
SHL489 (SHL489Q) /CSM/SH/SHL4-D/SHL489Q.Seq.d/                       1279   0.0
SHL119 (SHL119Q) /CSM/SH/SHL1-A/SHL119Q.Seq.d/                       1279   0.0
SHK593 (SHK593Q) /CSM/SH/SHK5-D/SHK593Q.Seq.d/                       1279   0.0
SHJ824 (SHJ824Q) /CSM/SH/SHJ8-A/SHJ824Q.Seq.d/                       1279   0.0
SHD762 (SHD762Q) /CSM/SH/SHD7-C/SHD762Q.Seq.d/                       1279   0.0
SHD428 (SHD428Q) /CSM/SH/SHD4-B/SHD428Q.Seq.d/                       1279   0.0
SHD253 (SHD253Q) /CSM/SH/SHD2-C/SHD253Q.Seq.d/                       1279   0.0
SHB414 (SHB414Q) /CSM/SH/SHB4-A/SHB414Q.Seq.d/                       1279   0.0
SFG748 (SFG748Q) /CSM/SF/SFG7-B/SFG748Q.Seq.d/                       1279   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC024453|AC024453.2 Homo sapiens chromosome 4 clone RP11-2B17 map 4, WORKING DRAFT SEQUENCE, 51 unordered pieces.    48   0.51   1
AC013774|AC013774.3 Homo sapiens clone RP11-10P1, WORKING DRAFT SEQUENCE, 11 unordered pieces.    48   0.51   1
AC103541|AC103541.6 Homo sapiens BAC clone RP11-818M13 from 4, complete sequence.    48   0.51   1
AC097518|AC097518.2 Homo sapiens BAC clone RP11-487E13 from 4, complete sequence.    48   0.51   1
AJ410884|AJ410884.1 Influenza A virus (swine/Cotes d'Armor/1515/99 (H1N1)) partial N1NA  gene for Neuraminidase, genomic RNA.    42   0.94   2
AJ410883|AJ410883.1 Influenza A virus (swine/Cotes d'Armor/1482/99 (H1N1)) partial N1NA  gene for Neuraminidase, genomic RNA.    42   0.97   2
AC180621|AC180621.1 Strongylocentrotus purpuratus clone R3-3092G22, WORKING DRAFT SEQUENCE, 24 unordered pieces.    46   2.0    1
BI347313|BI347313.1 fbn-ek036 Cattle pUC18 mammary gland library Bos taurus cDNA, mRNA sequence.    46   2.0    1
AQ290526|AQ290526.1 nbxb0037A02r CUGI Rice BAC Library Oryza sativa genomic clone nbxb0037A02r, genomic survey sequence.    46   2.0    1
CZ857443|CZ857443.1 OC__Ba0249B12.r OC__Ba Oryza coarctata genomic clone OC__Ba0249B12 3', genomic survey sequence.    46   2.0    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK154648_1(AK154648|pid:none) Mus musculus NOD-derived CD11c +ve...    50   2e-04
(Q8R0T6) RecName: Full=Probable G-protein coupled receptor 97; F...    50   2e-04
AK037149_1(AK037149|pid:none) Mus musculus 6 days neonate skin c...    50   2e-04
AB041939_1(AB041939|pid:none) Homo sapiens KPG_006 mRNA for G-pr...    50   2e-04
AF216967_1(AF216967|pid:none) Homo sapiens putative vascular ind...    48   6e-04
(Q8K3V3) RecName: Full=G-protein coupled receptor 56; Flags: Pre...    47   0.002
AK295345_1(AK295345|pid:none) Homo sapiens cDNA FLJ55998 complet...    46   0.003
AF166382_1(AF166382|pid:none) Mus musculus serpentine receptor (...    45   0.004
AY181246_1(AY181246|pid:none) Homo sapiens G protein-coupled rec...    45   0.007
(Q96K78) RecName: Full=Probable G-protein coupled receptor 128; ...    45   0.007

 psg: 0.84  gvh: 0.50  alm: 0.22  top: 1.17  tms: 0.07  mit: 0.36  mip: 0.02
 nuc: 0.17  erl: 0.00  erm: 1.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.01  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 1.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 16.0 %: nuclear
	 12.0 %: mitochondrial
	 12.0 %: vesicles of secretory system
	  8.0 %: Golgi
	  8.0 %: endoplasmic reticulum
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for SHG125 is cyt

>SHG125F.Seq
TGCTCAAAGTAAACGTGTTTTGGTAAACACTTGTAATATCACGCTTTCATCGATCCATCC
AAATTATGGTCCAACTTCTGGTGGTTCATTGGTATCTATTAGTGGTTCTGGTTTTAATTG
TGGCATTAGTAATGTATTGTTTGGTGGTGCTGCATGCTCAAATCCAAAGGTTAAAACAGA
TGGACTCTTAACTTGTACAACTCCTTCCTCTGATAATAATGGTCCAGCTGATATTGTTGT
CAAAGGTTCTGATGGTAGTACCGTTACATTAACTGAAGCCTATAACTATGTTTCAACCAT
TGCAATTACTTCATGTGCACTCGGTGCAAACAATGGTAATTTCTTTAGTTGTGATATCTC
TTCTCCAGTTGATACTTCAAATAGGCCTTCATGTAGTTCTCTTTTCTCTTCTGCAACTTT
ACAAACTTTGGGTAGTTCTCCAAGATGTGAATATACTGCTTCAAACATTCTAATGGTTAC
ATTGGGTAGTGGTGAAAGTGTATCAGTTGGCCAACAATTGGAATTTTCTCAAGGTGGAAT
CACNNNNNNNNNN

>SHG125Z.Seq
NNNNNNNNNNGGCAGATGGTATTCTTCAAAGAACTGCAATTGGTGAAGAACCATTTACAG
TTGCAAGTGATAAAGGTTTCTCGATTACAACATTTAAAACTCTACATATGGAATTAAGTA
AGAGTGGTCAATCGATTGGTTTCAAAAAGGATGATGAAGGTCAATCTCTTGATGGTGAAA
AACTTGCTTTCACTCCAAAATTCAGTATTCCATCAAGTGTTTTATCAAAGAATAAATTAA
AACAAACCTCTGAAATCACTGGTAAACTAATTACAGTTCCATCAAATATTCATTTCAATC
ATAAATCTGATCAAACCATTTTCCCAAAAACTGTTTCACTTGAACTTTTCAATGGTGGCT
CTCAATTATCAATCAATGGCCTTAAAGATCAAATTATCATTGATATGGGAAAACATTCAC
CACCAAAAGATAAAGAATTTAAATGTGGTTGGTGGAGCTCCAATAAAAAAGAATGGTTAA
CAGATGGTTGTTCAACCCATAAAGTAGATGGTAATATTCAATGTAAATGTAACCATCTTA
CAGAGTTTGGTTTAATTGAATTTGCAAAATCCTCTCAAAACATTTGGATCATTATTGGAC
CAGTAGTTGGTGGTGTTGTATTTGCAGCTGCAATGGTTGGTACTGTTGTATTGGTAAAGA
GAAAGAAAAACAAAAACAAAAAGAACAAATCAAAGAAAACCAAAGAACAAATCAACAAAG
AAAAAGAAGACAAAAAGAAACAAGCAAAAGAAAAGAAAGAAGC

>SHG125P.Seq
TGCTCAAAGTAAACGTGTTTTGGTAAACACTTGTAATATCACGCTTTCATCGATCCATCC
AAATTATGGTCCAACTTCTGGTGGTTCATTGGTATCTATTAGTGGTTCTGGTTTTAATTG
TGGCATTAGTAATGTATTGTTTGGTGGTGCTGCATGCTCAAATCCAAAGGTTAAAACAGA
TGGACTCTTAACTTGTACAACTCCTTCCTCTGATAATAATGGTCCAGCTGATATTGTTGT
CAAAGGTTCTGATGGTAGTACCGTTACATTAACTGAAGCCTATAACTATGTTTCAACCAT
TGCAATTACTTCATGTGCACTCGGTGCAAACAATGGTAATTTCTTTAGTTGTGATATCTC
TTCTCCAGTTGATACTTCAAATAGGCCTTCATGTAGTTCTCTTTTCTCTTCTGCAACTTT
ACAAACTTTGGGTAGTTCTCCAAGATGTGAATATACTGCTTCAAACATTCTAATGGTTAC
ATTGGGTAGTGGTGAAAGTGTATCAGTTGGCCAACAATTGGAATTTTCTCAAGGTGGAAT
CAC----------GGCAGATGGTATTCTTCAAAGAACTGCAATTGGTGAAGAACCATTTA
CAGTTGCAAGTGATAAAGGTTTCTCGATTACAACATTTAAAACTCTACATATGGAATTAA
GTAAGAGTGGTCAATCGATTGGTTTCAAAAAGGATGATGAAGGTCAATCTCTTGATGGTG
AAAAACTTGCTTTCACTCCAAAATTCAGTATTCCATCAAGTGTTTTATCAAAGAATAAAT
TAAAACAAACCTCTGAAATCACTGGTAAACTAATTACAGTTCCATCAAATATTCATTTCA
ATCATAAATCTGATCAAACCATTTTCCCAAAAACTGTTTCACTTGAACTTTTCAATGGTG
GCTCTCAATTATCAATCAATGGCCTTAAAGATCAAATTATCATTGATATGGGAAAACATT
CACCACCAAAAGATAAAGAATTTAAATGTGGTTGGTGGAGCTCCAATAAAAAAGAATGGT
TAACAGATGGTTGTTCAACCCATAAAGTAGATGGTAATATTCAATGTAAATGTAACCATC
TTACAGAGTTTGGTTTAATTGAATTTGCAAAATCCTCTCAAAACATTTGGATCATTATTG
GACCAGTAGTTGGTGGTGTTGTATTTGCAGCTGCAATGGTTGGTACTGTTGTATTGGTAA
AGAGAAAGAAAAACAAAAACAAAAAGAACAAATCAAAGAAAACCAAAGAACAAATCAACA
AAGAAAAAGAAGACAAAAAGAAACAAGCAAAAGAAAAGAAAGAAGC