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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHF852
Library SH
(Link to library)
Clone ID SHF852
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11129-1
Original site URL
Representative seq. ID SHF852P
(Link to Original site)
Representative DNA sequence 
>SHF852 (SHF852Q) /CSM/SH/SHF8-C/SHF852Q.Seq.d/
GATCAACATGTTCAACCTGTCAAAGAGGTATTAATAAAGAAGCAGTTCGTATTGGTTATA
AAACAAAATCAAAACACTTTGATGGAATGGATGTATCATGGCATCATTTAAAATGTAAAT
GTCCACAAGTACCATCATTTACAGATTTAATTCACTGGGAATACCTTCGTTGGGAAGACC
AATTATCAATTAAAACAACTTATTTTTCATCTGAAAAGTATGATCCAAAATCAGCATCAG
AGGTACAAAGAGAGAAATATTTAAAGGCATTATGGGAAGTTAAAGATAGTATTGCCGATA
ACTTGAAGGGACCAGCAATTAAAAACATCATTCAATATAATAAAGGTTATGTTGATAAGG
TATCACCAGTTCATTTACTTCATACTCTCTCTGATTGGATGTTAAAAGGTCGTCCAGGTA
GATGTCCAACTTGTAAGAATTTCGATATACTTTTCAATGGTATCGAATATCAATGTAAAG
GTTGGATTTCAGGTTTCACAAAATGTGATTGGAAAGGTGATTCAATTGAAAGATGGGCTG
TTCAATTTCCTGATGATCTTTCAAAAAATAAATTCCTTTCAACTTTTAAATATTCXXXXX
XXXXXCTTAAGAAGCAAACTGAATCCACTCGAGGGTAACATGATTGAAAATCAATATATT
ACACTCAAAAACCAAACTCACTCCACTTGATAAAGATAGTGTAGTTTATAAAACNTTGGT
AGATTATGTTGCCAATAGTCAAGATAAACAATTTAGAACCAATATCTGTGTTCAAGATAT
TCTTTCACTTGAGAGAGAGAATGAAAGTGAACGTTTCACTCCTTGGGCAAAAGATAAGAA
TAGATTACTCTTATGGCATGGTAGTAGGTTAACTAATTTCGTATCGATTGTCTCGCAGGG
ATTAAGAATTGCTCCACCATCTGCTCCAAAAACTGGTTACAGATTTGGTAAAGGTATTTA
TTTTGCTGATTGTATTAGTAAATCATTCAGCTATTGTTTCACATCAAGTGATTGTCCAAC
TGCACTTATGTTATTATGTGAAGTTTCACTTGGTGACATGAATGAATTGAAACATGATAC
CTATATGGAAGAGGCACCACATCCATTCCATAGTACAAAAGCTTTAGGTATGGCTGCTCC
TCACAAAGATGGTAATCATCCTCTTTCTGATTCTGATGGTTTAGTAGTACCATTAGGTAA
AATTTCAAAGACTGGTCTCTCAACTTCATGTACTCACAATGAATTCATTGTCTATAAAAT
TGAACAAGTCAGAATCAAATACATCTTAAGAGTTTACAAGGAAAATAAAATAAAATAAAA
TTTTTTAAAATTATCCT
sequence update 2002.10.25
Translated Amino Acid sequence 
STCSTCQRGINKEAVRIGYKTKSKHFDGMDVSWHHLKCKCPQVPSFTDLIHWEYLRWEDQ
LSIKTTYFSSEKYDPKSASEVQREKYLKALWEVKDSIADNLKGPAIKNIIQYNKGYVDKV
SPVHLLHTLSDWMLKGRPGRCPTCKNFDILFNGIEYQCKGWISGFTKCDWKGDSIERWAV
QFPDDLSKNKFLSTFKY---

---LRSKLNPLEGNMIENQYITLKNQTHST**r*csl*nxgrlccq*sr*ti*nqylcsr
ysft*ere*k*tfhslgkr*e*itlmaw**vn*fridclagikncsticsknwlqiw*ry
lfc*ly**iiqllfhik*lsnctyvim*sftw*he*iet*ylygrgttsip*yksfrygc
ssqrw*sssf*f*wfsstir*nfkdwslnfmysq*ihcl*n*tsqnqihlkslqgk*nki
kffkii
Translated Amino Acid sequence (All Frames) 
Frame A:
dqhvqpvkevlikkqfvlvikqnqntlmewmyhgii*nvnvhkyhhlqi*ftgntfvgkt
nyqlkqlifhlksmiqnqhqrykerni*rhygklkivlpit*rdqqlktsfniikvmlir
yhqfiyfilsligc*kvvqvdvqlvrisiyfsmvsninvkvgfqvsqnvigkviqlkdgl
fnflmifqkinsfqllni---

---lkkqtestrg*hd*ksiyytqkpnslhlikiv*fikxw*imlpivkinnlepisvfk
iffhlrermkvnvsllgqkiridysygmvvg*lisyrlsrrd*ellhhllqklvtdlvkv
fillivlvnhsaivshqvivqlhlcyyvkfhlvt*mn*nmipiwkrhhihsivqkl*vwl
lltkmviilflilmv**yh*vkfqrlvsqlhvltmnslsiklnksesnts*eftrkik*n
kif*nyp

Frame B:
inmfnlskry**rsssywl*nkiktl*wngcimasfkm*mststiiyrfnslgipslgrp
iin*nnlffi*kv*skisirgtkreifkgimgs*r*ycr*legtsn*khhsi**rlc**g
itssftsysl*ldvkrssr*msnl*efrytfqwyrism*rldfrfhkm*ler*fn*kmgc
sis**sfkk*ipfnf*if---

---LRSKLNPLEGNMIENQYITLKNQTHST**r*csl*nxgrlccq*sr*ti*nqylcsr
ysft*ere*k*tfhslgkr*e*itlmaw**vn*fridclagikncsticsknwlqiw*ry
lfc*ly**iiqllfhik*lsnctyvim*sftw*he*iet*ylygrgttsip*yksfrygc
ssqrw*sssf*f*wfsstir*nfkdwslnfmysq*ihcl*n*tsqnqihlkslqgk*nki
kffkii

Frame C:
STCSTCQRGINKEAVRIGYKTKSKHFDGMDVSWHHLKCKCPQVPSFTDLIHWEYLRWEDQ
LSIKTTYFSSEKYDPKSASEVQREKYLKALWEVKDSIADNLKGPAIKNIIQYNKGYVDKV
SPVHLLHTLSDWMLKGRPGRCPTCKNFDILFNGIEYQCKGWISGFTKCDWKGDSIERWAV
QFPDDLSKNKFLSTFKY---

---*ean*ihsrvt*lkinilhsktkltpldkdsvvyktlvdyvansqdkqfrtnicvqd
ilslereneserftpwakdknrlllwhgsrltnfvsivsqglriappsapktgyrfgkgi
yfadcisksfsycftssdcptalmllcevslgdmnelkhdtymeeaphpfhstkalgmaa
phkdgnhplsdsdglvvplgkisktglstscthnefivykieqvrikyilrvykenkik*
nflkls
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHF852 (SHF852Q) /CSM/SH/SHF8-C/SHF852Q.Seq.d/                       2512   0.0
SHJ664 (SHJ664Q) /CSM/SH/SHJ6-C/SHJ664Q.Seq.d/                       1356   0.0
CHP243 (CHP243Q) /CSM/CH/CHP2-B/CHP243Q.Seq.d/                       1356   0.0
AHM188 (AHM188Q) /CSM/AH/AHM1-D/AHM188Q.Seq.d/                       1356   0.0
AHH466 (AHH466Q) /CSM/AH/AHH4-C/AHH466Q.Seq.d/                       1346   0.0
CHQ835 (CHQ835Q) /CSM/CH/CHQ8-B/CHQ835Q.Seq.d/                       1342   0.0
VHB869 (VHB869Q) /CSM/VH/VHB8-C/VHB869Q.Seq.d/                       1340   0.0
VHA783 (VHA783Q) /CSM/VH/VHA7-D/VHA783Q.Seq.d/                       1340   0.0
SHD815 (SHD815Q) /CSM/SH/SHD8-A/SHD815Q.Seq.d/                       1340   0.0
VHD227 (VHD227Q) /CSM/VH/VHD2-B/VHD227Q.Seq.d/                       1334   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AJ534948|AJ534948.1 Dictyostelium discoideum mRNA for NAD(+) ADP-ribosyltransferase-1A (adprt-1A gene).  1239   0.0    3
AJ520099|AJ520099.1 Dictyostelium discoideum mRNA for (ADP-ribosyl)transferase (adprt2  gene).    62   3e-14  3
DY229813|DY229813.1 ID0AAK8YC07CM1 ApHL3SD Acyrthosiphon pisum cDNA clone ID0AAK8YC07 5', mRNA sequence.    60   1e-04  1
AM113503|AM113503.1 Streptococcus sp. 2410 lytA gene for N-acetylmuramoyl-L-alanine  amidase, strain 2410.    50   0.13   1
AM113502|AM113502.1 Streptococcus sp. 3137 lytA gene for N-acetylmuramoyl-L-alanine  amidase, strain 3137.    50   0.13   1
AM113501|AM113501.1 Streptococcus sp. 1956 lytA gene for N-acetylmuramoyl-L-alanine  amidase, strain 1956.    50   0.13   1
AM113500|AM113500.1 Streptococcus sp. 1504 lytA gene for N-acetylmuramoyl-L-alanine  amidase, strain 1504.    50   0.13   1
AM113497|AM113497.1 Streptococcus sp. 578 lytA gene for N-acetylmuramoyl-L-alanine  amidase, strain 578.    50   0.13   1
AM113496|AM113496.1 Streptococcus pseudopneumoniae lytA gene for  N-acetylmuramoyl-L-alanine amidase, strain CCUG 48465.    50   0.13   1
AM113495|AM113495.1 Streptococcus pseudopneumoniae lytA gene for  N-acetylmuramoyl-L-alanine amidase, type strain CCUG 49455.    50   0.13   1
dna update 2006. 3.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

X65497_1(X65497|pid:none) R.norvegicus mRNA for poly(ADP-ribose)...   189   2e-46
(P27008) RecName: Full=Poly [ADP-ribose] polymerase 1;          ...   189   2e-46
PDBF(1A26) MOL_ID: 1; MOLECULE: POLY (ADP-RIBOSE) POLYMERASE; CH...   189   2e-46
(P26446) RecName: Full=Poly [ADP-ribose] polymerase 1;          ...   189   2e-46
AK303340_1(AK303340|pid:none) Homo sapiens cDNA FLJ53442 complet...   187   5e-46
(Q9R152) RecName: Full=Poly [ADP-ribose] polymerase 1;          ...   187   7e-46
AK312339_1(AK312339|pid:none) Homo sapiens cDNA, FLJ92658, highl...   187   9e-46
J03473_1(J03473|pid:none) Human poly(ADP-ribose) synthetase mRNA...   187   9e-46
AB384394_1(AB384394|pid:none) Synthetic construct DNA, clone: pF...   187   9e-46
M17081_1(M17081|pid:none) Human placental poly(ADP-ribose) polym...   187   9e-46
protein update 2009. 4.18
PSORT 

 psg: 0.49  gvh: 0.38  alm: 0.54  top: 0.53  tms: 0.00  mit: 0.32  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 28.0 %: mitochondrial
	 24.0 %: cytoplasmic
	  8.0 %: vesicles of secretory system
	  4.0 %: cytoskeletal

>> prediction for SHF852 is nuc
5' end seq. ID SHF852F
5' end seq. 
>SHF852F.Seq
GATCAACATGTTCAACCTGTCAAAGAGGTATTAATAAAGAAGCAGTTCGTATTGGTTATA
AAACAAAATCAAAACACTTTGATGGAATGGATGTATCATGGCATCATTTAAAATGTAAAT
GTCCACAAGTACCATCATTTACAGATTTAATTCACTGGGAATACCTTCGTTGGGAAGACC
AATTATCAATTAAAACAACTTATTTTTCATCTGAAAAGTATGATCCAAAATCAGCATCAG
AGGTACAAAGAGAGAAATATTTAAAGGCATTATGGGAAGTTAAAGATAGTATTGCCGATA
ACTTGAAGGGACCAGCAATTAAAAACATCATTCAATATAATAAAGGTTATGTTGATAAGG
TATCACCAGTTCATTTACTTCATACTCTCTCTGATTGGATGTTAAAAGGTCGTCCAGGTA
GATGTCCAACTTGTAAGAATTTCGATATACTTTTCAATGGTATCGAATATCAATGTAAAG
GTTGGATTTCAGGTTTCACAAAATGTGATTGGAAAGGTGATTCAATTGAAAGATGGGCTG
TTCAATTTCCTGATGATCTTTCAAAAAATAAATTCCTTTCAACTTTTAAATATTCNNNNN
NNNNN
Length of 5' end seq. 605
3' end seq. ID SHF852Z
3' end seq. 
>SHF852Z.Seq
NNNNNNNNNNCTTAAGAAGCAAACTGAATCCACTCGAGGGTAACATGATTGAAAATCAAT
ATATTACACTCAAAAACCAAACTCACTCCACTTGATAAAGATAGTGTAGTTTATAAAACN
TTGGTAGATTATGTTGCCAATAGTCAAGATAAACAATTTAGAACCAATATCTGTGTTCAA
GATATTCTTTCACTTGAGAGAGAGAATGAAAGTGAACGTTTCACTCCTTGGGCAAAAGAT
AAGAATAGATTACTCTTATGGCATGGTAGTAGGTTAACTAATTTCGTATCGATTGTCTCG
CAGGGATTAAGAATTGCTCCACCATCTGCTCCAAAAACTGGTTACAGATTTGGTAAAGGT
ATTTATTTTGCTGATTGTATTAGTAAATCATTCAGCTATTGTTTCACATCAAGTGATTGT
CCAACTGCACTTATGTTATTATGTGAAGTTTCACTTGGTGACATGAATGAATTGAAACAT
GATACCTATATGGAAGAGGCACCACATCCATTCCATAGTACAAAAGCTTTAGGTATGGCT
GCTCCTCACAAAGATGGTAATCATCCTCTTTCTGATTCTGATGGTTTAGTAGTACCATTA
GGTAAAATTTCAAAGACTGGTCTCTCAACTTCATGTACTCACAATGAATTCATTGTCTAT
AAAATTGAACAAGTCAGAATCAAATACATCTTAAGAGTTTACAAGGAAAATAAAATAAAA
TAAAATTTTTTAAAATTATCCT
Length of 3' end seq. 742
Connected seq. ID SHF852P
Connected seq. 
>SHF852P.Seq
GATCAACATGTTCAACCTGTCAAAGAGGTATTAATAAAGAAGCAGTTCGTATTGGTTATA
AAACAAAATCAAAACACTTTGATGGAATGGATGTATCATGGCATCATTTAAAATGTAAAT
GTCCACAAGTACCATCATTTACAGATTTAATTCACTGGGAATACCTTCGTTGGGAAGACC
AATTATCAATTAAAACAACTTATTTTTCATCTGAAAAGTATGATCCAAAATCAGCATCAG
AGGTACAAAGAGAGAAATATTTAAAGGCATTATGGGAAGTTAAAGATAGTATTGCCGATA
ACTTGAAGGGACCAGCAATTAAAAACATCATTCAATATAATAAAGGTTATGTTGATAAGG
TATCACCAGTTCATTTACTTCATACTCTCTCTGATTGGATGTTAAAAGGTCGTCCAGGTA
GATGTCCAACTTGTAAGAATTTCGATATACTTTTCAATGGTATCGAATATCAATGTAAAG
GTTGGATTTCAGGTTTCACAAAATGTGATTGGAAAGGTGATTCAATTGAAAGATGGGCTG
TTCAATTTCCTGATGATCTTTCAAAAAATAAATTCCTTTCAACTTTTAAATATTC-----
-----CTTAAGAAGCAAACTGAATCCACTCGAGGGTAACATGATTGAAAATCAATATATT
ACACTCAAAAACCAAACTCACTCCACTTGATAAAGATAGTGTAGTTTATAAAACNTTGGT
AGATTATGTTGCCAATAGTCAAGATAAACAATTTAGAACCAATATCTGTGTTCAAGATAT
TCTTTCACTTGAGAGAGAGAATGAAAGTGAACGTTTCACTCCTTGGGCAAAAGATAAGAA
TAGATTACTCTTATGGCATGGTAGTAGGTTAACTAATTTCGTATCGATTGTCTCGCAGGG
ATTAAGAATTGCTCCACCATCTGCTCCAAAAACTGGTTACAGATTTGGTAAAGGTATTTA
TTTTGCTGATTGTATTAGTAAATCATTCAGCTATTGTTTCACATCAAGTGATTGTCCAAC
TGCACTTATGTTATTATGTGAAGTTTCACTTGGTGACATGAATGAATTGAAACATGATAC
CTATATGGAAGAGGCACCACATCCATTCCATAGTACAAAAGCTTTAGGTATGGCTGCTCC
TCACAAAGATGGTAATCATCCTCTTTCTGATTCTGATGGTTTAGTAGTACCATTAGGTAA
AATTTCAAAGACTGGTCTCTCAACTTCATGTACTCACAATGAATTCATTGTCTATAAAAT
TGAACAAGTCAGAATCAAATACATCTTAAGAGTTTACAAGGAAAATAAAATAAAATAAAA
TTTTTTAAAATTATCCT
Length of connected seq. 1327
Full length Seq ID -
Full length Seq. -
Length of full length seq. -