>SHF830 (SHF830Q) /CSM/SH/SHF8-B/SHF830Q.Seq.d/
TTTTAATGAAGTTTGCTATTCATTAAAAGATGGTGAAATTAAACTTCATTTAATTCCACA
TTCACATTGTGATTCTGGATGGACATCAACAATGAACGAATATTATATGGGTCAAGTTAA
AAGTATTATATCATCAATGGTTCAATCATTAAATGTTGAATCAAATCCACCTAGAAAATT
TGTTTGGTCTGAAATTGGATTTTTAGAACAATGGTGGGATGATATGCCAATTGAAATTAA
AAATGATTTTATAAAACATGTTAAAAATGATAGGATTGAATTTGTAAATGGTGGATGGGT
AATGAATGATGAAGCATGTGCAAGTTTAGAAAGTGTAATTAGACAATTATCAAATGGGCA
TAAATTTATTAGAGAGAAATTTGGAAAACAACCAGAGAGTGGATGGCAAATTGATCCATT
TGGACATTCATCATTGACACCAACATTACAAGCTCAATTTGGTTATAAACATGTTGTATT
GAATCGTATTCACTATGAATTGAAAAAGAAATTTAAAGAAGAAAAGAATTTACAATTTAA
ATGGAGAGXXXXXXXXXX

FNEVCYSLKDGEIKLHLIPHSHCDSGWTSTMNEYYMGQVKSIISSMVQSLNVESNPPRKF
VWSEIGFLEQWWDDMPIEIKNDFIKHVKNDRIEFVNGGWVMNDEACASLESVIRQLSNGH
KFIREKFGKQPESGWQIDPFGHSSLTPTLQAQFGYKHVVLNRIHYELKKKFKEEKNLQFK
WR---

Frame A:
f**sllfikrw*n*tsfnstftl*fwmdinnerilygss*kyyiingsiikc*ikst*ki
clv*nwifrtmvg*yan*n*k*fyktc*k**d*ickwwmgne**smckfrkcn*tiikwa
*iy*reiwkttrewman*siwtfiidtnitssiwl*tcciesysl*iekei*rrkefti*
me---


Frame B:
FNEVCYSLKDGEIKLHLIPHSHCDSGWTSTMNEYYMGQVKSIISSMVQSLNVESNPPRKF
VWSEIGFLEQWWDDMPIEIKNDFIKHVKNDRIEFVNGGWVMNDEACASLESVIRQLSNGH
KFIREKFGKQPESGWQIDPFGHSSLTPTLQAQFGYKHVVLNRIHYELKKKFKEEKNLQFK
WR---


Frame C:
lmkfaih*kmvklnfi*fhihivildghqq*tniiwvklkvlyhqwfnh*mlnqihlenl
fglkldf*nnggmicqlklkmil*nmlkmiglnl*mvdg**mmkhvqv*kv*ldnyqmgi
nllernlennqrvdgklihldihh*hqhyklnlvinmly*ivftmn*krnlkkkriynln
g---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHF830 (SHF830Q) /CSM/SH/SHF8-B/SHF830Q.Seq.d/                       1086   0.0
SHA268 (SHA268Q) /CSM/SH/SHA2-C/SHA268Q.Seq.d/                       1070   0.0
AHD233 (AHD233Q) /CSM/AH/AHD2-B/AHD233Q.Seq.d/                       1070   0.0
AFI676 (AFI676Q) /CSM/AF/AFI6-D/AFI676Q.Seq.d/                       1070   0.0
SHE818 (SHE818Q) /CSM/SH/SHE8-A/SHE818Q.Seq.d/                       1068   0.0
SHD254 (SHD254Q) /CSM/SH/SHD2-C/SHD254Q.Seq.d/                       1068   0.0
SHC618 (SHC618Q) /CSM/SH/SHC6-A/SHC618Q.Seq.d/                       1068   0.0
CHE863 (CHE863Q) /CSM/CH/CHE8-C/CHE863Q.Seq.d/                       1068   0.0
SHL340 (SHL340Q) /CSM/SH/SHL3-B/SHL340Q.Seq.d/                       1066   0.0
SHL163 (SHL163Q) /CSM/SH/SHL1-C/SHL163Q.Seq.d/                       1066   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CF445746|CF445746.1 EST682091 normalized cDNA library of onion Allium cepa cDNA clone ACAHS20, mRNA sequence.    60   1e-08  2
BQ593037|BQ593037.1 E012375-024-028-C05-SP6 MPIZ-ADIS-024-developing root Beta vulgaris cDNA clone 024-028-C05 5-PRIME, mRNA sequence.    44   2e-06  2
CF196667|CF196667.1 Ir64bi1.6_B04 IR64-BI Oryza sativa (indica cultivar-group) cDNA clone IR64-BI1.6B04 5', mRNA sequence.    56   3e-05  2
BG449666|BG449666.1 NF055D11IN1F1092 Insect herbivory Medicago truncatula cDNA clone NF055D11IN 5', mRNA sequence.    58   2e-04  1
BQ141173|BQ141173.1 NF016G07PH1F1056 Phoma-infected Medicago truncatula cDNA clone NF016G07PH 5', mRNA sequence.    58   2e-04  1
DN812276|M2A1_RAT Alpha-mannosidase II (Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase) (MAN II) (Golgi alpha-mannosidase II) (Mannosidase alpha class 2A member 1), mRNA sequence.    56   9e-04  1
CV688215|CV688215.1 sjs2-16-2_G01_sjs2-16-2G01-T3 SJS Schistosoma japonicum cDNA, mRNA sequence.    56   9e-04  1
CV682907|CV682907.1 service43_H06_sjs2-16-2B11-T3 SJS Schistosoma japonicum cDNA, mRNA sequence.    56   9e-04  1
CV682854|CV682854.1 service30_G09_sjs2-14-10C09-T3 SJS Schistosoma japonicum cDNA, mRNA sequence.    56   9e-04  1
BU804021|BU804021.1 SJFBFG06 SJF Schistosoma japonicum cDNA, mRNA sequence.    56   9e-04  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54YC4) RecName: Full=Alpha-mannosidase D;          EC=3.2.1.24...   147   2e-34
(Q54YF7) RecName: Full=Alpha-mannosidase B;          EC=3.2.1.24...   145   5e-34
BC142656_1(BC142656|pid:none) Homo sapiens mannosidase, alpha, c...   140   3e-32
AB011474_23(AB011474|pid:none) Arabidopsis thaliana genomic DNA,...   140   3e-32
(P27046) RecName: Full=Alpha-mannosidase 2;          EC=3.2.1.11...   139   5e-32
AC148217_12(AC148217|pid:none) Medicago truncatula clone mth2-20...   138   1e-31
(P28494) RecName: Full=Alpha-mannosidase 2;          EC=3.2.1.11...   137   1e-31
M24353_1(M24353|pid:none) Rattus norvegicus alpha-mannosidase II...   137   1e-31
AL391146_7(AL391146|pid:none) Arabidopsis thaliana DNA chromosom...   137   2e-31
AK161799_1(AK161799|pid:none) Mus musculus 13 days embryo male t...   136   4e-31

 psg: 0.75  gvh: 0.41  alm: 0.50  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 32.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  8.0 %: mitochondrial

>> prediction for SHF830 is nuc

>SHF830F.Seq
TTTTAATGAAGTTTGCTATTCATTAAAAGATGGTGAAATTAAACTTCATTTAATTCCACA
TTCACATTGTGATTCTGGATGGACATCAACAATGAACGAATATTATATGGGTCAAGTTAA
AAGTATTATATCATCAATGGTTCAATCATTAAATGTTGAATCAAATCCACCTAGAAAATT
TGTTTGGTCTGAAATTGGATTTTTAGAACAATGGTGGGATGATATGCCAATTGAAATTAA
AAATGATTTTATAAAACATGTTAAAAATGATAGGATTGAATTTGTAAATGGTGGATGGGT
AATGAATGATGAAGCATGTGCAAGTTTAGAAAGTGTAATTAGACAATTATCAAATGGGCA
TAAATTTATTAGAGAGAAATTTGGAAAACAACCAGAGAGTGGATGGCAAATTGATCCATT
TGGACATTCATCATTGACACCAACATTACAAGCTCAATTTGGTTATAAACATGTTGTATT
GAATCGTATTCACTATGAATTGAAAAAGAAATTTAAAGAAGAAAAGAATTTACAATTTAA
ATGGAGAGNNNNNNNNNN