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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHF821
Library SH
(Link to library)
Clone ID SHF821
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16417-1|Contig-U16580-1
Original site URL
Representative seq. ID SHF821P
(Link to Original site)
Representative DNA sequence 
>SHF821 (SHF821Q) /CSM/SH/SHF8-A/SHF821Q.Seq.d/
TTACAATTTATCAACATACAATTTTTAAATACTATTCTGCAAATTATTATAAAGTTGAGT
TAATAGTTGGAGGTAAAGTTTATTTTATTCATAAATTTGGTCAATTTAGAGGTGATGATT
GTGAATTTGATGAAATTATTCCAGCTTTTTATAAGAAAGTTCAATTTTTAGATTTATCAA
CACCAATTAAAGCAACAATTCAATTTTCATTTGATTTTTCATCACATCCAAAACATGCAA
ACATTTATTATTCCTGTATAGATGTTTGGTTATTCGACTCTGCATATTACAATTATTGCC
CAACTAATAGTGGTGGTGGAAGTGGTAATAGTGGTGGTAGTTGGTCAAGTGGTGGAAGTG
GTAATAGTGGTGGTGGTTGGTCAAGTGGTGGAAGTGGTAATAGTGGTGGTAGTTGGTCAA
GTGGTAATAGTGGTGGTCATTCTACAGGTGATTGCCCAACAAGTANCGGAGGTTGGACAT
CTGGTANTCACTCAAGTGGATCATGGTCAXXXXXXXXXXAATAGTGGTGCTGCAANCTGG
AGCAAAATAGTGGTGCTGCAANTGAAGCAAATAGTGGCGCTGCAACTGGAGCAAATAGTG
GTGCTGCAACTGGTGCAAATAGTGGAAACAATGCCGCTGNAANTGGTGCTGCAAATGNAA
ATAATGGCGCTGCAGCTGGAGCAAATAGTGGTGCCGCAACTGCTGCAAATAGTGGTNCCG
CAACTGNTGCAAATAGTGGTGCCGCAACTGCTGCAAATAGAGGAAACAATGCCGCTGCAA
CTGGTGCTGCAAATGGAAATAATGGCGCTGCAGCTGGAGCAAATAGTGGTGCCGCAACTG
CTGCAAATAGTGGTGCTGCAAATAGTGGTGCTGCAACTGCTGCAAATAGTGGTGCTGCAG
CTGGAGCAAATAGTGGTGCTGCAACTGCTGCAAATAGTGGCGCTGCAACTGCTGCAAGTG
GTGGAAATGGTGCAAGTGGTGCTGCAAACCNTGGTTCAATAGTAACTCCAAATGATCAAA
ATGTTTCTCCATTATCGAANTCTGATGCTGCAGCAACCGCAGCATCAACTACAGGCAGAA
ACCCAAGAAATCCAACAAGTAGAGCTCCAACGGTCACNCCAACGCCAACNTCTTCAGCTG
AAGAACCAGCTGCTGGTGGCGAAGATTCAAGTGCNATCTCCNAATATAGTATTCNATCAT
TTGGTATCTTTGTTTTATCNATGATAATATATTTAGTAATTTAAAATAACTAATATTCTT
TATATATATAGAAAATAAAATAATAATAATAAAAAAAATT
sequence update 2002.10.25
Translated Amino Acid sequence 
TIYQHTIFKYYSANYYKVELIVGGKVYFIHKFGQFRGDDCEFDEIIPAFYKKVQFLDLST
PIKATIQFSFDFSSHPKHANIYYSCIDVWLFDSAYYNYCPTNSGGGSGNSGGSWSSGGSG
NSGGGWSSGGSGNSGGSWSSGNSGGHSTGDCPTSXGGWTSGXHSSGSWS---

---NSGAAXWSKIVVLQXKQIVALQLEQIVVLQLVQIVETMPLXXVLQMXIMALQLEQIV
VPQLLQIVVPQLXQIVVPQLLQIEETMPLQLVLQMEIMALQLEQIVVPQLLQIVVLQIVV
LQLLQIVVLQLEQIVVLQLLQIVALQLLQVVEMVQVVLQTXVQ**lqmikmflhyrxlml
qqpqhqlqaetqeiqqvelqrsxqrqxlqlknqllvakiqvxspnivfxhlvslfyx**y
i**fkitnilyiyrk*nnnnkkn
Translated Amino Acid sequence (All Frames) 
Frame A:
lqfiniqflntilqiiikls**levkfilfinlvnlevmivnlmklfqlfirkfnf*iyq
hqlkqqfnfhlifhhiqnmqtfiipv*mfgystlhitiiaqlivvvevvivvvvgqvvev
vivvvvgqvvevvivvvvgqvvivvvilqviaqqvxevghlvxtqvdhg---

---NSGAAXWSKIVVLQXKQIVALQLEQIVVLQLVQIVETMPLXXVLQMXIMALQLEQIV
VPQLLQIVVPQLXQIVVPQLLQIEETMPLQLVLQMEIMALQLEQIVVPQLLQIVVLQIVV
LQLLQIVVLQLEQIVVLQLLQIVALQLLQVVEMVQVVLQTXVQ**lqmikmflhyrxlml
qqpqhqlqaetqeiqqvelqrsxqrqxlqlknqllvakiqvxspnivfxhlvslfyx**y
i**fkitnilyiyrk*nnnnkkn

Frame B:
ynlstynf*ilfckll*s*vnswr*slfys*iwsi*r**l*i**nyssfl*essifrfin
tn*snnsifi*ffitsktckhllflyrclvirlcilqllpn**wwwkw**ww*lvkwwkw
**wwwlvkwwkw**ww*lvkw**wwsfyr*lpnkxrrldiwxslkwimv---

---ivvlqxgak*wccx*sk*wrcnwsk*wccnwck*wkqcrxxwcckxk*wrcswsk*w
crncck*wxrnxck*wcrncck*rkqcrcnwcckwk*wrcswsk*wcrncck*wcck*wc
cncck*wccswsk*wccncck*wrcncckwwkwckwcckpwfnsnsk*skcfsiiex*cc
snrsinyrqkpkksnk*ssnghxnanxfs*rtscwwrrfkcxlxi*ysiiwylcfixdni
fsnlk*liffiyienkiiiikki

Frame C:
TIYQHTIFKYYSANYYKVELIVGGKVYFIHKFGQFRGDDCEFDEIIPAFYKKVQFLDLST
PIKATIQFSFDFSSHPKHANIYYSCIDVWLFDSAYYNYCPTNSGGGSGNSGGSWSSGGSG
NSGGGWSSGGSGNSGGSWSSGNSGGHSTGDCPTSXGGWTSGXHSSGSWS---

---*wccxleqnsgaaxeansgaatgansgaatgansgnnaaxxgaanxnngaaagansg
aataansgxatxansgaataanrgnnaaatgaangnngaaagansgaataansgaansga
ataansgaaagansgaataansgaataasggngasgaanxgsivtpndqnvsplsxsdaa
ataasttgrnprnptsraptvtptptssaeepaaggedssaisxysixsfgifvlsmiiy
lvi*nn*yslyi*kik****kk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHF821 (SHF821Q) /CSM/SH/SHF8-A/SHF821Q.Seq.d/                       1396   0.0
SHK112 (SHK112Q) /CSM/SH/SHK1-A/SHK112Q.Seq.d/                       1271   0.0
SHA440 (SHA440Q) /CSM/SH/SHA4-B/SHA440Q.Seq.d/                       1271   0.0
SHC416 (SHC416Q) /CSM/SH/SHC4-A/SHC416Q.Seq.d/                       1267   0.0
AHL869 (AHL869Q) /CSM/AH/AHL8-C/AHL869Q.Seq.d/                       1265   0.0
SHF136 (SHF136Q) /CSM/SH/SHF1-B/SHF136Q.Seq.d/                       1251   0.0
SLH856 (SLH856Q) /CSM/SL/SLH8-C/SLH856Q.Seq.d/                       1249   0.0
SHA456 (SHA456Q) /CSM/SH/SHA4-C/SHA456Q.Seq.d/                       1249   0.0
CHM242 (CHM242Q) /CSM/CH/CHM2-B/CHM242Q.Seq.d/                       1249   0.0
CHR187 (CHR187Q) /CSM/CH/CHR1-D/CHR187Q.Seq.d/                       1247   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CK783714|CK783714.1 UI-D-GC1-aau-c-07-0-UI.s1 UI-D-GC1 Alexandrium tamarense cDNA cloneUI-D-GC1-aau-c-07-0-UI 3', mRNA sequence.    30   0.007  3
DT338982|DT338982.1 JBW092C02.b_012.abi Pineapple week 5-10 nematode-infected gall cDNA library Ananas comosus cDNA clone JBW092C02 similar to undiscovered sequence, mRNA sequence.    30   0.007  3
AC141063|AC141063.1 Homo sapiens chromosome 16 clone RP11-1256C5, WORKING DRAFT SEQUENCE, 11 unordered pieces.    30   0.016  3
AC025169|AC025169.5 Homo sapiens chromosome 5 clone CTC-352M6, complete sequence.    52   0.032  1
AC010461|AC010461.7 Homo sapiens chromosome 5 clone CTD-2275A5, complete sequence.    52   0.032  1
AC141261|AC141261.1 Homo sapiens chromosome 16 clone RP11-1335J10, WORKING DRAFT SEQUENCE, 4 unordered pieces.    28   0.056  3
AC167065|AC167065.1 Xenopus tropicalis clone ISB1-217D23, WORKING DRAFT SEQUENCE, 2 unordered pieces.    28   0.065  3
AL929085|AL929085.8 Zebrafish DNA sequence from clone CH211-11G18 in linkage group 21.    50   0.13   1
AY887028|AY887028.1 Ophiostoma novo-ulmi subsp. novo-ulmi isolate R66 MAT-1-2 gene, complete cds.    34   0.35   2
AY887027|AY887027.1 Ophiostoma novo-ulmi subsp. novo-ulmi isolate YU1 MAT-1-2 gene, complete cds.    34   0.35   2
dna update 2006. 3.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54GV8) RecName: Full=Uncharacterized transmembrane protein DDB...   221   5e-56
AE017244_553(AE017244|pid:none) Mycoplasma hyopneumoniae 7448, c...    35   7.3
AE017243_539(AE017243|pid:none) Mycoplasma hyopneumoniae J, comp...    35   7.3
protein update 2009. 4.18
PSORT 

 psg: 0.61  gvh: 0.32  alm: 0.17  top: 0.47  tms: 0.20  mit: 0.30  mip: 0.07
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: endoplasmic reticulum
	 28.0 %: plasma membrane
	 20.0 %: mitochondrial
	  8.0 %: nuclear
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal
	  4.0 %: Golgi

>> prediction for SHF821 is end
5' end seq. ID SHF821F
5' end seq. 
>SHF821F.Seq
TTACAATTTATCAACATACAATTTTTAAATACTATTCTGCAAATTATTATAAAGTTGAGT
TAATAGTTGGAGGTAAAGTTTATTTTATTCATAAATTTGGTCAATTTAGAGGTGATGATT
GTGAATTTGATGAAATTATTCCAGCTTTTTATAAGAAAGTTCAATTTTTAGATTTATCAA
CACCAATTAAAGCAACAATTCAATTTTCATTTGATTTTTCATCACATCCAAAACATGCAA
ACATTTATTATTCCTGTATAGATGTTTGGTTATTCGACTCTGCATATTACAATTATTGCC
CAACTAATAGTGGTGGTGGAAGTGGTAATAGTGGTGGTAGTTGGTCAAGTGGTGGAAGTG
GTAATAGTGGTGGTGGTTGGTCAAGTGGTGGAAGTGGTAATAGTGGTGGTAGTTGGTCAA
GTGGTAATAGTGGTGGTCATTCTACAGGTGATTGCCCAACAAGTANCGGAGGTTGGACAT
CTGGTANTCACTCAAGTGGATCATGGTCANNNNNNNNNN
Length of 5' end seq. 519
3' end seq. ID SHF821Z
3' end seq. 
>SHF821Z.Seq
NNNNNNNNNNAATAGTGGTGCTGCAANCTGGAGCAAAATAGTGGTGCTGCAANTGAAGCA
AATAGTGGCGCTGCAACTGGAGCAAATAGTGGTGCTGCAACTGGTGCAAATAGTGGAAAC
AATGCCGCTGNAANTGGTGCTGCAAATGNAAATAATGGCGCTGCAGCTGGAGCAAATAGT
GGTGCCGCAACTGCTGCAAATAGTGGTNCCGCAACTGNTGCAAATAGTGGTGCCGCAACT
GCTGCAAATAGAGGAAACAATGCCGCTGCAACTGGTGCTGCAAATGGAAATAATGGCGCT
GCAGCTGGAGCAAATAGTGGTGCCGCAACTGCTGCAAATAGTGGTGCTGCAAATAGTGGT
GCTGCAACTGCTGCAAATAGTGGTGCTGCAGCTGGAGCAAATAGTGGTGCTGCAACTGCT
GCAAATAGTGGCGCTGCAACTGCTGCAAGTGGTGGAAATGGTGCAAGTGGTGCTGCAAAC
CNTGGTTCAATAGTAACTCCAAATGATCAAAATGTTTCTCCATTATCGAANTCTGATGCT
GCAGCAACCGCAGCATCAACTACAGGCAGAAACCCAAGAAATCCAACAAGTAGAGCTCCA
ACGGTCACNCCAACGCCAACNTCTTCAGCTGAAGAACCAGCTGCTGGTGGCGAAGATTCA
AGTGCNATCTCCNAATATAGTATTCNATCATTTGGTATCTTTGTTTTATCNATGATAATA
TATTTAGTAATTTAAAATAACTAATATTCTTTATATATATAGAAAATAAAATAATAATAA
TAAAAAAAATT
Length of 3' end seq. 791
Connected seq. ID SHF821P
Connected seq. 
>SHF821P.Seq
TTACAATTTATCAACATACAATTTTTAAATACTATTCTGCAAATTATTATAAAGTTGAGT
TAATAGTTGGAGGTAAAGTTTATTTTATTCATAAATTTGGTCAATTTAGAGGTGATGATT
GTGAATTTGATGAAATTATTCCAGCTTTTTATAAGAAAGTTCAATTTTTAGATTTATCAA
CACCAATTAAAGCAACAATTCAATTTTCATTTGATTTTTCATCACATCCAAAACATGCAA
ACATTTATTATTCCTGTATAGATGTTTGGTTATTCGACTCTGCATATTACAATTATTGCC
CAACTAATAGTGGTGGTGGAAGTGGTAATAGTGGTGGTAGTTGGTCAAGTGGTGGAAGTG
GTAATAGTGGTGGTGGTTGGTCAAGTGGTGGAAGTGGTAATAGTGGTGGTAGTTGGTCAA
GTGGTAATAGTGGTGGTCATTCTACAGGTGATTGCCCAACAAGTANCGGAGGTTGGACAT
CTGGTANTCACTCAAGTGGATCATGGTCA----------AATAGTGGTGCTGCAANCTGG
AGCAAAATAGTGGTGCTGCAANTGAAGCAAATAGTGGCGCTGCAACTGGAGCAAATAGTG
GTGCTGCAACTGGTGCAAATAGTGGAAACAATGCCGCTGNAANTGGTGCTGCAAATGNAA
ATAATGGCGCTGCAGCTGGAGCAAATAGTGGTGCCGCAACTGCTGCAAATAGTGGTNCCG
CAACTGNTGCAAATAGTGGTGCCGCAACTGCTGCAAATAGAGGAAACAATGCCGCTGCAA
CTGGTGCTGCAAATGGAAATAATGGCGCTGCAGCTGGAGCAAATAGTGGTGCCGCAACTG
CTGCAAATAGTGGTGCTGCAAATAGTGGTGCTGCAACTGCTGCAAATAGTGGTGCTGCAG
CTGGAGCAAATAGTGGTGCTGCAACTGCTGCAAATAGTGGCGCTGCAACTGCTGCAAGTG
GTGGAAATGGTGCAAGTGGTGCTGCAAACCNTGGTTCAATAGTAACTCCAAATGATCAAA
ATGTTTCTCCATTATCGAANTCTGATGCTGCAGCAACCGCAGCATCAACTACAGGCAGAA
ACCCAAGAAATCCAACAAGTAGAGCTCCAACGGTCACNCCAACGCCAACNTCTTCAGCTG
AAGAACCAGCTGCTGGTGGCGAAGATTCAAGTGCNATCTCCNAATATAGTATTCNATCAT
TTGGTATCTTTGTTTTATCNATGATAATATATTTAGTAATTTAAAATAACTAATATTCTT
TATATATATAGAAAATAAAATAATAATAATAAAAAAAATT
Length of connected seq. 1290
Full length Seq ID -
Full length Seq. -
Length of full length seq. -