>AFA171 (AFA171Q) /CSM/AF/AFA1-C/AFA171Q.Seq.d/
ACTGTTGGCCTACTGGGATCCACACACACATAATATATATACACCAAAAATGGAATTGGG
AAAGGAGCAACTTGAAGGAAATTGGTAATTGCCAAGGATTCAAACAAAGCTATCCAAGGA
TTATAATAAAATTCTCACTATTCAAGGAATCACCAGGATGATATTAAAGAGGAAGCAATT
CTTAGATTAGCAAAATTATTTGTAAAGATTGGTAAAGGTGATCAATTACCAACACTTTTA
AGATCAGTTAGACCATTTTTTGATAAAATTTCTAAACCAAAGACTGATAAAATCGTTAGA
AATTTTATTGATATTTTCTCAACCGTACCTGATAATTTAACAACTTTAATTGAATTTGTC
AAAGAGAATATTCAATGGTGTAAAGATACAAATAGAATTTATTTAAGACAAAGATTAGAA
ACTAAACTTTTTACATTAATGTTTGAAGCAAAAGATTATGCAAATGCATTAAGCGGATTA
ACAACATTATTAACAGAAATTAAAAGATTGGATGATAAACCATTATTAGTTGAAATTCAA
CTTGTTXXXXXXXXXXGCTGAAATCGATATGCAATCTGGTATTTTACATTCTGAAGAAAA
AGATTATAAAACTGCTTTTTCATACTTTTTCGAATCATATGAAACTTATGATAGTCTTNA
AGATCCATTTGCAATGAAAGCATTAAAGTATATGTTACTTTGTAAAATTATGACCAACCA
AACTGATGATGTTCATGCATTAGTTAATGGTAAAATTGGTTTGAAATATCATAACAATAG
ATCCATTGAAGCAATTACTCAAATTTCAAAATCTCATGCAAAGAGATCACTTCATATGTT
CCAAGAGGTAACCAAAGAATTTTCTGATCAATTATCTGATGATCCAATCATTCATAGTCA
TTTAACTGAATTATATAGTAATCTTCTTGAACAAAATTTATGTCGTATTATTGAACCATT
CTCAAGGGTTGAAATTTCACATATTGCAAAATTAATTGATTTACCAGTTGATGTTGTTGA
AAGAAAATTATCACTTATGACCCTTGATAAGAAATATAATGGTATCCTCGATCAAGGTAC
TGGTACTTTAATTGTATTTGAAGAACAAAAAGAAGATAAATTATTTAATTGTTCCTTTAG
AAACTATTGGTGCTTTAAGTCGTGTTGTTGATTTACTTTANGAAAANA

tvgllgstht*yiytkngigkgat*rklviakdsnkaiqgl**NSHYSRNHQDDIKEEAI
LRLAKLFVKIGKGDQLPTLLRSVRPFFDKISKPKTDKIVRNFIDIFSTVPDNLTTLIEFV
KENIQWCKDTNRIYLRQRLETKLFTLMFEAKDYANALSGLTTLLTEIKRLDDKPLLVEIQ
LV---

---AEIDMQSGILHSEEKDYKTAFSYFFESYETYDSLXDPFAMKALKYMLLCKIMTNQTD
DVHALVNGKIGLKYHNNRSIEAITQISKSHAKRSLHMFQEVTKEFSDQLSDDPIIHSHLT
ELYSNLLEQNLCRIIEPFSRVEISHIAKLIDLPVDVVERKLSLMTLDKKYNGILDQGTGT
LIVFEEQKEDKLFNCSFRNYWCFKSCC*ftlxk

Frame A:
tvgllgstht*yiytkngigkgat*rklviakdsnkaiqgl**NSHYSRNHQDDIKEEAI
LRLAKLFVKIGKGDQLPTLLRSVRPFFDKISKPKTDKIVRNFIDIFSTVPDNLTTLIEFV
KENIQWCKDTNRIYLRQRLETKLFTLMFEAKDYANALSGLTTLLTEIKRLDDKPLLVEIQ
LV---

---AEIDMQSGILHSEEKDYKTAFSYFFESYETYDSLXDPFAMKALKYMLLCKIMTNQTD
DVHALVNGKIGLKYHNNRSIEAITQISKSHAKRSLHMFQEVTKEFSDQLSDDPIIHSHLT
ELYSNLLEQNLCRIIEPFSRVEISHIAKLIDLPVDVVERKLSLMTLDKKYNGILDQGTGT
LIVFEEQKEDKLFNCSFRNYWCFKSCC*ftlxk

Frame B:
llaywdphthniytpkmelgkeqlegnw*lpriqtklskdynkiltiqgitrmilkrkqf
ld*qnyl*rlvkvinyqhf*dqldhflikflnqrliksleillifsqpylii*ql*lnls
krifngvkiqiefi*dkd*klnflh*clkqkimqmh*ad*qhy*qklkdwminhy*lkfn
l---

---lksicnlvfyilkkkiikllfhtfsnhmklmivxkihlq*kh*sicyfvkl*ptklm
mfmh*lmvklv*niitidplkqllkfqnlmqrdhficskr*pknflinylmiqsfivi*l
nyiviflnkiyvvllnhsqglkfhilqn*liyqlmllkenyhl*plirnimvssikvlvl
*lylknkkkinylivpletigalsrvvdllxex

Frame C:
cwptgihthiiyihqkwnwersnlkeigncqgfkqsypriiikfslfkespg*y*rgsns
*iskiickdw*r*sitntfkis*tif**nf*tkd**nr*kfy*yflnrt**fnnfn*icq
reysmv*ryk*nlfktkirn*tfyinv*skrlckcikrinniinrn*kig**tiis*nst
c---

---*nryaiwyftf*rkrl*ncffilfrii*nl**sxrsicnesikvyvtl*nydqpn**
cscis*w*nwfeis*q*ih*snysnfkisckeitsyvprgnqrif*sii**snhs*sfn*
ii**ss*tkfmsyy*tilkg*nftyckin*fts*cc*kkiitydp**ei*wyprsrywyf
nci*rtkrr*ii*lfl*kllvl*vvlliyfxkx

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFA171 (AFA171Q) /CSM/AF/AFA1-C/AFA171Q.Seq.d/                       2280   0.0
VFH786 (VFH786Q) /CSM/VF/VFH7-D/VFH786Q.Seq.d/                       1207   0.0
VFF145 (VFF145Q) /CSM/VF/VFF1-B/VFF145Q.Seq.d/                       1207   0.0
SFI676 (SFI676Q) /CSM/SF/SFI6-D/SFI676Q.Seq.d/                       1207   0.0
SFI670 (SFI670Q) /CSM/SF/SFI6-C/SFI670Q.Seq.d/                       1207   0.0
SFB566 (SFB566Q) /CSM/SF/SFB5-C/SFB566Q.Seq.d/                       1207   0.0
CFF680 (CFF680Q) /CSM/CF/CFF6-D/CFF680Q.Seq.d/                       1207   0.0
AFO642 (AFO642Q) /CSM/AF/AFO6-B/AFO642Q.Seq.d/                       1207   0.0
AFN307 (AFN307Q) /CSM/AF/AFN3-A/AFN307Q.Seq.d/                       1207   0.0
AFM375 (AFM375Q) /CSM/AF/AFM3-D/AFM375Q.Seq.d/                       1207   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AX488960|AX488960.1 Sequence 6260 from Patent WO02053728.         74  1e-19  4
AL408021|AL408021.1 T7 end of clone AV0AA006B01 of library AV0AA from strain CBS 379 of Saccharomyces exiguus.    52  1e-06  3
BI699197|BI699197.1 sag34b03.y1 Gm-c1081 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1081-462 5' similar to TR:O81694 O81694 19S PROTEOSOME SUBUNIT 9. ;, mRNA sequence.    46  2e-06  2
AW706890|AW706890.1 sk07g03.y1 Gm-c1023 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1023-3437 5' similar to TR:O81694 O81694 19S PROTEOSOME SUBUNIT 9. ;, mRNA sequence.    46  2e-06  2
CD413771|CD413771.1 Gm_ck45395 Soybean induced by Salicylic Acid Glycine max cDNA 3', mRNA sequence.    46  3e-06  2
AL929353|AL929353.1 Plasmodium falciparum strain 3D7, chromosome 5, segment 3/4.    36  0.005  14
AC018622|AC018622.10 Homo sapiens chromosome 8, clone RP11-346M13, complete sequence.    34  0.027  10
AP004173|AP004173.1 Mycoplasma penetrans genomic DNA, complete sequence, section 4/5.    34  0.035  13
CD719657|CD719657.1 VVB163F05_411221 An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVB163F05 5, mRNA sequence.    50  0.063  1
CD718637|CD718637.1 VVB152D08_409181 An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVB152D08 5, mRNA sequence.    50  0.063  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54UB5) RecName: Full=26S proteasome non-ATPase regulatory subu...   381   e-178
AM424416_1(AM424416|pid:none) Vitis vinifera contig VV78X196128....   234   5e-85
AY230834_1(AY230834|pid:none) Arabidopsis thaliana 26S proteasom...   231   9e-85
A86414(A86414) hypothetical protein F28N24.15 - Arabidopsis thal...   231   2e-84
AY230837_1(AY230837|pid:none) Arabidopsis thaliana 26S proteasom...   231   2e-84
BT053774_1(BT053774|pid:none) Zea mays full-length cDNA clone ZM...   231   2e-83
EU969379_1(EU969379|pid:none) Zea mays clone 329471 26S proteaso...   230   3e-83
AK064788_1(AK064788|pid:none) Oryza sativa Japonica Group cDNA c...   230   5e-83
BT035753_1(BT035753|pid:none) Zea mays full-length cDNA clone ZM...   229   4e-82
AF083890_1(AF083890|pid:none) Arabidopsis thaliana 19S proteosom...   218   2e-80

 psg: 0.75  gvh: 0.39  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 32.0 %: cytoplasmic
	 20.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for AFA171 is nuc

>AFA171F.Seq
ACTGTTGGCCTACTGGGATCCACACACACATAATATATATACACCAAAAATGGAATTGGG
AAAGGAGCAACTTGAAGGAAATTGGTAATTGCCAAGGATTCAAACAAAGCTATCCAAGGA
TTATAATAAAATTCTCACTATTCAAGGAATCACCAGGATGATATTAAAGAGGAAGCAATT
CTTAGATTAGCAAAATTATTTGTAAAGATTGGTAAAGGTGATCAATTACCAACACTTTTA
AGATCAGTTAGACCATTTTTTGATAAAATTTCTAAACCAAAGACTGATAAAATCGTTAGA
AATTTTATTGATATTTTCTCAACCGTACCTGATAATTTAACAACTTTAATTGAATTTGTC
AAAGAGAATATTCAATGGTGTAAAGATACAAATAGAATTTATTTAAGACAAAGATTAGAA
ACTAAACTTTTTACATTAATGTTTGAAGCAAAAGATTATGCAAATGCATTAAGCGGATTA
ACAACATTATTAACAGAAATTAAAAGATTGGATGATAAACCATTATTAGTTGAAATTCAA
CTTGTT----------

>AFA171Z.Seq
----------GCTGAAATCGATATGCAATCTGGTATTTTACATTCTGAAGAAAAAGATTA
TAAAACTGCTTTTTCATACTTTTTCGAATCATATGAAACTTATGATAGTCTTNAAGATCC
ATTTGCAATGAAAGCATTAAAGTATATGTTACTTTGTAAAATTATGACCAACCAAACTGA
TGATGTTCATGCATTAGTTAATGGTAAAATTGGTTTGAAATATCATAACAATAGATCCAT
TGAAGCAATTACTCAAATTTCAAAATCTCATGCAAAGAGATCACTTCATATGTTCCAAGA
GGTAACCAAAGAATTTTCTGATCAATTATCTGATGATCCAATCATTCATAGTCATTTAAC
TGAATTATATAGTAATCTTCTTGAACAAAATTTATGTCGTATTATTGAACCATTCTCAAG
GGTTGAAATTTCACATATTGCAAAATTAATTGATTTACCAGTTGATGTTGTTGAAAGAAA
ATTATCACTTATGACCCTTGATAAGAAATATAATGGTATCCTCGATCAAGGTACTGGTAC
TTTAATTGTATTTGAAGAACAAAAAGAAGATAAATTATTTAATTGTTCCTTTAGAAACTA
TTGGTGCTTTAAGTCGTGTTGTTGATTTACTTTANGAAAANA

>AFA171P.Seq
ACTGTTGGCCTACTGGGATCCACACACACATAATATATATACACCAAAAATGGAATTGGG
AAAGGAGCAACTTGAAGGAAATTGGTAATTGCCAAGGATTCAAACAAAGCTATCCAAGGA
TTATAATAAAATTCTCACTATTCAAGGAATCACCAGGATGATATTAAAGAGGAAGCAATT
CTTAGATTAGCAAAATTATTTGTAAAGATTGGTAAAGGTGATCAATTACCAACACTTTTA
AGATCAGTTAGACCATTTTTTGATAAAATTTCTAAACCAAAGACTGATAAAATCGTTAGA
AATTTTATTGATATTTTCTCAACCGTACCTGATAATTTAACAACTTTAATTGAATTTGTC
AAAGAGAATATTCAATGGTGTAAAGATACAAATAGAATTTATTTAAGACAAAGATTAGAA
ACTAAACTTTTTACATTAATGTTTGAAGCAAAAGATTATGCAAATGCATTAAGCGGATTA
ACAACATTATTAACAGAAATTAAAAGATTGGATGATAAACCATTATTAGTTGAAATTCAA
CTTGTT----------GCTGAAATCGATATGCAATCTGGTATTTTACATTCTGAAGAAAA
AGATTATAAAACTGCTTTTTCATACTTTTTCGAATCATATGAAACTTATGATAGTCTTNA
AGATCCATTTGCAATGAAAGCATTAAAGTATATGTTACTTTGTAAAATTATGACCAACCA
AACTGATGATGTTCATGCATTAGTTAATGGTAAAATTGGTTTGAAATATCATAACAATAG
ATCCATTGAAGCAATTACTCAAATTTCAAAATCTCATGCAAAGAGATCACTTCATATGTT
CCAAGAGGTAACCAAAGAATTTTCTGATCAATTATCTGATGATCCAATCATTCATAGTCA
TTTAACTGAATTATATAGTAATCTTCTTGAACAAAATTTATGTCGTATTATTGAACCATT
CTCAAGGGTTGAAATTTCACATATTGCAAAATTAATTGATTTACCAGTTGATGTTGTTGA
AAGAAAATTATCACTTATGACCCTTGATAAGAAATATAATGGTATCCTCGATCAAGGTAC
TGGTACTTTAATTGTATTTGAAGAACAAAAAGAAGATAAATTATTTAATTGTTCCTTTAG
AAACTATTGGTGCTTTAAGTCGTGTTGTTGATTTACTTTANGAAAANA