>SHF645 (SHF645Q) /CSM/SH/SHF6-B/SHF645Q.Seq.d/
ATCATTGTTAATAGCAAAAGAAAAATGGCAACAAAAAATACATCTCCTTATGAGATTGAA
TATGCAAAGAGTGATAGATCAACATGTTCAACCTGTCAAAGAGGTATTAATAAAGAAGCA
GTTCGTATTGGTTATAAAACAAAATCAAAACACTTTGATGGAATGGATGTATCATGGCAT
CATTTAAAATGTAAATGTCCACAAGTACCATCATTTACAGATTTAATTCACTGGGAATAC
CTTCGTTGGGAAGACCAATTATCAATTAAAACAACTTATTTTTCATCTGAAAAGTATGAT
CCAAAATCAGCATCAGAGGTACAAAGAGAGAAATATTTAAAGGCATTATGGGAAGTTAAA
GATAGTATTGCCGATAACTTGAAGGGACCAGCAATTAAAAACATCATTCAATATAATAAA
GGTTATGTTGATAAGGTATCACCAGTTCATTTACTTCATACTCTCTCTGATTGGATGTTA
AAAGGTCGTCCAGGTAGATGTCCAACTTGTAAGAATTTCGATATACTTTTCAATGGTATC
GAATATCAATGTAAAGGTTGGATTTCAGGTTTCACAAAATGTGATTGGAAAGGTGATTCA
ATTGAAAGATGGGCTGTTCAATTTCCTGATGATCTTTCAXXXXXXXXXXAAGAAGCAAAC
TGAATCACTCGAGGGTAACATGATTGAAAATCAATATATTACACTCAAAACCAAACTCAC
TNCCACTTGATAAAGATAGTGTAGTTTATAAAACCTTGGTAGATTATGTTGCCAATAGTC
AAGATAAACAATTTAGAACCAATATCTGTGTTCAAGATATTCTTTCACTTGAGAGAGAGA
ATGAAAGTGAACGTTTCACTCNTTGGGCAAAAGATAAGAATAGATTACTCTTATGGCATG
GTAGTAGGTTAACTAATTTCGTATCGATTGTCTCGCAGGGATTAAGAATTGCTCCACCAT
CTGCTCCAAAAACTGGTTACAGATTTGGTAAAGGTATTTATTTTGCTGATTGTATTAGTA
AATCATTCAGCTATTGTTTCACATCAAGTGATTGTCCAACTGCACTTATGTTATTATGTG
AAGTTTCACTTGGTGACATGAATGAATTGAAACATGATACNTATATGGAAGAGGCACCAC
ATCCATTCCATAGTACAAAAGCTTTAGGTATGGCTGCTCCTCACAAAGATGGTAATCATC
CTCTTTCTGATTCTGATGGTTTAGTAGTACCATTAGGTAAAATTTCAAAGACTGGTCTCT
CAACTTCATGTACTCACAATGAATTCATTGTCTATAAAATTGAACAAGTCAGAATCAAAT
ACATCTTAAGAGTTTACAANGAAAATAAAATAAAATAAAATTTTTTAAAATTATCCTTAA
ATTTT

IIVNSKRKMATKNTSPYEIEYAKSDRSTCSTCQRGINKEAVRIGYKTKSKHFDGMDVSWH
HLKCKCPQVPSFTDLIHWEYLRWEDQLSIKTTYFSSEKYDPKSASEVQREKYLKALWEVK
DSIADNLKGPAIKNIIQYNKGYVDKVSPVHLLHTLSDWMLKGRPGRCPTCKNFDILFNGI
EYQCKGWISGFTKCDWKGDSIERWAVQFPDDLS---

---KKQTESLEGNMIENQYITLKTKLTXT**r*csl*nlgrlccq*sr*ti*nqylcsry
sft*ere*k*tfhslgkr*e*itlmaw**vn*fridclagikncsticsknwlqiw*ryl
fc*ly**iiqllfhik*lsnctyvim*sftw*he*iet*yxygrgttsip*yksfrygcs
sqrw*sssf*f*wfsstir*nfkdwslnfmysq*ihcl*n*tsqnqihlkslqxk*nkik
ffkiilkf

Frame A:
IIVNSKRKMATKNTSPYEIEYAKSDRSTCSTCQRGINKEAVRIGYKTKSKHFDGMDVSWH
HLKCKCPQVPSFTDLIHWEYLRWEDQLSIKTTYFSSEKYDPKSASEVQREKYLKALWEVK
DSIADNLKGPAIKNIIQYNKGYVDKVSPVHLLHTLSDWMLKGRPGRCPTCKNFDILFNGI
EYQCKGWISGFTKCDWKGDSIERWAVQFPDDLS---

---KKQTESLEGNMIENQYITLKTKLTXT**r*csl*nlgrlccq*sr*ti*nqylcsry
sft*ere*k*tfhslgkr*e*itlmaw**vn*fridclagikncsticsknwlqiw*ryl
fc*ly**iiqllfhik*lsnctyvim*sftw*he*iet*yxygrgttsip*yksfrygcs
sqrw*sssf*f*wfsstir*nfkdwslnfmysq*ihcl*n*tsqnqihlkslqxk*nkik
ffkiilkf

Frame B:
slliakekwqqkihllmrlnmqrvidqhvqpvkevlikkqfvlvikqnqntlmewmyhgi
i*nvnvhkyhhlqi*ftgntfvgktnyqlkqlifhlksmiqnqhqrykerni*rhygklk
ivlpit*rdqqlktsfniikvmliryhqfiyfilsligc*kvvqvdvqlvrisiyfsmvs
ninvkvgfqvsqnvigkviqlkdglfnflmif---

---rsklnhsrvt*lkinilhskpnslpldkdsvvyktlvdyvansqdkqfrtnicvqdi
lslereneserftxwakdknrlllwhgsrltnfvsivsqglriappsapktgyrfgkgiy
fadcisksfsycftssdcptalmllcevslgdmnelkhdtymeeaphpfhstkalgmaap
hkdgnhplsdsdglvvplgkisktglstscthnefivykieqvrikyilrvyxenkik*n
flklslnf

Frame C:
hc**qkkngnkkyisl*d*icke**inmfnlskry**rsssywl*nkiktl*wngcimas
fkm*mststiiyrfnslgipslgrpiin*nnlffi*kv*skisirgtkreifkgimgs*r
*ycr*legtsn*khhsi**rlc**gitssftsysl*ldvkrssr*msnl*efrytfqwyr
ism*rldfrfhkm*ler*fn*kmgcsis**sf---

---ean*itrg*hd*ksiyytqnqthxhlikiv*fikpw*imlpivkinnlepisvfkif
fhlrermkvnvslxgqkiridysygmvvg*lisyrlsrrd*ellhhllqklvtdlvkvfi
llivlvnhsaivshqvivqlhlcyyvkfhlvt*mn*nmixiwkrhhihsivqkl*vwlll
tkmviilflilmv**yh*vkfqrlvsqlhvltmnslsiklnksesnts*eftxkik*nki
f*nyp*i

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHF645 (SHF645Q) /CSM/SH/SHF6-B/SHF645Q.Seq.d/                       2577   0.0
VHB303 (VHB303Q) /CSM/VH/VHB3-A/VHB303Q.Seq.d/                       1350   0.0
AHB712 (AHB712Q) /CSM/AH/AHB7-A/AHB712Q.Seq.d/                       1350   0.0
AHR444 (AHR444Q) /CSM/AH/AHR4-B/AHR444Q.Seq.d/                       1344   0.0
VHB869 (VHB869Q) /CSM/VH/VHB8-C/VHB869Q.Seq.d/                       1340   0.0
SHD815 (SHD815Q) /CSM/SH/SHD8-A/SHD815Q.Seq.d/                       1336   0.0
CHQ835 (CHQ835Q) /CSM/CH/CHQ8-B/CHQ835Q.Seq.d/                       1336   0.0
AHN663 (AHN663Q) /CSM/AH/AHN6-C/AHN663Q.Seq.d/                       1330   0.0
AHA817 (AHA817Q) /CSM/AH/AHA8-A/AHA817Q.Seq.d/                       1328   0.0
SHJ664 (SHJ664Q) /CSM/SH/SHJ6-C/SHJ664Q.Seq.d/                       1326   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AJ534948|AJ534948.1 Dictyostelium discoideum mRNA for NAD(+) ADP-ribosyltransferase-1A (adprt-1A gene).  1263   0.0    1
AJ520099|AJ520099.1 Dictyostelium discoideum mRNA for (ADP-ribosyl)transferase (adprt2  gene).    62   3e-14  3
DY229813|DY229813.1 ID0AAK8YC07CM1 ApHL3SD Acyrthosiphon pisum cDNA clone ID0AAK8YC07 5', mRNA sequence.    60   1e-04  1
AC025807|AC025807.2 Homo sapiens clone RP11-657G2, WORKING DRAFT SEQUENCE, 15 unordered pieces.    50   0.14   1
AM113503|AM113503.1 Streptococcus sp. 2410 lytA gene for N-acetylmuramoyl-L-alanine  amidase, strain 2410.    50   0.14   1
AM113502|AM113502.1 Streptococcus sp. 3137 lytA gene for N-acetylmuramoyl-L-alanine  amidase, strain 3137.    50   0.14   1
AM113501|AM113501.1 Streptococcus sp. 1956 lytA gene for N-acetylmuramoyl-L-alanine  amidase, strain 1956.    50   0.14   1
AM113500|AM113500.1 Streptococcus sp. 1504 lytA gene for N-acetylmuramoyl-L-alanine  amidase, strain 1504.    50   0.14   1
AM113497|AM113497.1 Streptococcus sp. 578 lytA gene for N-acetylmuramoyl-L-alanine  amidase, strain 578.    50   0.14   1
AM113496|AM113496.1 Streptococcus pseudopneumoniae lytA gene for  N-acetylmuramoyl-L-alanine amidase, strain CCUG 48465.    50   0.14   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

X65497_1(X65497|pid:none) R.norvegicus mRNA for poly(ADP-ribose)...   182   2e-44
(P27008) RecName: Full=Poly [ADP-ribose] polymerase 1;          ...   182   2e-44
PDBF(1A26) MOL_ID: 1; MOLECULE: POLY (ADP-RIBOSE) POLYMERASE; CH...   182   2e-44
(P26446) RecName: Full=Poly [ADP-ribose] polymerase 1;          ...   182   2e-44
AK303340_1(AK303340|pid:none) Homo sapiens cDNA FLJ53442 complet...   181   5e-44
(Q9R152) RecName: Full=Poly [ADP-ribose] polymerase 1;          ...   181   7e-44
AK312339_1(AK312339|pid:none) Homo sapiens cDNA, FLJ92658, highl...   180   9e-44
J03473_1(J03473|pid:none) Human poly(ADP-ribose) synthetase mRNA...   180   9e-44
AB384394_1(AB384394|pid:none) Synthetic construct DNA, clone: pF...   180   9e-44
M17081_1(M17081|pid:none) Human placental poly(ADP-ribose) polym...   180   9e-44

 psg: 0.69  gvh: 0.23  alm: 0.54  top: 0.53  tms: 0.00  mit: 0.38  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 28.0 %: cytoplasmic
	 20.0 %: mitochondrial
	 12.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall

>> prediction for SHF645 is nuc

>SHF645F.Seq
ATCATTGTTAATAGCAAAAGAAAAATGGCAACAAAAAATACATCTCCTTATGAGATTGAA
TATGCAAAGAGTGATAGATCAACATGTTCAACCTGTCAAAGAGGTATTAATAAAGAAGCA
GTTCGTATTGGTTATAAAACAAAATCAAAACACTTTGATGGAATGGATGTATCATGGCAT
CATTTAAAATGTAAATGTCCACAAGTACCATCATTTACAGATTTAATTCACTGGGAATAC
CTTCGTTGGGAAGACCAATTATCAATTAAAACAACTTATTTTTCATCTGAAAAGTATGAT
CCAAAATCAGCATCAGAGGTACAAAGAGAGAAATATTTAAAGGCATTATGGGAAGTTAAA
GATAGTATTGCCGATAACTTGAAGGGACCAGCAATTAAAAACATCATTCAATATAATAAA
GGTTATGTTGATAAGGTATCACCAGTTCATTTACTTCATACTCTCTCTGATTGGATGTTA
AAAGGTCGTCCAGGTAGATGTCCAACTTGTAAGAATTTCGATATACTTTTCAATGGTATC
GAATATCAATGTAAAGGTTGGATTTCAGGTTTCACAAAATGTGATTGGAAAGGTGATTCA
ATTGAAAGATGGGCTGTTCAATTTCCTGATGATCTTTCANNNNNNNNNN

>SHF645Z.Seq
NNNNNNNNNNAAGAAGCAAACTGAATCACTCGAGGGTAACATGATTGAAAATCAATATAT
TACACTCAAAACCAAACTCACTNCCACTTGATAAAGATAGTGTAGTTTATAAAACCTTGG
TAGATTATGTTGCCAATAGTCAAGATAAACAATTTAGAACCAATATCTGTGTTCAAGATA
TTCTTTCACTTGAGAGAGAGAATGAAAGTGAACGTTTCACTCNTTGGGCAAAAGATAAGA
ATAGATTACTCTTATGGCATGGTAGTAGGTTAACTAATTTCGTATCGATTGTCTCGCAGG
GATTAAGAATTGCTCCACCATCTGCTCCAAAAACTGGTTACAGATTTGGTAAAGGTATTT
ATTTTGCTGATTGTATTAGTAAATCATTCAGCTATTGTTTCACATCAAGTGATTGTCCAA
CTGCACTTATGTTATTATGTGAAGTTTCACTTGGTGACATGAATGAATTGAAACATGATA
CNTATATGGAAGAGGCACCACATCCATTCCATAGTACAAAAGCTTTAGGTATGGCTGCTC
CTCACAAAGATGGTAATCATCCTCTTTCTGATTCTGATGGTTTAGTAGTACCATTAGGTA
AAATTTCAAAGACTGGTCTCTCAACTTCATGTACTCACAATGAATTCATTGTCTATAAAA
TTGAACAAGTCAGAATCAAATACATCTTAAGAGTTTACAANGAAAATAAAATAAAATAAA
ATTTTTTAAAATTATCCTTAAATTTT

>SHF645P.Seq
ATCATTGTTAATAGCAAAAGAAAAATGGCAACAAAAAATACATCTCCTTATGAGATTGAA
TATGCAAAGAGTGATAGATCAACATGTTCAACCTGTCAAAGAGGTATTAATAAAGAAGCA
GTTCGTATTGGTTATAAAACAAAATCAAAACACTTTGATGGAATGGATGTATCATGGCAT
CATTTAAAATGTAAATGTCCACAAGTACCATCATTTACAGATTTAATTCACTGGGAATAC
CTTCGTTGGGAAGACCAATTATCAATTAAAACAACTTATTTTTCATCTGAAAAGTATGAT
CCAAAATCAGCATCAGAGGTACAAAGAGAGAAATATTTAAAGGCATTATGGGAAGTTAAA
GATAGTATTGCCGATAACTTGAAGGGACCAGCAATTAAAAACATCATTCAATATAATAAA
GGTTATGTTGATAAGGTATCACCAGTTCATTTACTTCATACTCTCTCTGATTGGATGTTA
AAAGGTCGTCCAGGTAGATGTCCAACTTGTAAGAATTTCGATATACTTTTCAATGGTATC
GAATATCAATGTAAAGGTTGGATTTCAGGTTTCACAAAATGTGATTGGAAAGGTGATTCA
ATTGAAAGATGGGCTGTTCAATTTCCTGATGATCTTTCA----------AAGAAGCAAAC
TGAATCACTCGAGGGTAACATGATTGAAAATCAATATATTACACTCAAAACCAAACTCAC
TNCCACTTGATAAAGATAGTGTAGTTTATAAAACCTTGGTAGATTATGTTGCCAATAGTC
AAGATAAACAATTTAGAACCAATATCTGTGTTCAAGATATTCTTTCACTTGAGAGAGAGA
ATGAAAGTGAACGTTTCACTCNTTGGGCAAAAGATAAGAATAGATTACTCTTATGGCATG
GTAGTAGGTTAACTAATTTCGTATCGATTGTCTCGCAGGGATTAAGAATTGCTCCACCAT
CTGCTCCAAAAACTGGTTACAGATTTGGTAAAGGTATTTATTTTGCTGATTGTATTAGTA
AATCATTCAGCTATTGTTTCACATCAAGTGATTGTCCAACTGCACTTATGTTATTATGTG
AAGTTTCACTTGGTGACATGAATGAATTGAAACATGATACNTATATGGAAGAGGCACCAC
ATCCATTCCATAGTACAAAAGCTTTAGGTATGGCTGCTCCTCACAAAGATGGTAATCATC
CTCTTTCTGATTCTGATGGTTTAGTAGTACCATTAGGTAAAATTTCAAAGACTGGTCTCT
CAACTTCATGTACTCACAATGAATTCATTGTCTATAAAATTGAACAAGTCAGAATCAAAT
ACATCTTAAGAGTTTACAANGAAAATAAAATAAAATAAAATTTTTTAAAATTATCCTTAA
ATTTT