>AFJ364 (AFJ364Q) /CSM/AF/AFJ3-C/AFJ364Q.Seq.d/
XXXXXXXXXXGATGTAAAGAAAATCGCTACCTCACAAAAAGGTAATGCCGTTTTCTGTTG
GGTCGATATGAAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTCTGGTAAAGTTGT
TCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTTCGATGAAAAAGA
AACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGGTGGTAAAGTTTC
TCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCCGT
TGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGGTTGAATTCTA
CGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAATA
CTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTACC
AAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAAGA
AAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTGAATTCATTCA
AGATAACGCTGCCATGAATTCAAATCACCATCATCTCAAACNANATAATGTNAATCTAAA
AAAGATTCTTC

---VKKIATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKET
FSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFYA
PWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKEN
PISYEGQRNDHMNFVEFIQDNAAMNSNHHHLKXXNVNLKKIL

Frame A:
---m*rkslphkkvmpfsvgsi*rnshnkqliwvslvklfqpslliqlpiklvtismkkk
pshsipspngskmslvvkflhllnlnqnqnqmmlqlrlplvplskswf*thqrmfwlnst
lhgaviarillqstin*vnt*ktlnlsqs*rlmptqmmyqvisklevtqpscssklmtkk
iqslmkvkemii*tllnsfkitlp*iqitiisnximxi*krff


Frame B:
---ckenryltkr*crfllgryeeipttsnsygslw*scsshlc*fscq*sslqfr*krn
lliryrlqmdprchww*sfsic*istrtrik*css*gcrwyhfqkvgfrltkgcfg*ilr
smvrslqescsnlr*ir*ilkrr*iclnrkd*crlk*ctk*yrn*rlpnhhalqs**qrk
snll*rskk*syelc*ihsr*rchefkspssqtx*cxskkds


Frame C:
---VKKIATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKET
FSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFYA
PWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKEN
PISYEGQRNDHMNFVEFIQDNAAMNSNHHHLKXXNVNLKKIL

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFJ364 (AFJ364Q) /CSM/AF/AFJ3-C/AFJ364Q.Seq.d/                       1292   0.0
AFK889 (AFK889Q) /CSM/AF/AFK8-D/AFK889Q.Seq.d/                       1285   0.0
AFK858 (AFK858Q) /CSM/AF/AFK8-C/AFK858Q.Seq.d/                       1271   0.0
AFH860 (AFH860Q) /CSM/AF/AFH8-C/AFH860Q.Seq.d/                       1271   0.0
AFH769 (AFH769Q) /CSM/AF/AFH7-C/AFH769Q.Seq.d/                       1271   0.0
AFH744 (AFH744Q) /CSM/AF/AFH7-B/AFH744Q.Seq.d/                       1271   0.0
AFM618 (AFM618Q) /CSM/AF/AFM6-A/AFM618Q.Seq.d/                       1265   0.0
AFL139 (AFL139Q) /CSM/AF/AFL1-B/AFL139Q.Seq.d/                       1265   0.0
AFJ434 (AFJ434Q) /CSM/AF/AFJ4-B/AFJ434Q.Seq.d/                       1265   0.0
AFN313 (AFN313Q) /CSM/AF/AFN3-A/AFN313Q.Seq.d/                       1263   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end,single read.   599   e-167  1
CN206669|CN206669.1 Tor7097 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    78   2e-10  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN590755|CN590755.1 TTE00006978 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN591465|CN591465.1 TTE00010235 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN597971|CN597971.1 TTE00009997 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CB176061|CB176061.1 pl17e07.y3 Ancylostoma ceylanicum adult Ancylostoma ceylanicum cDNA 5' similar to TR:O17908 O17908 H06O01.1 PROTEIN. [1] ;, mRNA sequence.    70   5e-08  1
CK030146|CK030146.1 AGENCOURT_16621384 NIH_ZGC_7 Danio rerio cDNA clone IMAGE:7052631 5', mRNA sequence.    70   5e-08  1
AL924030|AL924030.1 Danio rerio EST sequence, clone 134-B07-2.     70   5e-08  1
BC067155|BC067155.1 Danio rerio zgc:77086, mRNA (cDNA clone MGC:77086 IMAGE:6960343), complete cds.    70   5e-08  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   171   1e-41
EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   170   3e-41
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   167   3e-40
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   165   9e-40
CU633901_146(CU633901|pid:none) Podospora anserina genomic DNA c...   160   2e-38
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   160   3e-38
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   151   2e-35
AK290971_1(AK290971|pid:none) Homo sapiens cDNA FLJ78033 complet...   150   2e-35
BC046707_1(BC046707|pid:none) Xenopus laevis glucose regulated p...   150   2e-35
(P13667) RecName: Full=Protein disulfide-isomerase A4;          ...   150   2e-35

 psg: 0.86  gvh: 0.36  alm: 0.43  top: 0.47  tms: 0.00  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for AFJ364 is cyt

>AFJ364Z.Seq
----------GATGTAAAGAAAATCGCTACCTCACAAAAAGGTAATGCCGTTTTCTGTTG
GGTCGATATGAAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTCTGGTAAAGTTGT
TCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTTCGATGAAAAAGA
AACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGGTGGTAAAGTTTC
TCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCCGT
TGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGGTTGAATTCTA
CGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAATA
CTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTACC
AAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAAGA
AAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTGAATTCATTCA
AGATAACGCTGCCATGAATTCAAATCACCATCATCTCAAACNANATAATGTNAATCTAAA
AAAGATTCTTC