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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFJ353
Library AF
(Link to library)
Clone ID AFJ353
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10972-1
Original site URL
Representative seq. ID AFJ353P
(Link to Original site)
Representative DNA sequence 
>AFJ353 (AFJ353Q) /CSM/AF/AFJ3-C/AFJ353Q.Seq.d/
AAGCCCCATTCAAATCTCCAAGAAAATTAAATACATCTGGTAGTGGATTAACTTCAATAA
TCCCACCAGGTTTTTCAAACGAATCTTCACCAAATACATCAAGCAATATTGTATCTCCAT
CAACTACAACCACCACACCATTATCATCAACTTCACCATCAACCACAACCACAACCACTA
CACCAACATCATTCCAACCAAGTGGAGGTAAAATGAAAAAATCACCATCGATGCCAGTAA
TCGCCGGTAAATTAAATGGCGAATCATTAAAATCACCAAATAAACCAAACCTTGCTAGTA
ATAATAATAATAATAATAACAACTATAGTAACAGTAATATAAGTTTTAGTAACATTAAAC
CAAACAATGGTAGTTTAAGTTCATCTGCCAACTCAATCTCCACTGGATTACCAAATTTAT
CAGGTGGCAAAATTAATACCAACGTTTACTCTTCACTTGGTAACATTGGAAGTGGAATGA
ATGGTTCTAGCACAAAGGATGATTCTGTTTCACCAACAAAGAAATCAACCGACCATCAAC
CAAGAAAACCAATGACCAAATCAGTATCAATGAGTTCAATTGGATTGAACAAXXXXXXXX
XXGTAACTACTTGATTCAATCATTTTTGAAACAATTTTCACCAGAGGTCAATCAATTTGT
TTGTGATGCAATCATGGGTAATATTGAGGAGATTTGTACCCATAAAGTTGGCTGTACAGT
CGTCAATCGTTGTATCGATAATGCCAATCCTGAGCAACTAGAGAAATTGGTCGATAAAAT
CACTGAACATTCCCTCAAATTGGTACAAGATCAATTTGGCAACTATGTCGTTCAACATCT
CCTCTCAAAGAATAAAGCTTACTCTTCAAAATTGATTGTCTCACTCAAAGATAACATTGC
TGAATTATCCATCCAAAAGTTTAGCTCCAATGTCATTGAAAAGTGTTTACAATTGGCTGA
TACTCCAACCTATGAATTGATCATCAAGGCTTTGACAGAAGCGTGATATCCTCAGTTTGC
TTCAAGATAAATATGCCAACTTCGTGATCCAAACCGCTTTGGACGTCAGTAACGATACTC
AACACGCAAAACTTGTAAAAATTATTGTACCCAACATTCATCAAATTAAGACTCCTTACG
AATTCACATTCAAAAGAAGAACTTCAAGTTAAAAAAGGATAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
APFKSPRKLNTSGSGLTSIIPPGFSNESSPNTSSNIVSPSTTTTTPLSSTSPSTTTTTTT
PTSFQPSGGKMKKSPSMPVIAGKLNGESLKSPNKPNLASNNNNNNNNYSNSNISFSNIKP
NNGSLSSSANSISTGLPNLSGGKINTNVYSSLGNIGSGMNGSSTKDDSVSPTKKSTDHQP
RKPMTKSVSMSSIGLN---

---NYLIQSFLKQFSPEVNQFVCDAIMGNIEEICTHKVGCTVVNRCIDNANPEQLEKLVD
KITEHSLKLVQDQFGNYVVQHLLSKNKAYSSKLIVSLKDNIAELSIQKFSSNVIEKCLQL
ADTPTYELIIKALTEA*ypqfasr*icqlrdpnrfgrq*rystrktcknyctqhssn*ds
lrihiqkknfklkkd
Translated Amino Acid sequence (All Frames) 
Frame A:
kphsnlqen*ihlvvd*lq*shqvfqtnlhqihqailylhqlqpphhyhqlhhqpqpqpl
hqhhsnqvevk*knhhrcq*spvn*manh*nhqinqtllviiiiiittivtvi*vlvtln
qtmvv*vhlptqspldyqiyqvakliptftlhlvtleve*mvlaqrmilfhqqrnqptin
qenq*pnqyq*vqld*t---

---vtt*fnhf*nnfhqrsinlfvmqswvilrrfvpiklavqssivvsimpilsn*rnws
ikslnipsnwykinlatmsfnissqrikltlqn*lshskitllnypskslapmslksvyn
wlilqpmn*ssrl*qkrdilsllqdkyanfviqtaldvsndtqhaklvkiivpnihqikt
pyeftfkrrtss*kri

Frame B:
spiqiskkikyiw*winfnnptrffkriftkyikqycisinynhhtiiinftinhnhnhy
tniiptkwr*nekitidasnrr*ikwriikitk*tkpc********ql**q*ykf**h*t
kqw*fkficqlnlhwitkfirwqn*yqrllftw*hwkwnewf*hkg*fcftnkeinrpst
kktndqisinefnwieq---

---*lldsiifetiftrgqsicl*cnhg*y*gdlyp*swlysrqslyr*cqs*atreigr
*nh*tfpqigtrsiwqlcrstsplke*sllfkidcltqr*hc*iihpkv*lqch*kvfti
g*ysnl*idhqgfdrsvissvcfkinmpts*skplwtsvtilntqnl*kllyptfiklrl
ltnshskeelqvkkg*

Frame C:
APFKSPRKLNTSGSGLTSIIPPGFSNESSPNTSSNIVSPSTTTTTPLSSTSPSTTTTTTT
PTSFQPSGGKMKKSPSMPVIAGKLNGESLKSPNKPNLASNNNNNNNNYSNSNISFSNIKP
NNGSLSSSANSISTGLPNLSGGKINTNVYSSLGNIGSGMNGSSTKDDSVSPTKKSTDHQP
RKPMTKSVSMSSIGLN---

---NYLIQSFLKQFSPEVNQFVCDAIMGNIEEICTHKVGCTVVNRCIDNANPEQLEKLVD
KITEHSLKLVQDQFGNYVVQHLLSKNKAYSSKLIVSLKDNIAELSIQKFSSNVIEKCLQL
ADTPTYELIIKALTEA*ypqfasr*icqlrdpnrfgrq*rystrktcknyctqhssn*ds
lrihiqkknfklkkd
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFJ353 (AFJ353Q) /CSM/AF/AFJ3-C/AFJ353Q.Seq.d/                       1651   0.0
SHA672 (SHA672Q) /CSM/SH/SHA6-C/SHA672Q.Seq.d/                       1045   0.0
SFA408 (SFA408Q) /CSM/SF/SFA4-A/SFA408Q.Seq.d/                        993   0.0
CHR212 (CHR212Q) /CSM/CH/CHR2-A/CHR212Q.Seq.d/                        763   0.0
CFB402 (CFB402Q) /CSM/CF/CFB4-A/CFB402Q.Seq.d/                        680   0.0
CHB837 (CHB837Q) /CSM/CH/CHB8-B/CHB837Q.Seq.d/                        303   3e-81
CHE817 (CHE817Q) /CSM/CH/CHE8-A/CHE817Q.Seq.d/                         74   5e-12
SSF609 (SSF609Q) /CSM/SS/SSF6-A/SSF609Q.Seq.d/                         38   0.30
SSC351 (SSC351Q) /CSM/SS/SSC3-C/SSC351Q.Seq.d/                         38   0.30
SLJ490 (SLJ490Q) /CSM/SL/SLJ4-D/SLJ490Q.Seq.d/                         38   0.30
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CF820078|CF820078.1 EST697460 Coccidioides posadasii saprobic phase cDNA library, 2 tosequence.    46   0.006  2
CG823696|CG823696.1 SOYDB11TH LargeInsertSoybeanGenLib Glycine max genomic clone H08H01:MTP9A22, genomic survey sequence.    36   0.33   2
AC114263|AC114263.2 Dictyostelium discoideum chromosome 2 map 215673-367476 strain AX4, complete sequence.    34   0.40   8
AL445564|AL445564.1 Mycoplasma pulmonis (strain UAB CTIP) complete genome; segment 2/3.    32   0.98   14
AC099543|AC099543.2 Homo sapiens chromosome 3 clone RP11-554B20, complete sequence.    38   1.0    7
AI314228|AI314228.1 uj35g11.x1 Sugano mouse kidney mkia Mus musculus cDNA clone IMAGE:1921988 3' similar to gb:X51942 Mouse mRNA for phenylalanine hydroxylase (MOUSE);, mRNA sequence.    32   1.2    3
AC006581|AC006581.16 Homo sapiens 12 BAC RP11-259O18 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    46   1.3    1
CD558489|CD558489.1 AGENCOURT_14477476 NIH_MGC_181 Homo sapiens cDNA clone IMAGE:30396626 5', mRNA sequence.    46   1.3    1
AC117452|AC117452.2 Homo sapiens 3 BAC RP11-341J3 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    34   1.7    9
AC123803|AC123803.4 Mus musculus chromosome 10 clone RP24-492I15, complete sequence.    42   2.9    5
dna update 2004. 9.25
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AE017343_431(AE017343|pid:none) Cryptococcus neoformans var. neo...   119   9e-30
(Q92359) RecName: Full=Pumilio domain-containing protein C6G9.14...   113   9e-30
CU638743_632(CU638743|pid:none) Podospora anserina genomic DNA c...   110   8e-28
CR954215_201(CR954215|pid:none) Ostreococcus tauri strain OTTH05...   112   1e-27
AP007151_730(AP007151|pid:none) Aspergillus oryzae RIB40 genomic...   111   4e-27
EF068153_1(EF068153|pid:none) Hortaea werneckii pumilio-family R...   109   3e-26
S57889_1(S57889|pid:none) YGL023=putative regulatory protein [Sa...   100   6e-25
CR380947_15(CR380947|pid:none) Candida glabrata strain CBS138 ch...    99   1e-24
AC021198_3(AC021198|pid:none) Sequence of BAC F14D7 from Arabido...   100   2e-24
DQ446324_1(DQ446324|pid:none) Arabidopsis thaliana clone pENTR22...   100   2e-24
protein update 2009. 4.23
PSORT 

 psg: 0.89  gvh: 0.41  alm: 0.47  top: 0.47  tms: 0.00  mit: 0.38  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: mitochondrial
	 28.0 %: cytoplasmic
	 20.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: endoplasmic reticulum
	  4.0 %: peroxisomal

>> prediction for AFJ353 is mit
5' end seq. ID AFJ353F
5' end seq. 
>AFJ353F.Seq
AAGCCCCATTCAAATCTCCAAGAAAATTAAATACATCTGGTAGTGGATTAACTTCAATAA
TCCCACCAGGTTTTTCAAACGAATCTTCACCAAATACATCAAGCAATATTGTATCTCCAT
CAACTACAACCACCACACCATTATCATCAACTTCACCATCAACCACAACCACAACCACTA
CACCAACATCATTCCAACCAAGTGGAGGTAAAATGAAAAAATCACCATCGATGCCAGTAA
TCGCCGGTAAATTAAATGGCGAATCATTAAAATCACCAAATAAACCAAACCTTGCTAGTA
ATAATAATAATAATAATAACAACTATAGTAACAGTAATATAAGTTTTAGTAACATTAAAC
CAAACAATGGTAGTTTAAGTTCATCTGCCAACTCAATCTCCACTGGATTACCAAATTTAT
CAGGTGGCAAAATTAATACCAACGTTTACTCTTCACTTGGTAACATTGGAAGTGGAATGA
ATGGTTCTAGCACAAAGGATGATTCTGTTTCACCAACAAAGAAATCAACCGACCATCAAC
CAAGAAAACCAATGACCAAATCAGTATCAATGAGTTCAATTGGATTGAACAA--------
--
Length of 5' end seq. 592
3' end seq. ID AFJ353Z
3' end seq. 
>AFJ353Z.Seq
----------GTAACTACTTGATTCAATCATTTTTGAAACAATTTTCACCAGAGGTCAAT
CAATTTGTTTGTGATGCAATCATGGGTAATATTGAGGAGATTTGTACCCATAAAGTTGGC
TGTACAGTCGTCAATCGTTGTATCGATAATGCCAATCCTGAGCAACTAGAGAAATTGGTC
GATAAAATCACTGAACATTCCCTCAAATTGGTACAAGATCAATTTGGCAACTATGTCGTT
CAACATCTCCTCTCAAAGAATAAAGCTTACTCTTCAAAATTGATTGTCTCACTCAAAGAT
AACATTGCTGAATTATCCATCCAAAAGTTTAGCTCCAATGTCATTGAAAAGTGTTTACAA
TTGGCTGATACTCCAACCTATGAATTGATCATCAAGGCTTTGACAGAAGCGTGATATCCT
CAGTTTGCTTCAAGATAAATATGCCAACTTCGTGATCCAAACCGCTTTGGACGTCAGTAA
CGATACTCAACACGCAAAACTTGTAAAAATTATTGTACCCAACATTCATCAAATTAAGAC
TCCTTACGAATTCACATTCAAAAGAAGAACTTCAAGTTAAAAAAGGATAA
Length of 3' end seq. 580
Connected seq. ID AFJ353P
Connected seq. 
>AFJ353P.Seq
AAGCCCCATTCAAATCTCCAAGAAAATTAAATACATCTGGTAGTGGATTAACTTCAATAA
TCCCACCAGGTTTTTCAAACGAATCTTCACCAAATACATCAAGCAATATTGTATCTCCAT
CAACTACAACCACCACACCATTATCATCAACTTCACCATCAACCACAACCACAACCACTA
CACCAACATCATTCCAACCAAGTGGAGGTAAAATGAAAAAATCACCATCGATGCCAGTAA
TCGCCGGTAAATTAAATGGCGAATCATTAAAATCACCAAATAAACCAAACCTTGCTAGTA
ATAATAATAATAATAATAACAACTATAGTAACAGTAATATAAGTTTTAGTAACATTAAAC
CAAACAATGGTAGTTTAAGTTCATCTGCCAACTCAATCTCCACTGGATTACCAAATTTAT
CAGGTGGCAAAATTAATACCAACGTTTACTCTTCACTTGGTAACATTGGAAGTGGAATGA
ATGGTTCTAGCACAAAGGATGATTCTGTTTCACCAACAAAGAAATCAACCGACCATCAAC
CAAGAAAACCAATGACCAAATCAGTATCAATGAGTTCAATTGGATTGAACAA--------
--GTAACTACTTGATTCAATCATTTTTGAAACAATTTTCACCAGAGGTCAATCAATTTGT
TTGTGATGCAATCATGGGTAATATTGAGGAGATTTGTACCCATAAAGTTGGCTGTACAGT
CGTCAATCGTTGTATCGATAATGCCAATCCTGAGCAACTAGAGAAATTGGTCGATAAAAT
CACTGAACATTCCCTCAAATTGGTACAAGATCAATTTGGCAACTATGTCGTTCAACATCT
CCTCTCAAAGAATAAAGCTTACTCTTCAAAATTGATTGTCTCACTCAAAGATAACATTGC
TGAATTATCCATCCAAAAGTTTAGCTCCAATGTCATTGAAAAGTGTTTACAATTGGCTGA
TACTCCAACCTATGAATTGATCATCAAGGCTTTGACAGAAGCGTGATATCCTCAGTTTGC
TTCAAGATAAATATGCCAACTTCGTGATCCAAACCGCTTTGGACGTCAGTAACGATACTC
AACACGCAAAACTTGTAAAAATTATTGTACCCAACATTCATCAAATTAAGACTCCTTACG
AATTCACATTCAAAAGAAGAACTTCAAGTTAAAAAAGGATAA
Length of connected seq. 1172
Full length Seq ID -
Full length Seq. -
Length of full length seq. -