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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHF505
Library SH
(Link to library)
Clone ID SHF505
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12067-1|Contig-U13261-1
Original site URL
Representative seq. ID SHF505P
(Link to Original site)
Representative DNA sequence 
>SHF505 (SHF505Q) /CSM/SH/SHF5-A/SHF505Q.Seq.d/
TTCAATTGTAATTGATAAGCTAGTGGATGCTTTATCAATTCCATCAGAGAGTGTTCAAGT
TGGTATTTCAAAATGTATCGCTCAATTGATTCCATCATTTAAGAAACAAGGTGATCGTTT
AATTCCAATGTTATTAGAGAAATTAAAGAATAGTAGTGGTAATTATGCAGATAGACGTGG
TGCAGCATTTGGTTTAGCAGGTTCTGTCAAGGGTTTAGGTATTGGTTCTTTGAAAAACTA
TTCAATTCTCGATACTCTTCAATCCTATATCGAAGATAAAAAACATCCAACTAGTCGTCA
AGGTGCTTTATTTGCTTTCGAATGTCTTTGTAATACAATTGGTCGTGTTTTCGAACCATA
CATTATTCATATTCTTCCAAAACTTTTAGTTTGTTTTGGTGATAATGTTAGTGAGGTTCG
TGATGCTACAGCAGATACTGCCAAAGCTATCATGTCACAACTTAGTGGTCATGGTGTTAA
AATCGTTTTACCAGCACTATTAAAAGCATTGGATGATCGTTCATGGAGAACCAAXXXXXX
XXXXTACACTCGATAAAGCAATCGCCACCATTGTGGACTTCCTTTATCAACNTTCCGATG
ATCTCAGATCAATGGTCGCTCAAACTATTGGTGCCTCTTCAAAATGTTTTACTTCTCTCA
CTGAATTGAATCAATTCATTAAAACCAATCTCATCTCACCTTCTCAATCAGTACTCTCTC
GTTATGGTAAATCATTGGCATTGGGTGAAATTTTCAAAGCATCTGGTAAGAATTTAATCG
ATTCTCAATCACCAAATATGCCAACCATCATTAAAATCATTCAAACCGATTGTCGTGATG
AAAAAGGTCCAATTCGTGAATCCTCTGCTTATTTGGCTGAAGCTATTTTAGTTGCTTCGC
CTTTAACCTATGCAAAGGATTTAGTACCTTCCATTTGTCATCTCATTGGTGATCAATCAT
CTTCCGTATCAATTAGTGCTCTCAATGTTATTAAACGTTTCTGTAAATCAAATCAACAAT
TATCAAGACAATATTTACGTGATATTGTCGTACCAACTATGAATAGATTAAAAGAAAGAA
CTAATTTACCTCTAAAATTAGCTGCTGAAAGAACTTTAGTTCATTCACTTCAAATCTTTA
AAGAATCCATTGTAATGGATGATTTAATTAAACAATTAGAATTATCTGGTGATTCATCAA
TGGCAAATTCTTTAATNATTATCATAAAAGAGTTTTAANAAATTA
sequence update 2002.10.25
Translated Amino Acid sequence 
SIVIDKLVDALSIPSESVQVGISKCIAQLIPSFKKQGDRLIPMLLEKLKNSSGNYADRRG
AAFGLAGSVKGLGIGSLKNYSILDTLQSYIEDKKHPTSRQGALFAFECLCNTIGRVFEPY
IIHILPKLLVCFGDNVSEVRDATADTAKAIMSQLSGHGVKIVLPALLKALDDRSWRT---

---TLDKAIATIVDFLYQXSDDLRSMVAQTIGASSKCFTSLTELNQFIKTNLISPSQSVL
SRYGKSLALGEIFKASGKNLIDSQSPNMPTIIKIIQTDCRDEKGPIRESSAYLAEAILVA
SPLTYAKDLVPSICHLIGDQSSSVSISALNVIKRFCKSNQQLSRQYLRDIVVPTMNRLKE
RTNLPLKLAAERTLVHSLQIFKESIVMDDLIKQLELSGDSSMANSLXIIIKEF*xi
Translated Amino Acid sequence (All Frames) 
Frame A:
fncn**asgcfinsirecsswyfkmyrsidsii*etr*sfnsnvireike**w*lcr*tw
csiwfsrfcqgfrywffeklfnsryssilyrr*ktsn*ssrcficfrmsl*ynwscfrti
hysyssktfslfw**c**gs*cysrycqsyhvtt*wswc*nrftstiksig*sfmenq--
-

---ytr*snrhhcglplstfr*sqingrsnywclfkmfyfsh*iesih*nqshltfsist
lslw*iigig*nfqsiw*efnrfsitkyanhh*nhsnrls**krsns*ilclfg*syfsc
fafnlckgfstfhlsshw*siifrin*csqcy*tfl*ikstiiktift*ycrtnye*ikr
kn*ftskisc*knfssftsnl*rihcng*fn*tiriiw*fingkffnxyhkrvlxn

Frame B:
SIVIDKLVDALSIPSESVQVGISKCIAQLIPSFKKQGDRLIPMLLEKLKNSSGNYADRRG
AAFGLAGSVKGLGIGSLKNYSILDTLQSYIEDKKHPTSRQGALFAFECLCNTIGRVFEPY
IIHILPKLLVCFGDNVSEVRDATADTAKAIMSQLSGHGVKIVLPALLKALDDRSWRT---

---TLDKAIATIVDFLYQXSDDLRSMVAQTIGASSKCFTSLTELNQFIKTNLISPSQSVL
SRYGKSLALGEIFKASGKNLIDSQSPNMPTIIKIIQTDCRDEKGPIRESSAYLAEAILVA
SPLTYAKDLVPSICHLIGDQSSSVSISALNVIKRFCKSNQQLSRQYLRDIVVPTMNRLKE
RTNLPLKLAAERTLVHSLQIFKESIVMDDLIKQLELSGDSSMANSLXIIIKEF*xi

Frame C:
ql*lis*wmlyqfhqrvfklvfqnvsln*fhhlrnkviv*fqcy*rn*rivvvimqidvv
qhlv*qvlsrv*vlvl*ktiqfsilfnpiskikniqlvvkvlyllsnvfviqlvvfsnht
lfiffqnf*fvlvimlvrfvmlqqilpklschnlvvmvlksfyqhy*khwmivhgep---

---hsikqspplwtsfinxpmisdqwslkllvplqnvlllsln*inslkpisshllnqys
lvmvnhwhwvkfskhlvri*silnhqicqpslksfkpivvmkkvqfvnplliwlklf*ll
rl*pmqri*ylpfvislvinhlpyqlvlsmllnvsvnqinnyqdniyvilsyql*id*kk
eliyl*n*llkel*fihfkslknpl*wmi*lnn*nylvihqwqil*xls*ksfxkl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHF505 (SHF505Q) /CSM/SH/SHF5-A/SHF505Q.Seq.d/                       2391   0.0
VHI374 (VHI374Q) /CSM/VH/VHI3-D/VHI374Q.Seq.d/                       1364   0.0
VHC675 (VHC675Q) /CSM/VH/VHC6-D/VHC675Q.Seq.d/                       1364   0.0
VHA441 (VHA441Q) /CSM/VH/VHA4-B/VHA441Q.Seq.d/                       1358   0.0
VHB245 (VHB245Q) /CSM/VH/VHB2-B/VHB245Q.Seq.d/                       1352   0.0
VHO589 (VHO589Q) /CSM/VH/VHO5-D/VHO589Q.Seq.d/                       1275   0.0
VHK689 (VHK689Q) /CSM/VH/VHK6-D/VHK689Q.Seq.d/                       1275   0.0
VHA755 (VHA755Q) /CSM/VH/VHA7-C/VHA755Q.Seq.d/                       1267   0.0
VHL277 (VHL277Q) /CSM/VH/VHL2-D/VHL277Q.Seq.d/                       1205   0.0
VHF215 (VHF215Q) /CSM/VH/VHF2-A/VHF215Q.Seq.d/                        957   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC119982|AC119982.6 Mus musculus chromosome 19, clone RP24-498L9, complete sequence.    56   0.002  1
AC118724|AC118724.18 Mus musculus, clone RP24-160H20, complete sequence.    56   0.002  1
BH167468|BH167468.1 ENTSH94TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    50   0.12   1
BH163191|BH163191.1 ENTTE33TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    50   0.12   1
AZ531303|AZ531303.1 ENTCH17TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    50   0.12   1
AQ947263|AQ947263.1 Sheared DNA-49E23.TR Sheared DNA Trypanosoma brucei genomic clone Sheared DNA-49E23, genomic survey sequence.    38   0.46   2
AQ651303|AQ651303.1 Sheared DNA-18P2.TR Sheared DNA Trypanosoma brucei genomic clone Sheared DNA-18P2, genomic survey sequence.    38   0.47   2
BX469894|BX469894.5 Zebrafish DNA sequence from clone CH211-212N23 in linkage group 14.    46   0.71   5
Z14092|Z14092.1 Caenorhabditis elegans Cosmid R107.                34   1.5    6
CT027722|CT027722.5 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-12H22.    46   1.9    1
dna update 2006. 3.17
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54WR2) RecName: Full=Translational activator gcn1; AltName: Fu...   423   e-117
AL954693_2(AL954693|pid:none) Zebrafish DNA sequence from clone ...   192   3e-47
BC168513_1(BC168513|pid:none) Danio rerio cDNA clone IMAGE:69620...   192   3e-47
AC004812_3(AC004812|pid:none) Homo sapiens PAC clone 267D11 from...   188   4e-46
U77700_1(U77700|pid:none) Homo sapiens HsGCN1 mRNA, partial cds.      188   4e-46
(Q99736) RecName: Full=Translational activator GCN1; AltName: Fu...   188   4e-46
D86973_1(D86973|pid:none) Homo sapiens mRNA for KIAA0219 gene, p...   188   4e-46
BC082548_1(BC082548|pid:none) Mus musculus GCN1 general control ...   187   5e-46
AB093217_1(AB093217|pid:none) Mus musculus mRNA for mKIAA0219 pr...   187   5e-46
AK147257_1(AK147257|pid:none) Mus musculus cDNA, RIKEN full-leng...   187   5e-46
protein update 2009. 4.18
PSORT 

 psg: 0.75  gvh: 0.44  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 28.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for SHF505 is cyt
5' end seq. ID SHF505F
5' end seq. 
>SHF505F.Seq
TTCAATTGTAATTGATAAGCTAGTGGATGCTTTATCAATTCCATCAGAGAGTGTTCAAGT
TGGTATTTCAAAATGTATCGCTCAATTGATTCCATCATTTAAGAAACAAGGTGATCGTTT
AATTCCAATGTTATTAGAGAAATTAAAGAATAGTAGTGGTAATTATGCAGATAGACGTGG
TGCAGCATTTGGTTTAGCAGGTTCTGTCAAGGGTTTAGGTATTGGTTCTTTGAAAAACTA
TTCAATTCTCGATACTCTTCAATCCTATATCGAAGATAAAAAACATCCAACTAGTCGTCA
AGGTGCTTTATTTGCTTTCGAATGTCTTTGTAATACAATTGGTCGTGTTTTCGAACCATA
CATTATTCATATTCTTCCAAAACTTTTAGTTTGTTTTGGTGATAATGTTAGTGAGGTTCG
TGATGCTACAGCAGATACTGCCAAAGCTATCATGTCACAACTTAGTGGTCATGGTGTTAA
AATCGTTTTACCAGCACTATTAAAAGCATTGGATGATCGTTCATGGAGAACCAANNNNNN
NNNN
Length of 5' end seq. 544
3' end seq. ID SHF505Z
3' end seq. 
>SHF505Z.Seq
NNNNNNNNNNTACACTCGATAAAGCAATCGCCACCATTGTGGACTTCCTTTATCAACNTT
CCGATGATCTCAGATCAATGGTCGCTCAAACTATTGGTGCCTCTTCAAAATGTTTTACTT
CTCTCACTGAATTGAATCAATTCATTAAAACCAATCTCATCTCACCTTCTCAATCAGTAC
TCTCTCGTTATGGTAAATCATTGGCATTGGGTGAAATTTTCAAAGCATCTGGTAAGAATT
TAATCGATTCTCAATCACCAAATATGCCAACCATCATTAAAATCATTCAAACCGATTGTC
GTGATGAAAAAGGTCCAATTCGTGAATCCTCTGCTTATTTGGCTGAAGCTATTTTAGTTG
CTTCGCCTTTAACCTATGCAAAGGATTTAGTACCTTCCATTTGTCATCTCATTGGTGATC
AATCATCTTCCGTATCAATTAGTGCTCTCAATGTTATTAAACGTTTCTGTAAATCAAATC
AACAATTATCAAGACAATATTTACGTGATATTGTCGTACCAACTATGAATAGATTAAAAG
AAAGAACTAATTTACCTCTAAAATTAGCTGCTGAAAGAACTTTAGTTCATTCACTTCAAA
TCTTTAAAGAATCCATTGTAATGGATGATTTAATTAAACAATTAGAATTATCTGGTGATT
CATCAATGGCAAATTCTTTAATNATTATCATAAAAGAGTTTTAANAAATTA
Length of 3' end seq. 711
Connected seq. ID SHF505P
Connected seq. 
>SHF505P.Seq
TTCAATTGTAATTGATAAGCTAGTGGATGCTTTATCAATTCCATCAGAGAGTGTTCAAGT
TGGTATTTCAAAATGTATCGCTCAATTGATTCCATCATTTAAGAAACAAGGTGATCGTTT
AATTCCAATGTTATTAGAGAAATTAAAGAATAGTAGTGGTAATTATGCAGATAGACGTGG
TGCAGCATTTGGTTTAGCAGGTTCTGTCAAGGGTTTAGGTATTGGTTCTTTGAAAAACTA
TTCAATTCTCGATACTCTTCAATCCTATATCGAAGATAAAAAACATCCAACTAGTCGTCA
AGGTGCTTTATTTGCTTTCGAATGTCTTTGTAATACAATTGGTCGTGTTTTCGAACCATA
CATTATTCATATTCTTCCAAAACTTTTAGTTTGTTTTGGTGATAATGTTAGTGAGGTTCG
TGATGCTACAGCAGATACTGCCAAAGCTATCATGTCACAACTTAGTGGTCATGGTGTTAA
AATCGTTTTACCAGCACTATTAAAAGCATTGGATGATCGTTCATGGAGAACCAA------
----TACACTCGATAAAGCAATCGCCACCATTGTGGACTTCCTTTATCAACNTTCCGATG
ATCTCAGATCAATGGTCGCTCAAACTATTGGTGCCTCTTCAAAATGTTTTACTTCTCTCA
CTGAATTGAATCAATTCATTAAAACCAATCTCATCTCACCTTCTCAATCAGTACTCTCTC
GTTATGGTAAATCATTGGCATTGGGTGAAATTTTCAAAGCATCTGGTAAGAATTTAATCG
ATTCTCAATCACCAAATATGCCAACCATCATTAAAATCATTCAAACCGATTGTCGTGATG
AAAAAGGTCCAATTCGTGAATCCTCTGCTTATTTGGCTGAAGCTATTTTAGTTGCTTCGC
CTTTAACCTATGCAAAGGATTTAGTACCTTCCATTTGTCATCTCATTGGTGATCAATCAT
CTTCCGTATCAATTAGTGCTCTCAATGTTATTAAACGTTTCTGTAAATCAAATCAACAAT
TATCAAGACAATATTTACGTGATATTGTCGTACCAACTATGAATAGATTAAAAGAAAGAA
CTAATTTACCTCTAAAATTAGCTGCTGAAAGAACTTTAGTTCATTCACTTCAAATCTTTA
AAGAATCCATTGTAATGGATGATTTAATTAAACAATTAGAATTATCTGGTGATTCATCAA
TGGCAAATTCTTTAATNATTATCATAAAAGAGTTTTAANAAATTA
Length of connected seq. 1235
Full length Seq ID -
Full length Seq. -
Length of full length seq. -