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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHF448
Library SH
(Link to library)
Clone ID SHF448
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11503-1
Original site URL
Representative seq. ID SHF448E
(Link to Original site)
Representative DNA sequence 
>SHF448 (SHF448Q) /CSM/SH/SHF4-B/SHF448Q.Seq.d/
ATCAAAAAAAACAGTTTTCTTTGTAAATTTATTAATATATGATTAGAAAATCAAATAATT
TTTCAATTTTGTTTGCAATATTTTTAAAAATTGTTTTTGTGGTTTCAGCGCCGCTATGCC
CAAATTCAACGATTTTATTAAATTACAACATCCTAACGGTTTATAATTCATCAGAAGGAT
GTGGTTTTAATAACGAGCCGATATGCACATCACTCAAAGATGCTGTTAGCAGAGCATTTT
TATTAATATCAAATAATAGTCGTGTTTGTATTGGTATCATTGGTAATATAAATGTAACAT
CAGAACAAATTACATTGGGTAATTATTGTGGTGCCTTGTGGATCACTTCAGAAAATATCA
ATAATGAAAATAATAATTATNCAATAATCGATGGATCAAGCTCAACCCAACCATTTNTGA
CATATGAACCAGAAGAAGAAAATAACCAAACTCAATCATGTTCAAGATGGNTCTTCTTTC
AATATATGAATTTCACAAGTTGGGATTGGAAATAATTCAGATGTGAATTTATCAAAAAAA
AGCNNTTAAATTAAATAACATTTATTGTGGTTGTGAAGAATGTAAATATTTTCTTATATA
TGGTTCTCAATTTATTCCCATCGTTAACCACACTTGCATTGATCCGACACTGACACCGAC
ACCAACAACTTCAACAACATCTACTACATTTGCTTCCACAACATCAACCACAACTACTTC
AACAACATCAAACGCATCAGCTGGAACAACATCAACTACTTCAGCTGCAACAACACCAAC
CACATCAGCTTTAACAACATCAACTACAACTACAACATCAACTACAACAACTATAACTAC
NTCAACTACATTACCATCAACATCAACAACAACAAGTCCAATTGATAGTAAGANTAGGAA
TATTATCATTGTTACAGTTATTTTGGGCTTGATCTATAGCATGTGGTAGACTATGAACGT
CAATTTGAAGATTATGCGGATAGGGTTAACTCAACTTCANATCTCATATCGTTTTTAC
sequence update 2002.10.27
Translated Amino Acid sequence 
qkkqfsl*iy*YMIRKSNNFSILFAIFLKIVFVVSAPLCPNSTILLNYNILTVYNSSEGC
GFNNEPICTSLKDAVSRAFLLISNNSRVCIGIIGNINVTSEQITLGNYCGALWITSENIN
NENNNYXIIDGSSSTQPFXTYEPEEENNQTQSCSRWXFFQYMNFTSWDWK*frcefikkk
xln*itfivvvknvniflymvlnlfpslttlalirh*hrhqqlqqhllhllpqhqpqllq
qhqthqleqhqllqlqqhqphql*qhqlqlqhqlqql*lxqlhyhqhqqqqvqlivrxgi
lsllqlfwa*siacgrl*tsi*rlcg*g*lnfxshivf
Translated Amino Acid sequence (All Frames) 
Frame A:
ikknsflckfini*lenqiifqfclqyf*klflwfqrryaqiqrfy*itts*rfiihqkd
vvlitsryahhskmllaehfy*yqiivvfvlvslvi*m*hqnklhwviivvpcgslqkis
imkiiixq*smdqaqpnhx*hmnqkkkitklnhvqdgssfni*isqvgignnsdvnlskk
sx*ik*hllwl*rm*ifsyiwfsiyshr*phlh*sdtdtdtnnfnniyyicfhninhnyf
nnikriswnninyfscnntnhisfnninynyninynnynyxnyitininnnksn***x*e
yyhcysyfgldl*hvvdyerqfedyadrvnstsxlisfl


Frame B:
skktvffvnlliyd*kik*ffnfvcnifkncfcgfsaampkfndfiklqhpngl*firrm
wf**radmhitqrcc*qsifinik**sclywyhw*ykcnirtnyig*llwclvdhfrkyq
**k**lxnnrwiklnptixdi*trrrk*pnsimfkmxllsiyefhklgleiiqm*iyqkk
axklnniycgceeckyfliygsqfipivnhtcidptltptpttsttsttfasttstttts
ttsnasagttsttsaattpttsalttstttttsttttittsttlpststttspidskxrn
iiivtvilgliysmw*tmnvnlkimrigltqlxisyrfy


Frame C:
qkkqfsl*iy*YMIRKSNNFSILFAIFLKIVFVVSAPLCPNSTILLNYNILTVYNSSEGC
GFNNEPICTSLKDAVSRAFLLISNNSRVCIGIIGNINVTSEQITLGNYCGALWITSENIN
NENNNYXIIDGSSSTQPFXTYEPEEENNQTQSCSRWXFFQYMNFTSWDWK*frcefikkk
xln*itfivvvknvniflymvlnlfpslttlalirh*hrhqqlqqhllhllpqhqpqllq
qhqthqleqhqllqlqqhqphql*qhqlqlqhqlqql*lxqlhyhqhqqqqvqlivrxgi
lsllqlfwa*siacgrl*tsi*rlcg*g*lnfxshivf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHF448 (SHF448Q) /CSM/SH/SHF4-B/SHF448Q.Seq.d/                       1231   0.0
VHE495 (VHE495Q) /CSM/VH/VHE4-D/VHE495Q.Seq.d/                       1207   0.0
SHD533 (SHD533Q) /CSM/SH/SHD5-B/SHD533Q.Seq.d/                       1207   0.0
AHK839 (AHK839Q) /CSM/AH/AHK8-B/AHK839Q.Seq.d/                       1207   0.0
AHG336 (AHG336Q) /CSM/AH/AHG3-B/AHG336Q.Seq.d/                       1207   0.0
AHF753 (AHF753Q) /CSM/AH/AHF7-C/AHF753Q.Seq.d/                       1207   0.0
AHA119 (AHA119Q) /CSM/AH/AHA1-A/AHA119Q.Seq.d/                       1207   0.0
SHC657 (SHC657Q) /CSM/SH/SHC6-C/SHC657Q.Seq.d/                       1201   0.0
AHL573 (AHL573Q) /CSM/AH/AHL5-D/AHL573Q.Seq.d/                       1201   0.0
AHK570 (AHK570Q) /CSM/AH/AHK5-C/AHK570Q.Seq.d/                       1201   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC115680|AC115680.3 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.    36   5e-07  3
CV428924|CV428924.1 EST02153 Atlantic salmon Lambda Zap Express heart cDNA library Salmo salar cDNA clone hr_004_h06 5' similar to desmin, mRNA sequence.    44   2e-05  3
CB037667|CB037667.1 Tc_ad2_36G07_TEXF1 Teladorsagia circumcincta adults library 2 Teladorsagia circumcincta cDNA clone Tc_ad2_36G07 5', mRNA sequence.    48   1e-04  2
DQ121075|DQ121075.1 Hucho taimen clone ZA-69 microsatellite sequence.    36   0.004  4
DQ121097|DQ121097.1 Hucho taimen clone ZA-9 microsatellite sequence.    36   0.004  4
BX005250|BX005250.7 Zebrafish DNA sequence from clone DKEY-35H6 in linkage group 14.    44   0.004  2
U85395|U85395.1 Onchocerca volvulus Gln-rich protein mRNA, complete cds.    38   0.005  2
CK882927|CK882927.1 SGP147615 Atlantic salmon Heart cDNA library Salmo salar cDNA clone H6-0590, mRNA sequence.    34   0.006  3
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    36   0.009  7
AL929353|AL929353.1 Plasmodium falciparum strain 3D7, chromosome 5, segment 3/4.    36   0.011  11
dna update 2006. 3.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF020283_1(AF020283|pid:none) Dictyostelium discoideum DG2044 ge...    60   1e-07
CP000766_763(CP000766|pid:none) Rickettsia rickettsii str. Iowa,...    37   1.3
AE006914_723(AE006914|pid:none) Rickettsia conorii str. Malish 7...    35   5.1
protein update 2009. 4.18
PSORT 

 psg: 0.99  gvh: 0.48  alm: 0.21  top: 0.27  tms: 0.07  mit: 0.47  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.45  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 48.0 %: mitochondrial
	 20.0 %: Golgi
	 12.0 %: nuclear
	  8.0 %: endoplasmic reticulum
	  4.0 %: cytoplasmic
	  4.0 %: vacuolar
	  4.0 %: extracellular, including cell wall

>> prediction for SHF448 is mit
5' end seq. ID SHF448F
5' end seq. 
>SHF448F.Seq
ATCAAAAAAAACAGTTTTCTTTGTAAATTTATTAATATATGATTAGAAAATCAAATAATT
TTTCAATTTTGTTTGCAATATTTTTAAAAATTGTTTTTGTGGTTTCAGCGCCGCTATGCC
CAAATTCAACGATTTTATTAAATTACAACATCCTAACGGTTTATAATTCATCAGAAGGAT
GTGGTTTTAATAACGAGCCGATATGCACATCACTCAAAGATGCTGTTAGCAGAGCATTTT
TATTAATATCAAATAATAGTCGTGTTTGTATTGGTATCATTGGTAATATAAATGTAACAT
CAGAACAAATTACATTGGGTAATTATTGTGGTGCCTTGTGGATCACTTCAGAAAATATCA
ATAATGAAAATAATAATTATACAATAATCGATGGATCAAGCTCAACCCANCCNNNNNNNN
NN
Length of 5' end seq. 422
3' end seq. ID SHF448Z
3' end seq. 
>SHF448Z.Seq
NNNNNNNNNNATCACTTCAGAAAATATCAATAATGAAAATAATAATTATCCAATAATCGA
GGATCAAGCTCAACCCAACCATTTNTGACATATGAACCAGAAGAAGAAAATAACCAAACT
CAATCATGTTCAAGATGGNTCTTCTTTCAATATATGAATTTCACAAGTTGGGATTGGAAA
TAATTCAGATGTGAATTTATCAAAAAAAAGCNNTTAAATTAAATAACATTTATTGTGGTT
GTGAAGAATGTAAATATTTTCTTATATATGGTTCTCAATTTATTCCCATCGTTAACCACA
CTTGCATTGATCCGACACTGACACCGACACCAACAACTTCAACAACATCTACTACATTTG
CTTCCACAACATCAACCACAACTACTTCAACAACATCAAACGCATCAGCTGGAACAACAT
CAACTACTTCAGCTGCAACAACACCAACCACATCAGCTTTAACAACATCAACTACAACTA
CAACATCAACTACAACAACTATAACTACNTCAACTACATTACCATCAACATCAACAACAA
CAAGTCCAATTGATAGTAAGANTAGGAATATTATCATTGTTACAGTTATTTTGGGCTTGA
TCTATAGCATGTGGTAGACTATGAACGTCAATTTGAAGATTATGCGGATAGGGTTAACTC
AACTTCANATCTCATATCGTTTTTAC
Length of 3' end seq. 686
Connected seq. ID SHF448P
Connected seq. 
>SHF448P.Seq
ATCAAAAAAAACAGTTTTCTTTGTAAATTTATTAATATATGATTAGAAAATCAAATAATT
TTTCAATTTTGTTTGCAATATTTTTAAAAATTGTTTTTGTGGTTTCAGCGCCGCTATGCC
CAAATTCAACGATTTTATTAAATTACAACATCCTAACGGTTTATAATTCATCAGAAGGAT
GTGGTTTTAATAACGAGCCGATATGCACATCACTCAAAGATGCTGTTAGCAGAGCATTTT
TATTAATATCAAATAATAGTCGTGTTTGTATTGGTATCATTGGTAATATAAATGTAACAT
CAGAACAAATTACATTGGGTAATTATTGTGGTGCCTTGTGGATCACTTCAGAAAATATCA
ATAATGAAAATAATAATTATACAATAATCGATGGATCAAGCTCAACCCANCC--------
--ATCACTTCAGAAAATATCAATAATGAAAATAATAATTATCCAATAATCGAGGATCAAG
CTCAACCCAACCATTTNTGACATATGAACCAGAAGAAGAAAATAACCAAACTCAATCATG
TTCAAGATGGNTCTTCTTTCAATATATGAATTTCACAAGTTGGGATTGGAAATAATTCAG
ATGTGAATTTATCAAAAAAAAGCNNTTAAATTAAATAACATTTATTGTGGTTGTGAAGAA
TGTAAATATTTTCTTATATATGGTTCTCAATTTATTCCCATCGTTAACCACACTTGCATT
GATCCGACACTGACACCGACACCAACAACTTCAACAACATCTACTACATTTGCTTCCACA
ACATCAACCACAACTACTTCAACAACATCAAACGCATCAGCTGGAACAACATCAACTACT
TCAGCTGCAACAACACCAACCACATCAGCTTTAACAACATCAACTACAACTACAACATCA
ACTACAACAACTATAACTACNTCAACTACATTACCATCAACATCAACAACAACAAGTCCA
ATTGATAGTAAGANTAGGAATATTATCATTGTTACAGTTATTTTGGGCTTGATCTATAGC
ATGTGGTAGACTATGAACGTCAATTTGAAGATTATGCGGATAGGGTTAACTCAACTTCAN
ATCTCATATCGTTTTTAC
Length of connected seq. 1088
Full length Seq ID SHF448E
Full length Seq. 
>SHF448E.Seq
ATCAAAAAAAACAGTTTTCTTTGTAAATTTATTAATATATGATTAGAAAATCAAATAATT
TTTCAATTTTGTTTGCAATATTTTTAAAAATTGTTTTTGTGGTTTCAGCGCCGCTATGCC
CAAATTCAACGATTTTATTAAATTACAACATCCTAACGGTTTATAATTCATCAGAAGGAT
GTGGTTTTAATAACGAGCCGATATGCACATCACTCAAAGATGCTGTTAGCAGAGCATTTT
TATTAATATCAAATAATAGTCGTGTTTGTATTGGTATCATTGGTAATATAAATGTAACAT
CAGAACAAATTACATTGGGTAATTATTGTGGTGCCTTGTGGATCACTTCAGAAAATATCA
ATAATGAAAATAATAATTATNCAATAATCGATGGATCAAGCTCAACCCAACCATTTNTGA
CATATGAACCAGAAGAAGAAAATAACCAAACTCAATCATGTTCAAGATGGNTCTTCTTTC
AATATATGAATTTCACAAGTTGGGATTGGAAATAATTCAGATGTGAATTTATCAAAAAAA
AGCNNTTAAATTAAATAACATTTATTGTGGTTGTGAAGAATGTAAATATTTTCTTATATA
TGGTTCTCAATTTATTCCCATCGTTAACCACACTTGCATTGATCCGACACTGACACCGAC
ACCAACAACTTCAACAACATCTACTACATTTGCTTCCACAACATCAACCACAACTACTTC
AACAACATCAAACGCATCAGCTGGAACAACATCAACTACTTCAGCTGCAACAACACCAAC
CACATCAGCTTTAACAACATCAACTACAACTACAACATCAACTACAACAACTATAACTAC
NTCAACTACATTACCATCAACATCAACAACAACAAGTCCAATTGATAGTAAGANTAGGAA
TATTATCATTGTTACAGTTATTTTGGGCTTGATCTATAGCATGTGGTAGACTATGAACGT
CAATTTGAAGATTATGCGGATAGGGTTAACTCAACTTCANATCTCATATCGTTTTTAC
Length of full length seq. 1018