>SHF432 (SHF432Q) /CSM/SH/SHF4-B/SHF432Q.Seq.d/
ATNAATTATTATATTTTAATATATAATAATATTTAATATATTTAATATATTAAATATATT
ATCAAAATTCAATCAAATTTGAATAATATAAAATAAATTATTTATTTATTTTTTTTATTT
ATTAAATTATTTGTGGGATTTGCAAATAATTCAAAATGGAAATTGAGCCTGTTGTAAGAA
TATCTTTTAATGGAAATAATAATCAAAACAACAACAACAACAACAACAACAACACCAATA
ATAATAGTAACAACAATAATAATAACAATAATAGTAGTAACATTAATAGCACCAATAAAC
CTAGTGTAAAAAAACCAATAAATCAAAAAATGATATCAAATATTAATAATCAAAAATCAC
CAAATCCATTAAATTCAAGTGTTGATGATAATAATAATACTAATAATAATAACAATAGTA
ATAGTAATGGAACAGATGAAGATATTATAAAATTAGCTAGAGATTTAACCAATACATTTG
GTATATTATTAGGGGATTCTGATCAACCATCGCAGTTTAGTGATGTAATATTTAAAGTTG
GTGATAGGAAATTCTTTGCAAGTAAGATAATGTTAGTGGCAAGAAGCGAGTACTTTAAAG
CAATGTTCACTGGATCAATGAAAGAATCAXXXXXXXXXXCGATAAGAATTTATTGGATGA
AGTATTACCACTAATTCGTTGGGAAAATATGAATATCGGCGAAGCATTTGAACGTTTAGA
TAATTTAAAAATTATACCAGATAAAGAAATTTCAAATCTTCTTAGACATATTTTAAAATC
AAATAGAGGTGAAAGTTTCTCATTTTTAGGTTACCATTATAGAAGACCACGTAATAATAG
AAAAAGACCCTATCCAATTGAATTTTTATTAGGTATTACTCAACCATCTGATTCAAATGT
TACAAATGAAATTATACCATTAAATTCATTTTCATCAGAATTTGGTCAATCAACTAATCA
ATTTTTATATAGAATTAAAAAGGATATAAAATTACCAGCAAATTTAGAGAGATTCTCATT
CAAGGATAGAGAGTTTTTCGTTCAATTAAAGGAAAGAGAAAAAGGTCAATTGGCTGTTTA
TTTGGCATTTGGTAGTAAGTTAACAAAACCATTAGCAATATCAGTTTTGGCAAGTGTAAT
TGGGTTTAGATTCCAAGATTCAATTGAATTTAGTTTTAAGAAAATGTTTGATGAAGGATT
CGATAGTGCTTGGGGTTGGCCAAAATTCATCTCTTTAGATACTTTATATAAACAAGATAA
ATATAGTCATTATTCTG

xllyfni**yliyliy*iyyqnsikfe*ykinylfiffiy*iicgick*FKMEIEPVVRI
SFNGNNNQNNNNNNNNNTNNNSNNNNNNNNSSNINSTNKPSVKKPINQKMISNINNQKSP
NPLNSSVDDNNNTNNNNNSNSNGTDEDIIKLARDLTNTFGILLGDSDQPSQFSDVIFKVG
DRKFFASKIMLVARSEYFKAMFTGSMKES---

---DKNLLDEVLPLIRWENMNIGEAFERLDNLKIIPDKEISNLLRHILKSNRGESFSFLG
YHYRRPRNNRKRPYPIEFLLGITQPSDSNVTNEIIPLNSFSSEFGQSTNQFLYRIKKDIK
LPANLERFSFKDREFFVQLKEREKGQLAVYLAFGSKLTKPLAISVLASVIGFRFQDSIEF
SFKKMFDEGFDSAWGWPKFISLDTLYKQDKYSHYS

Frame A:
xnyyiliynni*yi*yikyiikiqsnlnnik*iiylfflfiklfvgfannskwklsll*e
yllmeiiiktttttttttpiiivttiiitiivvtliapinlv*knq*ikk*yqiliiknh
qih*iqvlmiiiiliiitivivmeqmkil*n*lei*pihlvyy*gilinhrslvm*ylkl
vignslqvr*c*wqeastlkqcsldq*kn---

---r*efig*sittnslgkyeyrrsi*tfr*fknytr*rnfkss*tyfkik*r*kflifr
lpl*ktt***kktlsn*ifiryysti*fkcyk*nytikfifiriwsin*sifi*n*kgyk
itskfreiliqg*rvfrsikgkrkrsigclfgiw**vnktisnisfgkcnwv*iprfn*i
*f*env**rir*clglakihlfryfi*tr*i*slf

Frame B:
xiiif*yiiifnifnilnilskfnqi*ii*nklfiyffyllnylwdlqiiqngn*acckn
if*wk**skqqqqqqqqhq****qq***q****h**hq*t*ckktnkskndiky**skit
ksikfkc*****y****q****wnr*ryykis*rfnqyiwyiirgf*stiav**cni*sw
**eilck*dnvsgkkrvl*snvhwineri---

---DKNLLDEVLPLIRWENMNIGEAFERLDNLKIIPDKEISNLLRHILKSNRGESFSFLG
YHYRRPRNNRKRPYPIEFLLGITQPSDSNVTNEIIPLNSFSSEFGQSTNQFLYRIKKDIK
LPANLERFSFKDREFFVQLKEREKGQLAVYLAFGSKLTKPLAISVLASVIGFRFQDSIEF
SFKKMFDEGFDSAWGWPKFISLDTLYKQDKYSHYS

Frame C:
xllyfni**yliyliy*iyyqnsikfe*ykinylfiffiy*iicgick*FKMEIEPVVRI
SFNGNNNQNNNNNNNNNTNNNSNNNNNNNNSSNINSTNKPSVKKPINQKMISNINNQKSP
NPLNSSVDDNNNTNNNNNSNSNGTDEDIIKLARDLTNTFGILLGDSDQPSQFSDVIFKVG
DRKFFASKIMLVARSEYFKAMFTGSMKES---

---iriywmkyyh*fvgki*isakhlnv*ii*klyqikkfqifldif*nqievkvshf*v
tiiedhviiekdpiqlnfy*vllnhliqmlqmklyh*ihfhqnlvnqlinfyielkri*n
yqqi*rdshsriesfsfn*rkekkvnwlfiwhlvvs*qnh*qyqfwqv*lgldskiqlnl
vlrkclmkdsivlgvgqnssl*ilyinkiniviil

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHF432 (SHF432Q) /CSM/SH/SHF4-B/SHF432Q.Seq.d/                       1624   0.0
CHQ338 (CHQ338Q) /CSM/CH/CHQ3-B/CHQ338Q.Seq.d/                       1257   0.0
CHI268 (CHI268Q) /CSM/CH/CHI2-C/CHI268Q.Seq.d/                       1249   0.0
CHO487 (CHO487Q) /CSM/CH/CHO4-D/CHO487Q.Seq.d/                       1247   0.0
CHI868 (CHI868Q) /CSM/CH/CHI8-C/CHI868Q.Seq.d/                       1207   0.0
CHO649 (CHO649Q) /CSM/CH/CHO6-C/CHO649Q.Seq.d/                       1140   0.0
SSK328 (SSK328Q) /CSM/SS/SSK3-B/SSK328Q.Seq.d/                       1047   0.0
SSF262 (SSF262Q) /CSM/SS/SSF2-C/SSF262Q.Seq.d/                       1047   0.0
CHF780 (CHF780Q) /CSM/CH/CHF7-D/CHF780Q.Seq.d/                       1035   0.0
CHK429 (CHK429Q) /CSM/CH/CHK4-B/CHK429Q.Seq.d/                        942   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC139787|AC139787.1 Homo sapiens chromosome 5 clone RP11-1149K19, WORKING DRAFT SEQUENCE.    36   0.010  7
AL728302|AL728302.1 Danio rerio embryonic inner ear subtracted cDNA library 3PRIM end of clone BN0AA098ZG03 of Danio rerio.    44   0.021  2
AC115612|AC115612.2 Dictyostelium discoideum chromosome 2 map 6245135-6357017 strain AX4, complete sequence.    38   0.052  7
AC140141|AC140141.1 Homo sapiens chromosome 5 clone RP11-1422L3, WORKING DRAFT SEQUENCE, 1 unordered piece.    36   0.057  8
AC144584|AC144584.1 Homo sapiens chromosome 5 clone RP11-1053P23, WORKING DRAFT SEQUENCE, 2 unordered pieces.    36   0.070  7
AC091956|AC091956.3 Homo sapiens chromosome 5 clone RP11-430F16, complete sequence.    36   0.11   7
CD298939|CD298939.1 AGENCOURT_14249832 NIH_MGC_187 Homo sapiens cDNA clone IMAGE:30401391 5', mRNA sequence.    44   0.14   2
AC139285|AC139285.2 Homo sapiens chromosome 5 clone RP11-844O3, WORKING DRAFT SEQUENCE, 3 unordered pieces.    36   0.14   8
AE014824|AE014824.1 Plasmodium falciparum 3D7 chromosome 14 section 9 of 13 of the complete sequence.    34   0.15   13
CP000123|CP000123.1 Mycoplasma capricolum subsp. capricolum ATCC 27343, complete genome.    38   0.46   17

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AB027612_1(AB027612|pid:none) Drosophila melanogaster mRNA for k...    45   0.005
EU344973_1(EU344973|pid:none) Zea mays MAB1 gene, complete cds.        45   0.005
AK013278_1(AK013278|pid:none) Mus musculus 10, 11 days embryo wh...    45   0.007
(Q9CR40) RecName: Full=Kelch-like protein 28; AltName: Full=BTB/...    45   0.007
AK289855_1(AK289855|pid:none) Homo sapiens cDNA FLJ76338 complet...    45   0.007
BC146233_1(BC146233|pid:none) Bos taurus kelch-like 28 (Drosophi...    45   0.007
AK000088_1(AK000088|pid:none) Homo sapiens cDNA FLJ20081 fis, cl...    45   0.007
AB179382_1(AB179382|pid:none) Macaca fascicularis testis cDNA cl...    45   0.007
AP004183_11(AP004183|pid:none) Oryza sativa Japonica Group genom...    45   0.007
T16939(T16939)hypothetical protein T25D10.1 - Caenorhabditis ele...    44   0.012

 psg: 0.64  gvh: 0.11  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.10  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: nuclear
	 32.0 %: cytoplasmic
	  4.0 %: vacuolar
	  4.0 %: Golgi

>> prediction for SHF432 is nuc

>SHF432F.Seq
ATNAATTATTATATTTTAATATATAATAATATTTAATATATTTAATATATTAAATATATT
ATCAAAATTCAATCAAATTTGAATAATATAAAATAAATTATTTATTTATTTTTTTTATTT
ATTAAATTATTTGTGGGATTTGCAAATAATTCAAAATGGAAATTGAGCCTGTTGTAAGAA
TATCTTTTAATGGAAATAATAATCAAAACAACAACAACAACAACAACAACAACACCAATA
ATAATAGTAACAACAATAATAATAACAATAATAGTAGTAACATTAATAGCACCAATAAAC
CTAGTGTAAAAAAACCAATAAATCAAAAAATGATATCAAATATTAATAATCAAAAATCAC
CAAATCCATTAAATTCAAGTGTTGATGATAATAATAATACTAATAATAATAACAATAGTA
ATAGTAATGGAACAGATGAAGATATTATAAAATTAGCTAGAGATTTAACCAATACATTTG
GTATATTATTAGGGGATTCTGATCAACCATCGCAGTTTAGTGATGTAATATTTAAAGTTG
GTGATAGGAAATTCTTTGCAAGTAAGATAATGTTAGTGGCAAGAAGCGAGTACTTTAAAG
CAATGTTCACTGGATCAATGAAAGAATCANNNNNNNNNN

>SHF432Z.Seq
NNNNNNNNNNCGATAAGAATTTATTGGATGAAGTATTACCACTAATTCGTTGGGAAAATA
TGAATATCGGCGAAGCATTTGAACGTTTAGATAATTTAAAAATTATACCAGATAAAGAAA
TTTCAAATCTTCTTAGACATATTTTAAAATCAAATAGAGGTGAAAGTTTCTCATTTTTAG
GTTACCATTATAGAAGACCACGTAATAATAGAAAAAGACCCTATCCAATTGAATTTTTAT
TAGGTATTACTCAACCATCTGATTCAAATGTTACAAATGAAATTATACCATTAAATTCAT
TTTCATCAGAATTTGGTCAATCAACTAATCAATTTTTATATAGAATTAAAAAGGATATAA
AATTACCAGCAAATTTAGAGAGATTCTCATTCAAGGATAGAGAGTTTTTCGTTCAATTAA
AGGAAAGAGAAAAAGGTCAATTGGCTGTTTATTTGGCATTTGGTAGTAAGTTAACAAAAC
CATTAGCAATATCAGTTTTGGCAAGTGTAATTGGGTTTAGATTCCAAGATTCAATTGAAT
TTAGTTTTAAGAAAATGTTTGATGAAGGATTCGATAGTGCTTGGGGTTGGCCAAAATTCA
TCTCTTTAGATACTTTATATAAACAAGATAAATATAGTCATTATTCTG

>SHF432P.Seq
ATNAATTATTATATTTTAATATATAATAATATTTAATATATTTAATATATTAAATATATT
ATCAAAATTCAATCAAATTTGAATAATATAAAATAAATTATTTATTTATTTTTTTTATTT
ATTAAATTATTTGTGGGATTTGCAAATAATTCAAAATGGAAATTGAGCCTGTTGTAAGAA
TATCTTTTAATGGAAATAATAATCAAAACAACAACAACAACAACAACAACAACACCAATA
ATAATAGTAACAACAATAATAATAACAATAATAGTAGTAACATTAATAGCACCAATAAAC
CTAGTGTAAAAAAACCAATAAATCAAAAAATGATATCAAATATTAATAATCAAAAATCAC
CAAATCCATTAAATTCAAGTGTTGATGATAATAATAATACTAATAATAATAACAATAGTA
ATAGTAATGGAACAGATGAAGATATTATAAAATTAGCTAGAGATTTAACCAATACATTTG
GTATATTATTAGGGGATTCTGATCAACCATCGCAGTTTAGTGATGTAATATTTAAAGTTG
GTGATAGGAAATTCTTTGCAAGTAAGATAATGTTAGTGGCAAGAAGCGAGTACTTTAAAG
CAATGTTCACTGGATCAATGAAAGAATCA----------CGATAAGAATTTATTGGATGA
AGTATTACCACTAATTCGTTGGGAAAATATGAATATCGGCGAAGCATTTGAACGTTTAGA
TAATTTAAAAATTATACCAGATAAAGAAATTTCAAATCTTCTTAGACATATTTTAAAATC
AAATAGAGGTGAAAGTTTCTCATTTTTAGGTTACCATTATAGAAGACCACGTAATAATAG
AAAAAGACCCTATCCAATTGAATTTTTATTAGGTATTACTCAACCATCTGATTCAAATGT
TACAAATGAAATTATACCATTAAATTCATTTTCATCAGAATTTGGTCAATCAACTAATCA
ATTTTTATATAGAATTAAAAAGGATATAAAATTACCAGCAAATTTAGAGAGATTCTCATT
CAAGGATAGAGAGTTTTTCGTTCAATTAAAGGAAAGAGAAAAAGGTCAATTGGCTGTTTA
TTTGGCATTTGGTAGTAAGTTAACAAAACCATTAGCAATATCAGTTTTGGCAAGTGTAAT
TGGGTTTAGATTCCAAGATTCAATTGAATTTAGTTTTAAGAAAATGTTTGATGAAGGATT
CGATAGTGCTTGGGGTTGGCCAAAATTCATCTCTTTAGATACTTTATATAAACAAGATAA
ATATAGTCATTATTCTG