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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHF410
Library SH
(Link to library)
Clone ID SHF410
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13024-1|Contig-U16344-1
Original site URL
Representative seq. ID SHF410P
(Link to Original site)
Representative DNA sequence 
>SHF410 (SHF410Q) /CSM/SH/SHF4-A/SHF410Q.Seq.d/
ATTCAATCAAACAAACAAAAAACAACAATTTAAAAAAACAATAATTTATAGTAAAGTAAA
AACAATTAAAGAATCACTAATTGTTTTTTTCTCCTCCTCACTATAAATTCCAATATTTTT
TTTTAAATTAATTTCCAGCTTTTCTGTTTTTTTACACAATCACAACCAACCCTATATCAT
TACTACCAACCCTATACCATTACAATCTTATTTATTAAATAATTATTATTTGTTGTATTA
TTTTAGTGTATATTTGTTGATTTAATATTTATTAGCAAGTAACAAACAAAAAAAAAAXXX
XXXXXXXTGGTCGCTGGTGGTTCAATTGAATCAAAAGTTATTGATTTCACTCATGGTACC
ATTCAAGGTGTTGGTGNTCTTAAATGTGGTACTTGTAACTTTGCCAATAAGATCGCTGCT
AACNTCAACATTGTTGGTAACGTTAAAATGGCCGCAACTGCAACTGTCTATGCAAACATT
GATGCTTTAATGTCAATCACCACNTCAACCCAAACNTCAATNTCTCAATCATCAACCAAG
ACTGTCAACGGTAAGACCACCACTCAATCATCATCAAGCTCATCATCATCCATCTCTAAA
CAATTCGTTCCAATGATCGTTTGTTCAGGTACTGCTGATGTTGCTGGTAACCTCAATGTC
GTTGCCTCAGAAGCCAATCAAGCTAAAATTGCCTCTGGTACTGTATTCAAATTCATCTCA
GCCCCAGTTGTTAAAGGTAATGTCTCCTCAGTTAGCTGTCTCACCTCAAGCACTGCTGCT
GCTGCTCAACAAGCTGCCGGTGCTGCTGCTGCTGCTGCCCAAAAAGCTGCTATGGCTGCT
GCNTNTAAAGTTTGGTCAATTGTAAATTCACCATGTAACTGTGAATGTGGATTGAAGAGA
TTATAAATTGATTCAACTCCTCCTCTCCAATGACAATAAATTAAAATAATACAACTCATT
CTTATTTTTCTTTATTCTAACCAATGTATTTGTCAATTAACTTATTAACTTTTTAAACAC
ACCCTTTTCAATTTTAAAACAATAAAAATTTACATA
sequence update 2002.10.25
Translated Amino Acid sequence 
fnqtnkkqqfkktiiyskvktikeslivffsssl*ipifffklissfsvflhnhnqpyii
ttnpiplqsyllnnyyllyyfsvylli*YLLASNKQKK---

---VAGGSIESKVIDFTHGTIQGVGXLKCGTCNFANKIAANXNIVGNVKMAATATVYANI
DALMSITTSTQTSXSQSSTKTVNGKTTTQSSSSSSSSISKQFVPMIVCSGTADVAGNLNV
VASEANQAKIASGTVFKFISAPVVKGNVSSVSCLTSSTAAAAQQAAGAAAAAAQKAAMAA
AXKVWSIVNSPCNCECGLKRL*idstpplq*q*ikiiqliliflysnqcicqlty*lfkh
tlfnfktikiyi
Translated Amino Acid sequence (All Frames) 
Frame A:
iqsnkqktti*knnnl**sknn*ritncffllltinsniff*infqlfcfftqsqptlyh
yyqpytitilfik*llfvvlf*cifvdlifisk*qtkkk---

---wslvvqlnqkllislmvpfkvlvxlnvvlvtlpirslltstllvtlkwpqlqlsmqt
lml*cqspxqpkxqxlnhqprlstvrpplnhhqahhhpslnnsfq*sfvqvllmllvtsm
slpqkpiklklplvlysnssqpqllkvmspqlavspqalllllnklpvlllllpkkllwl
lxxkfgql*ihhvtvnvd*rdykliqlllsndnklk*ynsflfffiltnvfvn*linfln
tpfsilkq*kft

Frame B:
fnqtnkkqqfkktiiyskvktikeslivffsssl*ipifffklissfsvflhnhnqpyii
ttnpiplqsyllnnyyllyyfsvylli*YLLASNKQKK---

---grwwfn*iksy*fhswyhsrcwxs*mwyl*lcq*drc*xqhcw*r*ngrncnclckh
*cfnvnhhxnpnxnxsiinqdcqr*dhhsiiikliiihl*tirsndrlfryc*ccw*pqc
rclrsqss*nclwyciqihlspsc*r*clls*lshlkhccccstscrccccccpkscygc
cx*slvnckftm*l*mwieeiin*fnssspmtin*nntthsyfslf*pmylsinlltf*t
hpfqf*nnknlh

Frame C:
sikqtknnnlkkq*fivk*kqlknh*lffspphykfqyffln*fpaflffytittnpisl
lptlyhynliy*iiiicciilvyic*fniy*qvtnkkk---

---VAGGSIESKVIDFTHGTIQGVGXLKCGTCNFANKIAANXNIVGNVKMAATATVYANI
DALMSITTSTQTSXSQSSTKTVNGKTTTQSSSSSSSSISKQFVPMIVCSGTADVAGNLNV
VASEANQAKIASGTVFKFISAPVVKGNVSSVSCLTSSTAAAAQQAAGAAAAAAQKAAMAA
AXKVWSIVNSPCNCECGLKRL*idstpplq*q*ikiiqliliflysnqcicqlty*lfkh
tlfnfktikiyi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLE342 (SLE342Q) /CSM/SL/SLE3-B/SLE342Q.Seq.d/                        900   0.0
SLA101 (SLA101Q) /CSM/SL/SLA1-A/SLA101Q.Seq.d/                        900   0.0
SHL563 (SHL563Q) /CSM/SH/SHL5-C/SHL563Q.Seq.d/                        900   0.0
SHK762 (SHK762Q) /CSM/SH/SHK7-C/SHK762Q.Seq.d/                        900   0.0
SHG713 (SHG713Q) /CSM/SH/SHG7-A/SHG713Q.Seq.d/                        900   0.0
SHG514 (SHG514Q) /CSM/SH/SHG5-A/SHG514Q.Seq.d/                        900   0.0
SHG338 (SHG338Q) /CSM/SH/SHG3-B/SHG338Q.Seq.d/                        900   0.0
SHG152 (SHG152Q) /CSM/SH/SHG1-C/SHG152Q.Seq.d/                        900   0.0
SHF737 (SHF737Q) /CSM/SH/SHF7-B/SHF737Q.Seq.d/                        900   0.0
SHF649 (SHF649Q) /CSM/SH/SHF6-C/SHF649Q.Seq.d/                        900   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U83087|U83087.1 Dictyostelium discoideum rsc12 mRNA, partial cds.   900   0.0    2
AX344185|AX344185.1 Sequence 32 from Patent WO0200926.             40   0.083  4
AX251781|AX251781.1 Sequence 42 from Patent WO0168911.             40   0.083  4
BD460245|BD460245.1 Diagnosis of Diseases Associated with Cell Cycle.    40   0.083  4
BD452167|BD452167.1 Diagnosis of Diseases Associated with Cell Cycle.    40   0.083  4
AJ297856|AJ297856.1 Lucilia sericata partial bcd gene for bicoid protein.    50   0.10   1
AL845362|AL845362.4 Zebrafish DNA sequence from clone CH211-142H2 in linkage group 20.    38   0.33   3
AC156169|AC156169.2 Bos taurus clone CH240-50N13, *** SEQUENCING IN PROGRESS ***, 60 unordered pieces.    48   0.41   1
AC148223|AC148223.1 Colobus guereza clone CH272-19E23, WORKING DRAFT SEQUENCE, 4 ordered pieces.    48   0.41   1
AC127176|AC127176.3 Rattus norvegicus clone CH230-267P11, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    48   0.41   1
dna update 2006. 3.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U83087_1(U83087|pid:none) Dictyostelium discoideum rsc12 mRNA, p...   240   5e-62
AY530539_1(AY530539|pid:none) Mimulus ringens deficiens (DEF) mR...    34   9.2
protein update 2008.11.16
PSORT 

 psg: 0.78  gvh: 0.38  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 32.0 %: cytoplasmic
	 24.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for SHF410 is nuc
5' end seq. ID SHF410F
5' end seq. 
>SHF410F.Seq
ATTCAATCAAACAAACAAAAAACAACAATTTAAAAAAACAATAATTTATAGTAAAGTAAA
AACAATTAAAGAATCACTAATTGTTTTTTTCTCCTCCTCACTATAAATTCCAATATTTTT
TTTTAAATTAATTTCCAGCTTTTCTGTTTTTTTACACAATCACAACCAACCCTATATCAT
TACTACCAACCCTATACCATTACAATCTTATTTATTAAATAATTATTATTTGTTGTATTA
TTTTAGTGTATATTTGTTGATTTAATATTTATTAGCAAGTAACAAACAAAAAAAAAANNN
NNNNNNN
Length of 5' end seq. 307
3' end seq. ID SHF410Z
3' end seq. 
>SHF410Z.Seq
NNNNNNNNNNTGGTCGCTGGTGGTTCAATTGAATCAAAAGTTATTGATTTCACTCATGGT
ACCATTCAAGGTGTTGGTGNTCTTAAATGTGGTACTTGTAACTTTGCCAATAAGATCGCT
GCTAACNTCAACATTGTTGGTAACGTTAAAATGGCCGCAACTGCAACTGTCTATGCAAAC
ATTGATGCTTTAATGTCAATCACCACNTCAACCCAAACNTCAATNTCTCAATCATCAACC
AAGACTGTCAACGGTAAGACCACCACTCAATCATCATCAAGCTCATCATCATCCATCTCT
AAACAATTCGTTCCAATGATCGTTTGTTCAGGTACTGCTGATGTTGCTGGTAACCTCAAT
GTCGTTGCCTCAGAAGCCAATCAAGCTAAAATTGCCTCTGGTACTGTATTCAAATTCATC
TCAGCCCCAGTTGTTAAAGGTAATGTCTCCTCAGTTAGCTGTCTCACCTCAAGCACTGCT
GCTGCTGCTCAACAAGCTGCCGGTGCTGCTGCTGCTGCTGCCCAAAAAGCTGCTATGGCT
GCTGCNTNTAAAGTTTGGTCAATTGTAAATTCACCATGTAACTGTGAATGTGGATTGAAG
AGATTATAAATTGATTCAACTCCTCCTCTCCAATGACAATAAATTAAAATAATACAACTC
ATTCTTATTTTTCTTTATTCTAACCAATGTATTTGTCAATTAACTTATTAACTTTTTAAA
CACACCCTTTTCAATTTTAAAACAATAAAAATTTACATA
Length of 3' end seq. 759
Connected seq. ID SHF410P
Connected seq. 
>SHF410P.Seq
ATTCAATCAAACAAACAAAAAACAACAATTTAAAAAAACAATAATTTATAGTAAAGTAAA
AACAATTAAAGAATCACTAATTGTTTTTTTCTCCTCCTCACTATAAATTCCAATATTTTT
TTTTAAATTAATTTCCAGCTTTTCTGTTTTTTTACACAATCACAACCAACCCTATATCAT
TACTACCAACCCTATACCATTACAATCTTATTTATTAAATAATTATTATTTGTTGTATTA
TTTTAGTGTATATTTGTTGATTTAATATTTATTAGCAAGTAACAAACAAAAAAAAAA---
-------TGGTCGCTGGTGGTTCAATTGAATCAAAAGTTATTGATTTCACTCATGGTACC
ATTCAAGGTGTTGGTGNTCTTAAATGTGGTACTTGTAACTTTGCCAATAAGATCGCTGCT
AACNTCAACATTGTTGGTAACGTTAAAATGGCCGCAACTGCAACTGTCTATGCAAACATT
GATGCTTTAATGTCAATCACCACNTCAACCCAAACNTCAATNTCTCAATCATCAACCAAG
ACTGTCAACGGTAAGACCACCACTCAATCATCATCAAGCTCATCATCATCCATCTCTAAA
CAATTCGTTCCAATGATCGTTTGTTCAGGTACTGCTGATGTTGCTGGTAACCTCAATGTC
GTTGCCTCAGAAGCCAATCAAGCTAAAATTGCCTCTGGTACTGTATTCAAATTCATCTCA
GCCCCAGTTGTTAAAGGTAATGTCTCCTCAGTTAGCTGTCTCACCTCAAGCACTGCTGCT
GCTGCTCAACAAGCTGCCGGTGCTGCTGCTGCTGCTGCCCAAAAAGCTGCTATGGCTGCT
GCNTNTAAAGTTTGGTCAATTGTAAATTCACCATGTAACTGTGAATGTGGATTGAAGAGA
TTATAAATTGATTCAACTCCTCCTCTCCAATGACAATAAATTAAAATAATACAACTCATT
CTTATTTTTCTTTATTCTAACCAATGTATTTGTCAATTAACTTATTAACTTTTTAAACAC
ACCCTTTTCAATTTTAAAACAATAAAAATTTACATA
Length of connected seq. 1046
Full length Seq ID -
Full length Seq. -
Length of full length seq. -