>AFJ339 (AFJ339Q) /CSM/AF/AFJ3-B/AFJ339Q.Seq.d/
XXXXXXXXXXTATTCATTGACTCCACTCAAGATAACACTCAAGTTCTCGAAGATGTAAAG
AAAATCGCTACCTCACAAAAAGGTAATGCCGTTTTCTGTTGGGTCGATATGAAGAAATTC
CCACAACAAGCAACTCATATGGGTCTCTCTGGTAAAGTTGTTCCAGCCATCTCTGTTGAT
TCAGTTGCCAATAAAGCTCGTTACAATTTCGATGAAAAAGAAACCTTCTCATTCGATACC
GTCTCCAAATGGATCCAAGATGTCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCAA
CCAGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAAAAG
TTGGTTTTAGACTCACCAAAGGATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTCAT
TGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAATACTTAAAAGACGTTGAATCT
GTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTACCAAGTGATATCGAAATTAGA
GGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAAGAAAATCCAATCTCTTATGAA
GGTCAAAGAAANGATCATATGAACTTTGTTGAATTCATTCAAGATAACGCTGCCATNAAT
TCAAATTACCATCATCTCAAACTGAGATAATGTNAATCTAAAAAAGATTCTTCTGCTAA

---FIDSTQDNTQVLEDVKKIATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISVDS
VANKARYNFDEKETFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKKL
VLDSPKDVLVEFYAPWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIRG
YPTIMLFKADDKENPISYEGQRXDHMNFVEFIQDNAAXNSNYHHLKLR*cxskkdssa

Frame A:
---ih*lhsr*hsssrrckenryltkr*crfllgryeeipttsnsygslw*scsshlc*f
scq*sslqfr*krnlliryrlqmdprchww*sfsic*istrtrik*css*gcrwyhfqkv
gfrltkgcfg*ilrsmvrslqescsnlr*ir*ilkrr*iclnrkd*crlk*ctk*yrn*r
lpnhhalqs**qrksnll*rskkxsyelc*ihsr*rchxfklpssqteimxi*krffc*


Frame B:
---FIDSTQDNTQVLEDVKKIATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISVDS
VANKARYNFDEKETFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKKL
VLDSPKDVLVEFYAPWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIRG
YPTIMLFKADDKENPISYEGQRXDHMNFVEFIQDNAAXNSNYHHLKLR*cxskkdssa


Frame C:
---sltplkitlkfskm*rkslphkkvmpfsvgsi*rnshnkqliwvslvklfqpslliq
lpiklvtismkkkpshsipspngskmslvvkflhllnlnqnqnqmmlqlrlplvplsksw
f*thqrmfwlnstlhgaviarillqstin*vnt*ktlnlsqs*rlmptqmmyqvisklev
tqpscssklmtkkiqslmkvkexii*tllnsfkitlpxiqitiisn*dnvnlkkilll

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFJ339 (AFJ339Q) /CSM/AF/AFJ3-B/AFJ339Q.Seq.d/                       1388   0.0
AFK319 (AFK319Q) /CSM/AF/AFK3-A/AFK319Q.Seq.d/                       1374   0.0
AFK269 (AFK269Q) /CSM/AF/AFK2-C/AFK269Q.Seq.d/                       1374   0.0
AFK217 (AFK217Q) /CSM/AF/AFK2-A/AFK217Q.Seq.d/                       1374   0.0
AFJ720 (AFJ720Q) /CSM/AF/AFJ7-A/AFJ720Q.Seq.d/                       1368   0.0
AFJ889 (AFJ889Q) /CSM/AF/AFJ8-D/AFJ889Q.Seq.d/                       1362   0.0
AFO368 (AFO368Q) /CSM/AF/AFO3-C/AFO368Q.Seq.d/                       1360   0.0
AFN894 (AFN894Q) /CSM/AF/AFN8-D/AFN894Q.Seq.d/                       1360   0.0
AFN855 (AFN855Q) /CSM/AF/AFN8-C/AFN855Q.Seq.d/                       1360   0.0
AFM464 (AFM464Q) /CSM/AF/AFM4-C/AFM464Q.Seq.d/                       1360   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end,single read.   593   e-165  1
CN206669|CN206669.1 Tor7097 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    78   2e-10  1
CN591465|CN591465.1 TTE00010235 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN597971|CN597971.1 TTE00009997 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN590755|CN590755.1 TTE00006978 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CB176061|CB176061.1 pl17e07.y3 Ancylostoma ceylanicum adult Ancylostoma ceylanicum cDNA 5' similar to TR:O17908 O17908 H06O01.1 PROTEIN. [1] ;, mRNA sequence.    70   5e-08  1
CN206834|CN206834.1 Tor7258 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    70   5e-08  1
CK030146|CK030146.1 AGENCOURT_16621384 NIH_ZGC_7 Danio rerio cDNA clone IMAGE:7052631 5', mRNA sequence.    70   5e-08  1
AL924030|AL924030.1 Danio rerio EST sequence, clone 134-B07-2.     70   5e-08  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   177   4e-43
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   173   4e-42
EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   170   3e-41
CU633901_146(CU633901|pid:none) Podospora anserina genomic DNA c...   167   3e-40
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   165   1e-39
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   160   3e-38
CR382131_125(CR382131|pid:none) Yarrowia lipolytica strain CLIB1...   152   9e-36
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   151   2e-35
BC046707_1(BC046707|pid:none) Xenopus laevis glucose regulated p...   150   3e-35
(Q10057) RecName: Full=Putative protein disulfide-isomerase C1F5...   150   3e-35

 psg: 0.75  gvh: 0.36  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 28.0 %: cytoplasmic
	 20.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall

>> prediction for AFJ339 is nuc

>AFJ339Z.Seq
----------TATTCATTGACTCCACTCAAGATAACACTCAAGTTCTCGAAGATGTAAAG
AAAATCGCTACCTCACAAAAAGGTAATGCCGTTTTCTGTTGGGTCGATATGAAGAAATTC
CCACAACAAGCAACTCATATGGGTCTCTCTGGTAAAGTTGTTCCAGCCATCTCTGTTGAT
TCAGTTGCCAATAAAGCTCGTTACAATTTCGATGAAAAAGAAACCTTCTCATTCGATACC
GTCTCCAAATGGATCCAAGATGTCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCAA
CCAGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAAAAG
TTGGTTTTAGACTCACCAAAGGATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTCAT
TGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAATACTTAAAAGACGTTGAATCT
GTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTACCAAGTGATATCGAAATTAGA
GGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAAGAAAATCCAATCTCTTATGAA
GGTCAAAGAAANGATCATATGAACTTTGTTGAATTCATTCAAGATAACGCTGCCATNAAT
TCAAATTACCATCATCTCAAACTGAGATAATGTNAATCTAAAAAAGATTCTTCTGCTAA