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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHF346
Library SH
(Link to library)
Clone ID SHF346
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12649-1|Contig-U16394-1
Original site URL
Representative seq. ID SHF346P
(Link to Original site)
Representative DNA sequence 
>SHF346 (SHF346Q) /CSM/SH/SHF3-B/SHF346Q.Seq.d/
GCAATAATTAAAACCAACAATAGCAATAATAATTATAATAATAATAAATAATAATAATAA
TAATAACAATAAAATAGTAACACCAATAAATAATAATATTAATAATAATAATAATAATAA
TAATAATAATAATAAAATAAAAAATATAATAATAATAATAGTAATAATAGTAATAATAAT
AATAAAATAATAATAATAGAAATAATTTCAATTTAAAAAATAAATAAAATAAAAAAAGAA
AAAAATAAAAAAAAAAATAAATTTTTTTTTTAAAAAATTAAAANTTTAAAAAAAAAAXXX
XXXXXXXAAAGTATGACATTGGTTGGGACAGATTCTTTCAATTCACCATGCAAAATACAT
TGGGTGTACAGTGTCTTCACAATTGGACACTTCAAATTGTACACAGAGACTTTAAGAGTT
TAAATCTTTTGGTCAATGAAGATTGGGAATGCAAAGTTTCAGATTTTGGTCTTAGTCGTT
TTAATACCGCCGATAATTTAGAAACACTCAGTAAGATTCGTGGTACNTTTGCNTATTGTT
CACNTGAAGTCGCCGTAGGTAATGGTAGTCTCTACACTACCAAATNTGATATNTACAGTA
TTGGTATCGTCTTTTGGGAATTAGTAACTCGTGTTATCAATGGTGAATACTCTCGTCCNT
ACAGTGAATATTCTCACATCAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
aiiktnnsnnnynnnk******q*nsntnk**y************nkkynnnnsnnsnnn
nkiiiieiisi*kinkikkeknkkknkfff*KIKXLKKK---

---KYDIGWDRFFQFTMQNTLGVQCLHNWTLQIVHRDFKSLNLLVNEDWECKVSDFGLSR
FNTADNLETLSKIRGTFAYCSXEVAVGNGSLYTTKXDXYSIGIVFWELVTRVINGEYSRP
YSEYSHIK
Translated Amino Acid sequence (All Frames) 
Frame A:
aiiktnnsnnnynnnk******q*nsntnk**y************nkkynnnnsnnsnnn
nkiiiieiisi*kinkikkeknkkknkfff*KIKXLKKK---

---kv*hwlgqilsihhakyigctvssqldtsnctqrl*efksfgq*rlgmqsfrfws*s
f*yrr*frntq*dswyxcxlft*srrr*w*slhyqx*yxqywyrllgisnscyqw*ilss
xq*ifshq

Frame B:
q*lkptiaiiiiiiinnnnnnnnkivtpinnninnnnnnnnnnnkikniiiiiviiviii
ik****k*fqfkk*ik*kkkkikkkinfffkklkx*kk---

---KYDIGWDRFFQFTMQNTLGVQCLHNWTLQIVHRDFKSLNLLVNEDWECKVSDFGLSR
FNTADNLETLSKIRGTFAYCSXEVAVGNGSLYTTKXDXYSIGIVFWELVTRVINGEYSRP
YSEYSHIK

Frame C:
nn*nqq*q**l****iiiiiitik**hq*iiiliiiiiiiiiiik*ki************
*nnnnrnnfnlknk*nkkrkk*kkk*ifflkn*xfkkk---

---smtlvgtdsfnspckihwvysvftighfklytetlrv*ifwsmkignakfqilvlvv
lippii*khsvrfvvxlxivhxksp*vmvvstlpnxixtvlvssfgn**lvlsmvntlvx
tvniltsk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHF346 (SHF346Q) /CSM/SH/SHF3-B/SHF346Q.Seq.d/                        712   0.0
SHE857 (SHE857Q) /CSM/SH/SHE8-C/SHE857Q.Seq.d/                        688   0.0
SHB434 (SHB434Q) /CSM/SH/SHB4-B/SHB434Q.Seq.d/                        688   0.0
CHS225 (CHS225Q) /CSM/CH/CHS2-B/CHS225Q.Seq.d/                        688   0.0
SHF208 (SHF208Q) /CSM/SH/SHF2-A/SHF208Q.Seq.d/                        682   0.0
AHF423 (AHF423Q) /CSM/AH/AHF4-A/AHF423Q.Seq.d/                        680   0.0
CHP295 (CHP295Q) /CSM/CH/CHP2-D/CHP295Q.Seq.d/                        672   0.0
SHD120 (SHD120Q) /CSM/SH/SHD1-A/SHD120Q.Seq.d/                        668   0.0
SHK887 (SHK887Q) /CSM/SH/SHK8-D/SHK887Q.Seq.d/                        636   0.0
VHB666 (VHB666Q) /CSM/VH/VHB6-C/VHB666Q.Seq.d/                        630   e-180
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC115604|AC115604.2 Dictyostelium discoideum chromosome 2 map 4354771-4414991 strain AX4, complete sequence.    42   4e-06  2
DW018299|DW018299.1 EST1227260 MTY Medicago truncatula cDNA clone MTYB479, mRNA sequence.    48   0.26   1
BG646682|BG646682.1 EST508301 HOGA Medicago truncatula cDNA clone pHOGA-9D1 5' end, mRNA sequence.    48   0.26   1
BG645865|BG645865.1 EST507484 KV3 Medicago truncatula cDNA clone pKV3-47F12 5' end, mRNA sequence.    48   0.26   1
BG453581|BG453581.1 NF092A07LF1F1050 Developing leaf Medicago truncatula cDNA clone NF092A07LF 5', mRNA sequence.    48   0.26   1
BG453480|BG453480.1 NF092C08LF1F1055 Developing leaf Medicago truncatula cDNA clone NF092C08LF 5', mRNA sequence.    48   0.26   1
BF647893|BF647893.1 NF038B09EC1F1076 Elicited cell culture Medicago truncatula cDNA clone NF038B09EC 5', mRNA sequence.    48   0.26   1
BF646211|BF646211.1 NF067F06EC1F1058 Elicited cell culture Medicago truncatula cDNA clone NF067F06EC 5', mRNA sequence.    48   0.26   1
BF644581|BF644581.1 NF016B08EC1F1064 Elicited cell culture Medicago truncatula cDNA clone NF016B08EC 5', mRNA sequence.    48   0.26   1
AW685406|AW685406.1 NF028H12NR1F1000 Nodulated root Medicago truncatula cDNA clone NF028H12NR 5', mRNA sequence.    48   0.26   1
dna update 2006. 3.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54XX5) RecName: Full=Probable serine/threonine-protein kinase ...   252   6e-66
(Q55GG4) RecName: Full=Probable serine/threonine-protein kinase ...   132   9e-30
(Q54G28) RecName: Full=Probable tyrosine-protein kinase DDB_G029...   125   9e-28
(Q54HC6) RecName: Full=Ankyrin repeat-containing protein kinase ...   124   2e-27
(Q54RR9) RecName: Full=Probable serine/threonine-protein kinase ...   108   2e-22
(Q86HG9) RecName: Full=Probable serine/threonine-protein kinase ...    84   5e-15
AM477141_2(AM477141|pid:none) Vitis vinifera contig VV78X035827....    77   5e-13
AC159538_19(AC159538|pid:none) Medicago truncatula clone mth2-48...    76   8e-13
AL732347_6(AL732347|pid:none) Oryza sativa genomic DNA, chromoso...    76   1e-12
AY224496_1(AY224496|pid:none) Oryza sativa (japonica cultivar-gr...    76   1e-12
protein update 2009. 4.18
PSORT 

 psg: 0.63  gvh: 0.43  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	 16.0 %: mitochondrial
	  8.0 %: vesicles of secretory system

>> prediction for SHF346 is cyt
5' end seq. ID SHF346F
5' end seq. 
>SHF346F.Seq
GCAATAATTAAAACCAACAATAGCAATAATAATTATAATAATAATAAATAATAATAATAA
TAATAACAATAAAATAGTAACACCAATAAATAATAATATTAATAATAATAATAATAATAA
TAATAATAATAATAAAATAAAAAATATAATAATAATAATAGTAATAATAGTAATAATAAT
AATAAAATAATAATAATAGAAATAATTTCAATTTAAAAAATAAATAAAATAAAAAAAGAA
AAAAATAAAAAAAAAAATAAATTTTTTTTTTAAAAAATTAAAANTTTAAAAAAAAAANNN
NNNNNNN
Length of 5' end seq. 307
3' end seq. ID SHF346Z
3' end seq. 
>SHF346Z.Seq
NNNNNNNNNNAAAGTATGACATTGGTTGGGACAGATTCTTTCAATTCACCATGCAAAATA
CATTGGGTGTACAGTGTCTTCACAATTGGACACTTCAAATTGTACACAGAGACTTTAAGA
GTTTAAATCTTTTGGTCAATGAAGATTGGGAATGCAAAGTTTCAGATTTTGGTCTTAGTC
GTTTTAATACCGCCGATAATTTAGAAACACTCAGTAAGATTCGTGGTACNTTTGCNTATT
GTTCACNTGAAGTCGCCGTAGGTAATGGTAGTCTCTACACTACCAAATNTGATATNTACA
GTATTGGTATCGTCTTTTGGGAATTAGTAACTCGTGTTATCAATGGTGAATACTCTCGTC
CNTACAGTGAATATTCTCACATCAAAA
Length of 3' end seq. 387
Connected seq. ID SHF346P
Connected seq. 
>SHF346P.Seq
GCAATAATTAAAACCAACAATAGCAATAATAATTATAATAATAATAAATAATAATAATAA
TAATAACAATAAAATAGTAACACCAATAAATAATAATATTAATAATAATAATAATAATAA
TAATAATAATAATAAAATAAAAAATATAATAATAATAATAGTAATAATAGTAATAATAAT
AATAAAATAATAATAATAGAAATAATTTCAATTTAAAAAATAAATAAAATAAAAAAAGAA
AAAAATAAAAAAAAAAATAAATTTTTTTTTTAAAAAATTAAAANTTTAAAAAAAAAA---
-------AAAGTATGACATTGGTTGGGACAGATTCTTTCAATTCACCATGCAAAATACAT
TGGGTGTACAGTGTCTTCACAATTGGACACTTCAAATTGTACACAGAGACTTTAAGAGTT
TAAATCTTTTGGTCAATGAAGATTGGGAATGCAAAGTTTCAGATTTTGGTCTTAGTCGTT
TTAATACCGCCGATAATTTAGAAACACTCAGTAAGATTCGTGGTACNTTTGCNTATTGTT
CACNTGAAGTCGCCGTAGGTAATGGTAGTCTCTACACTACCAAATNTGATATNTACAGTA
TTGGTATCGTCTTTTGGGAATTAGTAACTCGTGTTATCAATGGTGAATACTCTCGTCCNT
ACAGTGAATATTCTCACATCAAAA
Length of connected seq. 674
Full length Seq ID -
Full length Seq. -
Length of full length seq. -