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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHF327
Library SH
(Link to library)
Clone ID SHF327
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U11244-1
Original site URL
Representative seq. ID SHF327P
(Link to Original site)
Representative DNA sequence 
>SHF327 (SHF327Q) /CSM/SH/SHF3-B/SHF327Q.Seq.d/
ATTATTTTTGTACTTTTATTATTTTATTTTAATTATTATTATAATTTATCAACAACGCAT
TTTGATTATTAATATTTTTTTAATCATTAGTAAAAACACAAATAAAATTTAGTCAACATA
CAAAAAAAAAAXXXXXXXXXXTATTGAAATTAAGATTGCATTTGATCAATTTCAAGAAGC
AGAAAAGAATTTCCAAGAGTTGGCAGTGTTATTTGGTGAAGAATNTAAAATACCCTNTGA
GGAATTCTTTGTTACAATCAATCGTTTCATCGTTATGTTTGATAAATGTTATAAAGATTT
CCAAAGAGATAAAGAAGCTGCTGAACGTGCAATTAAAAGGGATGAAGCAAAAGCAAAGAA
AGCTCAACAATTAAAGAGAATGAATGGTAAAATAGCCTCAAGTACAAATAATAAAAATCC
ATTGGCTTCAAGTTCAACTTCTGTTGGTGATGGTGGTATGGTTGAAGATATCATGCAATC
AGTTAGAGATGGTGATGCTTTTAAACAAAGAAGACGTCTAAAAGGTACAAAAGAAAATAC
TGATGATTGCTAGTTCCATCACCACTATCTNTGAACAATNTGAAAATTNTAATNCCTCTT
CAATCACCATCACTACTCCAAGTGGTATTGAATTGGATATTACCC
sequence update 2002.10.25
Translated Amino Acid sequence 
yfctfiilf*lll*finnaf*lliff*SLVKTQIKFSQHTKKK---

---IEIKIAFDQFQEAEKNFQELAVLFGEEXKIPXEEFFVTINRFIVMFDKCYKDFQRDK
EAAERAIKRDEAKAKKAQQLKRMNGKIASSTNNKNPLASSSTSVGDGGMVEDIMQSVRDG
DAFKQRRRLKGTKENTDDC*fhhhyl*tx*kx*xlfnhhhyskwy*igyy
Translated Amino Acid sequence (All Frames) 
Frame A:
iifvlllfyfnyyynlstthfdy*yffnh**khk*nlvniqkk---

---y*n*dci*sisrsrkefprvgsviw*rx*ntl*gilcynqsfhryv**ml*rfpkr*
rsc*tcn*kg*skskesstikenew*nslkyk**ksigfkfnfcw*wwyg*ryhais*rw
*cf*tkktskrykrky**llvpsplsxnnxkixxplqspsllqvvlnwilp

Frame B:
lflyfyyfiliiiiiyqqriliinifliiskntnki*stykkk---

---IEIKIAFDQFQEAEKNFQELAVLFGEEXKIPXEEFFVTINRFIVMFDKCYKDFQRDK
EAAERAIKRDEAKAKKAQQLKRMNGKIASSTNNKNPLASSSTSVGDGGMVEDIMQSVRDG
DAFKQRRRLKGTKENTDDC*fhhhyl*tx*kx*xlfnhhhyskwy*igyy

Frame C:
yfctfiilf*lll*finnaf*lliff*SLVKTQIKFSQHTKKK---

---lklrlhlinfkkqkriskswqcylvknxkypxrnsllqsivsslclinvikiskeik
kllnvqlkgmkqkqrklnn*re*mvk*pqvqiikihwlqvqlllvmvvwlkiscnqlemv
mllnkedv*kvqkkilmiassittixeqxenxnxssitittpsgieldit
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHF327 (SHF327Q) /CSM/SH/SHF3-B/SHF327Q.Seq.d/                        963   0.0
SHF525 (SHF525Q) /CSM/SH/SHF5-B/SHF525Q.Seq.d/                        948   0.0
SFA107 (SFA107Q) /CSM/SF/SFA1-A/SFA107Q.Seq.d/                        948   0.0
CHF806 (CHF806Q) /CSM/CH/CHF8-A/CHF806Q.Seq.d/                        948   0.0
CHF689 (CHF689Q) /CSM/CH/CHF6-D/CHF689Q.Seq.d/                        948   0.0
AHK164 (AHK164Q) /CSM/AH/AHK1-C/AHK164Q.Seq.d/                        948   0.0
AHF519 (AHF519Q) /CSM/AH/AHF5-A/AHF519Q.Seq.d/                        948   0.0
AHB713 (AHB713Q) /CSM/AH/AHB7-A/AHB713Q.Seq.d/                        948   0.0
VHH885 (VHH885Q) /CSM/VH/VHH8-D/VHH885Q.Seq.d/                        924   0.0
AHD419 (AHD419Q) /CSM/AH/AHD4-A/AHD419Q.Seq.d/                        870   0.0
own update 2002.12. 5
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AJ812237|AJ812237.1 Dictyostelium discoideum mRNA for diaphanous-related formin dDia1  (drf1 gene).   795   0.0    2
AX320410|AX320410.1 Sequence 33 from Patent WO0168858.             50   0.062  1
AL589674|AL589674.9 Human DNA sequence from clone RP11-137J7 on chromosome 6.    50   0.062  1
AA273165|AA273165.1 SW3ICA2592SK Brugia malayi infective larva cDNA (SAW94WL-BmL3) Brugia malayi cDNA clone SW3ICA2592 5', mRNA sequence.    48   0.25   1
CK850724|CK850724.1 SWWbL3CAW25C09SK Wuchereria bancrofti L3 cDNA (SAW96MLW-WbL3) Wuchereria bancrofti cDNA clone SWWbL3CAW25C09 5', mRNA sequence.    48   0.25   1
AI065990|AI065990.1 BSBmMFSZ04O21SK Brugia malayi microfilaria cDNA (SAW94LS-BmMf) Brugia malayi cDNA clone BSBmMFSZ04O21 5', mRNA sequence.    48   0.25   1
DU872868|DU872868.1 105956 Tomato HindIII BAC Library Lycopersicon esculentum genomic clone LE_HBa0182H11 3, genomic survey sequence.    48   0.25   1
DU849041|DU849041.1 43526 Tomato HindIII BAC Library Lycopersicon esculentum genomic clone LE_HBa0053C20 3, genomic survey sequence.    48   0.25   1
AY330618|AY330618.1 Brugia malayi independent phosphoglycerate mutase isoform 2 (iPGM) mRNA, complete cds.    48   0.25   1
AY330617|AY330617.1 Brugia malayi independent phosphoglycerate mutase isoform 1 (iPGM) mRNA, complete cds.    48   0.25   1
dna update 2006. 3.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54WH2) RecName: Full=Formin-A;                                       62   1e-08
AB082542_1(AB082542|pid:none) Dictyostelium discoideum forA mRNA...    62   1e-08
(Q5TJ57) RecName: Full=Formin-E; AltName: Full=Diaphanous-relate...    60   7e-08
AL031640_2(AL031640|pid:none) Drosophila melanogaster cosmid clo...    48   2e-04
AE014298_186(AE014298|pid:none) Drosophila melanogaster chromoso...    48   2e-04
AE014298_187(AE014298|pid:none) Drosophila melanogaster chromoso...    48   2e-04
AY118439_1(AY118439|pid:none) Drosophila melanogaster RH61354 fu...    47   6e-04
AK088648_1(AK088648|pid:none) Mus musculus 2 days neonate thymus...    42   0.014
(O70566) RecName: Full=Protein diaphanous homolog 2; AltName: Fu...    42   0.019
AL683856_2(AL683856|pid:none) Mouse DNA sequence from clone RP23...    42   0.019
protein update 2009. 4.18
PSORT 

 psg: 0.46  gvh: 0.35  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 24.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  8.0 %: vesicles of secretory system
	  4.0 %: cytoskeletal

>> prediction for SHF327 is nuc
5' end seq. ID SHF327F
5' end seq. 
>SHF327F.Seq
ATTATTTTTGTACTTTTATTATTTTATTTTAATTATTATTATAATTTATCAACAACGCAT
TTTGATTATTAATATTTTTTTAATCATTAGTAAAAACACAAATAAAATTTAGTCAACATA
CAAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 141
3' end seq. ID SHF327Z
3' end seq. 
>SHF327Z.Seq
NNNNNNNNNNTATTGAAATTAAGATTGCATTTGATCAATTTCAAGAAGCAGAAAAGAATT
TCCAAGAGTTGGCAGTGTTATTTGGTGAAGAATNTAAAATACCCTNTGAGGAATTCTTTG
TTACAATCAATCGTTTCATCGTTATGTTTGATAAATGTTATAAAGATTTCCAAAGAGATA
AAGAAGCTGCTGAACGTGCAATTAAAAGGGATGAAGCAAAAGCAAAGAAAGCTCAACAAT
TAAAGAGAATGAATGGTAAAATAGCCTCAAGTACAAATAATAAAAATCCATTGGCTTCAA
GTTCAACTTCTGTTGGTGATGGTGGTATGGTTGAAGATATCATGCAATCAGTTAGAGATG
GTGATGCTTTTAAACAAAGAAGACGTCTAAAAGGTACAAAAGAAAATACTGATGATTGCT
AGTTCCATCACCACTATCTNTGAACAATNTGAAAATTNTAATNCCTCTTCAATCACCATC
ACTACTCCAAGTGGTATTGAATTGGATATTACCC
Length of 3' end seq. 514
Connected seq. ID SHF327P
Connected seq. 
>SHF327P.Seq
ATTATTTTTGTACTTTTATTATTTTATTTTAATTATTATTATAATTTATCAACAACGCAT
TTTGATTATTAATATTTTTTTAATCATTAGTAAAAACACAAATAAAATTTAGTCAACATA
CAAAAAAAAAA----------TATTGAAATTAAGATTGCATTTGATCAATTTCAAGAAGC
AGAAAAGAATTTCCAAGAGTTGGCAGTGTTATTTGGTGAAGAATNTAAAATACCCTNTGA
GGAATTCTTTGTTACAATCAATCGTTTCATCGTTATGTTTGATAAATGTTATAAAGATTT
CCAAAGAGATAAAGAAGCTGCTGAACGTGCAATTAAAAGGGATGAAGCAAAAGCAAAGAA
AGCTCAACAATTAAAGAGAATGAATGGTAAAATAGCCTCAAGTACAAATAATAAAAATCC
ATTGGCTTCAAGTTCAACTTCTGTTGGTGATGGTGGTATGGTTGAAGATATCATGCAATC
AGTTAGAGATGGTGATGCTTTTAAACAAAGAAGACGTCTAAAAGGTACAAAAGAAAATAC
TGATGATTGCTAGTTCCATCACCACTATCTNTGAACAATNTGAAAATTNTAATNCCTCTT
CAATCACCATCACTACTCCAAGTGGTATTGAATTGGATATTACCC
Length of connected seq. 635
Full length Seq ID -
Full length Seq. -
Length of full length seq. -