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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHF243
Library SH
(Link to library)
Clone ID SHF243
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13257-1|Contig-U15745-1
Original site URL
Representative seq. ID SHF243P
(Link to Original site)
Representative DNA sequence 
>SHF243 (SHF243Q) /CSM/SH/SHF2-B/SHF243Q.Seq.d/
CATAAAAAACAAATTGAATTAACTCATGCTTGTACAGTTGGTGATGTAAATTATTTAGAT
CAAATTATTTTATCTGGTGTTTCAACAGATCAATTAAAAGAAGCACATAAAGCAGGACAT
GCAATTGTATTTGATTCATTATTAACACATAATAGTAATTCATTAGTTGTTACAGGTACA
ATGGCATTATTAGGAGCGATTGTAGAGAATGGAGATAGAAAAGGTAAACGTTTATCATTA
TCAAGATCGAATTTATCTAGATTCCCAATGTCGATAACACAAATGTGTACTCATTTGGTT
GAGTTAGATTTAAGTGATAATAAAATCACCGAATTACCAAAGGATATCCAATTGTTAAAG
TCTTTGAGAATTTTAATTTTACGTGGTAATCTATTGGAAGATATACCATTGGAGATTTGT
TATTTAGGAGATTTAAAGATTTTAGAGTTACAAGAAAATCCATTGAATAATTTCCCATTG
AGTGTTGTTCAAAGTGGTACAAAGAATCTTTTATTATTTTGTAAGAATATATTGGAGCGT
AAAAAGAGTGAAACTTGGAATAAGGTCAAGTTGATGTTTGTTGGXXXXXXXXXXGGAAGG
TAAAGNATGAATTCNAGTTACAAAAGGTTCCNANTTTAGNCNCNCATCAGGAAAAGATTA
CCTNTGTTATCCANNTAGAGGATAATGTNTTATCGGG
sequence update 2002.10.25
Translated Amino Acid sequence 
HKKQIELTHACTVGDVNYLDQIILSGVSTDQLKEAHKAGHAIVFDSLLTHNSNSLVVTGT
MALLGAIVENGDRKGKRLSLSRSNLSRFPMSITQMCTHLVELDLSDNKITELPKDIQLLK
SLRILILRGNLLEDIPLEICYLGDLKILELQENPLNNFPLSVVQSGTKNLLLFCKNILER
KKSETWNKVKLMFV---

---KVKXEFXLQKVPXLXXHQEKITXVIXXEDNVLS
Translated Amino Acid sequence (All Frames) 
Frame A:
HKKQIELTHACTVGDVNYLDQIILSGVSTDQLKEAHKAGHAIVFDSLLTHNSNSLVVTGT
MALLGAIVENGDRKGKRLSLSRSNLSRFPMSITQMCTHLVELDLSDNKITELPKDIQLLK
SLRILILRGNLLEDIPLEICYLGDLKILELQENPLNNFPLSVVQSGTKNLLLFCKNILER
KKSETWNKVKLMFV---

---gr*xmnssykrfxx*xxirkrlpxlsx*rimxyr

Frame B:
iknkln*lmlvqlvm*ii*iklfylvfqqin*kkhikqdmqlylihy*hiivih*llqvq
why*erl*rmeiekvnvyhyqdriyldsqcr*hkcvliwls*i*viikspnyqrisnc*s
l*ef*fyvviywkiyhwrfvi*ei*rf*sykkih*iish*vlfkvvqrifyyfvriywsv
krvklgirss*cll---

---egkx*ixvtkgsxfxxxsgkdylcypxrg*cxig

Frame C:
*ktn*insclysw*cklfrsnyfiwcfnrsikrst*srtcnci*fiint***fiscyryn
giirsdcrewr*kr*tfiiikiefi*ipnvdntnvysfg*vrfk***nhritkgypivkv
fenfnftw*sigrytigdllfrrfkdfrvtrksie*fpieccskwykesfiil*eyiga*
ke*nle*gqvdvcw---

---KVKXEFXLQKVPXLXXHQEKITXVIXXEDNVLS
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHF243 (SHF243Q) /CSM/SH/SHF2-B/SHF243Q.Seq.d/                       1257   0.0
CHQ893 (CHQ893Q) /CSM/CH/CHQ8-D/CHQ893Q.Seq.d/                        109   6e-23
VHA395 (VHA395Q) /CSM/VH/VHA3-D/VHA395Q.Seq.d/                        107   2e-22
SHC329 (SHC329Q) /CSM/SH/SHC3-B/SHC329Q.Seq.d/                        107   2e-22
CHR332 (CHR332Q) /CSM/CH/CHR3-B/CHR332Q.Seq.d/                        107   2e-22
CHP206 (CHP206Q) /CSM/CH/CHP2-A/CHP206Q.Seq.d/                        107   2e-22
CHO633 (CHO633Q) /CSM/CH/CHO6-B/CHO633Q.Seq.d/                        107   2e-22
CHN363 (CHN363Q) /CSM/CH/CHN3-C/CHN363Q.Seq.d/                        107   2e-22
CHM424 (CHM424Q) /CSM/CH/CHM4-A/CHM424Q.Seq.d/                        107   2e-22
CHL635 (CHL635Q) /CSM/CH/CHL6-B/CHL635Q.Seq.d/                        107   2e-22
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AY232266|AY232266.1 Dictyostelium discoideum putative protein Roco4 (roco4) gene, complete cds.   985   0.0    3
AY232265|AY232265.1 Dictyostelium discoideum putative protein Roco2 (roco2) gene, complete cds.    64   3e-24  6
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.    64   1e-18  6
AY232273|AY232273.1 Dictyostelium discoideum putative protein Roco11 (roco11) gene, complete cds.    62   2e-09  3
DT250713|DT250713.1 JGI_CAAU4411.rev CAAU Pimephales promelas brain 7-8 month adults, males and females pooled (L) Pimephales promelas cDNA clone CAAU4411 3', mRNA sequence.    50   0.067  1
AP008217|AP008217.1 Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 11.    44   0.22   2
BE401607|BE401607.1 CNW03PL506 ITEC CNW Wheat Powdery Mildew Resistant Library Triticum aestivum cDNA clone CNW03PL506, mRNA sequence.    42   0.24   2
CC810021|CC810021.1 ZMMBBc0497L19f ZMMBBc Zea mays subsp. mays genomic clone ZMMBBc0497L19 5', genomic survey sequence.    48   0.27   1
CT574590|CT574590.3 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    34   0.56   2
BX088595|BX088595.7 Zebrafish DNA sequence from clone DKEY-218B7 in linkage group 6.    34   0.56   2
dna update 2006. 3.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q6XHB2) RecName: Full=Probable serine/threonine-protein kinase ...   361   1e-98
(Q8SSS9) RecName: Full=Probable serine/threonine-protein kinase ...   213   3e-54
(Q6XHA5) RecName: Full=Probable serine/threonine-protein kinase ...   202   1e-50
CP000099_2371(CP000099|pid:none) Methanosarcina barkeri str. Fus...    61   4e-08
(A2Q9L0) RecName: Full=Glucose-repressible alcohol dehydrogenase...    59   1e-07
AE010300_873(AE010300|pid:none) Leptospira interrogans serovar l...    59   1e-07
(Q5B778) RecName: Full=Glucose-repressible alcohol dehydrogenase...    58   2e-07
CP001344_1836(CP001344|pid:none) Cyanothece sp. PCC 7425, comple...    58   3e-07
AM920437_269(AM920437|pid:none) Penicillium chrysogenum Wisconsi...    57   4e-07
CP000393_3313(CP000393|pid:none) Trichodesmium erythraeum IMS101...    57   5e-07
protein update 2009. 4.18
PSORT 

 psg: 0.85  gvh: 0.59  alm: 0.36  top: 0.27  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 24.0 %: nuclear
	 16.0 %: extracellular, including cell wall
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for SHF243 is cyt
5' end seq. ID SHF243F
5' end seq. 
>SHF243F.Seq
CATAAAAAACAAATTGAATTAACTCATGCTTGTACAGTTGGTGATGTAAATTATTTAGAT
CAAATTATTTTATCTGGTGTTTCAACAGATCAATTAAAAGAAGCACATAAAGCAGGACAT
GCAATTGTATTTGATTCATTATTAACACATAATAGTAATTCATTAGTTGTTACAGGTACA
ATGGCATTATTAGGAGCGATTGTAGAGAATGGAGATAGAAAAGGTAAACGTTTATCATTA
TCAAGATCGAATTTATCTAGATTCCCAATGTCGATAACACAAATGTGTACTCATTTGGTT
GAGTTAGATTTAAGTGATAATAAAATCACCGAATTACCAAAGGATATCCAATTGTTAAAG
TCTTTGAGAATTTTAATTTTACGTGGTAATCTATTGGAAGATATACCATTGGAGATTTGT
TATTTAGGAGATTTAAAGATTTTAGAGTTACAAGAAAATCCATTGAATAATTTCCCATTG
AGTGTTGTTCAAAGTGGTACAAAGAATCTTTTATTATTTTGTAAGAATATATTGGAGCGT
AAAAAGAGTGAAACTTGGAATAAGGTCAAGTTGATGTTTGTTGGNNNNNNNNNN
Length of 5' end seq. 594
3' end seq. ID SHF243Z
3' end seq. 
>SHF243Z.Seq
NNNNNNNNNNGGAAGGTAAAGNATGAATTCNAGTTACAAAAGGTTCCNANTTTAGNCNCN
CATCAGGAAAAGATTACCTNTGTTATCCANNTAGAGGATAATGTNTTATCGGG
Length of 3' end seq. 113
Connected seq. ID SHF243P
Connected seq. 
>SHF243P.Seq
CATAAAAAACAAATTGAATTAACTCATGCTTGTACAGTTGGTGATGTAAATTATTTAGAT
CAAATTATTTTATCTGGTGTTTCAACAGATCAATTAAAAGAAGCACATAAAGCAGGACAT
GCAATTGTATTTGATTCATTATTAACACATAATAGTAATTCATTAGTTGTTACAGGTACA
ATGGCATTATTAGGAGCGATTGTAGAGAATGGAGATAGAAAAGGTAAACGTTTATCATTA
TCAAGATCGAATTTATCTAGATTCCCAATGTCGATAACACAAATGTGTACTCATTTGGTT
GAGTTAGATTTAAGTGATAATAAAATCACCGAATTACCAAAGGATATCCAATTGTTAAAG
TCTTTGAGAATTTTAATTTTACGTGGTAATCTATTGGAAGATATACCATTGGAGATTTGT
TATTTAGGAGATTTAAAGATTTTAGAGTTACAAGAAAATCCATTGAATAATTTCCCATTG
AGTGTTGTTCAAAGTGGTACAAAGAATCTTTTATTATTTTGTAAGAATATATTGGAGCGT
AAAAAGAGTGAAACTTGGAATAAGGTCAAGTTGATGTTTGTTGG----------GGAAGG
TAAAGNATGAATTCNAGTTACAAAAGGTTCCNANTTTAGNCNCNCATCAGGAAAAGATTA
CCTNTGTTATCCANNTAGAGGATAATGTNTTATCGGG
Length of connected seq. 687
Full length Seq ID -
Full length Seq. -
Length of full length seq. -