>SHF238 (SHF238Q) /CSM/SH/SHF2-B/SHF238Q.Seq.d/
ATTCTTCTTACAAATTATCAAATATAGAAAAAAAAAATAATAATAATAATATATAACTTA
CTATAGAAATAAAAAACATTTCAAAAAATAAATTTCTTAAGGAGGAAACCAAGGATAAAA
AAAAAAXXXXXXXXXXTGAAGAGTCGGGTATTCCAAGATTCTCAATTGTAAATGCCGTTG
GTTCATTGATTGCAAGAAGTTCAATTTGTGGTGTCTATTTAAATGCAGGTCGTGAACATG
CTGTAGCTTCAACCAAAGCATTCTCATCACAAGTTACAGTATTATCATTGGTTGCAAATT
GGTTCGCTCAACATAGAGGCATCGAAGAGGGTAAAAGACGTCAGTTAATCGATTCTCTTC
ATCGTTTACCAACAAATTTGGGTATGGCATTACGTGTTCGTGAACAATGTAAAGCAATCG
CTCAAAAAATTTATAAAAAGAATTCAATCTTTGTTTTGGGTAAAGGTTTCGCTGAACCAA
TCGCATTTGAAGGTGCTTTGAAAATTAAAGAAATCACCTATTGTCATGCTGAAGGTTATA
GTGGTGGTGCTCTTAAACATGGTCCATTCGCTCTCATTGAACAAGATACACCAATTATCT
TGATCATTTTAGAATGATAGTAATTCTCCATTAATGAGAGTCGC

ssyklsniekknnnnni*LTIEIKNISKNKFLKEETKDKKK---

---EESGIPRFSIVNAVGSLIARSSICGVYLNAGREHAVASTKAFSSQVTVLSLVANWFA
QHRGIEEGKRRQLIDSLHRLPTNLGMALRVREQCKAIAQKIYKKNSIFVLGKGFAEPIAF
EGALKIKEITYCHAEGYSGGALKHGPFALIEQDTPIILIILE***fsinesr

Frame A:
illtnyqi*kkkiiiiiynll*k*ktfqkinflrrkprikkk---

---*rvgyskilnckcrwfidckkfnlwclfkcrs*tccsfnqsilitsysiiigcklvr
st*rhrrg*ktsvnrfsssftnkfgygitcs*tm*snrsknl*kefnlcfg*rfr*tnri
*rcfen*rnhllsc*rl*wwcs*twsirsh*trytnyldhfrmivilh**es

Frame B:
fflqiikyrkkk****yityyrnkkhfkk*is*ggnqg*kk---

---EESGIPRFSIVNAVGSLIARSSICGVYLNAGREHAVASTKAFSSQVTVLSLVANWFA
QHRGIEEGKRRQLIDSLHRLPTNLGMALRVREQCKAIAQKIYKKNSIFVLGKGFAEPIAF
EGALKIKEITYCHAEGYSGGALKHGPFALIEQDTPIILIILE***fsinesr

Frame C:
ssyklsniekknnnnni*LTIEIKNISKNKFLKEETKDKKK---

---ksrvfqdsql*mplvh*lqevqfvvsi*mqvvnml*lqpkhshhklqyyhwlqigsl
nieaskrvkdvs*silfivyqqiwvwhyvfvnnvkqslkkfikriqslfwvkvslnqshl
kvl*klkkspivmlkvivvvllnmvhslslnkihqls*sf*ndsnsplmrv

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHF238 (SHF238Q) /CSM/SH/SHF2-B/SHF238Q.Seq.d/                       1007   0.0
VHB294 (VHB294Q) /CSM/VH/VHB2-D/VHB294Q.Seq.d/                        991   0.0
VFD470 (VFD470Q) /CSM/VF/VFD4-C/VFD470Q.Seq.d/                        991   0.0
SLH483 (SLH483Q) /CSM/SL/SLH4-D/SLH483Q.Seq.d/                        979   0.0
CHH213 (CHH213Q) /CSM/CH/CHH2-A/CHH213Q.Seq.d/                        979   0.0
AFK688 (AFK688Q) /CSM/AF/AFK6-D/AFK688Q.Seq.d/                        977   0.0
CHM485 (CHM485Q) /CSM/CH/CHM4-D/CHM485Q.Seq.d/                        969   0.0
CFC248 (CFC248Q) /CSM/CF/CFC2-B/CFC248Q.Seq.d/                        965   0.0
SFE659 (SFE659Q) /CSM/SF/SFE6-C/SFE659Q.Seq.d/                        963   0.0
CHR457 (CHR457Q) /CSM/CH/CHR4-C/CHR457Q.Seq.d/                        961   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF310886|AF310886.1 Dictyostelium discoideum RacA (racA) gene, complete cds.    54   5e-09  2
BX957223|BX957223.1 Methanococcus maripaludis S2 complete genome; segment 5/5.    50   0.062  1
BI746638|BI746638.1 rm29g07.y1 Meloidogyne arenaria egg pAMP1 v1 Chiapelli McCarter Meloidogyne arenaria cDNA 5' similar to TR:Q19130 Q19130 F07A11.2 PROTEIN. [1] ;, mRNA sequence.    36   0.097  2
BM169673|BM169673.1 EST572196 PyBS Plasmodium yoelii yoelii cDNA clone PYCPU83 5' end, mRNA sequence.    36   0.18   2
AJ723845|AJ723845.1 Gallus gallus EST, clone 26h2s7.               46   0.97   1
AJ723830|AJ723830.1 Gallus gallus EST, clone 26h2r4.               46   0.97   1
BU467478|BU467478.1 603373570F1 CSEQRBN20 Gallus gallus cDNA clone ChEST284k17 5', mRNA sequence.    46   0.97   1
BU463928|BU463928.1 603777177F1 CSEQRBN19 Gallus gallus cDNA clone ChEST719j19 5', mRNA sequence.    46   0.97   1
BU445833|BU445833.1 603766675F1 CSEQRBN13 Gallus gallus cDNA clone ChEST693f20 5', mRNA sequence.    46   0.97   1
BU394218|BU394218.1 603512587F1 CSEQCHN57 Gallus gallus cDNA clone ChEST447m21 5', mRNA sequence.    46   0.97   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM910988_95(AM910988|pid:none) Plasmodium knowlesi strain H chro...   146   5e-34
CP000112_502(CP000112|pid:none) Desulfovibrio desulfuricans G20,...   134   3e-30
CP000777_489(CP000777|pid:none) Leptospira biflexa serovar Patoc...   132   1e-29
BC160581_1(BC160581|pid:none) Xenopus tropicalis glutamine-fruct...   131   1e-29
CP001097_87(CP001097|pid:none) Chlorobium limicola DSM 245, comp...   130   3e-29
BC043809_1(BC043809|pid:none) Xenopus laevis glutamine fructose-...   130   3e-29
CP001101_96(CP001101|pid:none) Chlorobium phaeobacteroides BS1, ...   129   7e-29
AE014297_4394(AE014297|pid:none) Drosophila melanogaster chromos...   129   9e-29
CP000108_5(CP000108|pid:none) Chlorobium chlorochromatii CaD3, c...   129   9e-29
BC163281_1(BC163281|pid:none) Danio rerio glutamine-fructose-6-p...   128   1e-28

 psg: 0.43  gvh: 0.46  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for SHF238 is nuc

>SHF238F.Seq
ATTCTTCTTACAAATTATCAAATATAGAAAAAAAAAATAATAATAATAATATATAACTTA
CTATAGAAATAAAAAACATTTCAAAAAATAAATTTCTTAAGGAGGAAACCAAGGATAAAA
AAAAAANNNNNNNNNN

>SHF238Z.Seq
NNNNNNNNNNTGAAGAGTCGGGTATTCCAAGATTCTCAATTGTAAATGCCGTTGGTTCAT
TGATTGCAAGAAGTTCAATTTGTGGTGTCTATTTAAATGCAGGTCGTGAACATGCTGTAG
CTTCAACCAAAGCATTCTCATCACAAGTTACAGTATTATCATTGGTTGCAAATTGGTTCG
CTCAACATAGAGGCATCGAAGAGGGTAAAAGACGTCAGTTAATCGATTCTCTTCATCGTT
TACCAACAAATTTGGGTATGGCATTACGTGTTCGTGAACAATGTAAAGCAATCGCTCAAA
AAATTTATAAAAAGAATTCAATCTTTGTTTTGGGTAAAGGTTTCGCTGAACCAATCGCAT
TTGAAGGTGCTTTGAAAATTAAAGAAATCACCTATTGTCATGCTGAAGGTTATAGTGGTG
GTGCTCTTAAACATGGTCCATTCGCTCTCATTGAACAAGATACACCAATTATCTTGATCA
TTTTAGAATGATAGTAATTCTCCATTAATGAGAGTCGC

>SHF238P.Seq
ATTCTTCTTACAAATTATCAAATATAGAAAAAAAAAATAATAATAATAATATATAACTTA
CTATAGAAATAAAAAACATTTCAAAAAATAAATTTCTTAAGGAGGAAACCAAGGATAAAA
AAAAAA----------TGAAGAGTCGGGTATTCCAAGATTCTCAATTGTAAATGCCGTTG
GTTCATTGATTGCAAGAAGTTCAATTTGTGGTGTCTATTTAAATGCAGGTCGTGAACATG
CTGTAGCTTCAACCAAAGCATTCTCATCACAAGTTACAGTATTATCATTGGTTGCAAATT
GGTTCGCTCAACATAGAGGCATCGAAGAGGGTAAAAGACGTCAGTTAATCGATTCTCTTC
ATCGTTTACCAACAAATTTGGGTATGGCATTACGTGTTCGTGAACAATGTAAAGCAATCG
CTCAAAAAATTTATAAAAAGAATTCAATCTTTGTTTTGGGTAAAGGTTTCGCTGAACCAA
TCGCATTTGAAGGTGCTTTGAAAATTAAAGAAATCACCTATTGTCATGCTGAAGGTTATA
GTGGTGGTGCTCTTAAACATGGTCCATTCGCTCTCATTGAACAAGATACACCAATTATCT
TGATCATTTTAGAATGATAGTAATTCTCCATTAATGAGAGTCGC