>SHF234 (SHF234Q) /CSM/SH/SHF2-B/SHF234Q.Seq.d/
AGGACCTTATTACTTAAAGATGACAACAAATAATCCATTTGATAAAGTAAAGGATGTATT
AAAATCACAAGATCAAACTTATAATTTTTATAATCTTTCAAAACTTCAAGACCCAAGAAT
TGAAAAATTACCATACTCCATTAGAATTCTTTTGGAATCAGCAGTTCGTAATTGTGATAA
CTTTGAAGTACACGAAAAAGATGTTGAAAATATCTTAAATTGGGAAAACACTGCAAATAA
AGTTGAAATTCCTTTCAAACCAGCAAGAGTATTATTACAAGATTTTACTGGTGTACCAGC
AGTTGTTGATCTTGCAGCAATGAGAGATGCCATGAAAAGATTAGGTGGTGATCCAGCAAA
AATTAACCCACTTGTTCCAGTTGATTTAGTTATTGATCACTCTGTTCAAGTTGATGTATC
AAGAACTGTTGATGCTTTAGAGCAAAATCAAAAGATTGAATTCCATAGAAATCATGAAAG
ATTTTCATTTTTAAAATGGGGTGCTCAAGCATTTGATGGATTATTTATTGCTCCACCTGG
AAGTGGTATTGTACATCAAGTAAATTTAGAGTATATTGCAAGAGAAGTTATGAATGGAAC
TGGTAACCTCCTATATCCGGATAXXXXXXXXXXTCTTGGTGATAGTATTACCACTGATCA
TATTTCACCAGCCGGTAACATCAATAGAAAATCATCAGCCGCTCGTTATTTAGAGTCCAA
AGGTGTAAAACCAGAAGATTTTAACACCTATGGATCTCGTAGAGGTAATGATGAAATTAT
GGTTAGAGGTACCTTTGCCAATACTCGTATTGTTAATAAATTGGCTCCAGCCGTTGGTCC
ACAAACAACNTATGTACCAACTGGTGAGTTAATGTTTATTTCAGATGCTGCAGAAAAATA
TCAATCAGAAGGTCATCAACTCATTGTTTTGGCTGGTAGTGATTATGGCTCTGGTAGTTC
TAGAGATTGGGCTGCCAAAGGTCCATACCTTCAAGGAATTAAGTGTGTCATTGCCATCTC
ATTTGAACGTATTCATAGATCCAATCTCGTTGGTATGGGTATTATCCCATTGCAATTCCA
ACCAGGTCAAAATGCCTCTACTTTGGGTCTCACTGGTAAAGAACAATTCAATATCGAGTT
ACCAACTGATAAATCCNTCATTAAAACTGGTCAAACCGTAAAGGTTACCACTAATTGTGG
TAAATCTTTTGAAACTATCCTTCGTTTCGATACTCCAATCGAAGTTGAATATTGGGCAAA
TAATGGTATCTTATCTTATGTTTTAAGAAAACTTTTACATTAATTTCTTTTTTTAATATA
AAATAAAAAAAAATAATATTATTTAAAA

GPYYLKMTTNNPFDKVKDVLKSQDQTYNFYNLSKLQDPRIEKLPYSIRILLESAVRNCDN
FEVHEKDVENILNWENTANKVEIPFKPARVLLQDFTGVPAVVDLAAMRDAMKRLGGDPAK
INPLVPVDLVIDHSVQVDVSRTVDALEQNQKIEFHRNHERFSFLKWGAQAFDGLFIAPPG
SGIVHQVNLEYIAREVMNGTGNLLYPD---

---LGDSITTDHISPAGNINRKSSAARYLESKGVKPEDFNTYGSRRGNDEIMVRGTFANT
RIVNKLAPAVGPQTTYVPTGELMFISDAAEKYQSEGHQLIVLAGSDYGSGSSRDWAAKGP
YLQGIKCVIAISFERIHRSNLVGMGIIPLQFQPGQNASTLGLTGKEQFNIELPTDKSXIK
TGQTVKVTTNCGKSFETILRFDTPIEVEYWANNGILSYVLRKLLH*flfli*nkkk*yyl
k

Frame A:
rtlllkddnk*si**skgcikitrsnl*fl*sfktsrpkn*kitilh*nsfgisss*l**
l*strkrc*kylklgkhck*s*nsfqtsksiitrfywctssc*scsnerchekirw*ssk
n*ptcss*fsy*slcss*ciknc*cfrakskd*ip*ks*kififkmgcssi*wiiycstw
kwyctsskfrvyckrsyewnw*ppisg---

---sw**yyh*syftsr*hq*kiisrslfrvqrcktrrf*hlwis*r***nyg*rylcqy
syc**igssrwstnnxctnw*vnvyfrccrkisirrssthcfgw**lwlw*f*rlgcqrs
ipsrn*vchchli*tys*iqsrwygyypiaiptrskclyfgshw*rtiqyrvtn**ixh*
nwsnrkgyh*lw*if*nypsfrysnrs*ilgk*wylilcfkktftlisffnik*kkiilf
k

Frame B:
GPYYLKMTTNNPFDKVKDVLKSQDQTYNFYNLSKLQDPRIEKLPYSIRILLESAVRNCDN
FEVHEKDVENILNWENTANKVEIPFKPARVLLQDFTGVPAVVDLAAMRDAMKRLGGDPAK
INPLVPVDLVIDHSVQVDVSRTVDALEQNQKIEFHRNHERFSFLKWGAQAFDGLFIAPPG
SGIVHQVNLEYIAREVMNGTGNLLYPD---

---LGDSITTDHISPAGNINRKSSAARYLESKGVKPEDFNTYGSRRGNDEIMVRGTFANT
RIVNKLAPAVGPQTTYVPTGELMFISDAAEKYQSEGHQLIVLAGSDYGSGSSRDWAAKGP
YLQGIKCVIAISFERIHRSNLVGMGIIPLQFQPGQNASTLGLTGKEQFNIELPTDKSXIK
TGQTVKVTTNCGKSFETILRFDTPIEVEYWANNGILSYVLRKLLH*flfli*nkkk*yyl
k

Frame C:
dlit*r*qqiihlik*rmy*nhkikliifiifqnfktqelknyhtpleffwnqqfvivit
lkytkkmlkis*igktlqiklkflsnqqeyyykillvyqqllilqq*emp*kd*vviqqk
lthlfqli*llitlfklmyqellml*skikrlnsieimkdfhf*ngvlkhlmdylllhle
vvlyik*i*silqekl*melvtsyiri---

---lvivlpliifhqpvtsienhqplvi*spkv*nqkiltpmdlvevmmklwlevplpil
vllinwlqplvhkqxmyqlvs*clfqmlqkninqkvinslfwlvvimalvvleiglpkvh
tfkelsvslpshlnvfidpislvwvlshcnsnqvkmpllwvslvknnsissyqlinpslk
lvkp*rlplivvnllklsfvsilqsklnigqimvsylmf*enfyinfff*ykikknnii*

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHF234 (SHF234Q) /CSM/SH/SHF2-B/SHF234Q.Seq.d/                       2553   0.0
VHQ461 (VHQ461Q) /CSM/VH/VHQ4-C/VHQ461Q.Seq.d/                       1358   0.0
VHQ432 (VHQ432Q) /CSM/VH/VHQ4-B/VHQ432Q.Seq.d/                       1358   0.0
VHQ267 (VHQ267Q) /CSM/VH/VHQ2-C/VHQ267Q.Seq.d/                       1358   0.0
VHO844 (VHO844Q) /CSM/VH/VHO8-B/VHO844Q.Seq.d/                       1358   0.0
VHO678 (VHO678Q) /CSM/VH/VHO6-D/VHO678Q.Seq.d/                       1358   0.0
VHA714 (VHA714Q) /CSM/VH/VHA7-A/VHA714Q.Seq.d/                       1358   0.0
SHL826 (SHL826Q) /CSM/SH/SHL8-B/SHL826Q.Seq.d/                       1358   0.0
SHL632 (SHL632Q) /CSM/SH/SHL6-B/SHL632Q.Seq.d/                       1358   0.0
SHL453 (SHL453Q) /CSM/SH/SHL4-C/SHL453Q.Seq.d/                       1358   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF194945|AF194945.1 Nicotiana tabacum cytosolic aconitase (ACO) mRNA, complete cds.    66   9e-34  6
AY250116|AY250116.1 Lycopersicon pennellii aconitase mRNA, partial cds.    60   6e-26  5
AY250115|AY250115.1 Lycopersicon pennellii aconitase mRNA, partial cds.    60   2e-21  5
BE579347|BE579347.1 kq26c09.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to WP:ZK455.1 CE03812 PROBABLE ACONITATE HYDRATASE, CYTOPLASMIC ;, mRNA sequence.    64   2e-20  3
BI925204|BI925204.1 EST545093 tomato flower, buds 0-3 mm Lycopersicon esculentum cDNA clone cTOA25H12 5' end, mRNA sequence.    58   7e-19  3
BI930520|BI930520.1 EST550409 tomato flower, 8 mm to preanthesis buds Lycopersicon esculentum cDNA clone cTOC16J14 5' end, mRNA sequence.    58   8e-19  3
CO910019|CO910019.1 BJ02055G09 BJ02 Capsicum annuum cDNA 5', mRNA sequence.    58   2e-18  4
CK265172|CK265172.1 EST711250 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POABX20 5' end, mRNA sequence.    58   1e-17  4
AY062772|AY062772.1 Arabidopsis thaliana Unknown protein (At4g35830; F4B14.100) mRNA, complete cds.    46   6e-17  6
BQ511380|BQ511380.2 EST618795 Generation of a set of potato cDNA clones for microarray analyses mixed potato tissues Solanum tuberosum cDNA clone STMHR19 5' end, mRNA sequence.    58   9e-16  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54X73) RecName: Full=Probable cytoplasmic aconitate hydratase;...   445   e-123
AB236788_1(AB236788|pid:none) Trifolium pratense RNA for putativ...   295   3e-78
AP008209_256(AP008209|pid:none) Oryza sativa (japonica cultivar-...   293   9e-78
(Q23500) RecName: Full=Probable cytoplasmic aconitate hydratase;...   291   3e-77
BT060820_1(BT060820|pid:none) Zea mays full-length cDNA clone ZM...   291   3e-77
BT060665_1(BT060665|pid:none) Zea mays full-length cDNA clone ZM...   290   6e-77
DQ294230_1(DQ294230|pid:none) Glossina morsitans morsitans TC165...   288   2e-76
(P49608) RecName: Full=Aconitate hydratase, cytoplasmic;        ...   287   5e-76
(Q9SIB9) RecName: Full=Aconitate hydratase 2, mitochondrial;    ...   286   1e-75
AY050485_1(AY050485|pid:none) Prunus avium putative aconitase mR...   285   2e-75

 psg: 0.66  gvh: 0.34  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: cytoskeletal

>> prediction for SHF234 is cyt

>SHF234F.Seq
AGGACCTTATTACTTAAAGATGACAACAAATAATCCATTTGATAAAGTAAAGGATGTATT
AAAATCACAAGATCAAACTTATAATTTTTATAATCTTTCAAAACTTCAAGACCCAAGAAT
TGAAAAATTACCATACTCCATTAGAATTCTTTTGGAATCAGCAGTTCGTAATTGTGATAA
CTTTGAAGTACACGAAAAAGATGTTGAAAATATCTTAAATTGGGAAAACACTGCAAATAA
AGTTGAAATTCCTTTCAAACCAGCAAGAGTATTATTACAAGATTTTACTGGTGTACCAGC
AGTTGTTGATCTTGCAGCAATGAGAGATGCCATGAAAAGATTAGGTGGTGATCCAGCAAA
AATTAACCCACTTGTTCCAGTTGATTTAGTTATTGATCACTCTGTTCAAGTTGATGTATC
AAGAACTGTTGATGCTTTAGAGCAAAATCAAAAGATTGAATTCCATAGAAATCATGAAAG
ATTTTCATTTTTAAAATGGGGTGCTCAAGCATTTGATGGATTATTTATTGCTCCACCTGG
AAGTGGTATTGTACATCAAGTAAATTTAGAGTATATTGCAAGAGAAGTTATGAATGGAAC
TGGTAACCTCCTATATCCGGATANNNNNNNNNN

>SHF234Z.Seq
NNNNNNNNNNTCTTGGTGATAGTATTACCACTGATCATATTTCACCAGCCGGTAACATCA
ATAGAAAATCATCAGCCGCTCGTTATTTAGAGTCCAAAGGTGTAAAACCAGAAGATTTTA
ACACCTATGGATCTCGTAGAGGTAATGATGAAATTATGGTTAGAGGTACCTTTGCCAATA
CTCGTATTGTTAATAAATTGGCTCCAGCCGTTGGTCCACAAACAACNTATGTACCAACTG
GTGAGTTAATGTTTATTTCAGATGCTGCAGAAAAATATCAATCAGAAGGTCATCAACTCA
TTGTTTTGGCTGGTAGTGATTATGGCTCTGGTAGTTCTAGAGATTGGGCTGCCAAAGGTC
CATACCTTCAAGGAATTAAGTGTGTCATTGCCATCTCATTTGAACGTATTCATAGATCCA
ATCTCGTTGGTATGGGTATTATCCCATTGCAATTCCAACCAGGTCAAAATGCCTCTACTT
TGGGTCTCACTGGTAAAGAACAATTCAATATCGAGTTACCAACTGATAAATCCNTCATTA
AAACTGGTCAAACCGTAAAGGTTACCACTAATTGTGGTAAATCTTTTGAAACTATCCTTC
GTTTCGATACTCCAATCGAAGTTGAATATTGGGCAAATAATGGTATCTTATCTTATGTTT
TAAGAAAACTTTTACATTAATTTCTTTTTTTAATATAAAATAAAAAAAAATAATATTATT
TAAAA

>SHF234P.Seq
AGGACCTTATTACTTAAAGATGACAACAAATAATCCATTTGATAAAGTAAAGGATGTATT
AAAATCACAAGATCAAACTTATAATTTTTATAATCTTTCAAAACTTCAAGACCCAAGAAT
TGAAAAATTACCATACTCCATTAGAATTCTTTTGGAATCAGCAGTTCGTAATTGTGATAA
CTTTGAAGTACACGAAAAAGATGTTGAAAATATCTTAAATTGGGAAAACACTGCAAATAA
AGTTGAAATTCCTTTCAAACCAGCAAGAGTATTATTACAAGATTTTACTGGTGTACCAGC
AGTTGTTGATCTTGCAGCAATGAGAGATGCCATGAAAAGATTAGGTGGTGATCCAGCAAA
AATTAACCCACTTGTTCCAGTTGATTTAGTTATTGATCACTCTGTTCAAGTTGATGTATC
AAGAACTGTTGATGCTTTAGAGCAAAATCAAAAGATTGAATTCCATAGAAATCATGAAAG
ATTTTCATTTTTAAAATGGGGTGCTCAAGCATTTGATGGATTATTTATTGCTCCACCTGG
AAGTGGTATTGTACATCAAGTAAATTTAGAGTATATTGCAAGAGAAGTTATGAATGGAAC
TGGTAACCTCCTATATCCGGATA----------TCTTGGTGATAGTATTACCACTGATCA
TATTTCACCAGCCGGTAACATCAATAGAAAATCATCAGCCGCTCGTTATTTAGAGTCCAA
AGGTGTAAAACCAGAAGATTTTAACACCTATGGATCTCGTAGAGGTAATGATGAAATTAT
GGTTAGAGGTACCTTTGCCAATACTCGTATTGTTAATAAATTGGCTCCAGCCGTTGGTCC
ACAAACAACNTATGTACCAACTGGTGAGTTAATGTTTATTTCAGATGCTGCAGAAAAATA
TCAATCAGAAGGTCATCAACTCATTGTTTTGGCTGGTAGTGATTATGGCTCTGGTAGTTC
TAGAGATTGGGCTGCCAAAGGTCCATACCTTCAAGGAATTAAGTGTGTCATTGCCATCTC
ATTTGAACGTATTCATAGATCCAATCTCGTTGGTATGGGTATTATCCCATTGCAATTCCA
ACCAGGTCAAAATGCCTCTACTTTGGGTCTCACTGGTAAAGAACAATTCAATATCGAGTT
ACCAACTGATAAATCCNTCATTAAAACTGGTCAAACCGTAAAGGTTACCACTAATTGTGG
TAAATCTTTTGAAACTATCCTTCGTTTCGATACTCCAATCGAAGTTGAATATTGGGCAAA
TAATGGTATCTTATCTTATGTTTTAAGAAAACTTTTACATTAATTTCTTTTTTTAATATA
AAATAAAAAAAAATAATATTATTTAAAA