>SHF219 (SHF219Q) /CSM/SH/SHF2-A/SHF219Q.Seq.d/
ATTGTGTGATTGTGTTGTGTGTGTATTTTGGTTGATACACATTATATATATATATAATAC
AATAAAATGTCATTTTTTTTAGGACAGATTTTAGAAAAGATGGGTTCCATCGATAAAGAT
ATTAGATTCATGGCAACTCATGATCTTGCAAATGAATTAGAAAAAGATACTTTTAAAATG
GATCCAACTTATGAAAATAAAATTGTTACAAAATTATTAGCATTAACAGCTGATTCAGCA
AATAATGTTCAAGAAAATGTTGTTAAATGTTTAGGTTTATTAATTAAAAGAGTTAAAGAT
TCACAAGCAACAGAAATTATTGATACATTATCAAAAAATATTTTAGAAGAATCAAATAAA
GAAGAATTAGTTGAAATTTCAGGTATTGGTTTAAAAACAATCATTACAAATTTACCAAGT
GAAGGTAGTTCAATTTCAACTTTAGTAATTAAAAATTTAGTACCAAAATTATTAATTGGT
ATTGATAGTGAAAAATTAAAAGATAAAAATGAAATTAAAATGTCATGTTTAGATATTTTA
AATGATTTATTACAAAAGTATGGATCATTTATGATTGGAGATTTAGAGAATATTCAAAAG
GTAGTATTACCAAAATTGAATGCAACTXXXXXXXXXXTCTCAATCATGGAGAATAAAGAA
GTTGTCGATCAATATTTAGCTCCAAACATCTCTCAATTCCTTTCACTCTTACATGATGGT
GATTTAATTGTTAGACGTAGTGCCNTCCTTTCATTGAATTACATTGCTCACAATAAACCA
AATCTCATTCGTAATGATCTCTCTGTCTATTTACCAATCTTGTACAACAATGCTAAAATC
AAACCAGAACTCATTCGTGAAGTTGATCTTGGTCCATTCAAACATAAGGTCGATGATGGT
ATTGAAATCAGAAAAACAGCTTTCGAATGTATGTACACTCTTTTGGATACCTCTATCGAT
AAGATCGATGTAGCTCCATTCATCGTTTCATTATGTGATGGTCTCAAAGATACCCAATAC
GATATCAAATTGTTATGTCATTTAATGATCATTCGTTTAGCAAACTCAAACGGTGCTGCT
CTCCTCGAAAATATTACTCTCCTCCTTGAACCACTTCGTGTTATTNTCATGANTAAAGTA
AATGAAACCGCTGTTAAACAACAAATCGAACGTAATGAAGAATGTATTCGTTCAGCTTTA
CGTGCTGTCGCTTCCATCTCTAGAATTCCAAATAGTGATAGTATCGTTAAATTTGAAGAA
TTTGTTAAAAATACAATTCGTACAACTCCATTAGCTGCTCAATTTAATTCAATTTTATAC
GAAGATACAATGTCAAATCAAGATTCA

iv*lccvcilvdthyiyi*YNKMSFFLGQILEKMGSIDKDIRFMATHDLANELEKDTFKM
DPTYENKIVTKLLALTADSANNVQENVVKCLGLLIKRVKDSQATEIIDTLSKNILEESNK
EELVEISGIGLKTIITNLPSEGSSISTLVIKNLVPKLLIGIDSEKLKDKNEIKMSCLDIL
NDLLQKYGSFMIGDLENIQKVVLPKLNAT---

---SIMENKEVVDQYLAPNISQFLSLLHDGDLIVRRSAXLSLNYIAHNKPNLIRNDLSVY
LPILYNNAKIKPELIREVDLGPFKHKVDDGIEIRKTAFECMYTLLDTSIDKIDVAPFIVS
LCDGLKDTQYDIKLLCHLMIIRLANSNGAALLENITLLLEPLRVIXMXKVNETAVKQQIE
RNEECIRSALRAVASISRIPNSDSIVKFEEFVKNTIRTTPLAAQFNSILYEDTMSNQDS

Frame A:
iv*lccvcilvdthyiyi*YNKMSFFLGQILEKMGSIDKDIRFMATHDLANELEKDTFKM
DPTYENKIVTKLLALTADSANNVQENVVKCLGLLIKRVKDSQATEIIDTLSKNILEESNK
EELVEISGIGLKTIITNLPSEGSSISTLVIKNLVPKLLIGIDSEKLKDKNEIKMSCLDIL
NDLLQKYGSFMIGDLENIQKVVLPKLNAT---

---sqswrikklsini*lqtslnsfhsymmvi*lldvvpsfh*itlltinqisfvmisls
iyqscttmlksnqnsfvklilvhsnirsmmvlksekqlsnvctlfwiplsirsm*lhssf
hyvmvskipntisncyvi**sfv*qtqtvllsskillsslnhfvlxs*xk*mkpllnnks
nvmknvfvqlyvlslpslefqivivslnlknllkiqfvqlh*llnliqfytkiqcqiki

Frame B:
lcdcvvcvfwlihiiyiyntikchff*drf*krwvpsikildswqlmilqmn*kkillkw
iqlmkikllqny*h*qliqqimfkkmllnv*vy*lkelkihkqqkllihyqkif*knqik
kn*lkfqvlv*kqslqiyqvkvvqfql**lki*yqny*lvlivkn*kikmklkchv*if*
miyyksmdhl*lei*rifkr*yyqn*mq---

---lnhge*rscrsifsskhlsipftlt*w*fnc*t*cxpfielhcsq*tkshs**slcl
ftnlvqqc*nqtrths*s*swsiqt*gr*wy*nqknsfrmyvhsfgylyr*drcssihrf
im*wsqrypiryqivmsfndhsfsklkrccsprkyyspp*ttscyxhx*sk*nrc*ttnr
t**rmysfsftccrfhl*nsk***yr*i*ric*kynsynsiscsi*fnfirrynvksrf

Frame C:
cvivlcvyfg*ytlyiyiiq*nviffrtdfrkdgfhr*ry*ihgns*sck*irkryf*ng
snl*k*ncykiisins*fsk*csrkcc*mfrfin*ks*rftsnrny*yiikkyfrrik*r
ris*nfrywfknnhykftk*r*fnfnfsn*kfstkiinwy***kikr*k*n*nvmfryfk
*fitkvwiiydwrfreyskgsitkiecn---

---SIMENKEVVDQYLAPNISQFLSLLHDGDLIVRRSAXLSLNYIAHNKPNLIRNDLSVY
LPILYNNAKIKPELIREVDLGPFKHKVDDGIEIRKTAFECMYTLLDTSIDKIDVAPFIVS
LCDGLKDTQYDIKLLCHLMIIRLANSNGAALLENITLLLEPLRVIXMXKVNETAVKQQIE
RNEECIRSALRAVASISRIPNSDSIVKFEEFVKNTIRTTPLAAQFNSILYEDTMSNQDS

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHF219 (SHF219Q) /CSM/SH/SHF2-A/SHF219Q.Seq.d/                       1560   0.0
SHF506 (SHF506Q) /CSM/SH/SHF5-A/SHF506Q.Seq.d/                       1380   0.0
VHQ485 (VHQ485Q) /CSM/VH/VHQ4-D/VHQ485Q.Seq.d/                       1366   0.0
VHB862 (VHB862Q) /CSM/VH/VHB8-C/VHB862Q.Seq.d/                       1366   0.0
SHL726 (SHL726Q) /CSM/SH/SHL7-B/SHL726Q.Seq.d/                       1366   0.0
SHJ830 (SHJ830Q) /CSM/SH/SHJ8-B/SHJ830Q.Seq.d/                       1366   0.0
SHJ681 (SHJ681Q) /CSM/SH/SHJ6-D/SHJ681Q.Seq.d/                       1366   0.0
CHM192 (CHM192Q) /CSM/CH/CHM1-D/CHM192Q.Seq.d/                       1366   0.0
CHF870 (CHF870Q) /CSM/CH/CHF8-C/CHF870Q.Seq.d/                       1366   0.0
AHR372 (AHR372Q) /CSM/AH/AHR3-C/AHR372Q.Seq.d/                       1366   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.  1366   0.0    1
CC114176|CC114176.1 NDL.57K9.SP6 Notre Dame Liverpool Aedes aegypti genomic clone NDL.57K9, DNA sequence.    40   0.007  2
BI888273|BI888273.1 ZF637-2-000028 Zebrafish shield stage whole embryo cDNA library MPMGp637 Danio rerio cDNA clone MPMGp637_18D5;MPMGp637D0518 5', mRNA sequence.    52   0.033  1
CT573216|CT573216.1 Medicago truncatula chromosome 5 clone mte1-79f1, COMPLETE SEQUENCE.    36   0.090  7
CF446836|CF446836.1 EST683181 normalized cDNA library of onion Allium cepa cDNA clone ACAAS70, mRNA sequence.    50   0.13   1
AC130470|AC130470.2 Homo sapiens chromosome 16 clone RP11-143P24, complete sequence.    38   0.15   3
DU016203|DU016203.1 238566 Tomato MboI BAC Library Lycopersicon esculentum genomic clone SL_MboI0039H16 5, genomic survey sequence.    36   0.16   3
AE014832|AE014832.1 Plasmodium falciparum 3D7 chromosome 10 section 4 of 7 of the complete sequence.    36   0.28   2
BB976652|BB976652.1 Plasmodium berghei strain ANKA cDNA clone:OK006559, ookinete 5' EST.    46   0.44   2
BE555247|BE555247.1 sp87b08.y1 Gm-c1045 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1045-592 5' similar to TR:O64720 O64720 PUTATIVE TBP-BINDING PROTEIN. ;, mRNA sequence.    38   0.51   2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q86KD1) RecName: Full=Cullin-associated NEDD8-dissociated prote...   425   e-117
BT068509_1(BT068509|pid:none) Zea mays full-length cDNA clone ZM...   193   1e-47
BC004232_1(BC004232|pid:none) Homo sapiens cullin-associated and...   187   5e-46
AK001791_1(AK001791|pid:none) Homo sapiens cDNA FLJ10929 fis, cl...   187   5e-46
AB179357_1(AB179357|pid:none) Macaca fascicularis testis cDNA cl...   187   5e-46
BC026220_1(BC026220|pid:none) Homo sapiens cullin-associated and...   187   5e-46
AB020636_1(AB020636|pid:none) Homo sapiens mRNA for KIAA0829 pro...   187   5e-46
AK292456_1(AK292456|pid:none) Homo sapiens cDNA FLJ77762 complet...   187   5e-46
AL833880_1(AL833880|pid:none) Homo sapiens mRNA; cDNA DKFZp434P1...   187   5e-46
(A7MBJ5) RecName: Full=Cullin-associated NEDD8-dissociated prote...   187   5e-46

 psg: 0.90  gvh: 0.44  alm: 0.36  top: 0.40  tms: 0.00  mit: 0.16  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 32.0 %: nuclear
	 12.0 %: vesicles of secretory system
	  8.0 %: endoplasmic reticulum

>> prediction for SHF219 is cyt

>SHF219F.Seq
ATTGTGTGATTGTGTTGTGTGTGTATTTTGGTTGATACACATTATATATATATATAATAC
AATAAAATGTCATTTTTTTTAGGACAGATTTTAGAAAAGATGGGTTCCATCGATAAAGAT
ATTAGATTCATGGCAACTCATGATCTTGCAAATGAATTAGAAAAAGATACTTTTAAAATG
GATCCAACTTATGAAAATAAAATTGTTACAAAATTATTAGCATTAACAGCTGATTCAGCA
AATAATGTTCAAGAAAATGTTGTTAAATGTTTAGGTTTATTAATTAAAAGAGTTAAAGAT
TCACAAGCAACAGAAATTATTGATACATTATCAAAAAATATTTTAGAAGAATCAAATAAA
GAAGAATTAGTTGAAATTTCAGGTATTGGTTTAAAAACAATCATTACAAATTTACCAAGT
GAAGGTAGTTCAATTTCAACTTTAGTAATTAAAAATTTAGTACCAAAATTATTAATTGGT
ATTGATAGTGAAAAATTAAAAGATAAAAATGAAATTAAAATGTCATGTTTAGATATTTTA
AATGATTTATTACAAAAGTATGGATCATTTATGATTGGAGATTTAGAGAATATTCAAAAG
GTAGTATTACCAAAATTGAATGCAACTNNNNNNNNNN

>SHF219Z.Seq
NNNNNNNNNNTCTCAATCATGGAGAATAAAGAAGTTGTCGATCAATATTTAGCTCCAAAC
ATCTCTCAATTCCTTTCACTCTTACATGATGGTGATTTAATTGTTAGACGTAGTGCCNTC
CTTTCATTGAATTACATTGCTCACAATAAACCAAATCTCATTCGTAATGATCTCTCTGTC
TATTTACCAATCTTGTACAACAATGCTAAAATCAAACCAGAACTCATTCGTGAAGTTGAT
CTTGGTCCATTCAAACATAAGGTCGATGATGGTATTGAAATCAGAAAAACAGCTTTCGAA
TGTATGTACACTCTTTTGGATACCTCTATCGATAAGATCGATGTAGCTCCATTCATCGTT
TCATTATGTGATGGTCTCAAAGATACCCAATACGATATCAAATTGTTATGTCATTTAATG
ATCATTCGTTTAGCAAACTCAAACGGTGCTGCTCTCCTCGAAAATATTACTCTCCTCCTT
GAACCACTTCGTGTTATTNTCATGANTAAAGTAAATGAAACCGCTGTTAAACAACAAATC
GAACGTAATGAAGAATGTATTCGTTCAGCTTTACGTGCTGTCGCTTCCATCTCTAGAATT
CCAAATAGTGATAGTATCGTTAAATTTGAAGAATTTGTTAAAAATACAATTCGTACAACT
CCATTAGCTGCTCAATTTAATTCAATTTTATACGAAGATACAATGTCAAATCAAGATTCA

>SHF219P.Seq
ATTGTGTGATTGTGTTGTGTGTGTATTTTGGTTGATACACATTATATATATATATAATAC
AATAAAATGTCATTTTTTTTAGGACAGATTTTAGAAAAGATGGGTTCCATCGATAAAGAT
ATTAGATTCATGGCAACTCATGATCTTGCAAATGAATTAGAAAAAGATACTTTTAAAATG
GATCCAACTTATGAAAATAAAATTGTTACAAAATTATTAGCATTAACAGCTGATTCAGCA
AATAATGTTCAAGAAAATGTTGTTAAATGTTTAGGTTTATTAATTAAAAGAGTTAAAGAT
TCACAAGCAACAGAAATTATTGATACATTATCAAAAAATATTTTAGAAGAATCAAATAAA
GAAGAATTAGTTGAAATTTCAGGTATTGGTTTAAAAACAATCATTACAAATTTACCAAGT
GAAGGTAGTTCAATTTCAACTTTAGTAATTAAAAATTTAGTACCAAAATTATTAATTGGT
ATTGATAGTGAAAAATTAAAAGATAAAAATGAAATTAAAATGTCATGTTTAGATATTTTA
AATGATTTATTACAAAAGTATGGATCATTTATGATTGGAGATTTAGAGAATATTCAAAAG
GTAGTATTACCAAAATTGAATGCAACT----------TCTCAATCATGGAGAATAAAGAA
GTTGTCGATCAATATTTAGCTCCAAACATCTCTCAATTCCTTTCACTCTTACATGATGGT
GATTTAATTGTTAGACGTAGTGCCNTCCTTTCATTGAATTACATTGCTCACAATAAACCA
AATCTCATTCGTAATGATCTCTCTGTCTATTTACCAATCTTGTACAACAATGCTAAAATC
AAACCAGAACTCATTCGTGAAGTTGATCTTGGTCCATTCAAACATAAGGTCGATGATGGT
ATTGAAATCAGAAAAACAGCTTTCGAATGTATGTACACTCTTTTGGATACCTCTATCGAT
AAGATCGATGTAGCTCCATTCATCGTTTCATTATGTGATGGTCTCAAAGATACCCAATAC
GATATCAAATTGTTATGTCATTTAATGATCATTCGTTTAGCAAACTCAAACGGTGCTGCT
CTCCTCGAAAATATTACTCTCCTCCTTGAACCACTTCGTGTTATTNTCATGANTAAAGTA
AATGAAACCGCTGTTAAACAACAAATCGAACGTAATGAAGAATGTATTCGTTCAGCTTTA
CGTGCTGTCGCTTCCATCTCTAGAATTCCAAATAGTGATAGTATCGTTAAATTTGAAGAA
TTTGTTAAAAATACAATTCGTACAACTCCATTAGCTGCTCAATTTAATTCAATTTTATAC
GAAGATACAATGTCAAATCAAGATTCA