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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHF216
Library SH
(Link to library)
Clone ID SHF216
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10794-1
Original site URL
Representative seq. ID SHF216P
(Link to Original site)
Representative DNA sequence 
>SHF216 (SHF216Q) /CSM/SH/SHF2-A/SHF216Q.Seq.d/
GTCATATCAATAATAAATATTATTACATATACAAAACAAAATTAATAAAACTTAAACTTT
ATTTTTCCCCTACATTGGCCAAACTTTAATTTGCCTTTGTACAATTATTCTCTTTAAACT
CTTATTATTAAAATTCATTTTAAAAACTCTCATTTCTAAAAAAAAACTAAAACAAAAAAA
AAANGGTCAATTTTACAATANATCAAATTCGTTCAATTATGGATCGTCGNGAAAATATCA
AAAATATGTCTGTTATTGCTCATGTTGATCATGGTAAAACTACTCTTACTGATTCATTAA
TTCAAANAGCTGGTATTATTGCANATAAGGTATCTGGTAATGCAGGATATATGTCATGTC
GTCCANATGAACAATTACGTGGXXXXXXXXXXTTATCGTCAGACTTAACTGATGATATTG
ATAATGGTTTGGTGGCTTTTAATCAAGATGTTAAAAGTCGTGCCAATTATCTCTATGATA
AACATTCTTGGGATAAGAATGATGCAGTTAACATTTGGTCATTTGGTCCAGAATATAAGG
GTCCAAATGTTTTAGTTAACAAAACCAAAGGGTGTTCAATATCTTCATGAAATTAAAGAC
TCTATGGTTTCAGCATTCCAATGGATCTCAAATGAAGGTGTATTATGTGGTGAGAAAATG
AGATCGATTCGTTTCAACGTTTATGATGTAATGCTTCATGCCGATGCAATTCATAGAGGT
AGTGGTCAATTTATTCCAACAACTCGTCGTGTAATGTATGCCTCCCAACTATCAGCTCAA
CCAACACTTTTGGAACCAATGTATTTGGTTGATATTAGTACTCCTGAAAGTGGTATTAGT
GGTATCTACTCTGTACTCAATCGTAGAAGAGGTGTTGTCATTGGTGAAGAACGTCGTATT
GGTACTCCATTATTCAATATCAAAGCTTATCTTCCTGTAATGGAATCATTTGGTCTCACT
GCTGATTTACGTTCAAGTACTGCTGGTCAAGCTTTCCCTCAATGTGTTTTTGATCATTGG
AGTTCAATTGGTATCGTTGGTCAAGATAAAAGATCAACTGAAGTAGCTTCATCAATTAGA
AAGAGAAAAGGTTTAAATCCAAAATTACCAAC
sequence update 2002.10.25
Translated Amino Acid sequence 
hinnkyyyiyktkliklklyfsptlakl*fafvqlfslnsyy*nsf*klsflkkn*NKKK
XVNFTIXQIRSIMDRRENIKNMSVIAHVDHGKTTLTDSLIQXAGIIAXKVSGNAGYMSCR
PXEQLR---

---LSSDLTDDIDNGLVAFNQDVKSRANYLYDKHSWDKNDAVNIWSFGPEYKGPNVLVNK
TKGCSISS*n*rlygfsipmdlk*rcimw*eneidsfqrl*cnascrcns*r*wsiysnn
sscnvclptisstntfgtnvfg*y*ys*kwy*wyllctqs*krcchw*rtsywysiiqyq
slsscngiiwshc*ftfkycwssfpsmcf*slefnwyrwsr*kin*ssfin*kekrfksk
itn
Translated Amino Acid sequence (All Frames) 
Frame A:
visiiniitytkqn**nlnfifplhwpnfnlplynysl*tliikihfknshf*kktktkk
kxsilqxikfvqlwivxkiskicllllmlimvkllllih*fkxlvllxirylvmqdichv
vxmnnyv---

---LSSDLTDDIDNGLVAFNQDVKSRANYLYDKHSWDKNDAVNIWSFGPEYKGPNVLVNK
TKGCSISS*n*rlygfsipmdlk*rcimw*eneidsfqrl*cnascrcns*r*wsiysnn
sscnvclptisstntfgtnvfg*y*ys*kwy*wyllctqs*krcchw*rtsywysiiqyq
slsscngiiwshc*ftfkycwssfpsmcf*slefnwyrwsr*kin*ssfin*kekrfksk
itn

Frame B:
syq**illhiqnkinkt*tlffpyigqtliclctiilfkllllkfilktliskkklkqkk
xgqfynxsnsfnygssxkyqkyvcycsc*sw*nysy*finsxswyycx*giw*criyvms
sx*titw---

---yrqt*lmilimvwwllikmlkvvpiisminilgirmmqltfghlvqnirvqmf*ltk
pkgvqylheikdsmvsafqwisnegvlcgekmrsirfnvydvmlhadaihrgsgqfiptt
rrvmyasqlsaqptllepmylvdistpesgisgiysvlnrrrgvvigeerrigtplfnik
aylpvmesfgltadlrsstagqafpqcvfdhwssigivgqdkrstevassirkrkglnpk
lp

Frame C:
hinnkyyyiyktkliklklyfsptlakl*fafvqlfslnsyy*nsf*klsflkkn*NKKK
XVNFTIXQIRSIMDRRENIKNMSVIAHVDHGKTTLTDSLIQXAGIIAXKVSGNAGYMSCR
PXEQLR---

---ivrln**y**wfggf*src*kscqlsl**tflg*e*cs*hlviwsri*gskcfs*qn
qrvfnifmklktlwfqhsngsqmkvyyvvrk*drfvstfmm*cfmpmqfievvvnlfqql
vv*cmppnyqlnqhfwnqciwlilvllkvvlvvstlysiveevlslvknvvlvlhysisk
lifl*wnhlvslliyvqvllvklslnvfliigvqlvslvkikdqlk*lhqlerekv*iqn
yq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHF216 (SHF216Q) /CSM/SH/SHF2-A/SHF216Q.Seq.d/                       1750   0.0
AHM516 (AHM516Q) /CSM/AH/AHM5-A/AHM516Q.Seq.d/                       1419   0.0
AHL348 (AHL348Q) /CSM/AH/AHL3-B/AHL348Q.Seq.d/                       1419   0.0
AHD146 (AHD146Q) /CSM/AH/AHD1-B/AHD146Q.Seq.d/                       1419   0.0
AHB739 (AHB739Q) /CSM/AH/AHB7-B/AHB739Q.Seq.d/                       1419   0.0
AHB514 (AHB514Q) /CSM/AH/AHB5-A/AHB514Q.Seq.d/                       1419   0.0
AHE765 (AHE765Q) /CSM/AH/AHE7-C/AHE765Q.Seq.d/                       1415   0.0
AHB695 (AHB695Q) /CSM/AH/AHB6-D/AHB695Q.Seq.d/                       1415   0.0
AHC654 (AHC654Q) /CSM/AH/AHC6-C/AHC654Q.Seq.d/                       1413   0.0
VHC477 (VHC477Q) /CSM/VH/VHC4-D/VHC477Q.Seq.d/                       1405   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.   131   6e-75  9
X15386|X15386.1 Dictyostelium discoideum H6 gene mRNA.            230   1e-60  2
BI863585|BI863585.1 kx46a06.y1 Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to SW:EF2_CAEEL P29691 ELONGATION FACTOR 2 ;, mRNA sequence.    66   5e-18  4
BI863575|BI863575.1 kx45h07.y1 Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to SW:EF2_CHICK Q90705 ELONGATION FACTOR 2 ;, mRNA sequence.    66   9e-15  3
BI451383|BI451383.1 kx23e09.y1 Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to SW:EF2_CAEEL P29691 ELONGATION FACTOR 2 ;, mRNA sequence.    66   1e-14  3
BM056853|BM056853.1 2154-85 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides felis cDNA clone 2154-85, mRNA sequence.    92   3e-14  1
DR447725|DR447725.1 AR1047E10 A. gomesiana hemocytes normalized library Acanthoscurria gomesiana cDNA clone AR1047E10 5', mRNA sequence.    88   6e-14  2
DR443579|DR443579.1 AR2018A03 A. gomesiana hemocytes library Acanthoscurria gomesiana cDNA clone AR2018A03 5', mRNA sequence.    88   6e-14  2
CX086961|CX086961.1 EHACS56TR E. histolytica Normalized cDNA library Entamoeba histolytica cDNA, mRNA sequence.    46   7e-14  4
BM058578|BM058578.1 2193-94 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides felis cDNA clone 2193-94, mRNA sequence.    90   1e-13  1
dna update 2006. 3.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   216   7e-66
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   215   4e-65
BT072304_1(BT072304|pid:none) Salmo salar clone ssal-rgf-520-280...   217   4e-65
AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   214   7e-65
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   216   9e-65
AK032997_1(AK032997|pid:none) Mus musculus 12 days embryo male w...   215   9e-65
(P05197) RecName: Full=Elongation factor 2;          Short=EF-2;...   215   9e-65
AK159514_1(AK159514|pid:none) Mus musculus osteoclast-like cell ...   215   9e-65
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   215   9e-65
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   215   9e-65
protein update 2009. 4.18
PSORT 

 psg: 0.80  gvh: 0.50  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.33  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 28.0 %: cytoplasmic
	 28.0 %: mitochondrial
	 28.0 %: nuclear
	 12.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum

>> prediction for SHF216 is cyt
5' end seq. ID SHF216F
5' end seq. 
>SHF216F.Seq
GTCATATCAATAATAAATATTATTACATATACAAAACAAAATTAATAAAACTTAAACTTT
ATTTTTCCCCTACATTGGCCAAACTTTAATTTGCCTTTGTACAATTATTCTCTTTAAACT
CTTATTATTAAAATTCATTTTAAAAACTCTCATTTCTAAAAAAAAACTAAAACAAAAAAA
AAANGGTCAATTTTACAATANATCAAATTCGTTCAATTATGGATCGTCGNGAAAATATCA
AAAATATGTCTGTTATTGCTCATGTTGATCATGGTAAAACTACTCTTACTGATTCATTAA
TTCAAANAGCTGGTATTATTGCANATAAGGTATCTGGTAATGCAGGATATATGTCATGTC
GTCCANATGAACAATTACGTGGNNNNNNNNNN
Length of 5' end seq. 392
3' end seq. ID SHF216Z
3' end seq. 
>SHF216Z.Seq
NNNNNNNNNNTTATCGTCAGACTTAACTGATGATATTGATAATGGTTTGGTGGCTTTTAA
TCAAGATGTTAAAAGTCGTGCCAATTATCTCTATGATAAACATTCTTGGGATAAGAATGA
TGCAGTTAACATTTGGTCATTTGGTCCAGAATATAAGGGTCCAAATGTTTTAGTTAACAA
AACCAAAGGGTGTTCAATATCTTCATGAAATTAAAGACTCTATGGTTTCAGCATTCCAAT
GGATCTCAAATGAAGGTGTATTATGTGGTGAGAAAATGAGATCGATTCGTTTCAACGTTT
ATGATGTAATGCTTCATGCCGATGCAATTCATAGAGGTAGTGGTCAATTTATTCCAACAA
CTCGTCGTGTAATGTATGCCTCCCAACTATCAGCTCAACCAACACTTTTGGAACCAATGT
ATTTGGTTGATATTAGTACTCCTGAAAGTGGTATTAGTGGTATCTACTCTGTACTCAATC
GTAGAAGAGGTGTTGTCATTGGTGAAGAACGTCGTATTGGTACTCCATTATTCAATATCA
AAGCTTATCTTCCTGTAATGGAATCATTTGGTCTCACTGCTGATTTACGTTCAAGTACTG
CTGGTCAAGCTTTCCCTCAATGTGTTTTTGATCATTGGAGTTCAATTGGTATCGTTGGTC
AAGATAAAAGATCAACTGAAGTAGCTTCATCAATTAGAAAGAGAAAAGGTTTAAATCCAA
AATTACCAAC
Length of 3' end seq. 730
Connected seq. ID SHF216P
Connected seq. 
>SHF216P.Seq
GTCATATCAATAATAAATATTATTACATATACAAAACAAAATTAATAAAACTTAAACTTT
ATTTTTCCCCTACATTGGCCAAACTTTAATTTGCCTTTGTACAATTATTCTCTTTAAACT
CTTATTATTAAAATTCATTTTAAAAACTCTCATTTCTAAAAAAAAACTAAAACAAAAAAA
AAANGGTCAATTTTACAATANATCAAATTCGTTCAATTATGGATCGTCGNGAAAATATCA
AAAATATGTCTGTTATTGCTCATGTTGATCATGGTAAAACTACTCTTACTGATTCATTAA
TTCAAANAGCTGGTATTATTGCANATAAGGTATCTGGTAATGCAGGATATATGTCATGTC
GTCCANATGAACAATTACGTGG----------TTATCGTCAGACTTAACTGATGATATTG
ATAATGGTTTGGTGGCTTTTAATCAAGATGTTAAAAGTCGTGCCAATTATCTCTATGATA
AACATTCTTGGGATAAGAATGATGCAGTTAACATTTGGTCATTTGGTCCAGAATATAAGG
GTCCAAATGTTTTAGTTAACAAAACCAAAGGGTGTTCAATATCTTCATGAAATTAAAGAC
TCTATGGTTTCAGCATTCCAATGGATCTCAAATGAAGGTGTATTATGTGGTGAGAAAATG
AGATCGATTCGTTTCAACGTTTATGATGTAATGCTTCATGCCGATGCAATTCATAGAGGT
AGTGGTCAATTTATTCCAACAACTCGTCGTGTAATGTATGCCTCCCAACTATCAGCTCAA
CCAACACTTTTGGAACCAATGTATTTGGTTGATATTAGTACTCCTGAAAGTGGTATTAGT
GGTATCTACTCTGTACTCAATCGTAGAAGAGGTGTTGTCATTGGTGAAGAACGTCGTATT
GGTACTCCATTATTCAATATCAAAGCTTATCTTCCTGTAATGGAATCATTTGGTCTCACT
GCTGATTTACGTTCAAGTACTGCTGGTCAAGCTTTCCCTCAATGTGTTTTTGATCATTGG
AGTTCAATTGGTATCGTTGGTCAAGATAAAAGATCAACTGAAGTAGCTTCATCAATTAGA
AAGAGAAAAGGTTTAAATCCAAAATTACCAAC
Length of connected seq. 1102
Full length Seq ID -
Full length Seq. -
Length of full length seq. -