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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHF211
Library SH
(Link to library)
Clone ID SHF211
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11926-1
Original site URL
Representative seq. ID SHF211P
(Link to Original site)
Representative DNA sequence 
>SHF211 (SHF211Q) /CSM/SH/SHF2-A/SHF211Q.Seq.d/
TAAATGTACTGTAGATTCATGTTCAAACTCAACTGGTTGTGTAAATACTCCAATTAACTG
TGACGATAGTAATCCATGTACCGTAGACTCATGCAATAATTCAACTGGTTGTGTAAACAC
TCCAGTCAATGTTGATGATAATAATCCATGTACCGTCGATGCCTGTACCAAATCAACAGG
TGTTACTCATACCCCAGTCAATGTAGATGATAACAACAAATGTACAATTGATGCATGTAC
CAAAGAAGGTGGTGTAACTCATACCCCAGTCAATACTGATGATAACAATGCCTGTACAAT
TGATGCATGTACAAAAGAAGGTGGTGTAACTCATACTCCAGTCAATACTGATGATAACAA
TGCTTGTACCCTTGACTCCTGTTCACCATCAACTGGCGTTTCCCACACCCCAATTAACTG
TGATGATAGTAATCCATGTACCGTAGACTCATGTTCAAATTCAACCGGTTGTTGCAACAC
TCCAATCAATGTTGATGATAATAATCCATGTACTGTAGATTCATGTACCAAACCAACAGG
TGTCACTCATACCCCAGTCAATGTAGATGATAACAACAAATGTACAATTGATGCATGTAC
CAAAGAAGGTGGTGTAACTCATACTCCAGTCAATACTGATGATAACAATGCATGTACCCT
TGATTCCTGTTCACCATCAACTGGTATTTCCCACGCTCCAATTAACTGTGATGATAGTAA
TCCATGTACCATAGATTCATGCAATAATTCAACTGGGTGTTGCAACACTCCAATCAATGG
TGGATGATAATAATCCATGTNCCGTAAATCATGTACTAAATCAXXXXXXXXXXTNTGGTT
TAANTGGTGGTTTCANTGGTGGTGGNACAGGAGGTAAAGGTGATTGCAAAACTTGTAAAA
ATTAAATAACTCTTTTTANTTAATTATATTTTAAAAAAATTCAAAAAAAATTCNAAAAAA
AATTCAAGAAAAAAAAAAACTATTTGTTTTCTTATCTAATATTTATAATTTTTAAAAATA
AAAATT
sequence update 2002.10.25
Translated Amino Acid sequence 
nvl*ihvqtqlvv*ilqltvtivihvp*thaiiqlvv*tlqsmlmiiihvpsmpvpnqqv
llipqsm*mittnvqlmhvpkkvv*lipqsilmitmpvqlmhvqkkvv*lilqsilmitm
lvpltpvhhqlafptpqltvmivihvp*thvqiqpvvatlqsmlmiiihvl*ihvpnqqv
slipqsm*mittnvqlmhvpkkvv*lilqsilmitmhvplipvhhqlvfptlqltvmivi
hvp*IHAIIQLGVATLQSMVDDNNPCXVNHVLN---

---XGLXGGFXGGGTGGKGDCKTCKN*itlfx*lyfkkiqkkfxkkfkkkknylfsylif
iifknkn
Translated Amino Acid sequence (All Frames) 
Frame A:
*mycrfmfklnwlckysn*l*r**smyrrlmq*fnwlckhssqc****smyrrclyqinr
cysypsqcr**qqmyn*cmyqrrwcnsypsqy***qclyn*cmykrrwcnsyssqy***q
clyp*llftinwrfphpn*l****smyrrlmfkfnrllqhsnqc****smycrfmyqtnr
chsypsqcr**qqmyn*cmyqrrwcnsyssqy***qcmyp*flftinwyfprsn*l****
smyhrfmq*fnwvlqhsnqwwmiiihvp*imy*i---

---XGLXGGFXGGGTGGKGDCKTCKN*itlfx*lyfkkiqkkfxkkfkkkknylfsylif
iifknkn

Frame B:
kctvdscsnstgcvntpincddsnpctvdscnnstgcvntpvnvddnnpctvdactkstg
vthtpvnvddnnkctidactkeggvthtpvntddnnactidactkeggvthtpvntddnn
actldscspstgvshtpincddsnpctvdscsnstgccntpinvddnnpctvdsctkptg
vthtpvnvddnnkctidactkeggvthtpvntddnnactldscspstgishapincddsn
pctidscnnstgccntpingg***smxrksctks---

---xv*xvvsxvvxqevkviaklvkik*lflxnyilkkfkknskknsrkkkticfli*yl
*flkiki

Frame C:
nvl*ihvqtqlvv*ilqltvtivihvp*thaiiqlvv*tlqsmlmiiihvpsmpvpnqqv
llipqsm*mittnvqlmhvpkkvv*lipqsilmitmpvqlmhvqkkvv*lilqsilmitm
lvpltpvhhqlafptpqltvmivihvp*thvqiqpvvatlqsmlmiiihvl*ihvpnqqv
slipqsm*mittnvqlmhvpkkvv*lilqsilmitmhvplipvhhqlvfptlqltvmivi
hvp*IHAIIQLGVATLQSMVDDNNPCXVNHVLN---

---wfxwwfxwwwxrr*r*lqnl*klnnsfxliif*knskkixkkiqekkklfvflsniy
nf*k*k
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHF211 (SHF211Q) /CSM/SH/SHF2-A/SHF211Q.Seq.d/                       1752   0.0
CHI784 (CHI784Q) /CSM/CH/CHI7-D/CHI784Q.Seq.d/                       1534   0.0
SHG420 (SHG420Q) /CSM/SH/SHG4-A/SHG420Q.Seq.d/                       1497   0.0
CHE515 (CHE515Q) /CSM/CH/CHE5-A/CHE515Q.Seq.d/                       1493   0.0
CHF469 (CHF469Q) /CSM/CH/CHF4-C/CHF469Q.Seq.d/                       1362   0.0
CHQ421 (CHQ421Q) /CSM/CH/CHQ4-A/CHQ421Q.Seq.d/                       1324   0.0
SHG235 (SHG235Q) /CSM/SH/SHG2-B/SHG235Q.Seq.d/                       1289   0.0
CHR337 (CHR337Q) /CSM/CH/CHR3-B/CHR337Q.Seq.d/                       1273   0.0
SHF749 (SHF749Q) /CSM/SH/SHF7-C/SHF749Q.Seq.d/                       1267   0.0
CHQ176 (CHQ176Q) /CSM/CH/CHQ1-D/CHQ176Q.Seq.d/                       1245   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X78948|X78948.1 D.minutum ecmB gene.                               50   4e-19  6
CK991559|CK991559.1 EST0111 Eyestalk cDNA library Penaeus monodon cDNA clone ES-N-S03-555-W 5' similar to Histidine-rich glycoprotein precursor (Plasmodium lophurae), mRNA sequence.    30   0.068  4
AC091167|AC091167.19 Homo sapiens chromosome 15 clone RP11-697E2 map 15, *** SEQUENCING IN PROGRESS ***, 2 ordered pieces.    50   0.10   1
AL805962|AL805962.5 Mouse DNA sequence from clone RP23-121I18 on chromosome X.    50   0.10   1
AC133847|AC133847.3 Rattus norvegicus clone CH230-7I2, WORKING DRAFT SEQUENCE, 2 unordered pieces.    48   0.40   1
AC130411|AC130411.1 Homo sapiens chromosome 3 clone RP11-4M21, *** SEQUENCING IN PROGRESS ***, 6 unordered pieces.    48   0.40   1
AC127742|AC127742.3 Rattus norvegicus clone CH230-175K23, *** SEQUENCING IN PROGRESS ***, 6 unordered pieces.    48   0.40   1
AC120676|AC120676.4 Rattus norvegicus clone CH230-38D7, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    48   0.40   1
AC012584|AC012584.6 Homo sapiens clone RP11-4M21, WORKING DRAFT SEQUENCE, 14 unordered pieces.    48   0.40   1
AC106287|AC106287.4 Rattus norvegicus clone CH230-68I11, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    48   0.40   1
dna update 2006. 3.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A27020(A27020)DIF-induced prestalk pDd63 protein precursor - sli...   416   e-115
(P11976) RecName: Full=Prestalk protein; AltName: Full=Extracell...   402   e-111
A26838(A26838)prestalk protein precursor - slime mold (Dictyoste...   402   e-111
S44208(S44208) extracellular matrix protein B - Dictyostelium mi...   331   2e-89
AC116984_103(AC116984|pid:none) Dictyostelium discoideum chromos...   315   2e-84
AC117072_61(AC117072|pid:none) Dictyostelium discoideum chromoso...   294   3e-78
(Q54C31) RecName: Full=Protein psiR; Flags: Precursor;                138   3e-31
(Q54G85) RecName: Full=Protein psiJ; Flags: Precursor;                137   4e-31
(Q54CH8) RecName: Full=Protein psiN; Flags: Precursor;                134   4e-30
(Q557E9) RecName: Full=Protein psiG; Flags: Precursor;  &AC11568...   117   5e-25
protein update 2009. 4.18
PSORT 

 psg: 0.88  gvh: 0.51  alm: 0.42  top: 0.33  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 24.0 %: nuclear
	 16.0 %: mitochondrial
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal

>> prediction for SHF211 is cyt
5' end seq. ID SHF211F
5' end seq. 
>SHF211F.Seq
TAAATGTACTGTAGATTCATGTTCAAACTCAACTGGTTGTGTAAATACTCCAATTAACTG
TGACGATAGTAATCCATGTACCGTAGACTCATGCAATAATTCAACTGGTTGTGTAAACAC
TCCAGTCAATGTTGATGATAATAATCCATGTACCGTCGATGCCTGTACCAAATCAACAGG
TGTTACTCATACCCCAGTCAATGTAGATGATAACAACAAATGTACAATTGATGCATGTAC
CAAAGAAGGTGGTGTAACTCATACCCCAGTCAATACTGATGATAACAATGCCTGTACAAT
TGATGCATGTACAAAAGAAGGTGGTGTAACTCATACTCCAGTCAATACTGATGATAACAA
TGCTTGTACCCTTGACTCCTGTTCACCATCAACTGGCGTTTCCCACACCCCAATTAACTG
TGATGATAGTAATCCATGTACCGTAGACTCATGTTCAAATTCAACCGGTTGTTGCAACAC
TCCAATCAATGTTGATGATAATAATCCATGTACTGTAGATTCATGTACCAAACCAACAGG
TGTCACTCATACCCCAGTCAATGTAGATGATAACAACAAATGTACAATTGATGCATGTAC
CAAAGAAGGTGGTGTAACTCATACTCCAGTCAATACTGATGATAACAATGCATGTACCCT
TGATTCCTGTTCACCATCAACTGGTATTTCCCACGCTCCAATTAACTGTGATGATAGTAA
TCCATGTACCATAGATTCATGCAATAATTCAACTGGGTGTTGCAACACTCCAATCAATGG
TGGATGATAATAATCCATGTNCCGTAAATCATGTACTAAATCANNNNNNNNNN
Length of 5' end seq. 833
3' end seq. ID SHF211Z
3' end seq. 
>SHF211Z.Seq
NNNNNNNNNNTNTGGTTTAANTGGTGGTTTCANTGGTGGTGGNACAGGAGGTAAAGGTGA
TTGCAAAACTTGTAAAAATTAAATAACTCTTTTTANTTAATTATATTTTAAAAAAATTCA
AAAAAAATTCNAAAAAAAATTCAAGAAAAAAAAAAACTATTTGTTTTCTTATCTAATATT
TATAATTTTTAAAAATAAAAATT
Length of 3' end seq. 203
Connected seq. ID SHF211P
Connected seq. 
>SHF211P.Seq
TAAATGTACTGTAGATTCATGTTCAAACTCAACTGGTTGTGTAAATACTCCAATTAACTG
TGACGATAGTAATCCATGTACCGTAGACTCATGCAATAATTCAACTGGTTGTGTAAACAC
TCCAGTCAATGTTGATGATAATAATCCATGTACCGTCGATGCCTGTACCAAATCAACAGG
TGTTACTCATACCCCAGTCAATGTAGATGATAACAACAAATGTACAATTGATGCATGTAC
CAAAGAAGGTGGTGTAACTCATACCCCAGTCAATACTGATGATAACAATGCCTGTACAAT
TGATGCATGTACAAAAGAAGGTGGTGTAACTCATACTCCAGTCAATACTGATGATAACAA
TGCTTGTACCCTTGACTCCTGTTCACCATCAACTGGCGTTTCCCACACCCCAATTAACTG
TGATGATAGTAATCCATGTACCGTAGACTCATGTTCAAATTCAACCGGTTGTTGCAACAC
TCCAATCAATGTTGATGATAATAATCCATGTACTGTAGATTCATGTACCAAACCAACAGG
TGTCACTCATACCCCAGTCAATGTAGATGATAACAACAAATGTACAATTGATGCATGTAC
CAAAGAAGGTGGTGTAACTCATACTCCAGTCAATACTGATGATAACAATGCATGTACCCT
TGATTCCTGTTCACCATCAACTGGTATTTCCCACGCTCCAATTAACTGTGATGATAGTAA
TCCATGTACCATAGATTCATGCAATAATTCAACTGGGTGTTGCAACACTCCAATCAATGG
TGGATGATAATAATCCATGTNCCGTAAATCATGTACTAAATCA----------TNTGGTT
TAANTGGTGGTTTCANTGGTGGTGGNACAGGAGGTAAAGGTGATTGCAAAACTTGTAAAA
ATTAAATAACTCTTTTTANTTAATTATATTTTAAAAAAATTCAAAAAAAATTCNAAAAAA
AATTCAAGAAAAAAAAAAACTATTTGTTTTCTTATCTAATATTTATAATTTTTAAAAATA
AAAATT
Length of connected seq. 1016
Full length Seq ID -
Full length Seq. -
Length of full length seq. -