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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHF181
Library SH
(Link to library)
Clone ID SHF181
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11865-1
Original site URL
Representative seq. ID SHF181P
(Link to Original site)
Representative DNA sequence 
>SHF181 (SHF181Q) /CSM/SH/SHF1-D/SHF181Q.Seq.d/
ACTGGAATGTCATTATCATCAAATTCAATGGCAATATTTTCAGCTGTAGATAATCCAAAT
TCACCAAATCAAAATAATCAAAATCCATATGATACTGAATCGATTATTTCTGGTTATGAT
AGAGATGATTTATCGGAGTACGATTCAGAGAATGAAGAATCTAGACAGCAAACAATTCAT
AGATCAACATCCACTTTCTCAATTCAATCACCGTCTCGTGGTGATAGGGTTTTAAATAGA
GCTGAAAGTAATTTTATAACAAGTGTAAATAGTAGTAATAATAAACAAACTTCTCAACCA
TTAAGAGATGATATAAAATCAAACAGTAGTGATAAACTATTACAACAACCAGATAATATT
GATCCATATGAAATTCAACAAAATAAACAATCCAATAATAGCAATAGCAATAGCAATAGA
AATTTAACACCAAATTCATCATCACCAACAAATCAAAGAAAAAATAAACAAGAAGATGAT
GATGATGAATCTAAATTAGATGATGAATCAGATTTGAATGAAAGATTTCAAAAAGTTGTA
CTTAGATTTAGAGAATTTCCATCATTGGATACAAATCTTTATCGCTTACAAGAGATTTGT
ACAGATTTATTACATATTTCTCAAGATTTTATACATACTGTCXXXXXXXXXXTTATGATC
CAGTGATTGGTCGTCATTTATTGTTCACTAGATTCAGAGATATGACTCATTTGAAATTTA
GAAAGAAAACCATTGAAAAAGTGGCCGATACTCAATCAAATCTTTGGAATCATTATCAAC
CATTCGATACTAGTGATTTGAAAAAGATTGGTGTACGTGTTAAACATACTGATTTGGTTA
CAAATGCTGAAGGTACTTTCTTTTATGCTAAAGCAATGACTGAACGTTCAATTGGTACCG
CCATTCATTTATTACATAAAGCTAGAAAATTCTTTGAATTGGCATTAATTTCAAATCCAA
ACAATAAGGAAACNTTACAACTATGTGCTCAAGCTTGGTGTAAAATTTTGGAATTTAGTG
CAAGTCAAGGTAAAAATATGTCCAATGTTAGATTTTCAATGTCTGATCGTGTCGTGGTAA
ATACTGATCGTTATTTCTTACGTGCTATCGATGCCGATACTAAAGGTCCCCTCATTNTCT
TCTTTTATGCAAACTTTTTAGTTCGTTGTGAACGTTATGAAAAAGCTGAAGATTATTTCC
TTCGTTCTCTTGAAGCTGATTCAAATAATTATCGTTGTTTAACCGCTTATGCAAACTTTT
TAATTGAAAGAGGTTTTCCAAATGAATCTTCTCTTTTCTTAGAAAGAGCAAAACAATGTA
AAGCAATCTTAGTTGGTGGTTTTAAAAATTAAATTTTTAAAAAGATTTAACATATATATA
ATATTAAATAAATAAATTAAATTAAATAAATAAATATAAAAATATAAAAATA
sequence update 2002.10.25
Translated Amino Acid sequence 
TGMSLSSNSMAIFSAVDNPNSPNQNNQNPYDTESIISGYDRDDLSEYDSENEESRQQTIH
RSTSTFSIQSPSRGDRVLNRAESNFITSVNSSNNKQTSQPLRDDIKSNSSDKLLQQPDNI
DPYEIQQNKQSNNSNSNSNRNLTPNSSSPTNQRKNKQEDDDDESKLDDESDLNERFQKVV
LRFREFPSLDTNLYRLQEICTDLLHISQDFIHTV---

---YDPVIGRHLLFTRFRDMTHLKFRKKTIEKVADTQSNLWNHYQPFDTSDLKKIGVRVK
HTDLVTNAEGTFFYAKAMTERSIGTAIHLLHKARKFFELALISNPNNKETLQLCAQAWCK
ILEFSASQGKNMSNVRFSMSDRVVVNTDRYFLRAIDADTKGPLIXFFYANFLVRCERYEK
AEDYFLRSLEADSNNYRCLTAYANFLIERGFPNESSLFLERAKQCKAILVGGFKN*ifkk
i*hiynik*in*ik*iniki*k
Translated Amino Acid sequence (All Frames) 
Frame A:
TGMSLSSNSMAIFSAVDNPNSPNQNNQNPYDTESIISGYDRDDLSEYDSENEESRQQTIH
RSTSTFSIQSPSRGDRVLNRAESNFITSVNSSNNKQTSQPLRDDIKSNSSDKLLQQPDNI
DPYEIQQNKQSNNSNSNSNRNLTPNSSSPTNQRKNKQEDDDDESKLDDESDLNERFQKVV
LRFREFPSLDTNLYRLQEICTDLLHISQDFIHTV---

---l*ssdwssfivh*iqrydsfei*kenh*ksgrysiksleslstiry**fekdwctc*
ty*fgykc*ryfllc*snd*tfnwyrhsfit*s*kil*iginfkskq*gnxttmcsslv*
nfgi*cksr*kyvqc*ifnv*scrgky*slfltcyrcry*rsphxlllcklfssl*tl*k
s*rlfpsfs*s*fk*lslfnrlcklfn*krfsk*ifsflrksktm*snlswwf*klnf*k
dltyi*y*inkln*inkykniki

Frame B:
lechyhqiqwqyfql*iiqihqikiikihmilnrlflvmiemiyrstiqrmknldskqfi
dqhplsqfnhrlvvigf*ielkvil*qv*ivviinkllnh*emi*nqtvvinyynnqiil
ihmkfnkinnpiiaiaiaiei*hqihhhqqikekinkkmmmmnln*mmnqi*mkdfkkly
ldlenfhhwiqifiaykrfvqiyyiflkilyil---

---YDPVIGRHLLFTRFRDMTHLKFRKKTIEKVADTQSNLWNHYQPFDTSDLKKIGVRVK
HTDLVTNAEGTFFYAKAMTERSIGTAIHLLHKARKFFELALISNPNNKETLQLCAQAWCK
ILEFSASQGKNMSNVRFSMSDRVVVNTDRYFLRAIDADTKGPLIXFFYANFLVRCERYEK
AEDYFLRSLEADSNNYRCLTAYANFLIERGFPNESSLFLERAKQCKAILVGGFKN*ifkk
i*hiynik*in*ik*iniki*k

Frame C:
wnviiikfngnifscr*skftksk*sksi*y*idyfwl**r*figvrfre*ri*tanns*
inihflnsitvsw**gfk*s*k*fynkck*****tnfstikr*ykikq***titttr*y*
si*nstk*tiq**q*q*q*kfntkfiitnkskkk*trr****i*ir**irfe*kisksct
*i*risiigykslsltrdlyrfityfsrfytyc---

---miq*lvviycsldsei*li*nlerkplkkwpilnqifgiiinhsilvi*krlvyvln
iliwlqmlkvlsfmlkq*lnvqlvppfiyyiklenslnwh*fqiqtirkxynyvlklgvk
fwnlvqvkvkicpmldfqclivsw*ilivisyvlsmpilkvpsxssfmqtf*fvvnvmkk
lkiisfvllkliqiiivv*plmqtf*lkevfqmnllfs*keqnnvkqs*lvvlkikflkr
fniyiilnk*iklnk*i*kykn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHF181 (SHF181Q) /CSM/SH/SHF1-D/SHF181Q.Seq.d/                       2593   0.0
SHD552 (SHD552Q) /CSM/SH/SHD5-C/SHD552Q.Seq.d/                       1344   0.0
SHH870 (SHH870Q) /CSM/SH/SHH8-C/SHH870Q.Seq.d/                       1219   0.0
CHR591 (CHR591Q) /CSM/CH/CHR5-D/CHR591Q.Seq.d/                       1207   0.0
CHN252 (CHN252Q) /CSM/CH/CHN2-C/CHN252Q.Seq.d/                       1160   0.0
VSC329 (VSC329Q) /CSM/VS/VSC3-B/VSC329Q.Seq.d/                         54   6e-06
CFI409 (CFI409Q) /CSM/CF/CFI4-A/CFI409Q.Seq.d/                         50   1e-04
AHF808 (AHF808Q) /CSM/AH/AHF8-A/AHF808Q.Seq.d/                         48   4e-04
VSC328 (VSC328Q) /CSM/VS/VSC3-B/VSC328Q.Seq.d/                         46   0.001
SSD825 (SSD825Q) /CSM/SS/SSD8-B/SSD825Q.Seq.d/                         44   0.006
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AY220914|AY220914.1 Dictyostelium discoideum ComB (comB) gene, complete cds.  1352   0.0    1
U66912|U66912.1 Dictyostelium discoideum ORF DG1016 gene, partial cds.   319   1e-82  1
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    48   1e-05  15
AC116918|AC116918.3 Dictyostelium discoideum chromosome 2 map 6143698-6163521 strain AX4, complete sequence.    50   1e-04  3
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    46   2e-04  16
AC117072|AC117072.3 Dictyostelium discoideum chromosome 2 map 3879572-4071762 strain AX4, complete sequence.    42   4e-04  8
CW344417|CW344417.1 104_847_11487943_148_36198_030 Sorghum methylation filtered library (LibID: 104) Sorghum bicolor genomic clone 11487943, genomic survey sequence.    48   0.003  2
CW344416|CW344416.1 104_847_11487943_116_36197_030 Sorghum methylation filtered library (LibID: 104) Sorghum bicolor genomic clone 11487943, genomic survey sequence.    48   0.003  2
AY232271|AY232271.1 Dictyostelium discoideum putative protein Roco9 (roco9) gene, complete cds.    34   0.004  6
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    38   0.005  13
dna update 2006. 3.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY220914_1(AY220914|pid:none) Dictyostelium discoideum ComB (com...   466   e-130
AC116963_55(AC116963|pid:none) Dictyostelium discoideum chromoso...    96   3e-18
U66912_1(U66912|pid:none) Dictyostelium discoideum ORF DG1016 ge...    82   3e-14
AP009380_1340(AP009380|pid:none) Porphyromonas gingivalis ATCC 3...    49   6e-04
CP000099_2445(CP000099|pid:none) Methanosarcina barkeri str. Fus...    42   0.068
CP000386_3087(CP000386|pid:none) Rubrobacter xylanophilus DSM 99...    41   0.12
CP000099_2442(CP000099|pid:none) Methanosarcina barkeri str. Fus...    40   0.26
BT052510_1(BT052510|pid:none) Medicago truncatula clone MTYFD_FE...    40   0.26
AE016819_325(AE016819|pid:none) Ashbya gossypii (= Eremothecium ...    39   0.44
CP000817_2823(CP000817|pid:none) Lysinibacillus sphaericus C3-41...    39   0.57
protein update 2009. 4.18
PSORT 

 psg: 0.93  gvh: 0.46  alm: 0.48  top: 0.33  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 20.0 %: nuclear
	 16.0 %: extracellular, including cell wall
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for SHF181 is cyt
5' end seq. ID SHF181F
5' end seq. 
>SHF181F.Seq
ACTGGAATGTCATTATCATCAAATTCAATGGCAATATTTTCAGCTGTAGATAATCCAAAT
TCACCAAATCAAAATAATCAAAATCCATATGATACTGAATCGATTATTTCTGGTTATGAT
AGAGATGATTTATCGGAGTACGATTCAGAGAATGAAGAATCTAGACAGCAAACAATTCAT
AGATCAACATCCACTTTCTCAATTCAATCACCGTCTCGTGGTGATAGGGTTTTAAATAGA
GCTGAAAGTAATTTTATAACAAGTGTAAATAGTAGTAATAATAAACAAACTTCTCAACCA
TTAAGAGATGATATAAAATCAAACAGTAGTGATAAACTATTACAACAACCAGATAATATT
GATCCATATGAAATTCAACAAAATAAACAATCCAATAATAGCAATAGCAATAGCAATAGA
AATTTAACACCAAATTCATCATCACCAACAAATCAAAGAAAAAATAAACAAGAAGATGAT
GATGATGAATCTAAATTAGATGATGAATCAGATTTGAATGAAAGATTTCAAAAAGTTGTA
CTTAGATTTAGAGAATTTCCATCATTGGATACAAATCTTTATCGCTTACAAGAGATTTGT
ACAGATTTATTACATATTTCTCAAGATTTTATACATACTGTCNNNNNNNNNN
Length of 5' end seq. 652
3' end seq. ID SHF181Z
3' end seq. 
>SHF181Z.Seq
NNNNNNNNNNTTATGATCCAGTGATTGGTCGTCATTTATTGTTCACTAGATTCAGAGATA
TGACTCATTTGAAATTTAGAAAGAAAACCATTGAAAAAGTGGCCGATACTCAATCAAATC
TTTGGAATCATTATCAACCATTCGATACTAGTGATTTGAAAAAGATTGGTGTACGTGTTA
AACATACTGATTTGGTTACAAATGCTGAAGGTACTTTCTTTTATGCTAAAGCAATGACTG
AACGTTCAATTGGTACCGCCATTCATTTATTACATAAAGCTAGAAAATTCTTTGAATTGG
CATTAATTTCAAATCCAAACAATAAGGAAACNTTACAACTATGTGCTCAAGCTTGGTGTA
AAATTTTGGAATTTAGTGCAAGTCAAGGTAAAAATATGTCCAATGTTAGATTTTCAATGT
CTGATCGTGTCGTGGTAAATACTGATCGTTATTTCTTACGTGCTATCGATGCCGATACTA
AAGGTCCCCTCATTNTCTTCTTTTATGCAAACTTTTTAGTTCGTTGTGAACGTTATGAAA
AAGCTGAAGATTATTTCCTTCGTTCTCTTGAAGCTGATTCAAATAATTATCGTTGTTTAA
CCGCTTATGCAAACTTTTTAATTGAAAGAGGTTTTCCAAATGAATCTTCTCTTTTCTTAG
AAAGAGCAAAACAATGTAAAGCAATCTTAGTTGGTGGTTTTAAAAATTAAATTTTTAAAA
AGATTTAACATATATATAATATTAAATAAATAAATTAAATTAAATAAATAAATATAAAAA
TATAAAAATA
Length of 3' end seq. 790
Connected seq. ID SHF181P
Connected seq. 
>SHF181P.Seq
ACTGGAATGTCATTATCATCAAATTCAATGGCAATATTTTCAGCTGTAGATAATCCAAAT
TCACCAAATCAAAATAATCAAAATCCATATGATACTGAATCGATTATTTCTGGTTATGAT
AGAGATGATTTATCGGAGTACGATTCAGAGAATGAAGAATCTAGACAGCAAACAATTCAT
AGATCAACATCCACTTTCTCAATTCAATCACCGTCTCGTGGTGATAGGGTTTTAAATAGA
GCTGAAAGTAATTTTATAACAAGTGTAAATAGTAGTAATAATAAACAAACTTCTCAACCA
TTAAGAGATGATATAAAATCAAACAGTAGTGATAAACTATTACAACAACCAGATAATATT
GATCCATATGAAATTCAACAAAATAAACAATCCAATAATAGCAATAGCAATAGCAATAGA
AATTTAACACCAAATTCATCATCACCAACAAATCAAAGAAAAAATAAACAAGAAGATGAT
GATGATGAATCTAAATTAGATGATGAATCAGATTTGAATGAAAGATTTCAAAAAGTTGTA
CTTAGATTTAGAGAATTTCCATCATTGGATACAAATCTTTATCGCTTACAAGAGATTTGT
ACAGATTTATTACATATTTCTCAAGATTTTATACATACTGTC----------TTATGATC
CAGTGATTGGTCGTCATTTATTGTTCACTAGATTCAGAGATATGACTCATTTGAAATTTA
GAAAGAAAACCATTGAAAAAGTGGCCGATACTCAATCAAATCTTTGGAATCATTATCAAC
CATTCGATACTAGTGATTTGAAAAAGATTGGTGTACGTGTTAAACATACTGATTTGGTTA
CAAATGCTGAAGGTACTTTCTTTTATGCTAAAGCAATGACTGAACGTTCAATTGGTACCG
CCATTCATTTATTACATAAAGCTAGAAAATTCTTTGAATTGGCATTAATTTCAAATCCAA
ACAATAAGGAAACNTTACAACTATGTGCTCAAGCTTGGTGTAAAATTTTGGAATTTAGTG
CAAGTCAAGGTAAAAATATGTCCAATGTTAGATTTTCAATGTCTGATCGTGTCGTGGTAA
ATACTGATCGTTATTTCTTACGTGCTATCGATGCCGATACTAAAGGTCCCCTCATTNTCT
TCTTTTATGCAAACTTTTTAGTTCGTTGTGAACGTTATGAAAAAGCTGAAGATTATTTCC
TTCGTTCTCTTGAAGCTGATTCAAATAATTATCGTTGTTTAACCGCTTATGCAAACTTTT
TAATTGAAAGAGGTTTTCCAAATGAATCTTCTCTTTTCTTAGAAAGAGCAAAACAATGTA
AAGCAATCTTAGTTGGTGGTTTTAAAAATTAAATTTTTAAAAAGATTTAACATATATATA
ATATTAAATAAATAAATTAAATTAAATAAATAAATATAAAAATATAAAAATA
Length of connected seq. 1422
Full length Seq ID -
Full length Seq. -
Length of full length seq. -