>SHF180 (SHF180Q) /CSM/SH/SHF1-D/SHF180Q.Seq.d/
ATTTACAATTTGTTTATAATTACAAATAACTATTTTTATATTTTTATATTTATTATTTTT
TTTAATATATTTTTTTATTTTCATTTTTTTGTATAAACTTACATAAAAAGATAAAAAGAA
AAAAATGTCTAAACCAATGGATGTAGAACAAGAACCAAAAATTGATGATGCTTTATATAG
CAGACAACTTTATGCATTATCTCATGAAACAATGAAAAAAATTACATCAACTTCAGTTTT
AGTTGTAGGTTTACAAGGTCTTGGTATTGAAATTGTTAAAGATTTAAGTTTAGCAGGTGT
TAAATCTGTTACATTGTATGATAAAGAATTAGTTGAAATTAAAGATTTGTCTTCTCAATT
TTATTTTTCACCAGAACAAGTTGGTAAAGTTGGTAGAGCAGATGCATGTTTCCAAAAAGT
TGTAGATTTAAATAATTATGTTAGAATTGATGTACATAATGGAGAATTAAGTGATGAATT
CTTAAAGAAATTCAATGTTGTAGTATTAGCCAATCAACCATTAGCATTACAACTCAAAGT
GAATGAATTTTGTCATGCAAATXXXXXXXXXXACACTCAACAAGCTGGTGGTGATGCTGA
AGATGATCAATGTGATACCATTTTATCACAACTTCCNCCAACCAAGTGAAATGGCTGGTT
ACAAAATTAACTCTATTCAATTTGAAAAGGATGATGATACCAATCATCATATCGATTTCA
TTACCGCTACCTCAAATTTAAGAGCTACCAACTATGCAATCAGTCCAGCTGATAAACATA
AGACCAAAGGTATCGCCGGTAAAATCATTCCAGCTTTAGTTACCACTACTGCCGTAGTTG
CTGGTTTCGTTTGTATCGAATTGATTAAAGTTATTCAAAATAAAGCTTTGGAGAAATATA
AGAGCACCTTTATGAATTTAGGTATCCCATTCTTTGGTTTCGTTGAACCAATTGCCGCCC
CAAAGAACAAAATTCGTGAAGGCTGGACTTGGACACTTTGGGATCGTTTCGATGTTGATG
GTGATATCACTCTCAAGGAGTTTTTAGATCTCTTTGAAAAGAAACATGGTTTGGATATTT
CAATGCTCTCTTGTAAAGTTACACTTTTATATGCTCTCTTCACTGATAAGAAAACTAAAG
AGGAAAGATTAAAGATGAAAATCTCACAACTCTATGAAACCCTCTCAAAGAAACCATTAC
CAAAAGATAAGAAATATTTATTACTNAAATTTGTTGCAA

fticl*lqitififlyllffliyffififlyklt*KDKKKKMSKPMDVEQEPKIDDALYS
RQLYALSHETMKKITSTSVLVVGLQGLGIEIVKDLSLAGVKSVTLYDKELVEIKDLSSQF
YFSPEQVGKVGRADACFQKVVDLNNYVRIDVHNGELSDEFLKKFNVVVLANQPLALQLKV
NEFCHAN---

---TLNKLVVMLKMINVIPFYHNFXQPSEMAGYKINSIQFEKDDDTNHHIDFITATSNLR
ATNYAISPADKHKTKGIAGKIIPALVTTTAVVAGFVCIELIKVIQNKALEKYKSTFMNLG
IPFFGFVEPIAAPKNKIREGWTWTLWDRFDVDGDITLKEFLDLFEKKHGLDISMLSCKVT
LLYALFTDKKTKEERLKMKISQLYETLSKKPLPKDKKYLLLKFVA

Frame A:
iynlfiitnnyfyififiiffniffyfhffv*tyikr*keknv*tngcrtrtkn**cfi*
qttlciis*nneknyinfsfscrftrswy*nc*rfkfsrc*icyiv**ris*n*rfvfsi
lfftrtsw*sw*srcmfpkscrfk*lc*n*ct*wrik**ilkeiqccsisqstisittqs
e*ilsck---

---TLNKLVVMLKMINVIPFYHNFXQPSEMAGYKINSIQFEKDDDTNHHIDFITATSNLR
ATNYAISPADKHKTKGIAGKIIPALVTTTAVVAGFVCIELIKVIQNKALEKYKSTFMNLG
IPFFGFVEPIAAPKNKIREGWTWTLWDRFDVDGDITLKEFLDLFEKKHGLDISMLSCKVT
LLYALFTDKKTKEERLKMKISQLYETLSKKPLPKDKKYLLLKFVA

Frame B:
fticl*lqitififlyllffliyffififlyklt*KDKKKKMSKPMDVEQEPKIDDALYS
RQLYALSHETMKKITSTSVLVVGLQGLGIEIVKDLSLAGVKSVTLYDKELVEIKDLSSQF
YFSPEQVGKVGRADACFQKVVDLNNYVRIDVHNGELSDEFLKKFNVVVLANQPLALQLKV
NEFCHAN---

---hstsww*c*r*sm*yhfittsxnqvkwlvtkltlfnlkrmmipiiisislplpqi*e
lptmqsvqlinirpkvspvksfql*lpllp*llvsfvsn*lklfkiklwrnirapl*i*v
shslvslnqlppqrtkfvkaglghfgivsmlmvislsrsf*islkrnmvwifqcslvklh
fymlsslirklkrkd*r*kshnsmkpsqrnhyqkirniyyxnllq

Frame C:
lqfvynyk*lflyfyiyyff*yiflfsffcinlhkkikrkkclnqwm*nknqklmmlyia
dnfmhylmkq*kklhqlqf*l*vykvlvlkllki*v*qvlnllhcmikn*lklkicllnf
ifhqnklvklveqmhvskkl*i*iimlelmyimen*vmns*rnsml*y*pinh*hynsk*
mnfvmq---

---tqqaggdaeddqcdtilsqlpptk*ngwlqn*lysi*kg**yqssyrfhyrylkfks
yqlcnqss**t*dqryrr*nhssfsyhycrscwfrlyrid*sysk*sfgei*ehlyefry
pilwfr*tncrpkeqns*rldldtlgsfrc*w*yhsqgvfrsl*ketwfgyfnall*syt
ficslh**en*rgkikdenlttl*nplketitkr*eifitxicc

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHF180 (SHF180Q) /CSM/SH/SHF1-D/SHF180Q.Seq.d/                       2095   0.0
SHJ593 (SHJ593Q) /CSM/SH/SHJ5-D/SHJ593Q.Seq.d/                       1310   0.0
AHQ282 (AHQ282Q) /CSM/AH/AHQ2-D/AHQ282Q.Seq.d/                       1310   0.0
CHO831 (CHO831Q) /CSM/CH/CHO8-B/CHO831Q.Seq.d/                       1308   0.0
AHK113 (AHK113Q) /CSM/AH/AHK1-A/AHK113Q.Seq.d/                       1296   0.0
VHP810 (VHP810Q) /CSM/VH/VHP8-A/VHP810Q.Seq.d/                       1294   0.0
VHP750 (VHP750Q) /CSM/VH/VHP7-C/VHP750Q.Seq.d/                       1294   0.0
VHP205 (VHP205Q) /CSM/VH/VHP2-A/VHP205Q.Seq.d/                       1294   0.0
VHO562 (VHO562Q) /CSM/VH/VHO5-C/VHO562Q.Seq.d/                       1294   0.0
VHN723 (VHN723Q) /CSM/VH/VHN7-A/VHN723Q.Seq.d/                       1294   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CB933531|CB933531.1 ri31b11.y1 Meloidogyne chitwoodi egg pAMP1 v1 Meloidogyne chitwoodisequence.    64   3e-12  2
AZ930776|AZ930776.1 474.dhz58e03.s1 Saccharomyces unisporus NRRL Y-1556 Saccharomyces unisporus genomic clone 474.dhz58e03.s1, DNA sequence.    68   1e-11  2
DR174755|DR174755.1 HR0003_N01:A Triphysaria versicolor (parasitic plant) root-tip, host reverse SSH library Triphysaria versicolor cDNA, mRNA sequence.    58   5e-04  1
DR174741|DR174741.1 HR0003_M08:A Triphysaria versicolor (parasitic plant) root-tip, host reverse SSH library Triphysaria versicolor cDNA, mRNA sequence.    58   5e-04  1
DR174736|DR174736.1 HR0003_M02:A Triphysaria versicolor (parasitic plant) root-tip, host reverse SSH library Triphysaria versicolor cDNA, mRNA sequence.    58   5e-04  1
AC134210|AC134210.1 Rattus norvegicus clone CH230-197N6, *** SEQUENCING IN PROGRESS ***, 60 unordered pieces.    58   5e-04  1
AC108277|AC108277.6 Rattus norvegicus clone CH230-27E16, *** SEQUENCING IN PROGRESS ***, 8 unordered pieces.    58   5e-04  1
BZ303839|BZ303839.1 KD2618.q1 Kluyveromyces delphensis Random Genomic Library Kluyveromyces delphensis genomic clone KD2618, DNA sequence.    48   0.002  2
DR028930|DR028930.1 bda010063_A05_11.b1_024.ab1 Antrodia cinnamomea cDNA Library Antrodia cinnamomea cDNA, mRNA sequence.    52   0.003  2
BH154952|BH154952.1 ENTTP11TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52   0.011  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55C16) RecName: Full=Ubiquitin-like modifier-activating enzyme...   367   e-107
EF468345_1(EF468345|pid:none) Pisum sativum ubiquitin activating...   223   8e-57
CR382138_755(CR382138|pid:none) Debaryomyces hansenii strain CBS...   207   8e-52
AE017341_129(AE017341|pid:none) Cryptococcus neoformans var. neo...   206   2e-51
CP000496_820(CP000496|pid:none) Pichia stipitis CBS 6054 chromos...   204   6e-51
BX908809_46(BX908809|pid:none) Neurospora crassa DNA linkage gro...   200   9e-50
BC047256_1(BC047256|pid:none) Xenopus laevis ubiquitin activatin...   199   1e-49
AB040073_1(AB040073|pid:none) Xenopus laevis E1 mRNA for ubiquit...   199   1e-49
AB035495_1(AB035495|pid:none) Carassius auratus mRNA for ubiquit...   199   2e-49
(P22515) RecName: Full=Ubiquitin-activating enzyme E1 1;  &S3804...   198   4e-49

 psg: 0.50  gvh: 0.52  alm: 0.27  top: 0.43  tms: 0.07  mit: 0.21  mip: 0.00
 nuc: 0.13  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: cytoskeletal
	 12.0 %: mitochondrial
	  8.0 %: peroxisomal
	  4.0 %: vacuolar
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for SHF180 is cyt

>SHF180F.Seq
ATTTACAATTTGTTTATAATTACAAATAACTATTTTTATATTTTTATATTTATTATTTTT
TTTAATATATTTTTTTATTTTCATTTTTTTGTATAAACTTACATAAAAAGATAAAAAGAA
AAAAATGTCTAAACCAATGGATGTAGAACAAGAACCAAAAATTGATGATGCTTTATATAG
CAGACAACTTTATGCATTATCTCATGAAACAATGAAAAAAATTACATCAACTTCAGTTTT
AGTTGTAGGTTTACAAGGTCTTGGTATTGAAATTGTTAAAGATTTAAGTTTAGCAGGTGT
TAAATCTGTTACATTGTATGATAAAGAATTAGTTGAAATTAAAGATTTGTCTTCTCAATT
TTATTTTTCACCAGAACAAGTTGGTAAAGTTGGTAGAGCAGATGCATGTTTCCAAAAAGT
TGTAGATTTAAATAATTATGTTAGAATTGATGTACATAATGGAGAATTAAGTGATGAATT
CTTAAAGAAATTCAATGTTGTAGTATTAGCCAATCAACCATTAGCATTACAACTCAAAGT
GAATGAATTTTGTCATGCAAATNNNNNNNNNN

>SHF180Z.Seq
NNNNNNNNNNACACTCAACAAGCTGGTGGTGATGCTGAAGATGATCAATGTGATACCATT
TTATCACAACTTCCNCCAACCAAGTGAAATGGCTGGTTACAAAATTAACTCTATTCAATT
TGAAAAGGATGATGATACCAATCATCATATCGATTTCATTACCGCTACCTCAAATTTAAG
AGCTACCAACTATGCAATCAGTCCAGCTGATAAACATAAGACCAAAGGTATCGCCGGTAA
AATCATTCCAGCTTTAGTTACCACTACTGCCGTAGTTGCTGGTTTCGTTTGTATCGAATT
GATTAAAGTTATTCAAAATAAAGCTTTGGAGAAATATAAGAGCACCTTTATGAATTTAGG
TATCCCATTCTTTGGTTTCGTTGAACCAATTGCCGCCCCAAAGAACAAAATTCGTGAAGG
CTGGACTTGGACACTTTGGGATCGTTTCGATGTTGATGGTGATATCACTCTCAAGGAGTT
TTTAGATCTCTTTGAAAAGAAACATGGTTTGGATATTTCAATGCTCTCTTGTAAAGTTAC
ACTTTTATATGCTCTCTTCACTGATAAGAAAACTAAAGAGGAAAGATTAAAGATGAAAAT
CTCACAACTCTATGAAACCCTCTCAAAGAAACCATTACCAAAAGATAAGAAATATTTATT
ACTNAAATTTGTTGCAA

>SHF180P.Seq
ATTTACAATTTGTTTATAATTACAAATAACTATTTTTATATTTTTATATTTATTATTTTT
TTTAATATATTTTTTTATTTTCATTTTTTTGTATAAACTTACATAAAAAGATAAAAAGAA
AAAAATGTCTAAACCAATGGATGTAGAACAAGAACCAAAAATTGATGATGCTTTATATAG
CAGACAACTTTATGCATTATCTCATGAAACAATGAAAAAAATTACATCAACTTCAGTTTT
AGTTGTAGGTTTACAAGGTCTTGGTATTGAAATTGTTAAAGATTTAAGTTTAGCAGGTGT
TAAATCTGTTACATTGTATGATAAAGAATTAGTTGAAATTAAAGATTTGTCTTCTCAATT
TTATTTTTCACCAGAACAAGTTGGTAAAGTTGGTAGAGCAGATGCATGTTTCCAAAAAGT
TGTAGATTTAAATAATTATGTTAGAATTGATGTACATAATGGAGAATTAAGTGATGAATT
CTTAAAGAAATTCAATGTTGTAGTATTAGCCAATCAACCATTAGCATTACAACTCAAAGT
GAATGAATTTTGTCATGCAAAT----------ACACTCAACAAGCTGGTGGTGATGCTGA
AGATGATCAATGTGATACCATTTTATCACAACTTCCNCCAACCAAGTGAAATGGCTGGTT
ACAAAATTAACTCTATTCAATTTGAAAAGGATGATGATACCAATCATCATATCGATTTCA
TTACCGCTACCTCAAATTTAAGAGCTACCAACTATGCAATCAGTCCAGCTGATAAACATA
AGACCAAAGGTATCGCCGGTAAAATCATTCCAGCTTTAGTTACCACTACTGCCGTAGTTG
CTGGTTTCGTTTGTATCGAATTGATTAAAGTTATTCAAAATAAAGCTTTGGAGAAATATA
AGAGCACCTTTATGAATTTAGGTATCCCATTCTTTGGTTTCGTTGAACCAATTGCCGCCC
CAAAGAACAAAATTCGTGAAGGCTGGACTTGGACACTTTGGGATCGTTTCGATGTTGATG
GTGATATCACTCTCAAGGAGTTTTTAGATCTCTTTGAAAAGAAACATGGTTTGGATATTT
CAATGCTCTCTTGTAAAGTTACACTTTTATATGCTCTCTTCACTGATAAGAAAACTAAAG
AGGAAAGATTAAAGATGAAAATCTCACAACTCTATGAAACCCTCTCAAAGAAACCATTAC
CAAAAGATAAGAAATATTTATTACTNAAATTTGTTGCAA