>SHF172 (SHF172Q) /CSM/SH/SHF1-C/SHF172Q.Seq.d/
XXXXXXXXXXTGTAAGAGAGTCAGAGAAACTGGTTTACAAATTTTAAATGTTTTCACTCG
TTTATCAAGTCAAAAATTGGATAAAATCTTAAAGAAAGGTTTAGAATCAATTCCAAATAA
TAATTGGTTAGTATTGAAAGAACCAAGAGATGTTAGATCAGTAAATGATATCTATTTAGA
AGAGATACTAAAATTCCAAAATGAAACTTCAAAACTTTTACCAGTTATAAATCATCATCA
TCATTCTTTAAAATCTCATTCAAGAACTGGTAGTGGTGGTAATAGTGTTTCTTCAAATAA
CTCTTCCTCGAATACACCAGATGGTCATAGAAGAAATCCTTCAGCATCATCATCATCATC
ATCAAATAATACAAATACAACTTTATTCGAAAAGAAAGTTGACCATGTCGATTTTAATAC
TCTTAGTGTTTTAATTGCTATCATTAAACTTTCACTTAAATCTTTTAATGAATCTTTAAG
ATTAAAAACTTTTGGTACAAATGGTTGTCATCAAATTCAAATTGATTTACATTATTTAAA
ATTATCATTATATGATTTATTTGGAAGTAATTCGTTTGATAATTTATTACAAGAATGTGA
AAATACTGTAACTGAAAGATGCGTTGACCCATTACCATTGGCAAAATCTATTGTATCAAA
AATTTGGAAACAAAAANAATAAAAAGAAAAATA

---KRVRETGLQILNVFTRLSSQKLDKILKKGLESIPNNNWLVLKEPRDVRSVNDIYLEE
ILKFQNETSKLLPVINHHHHSLKSHSRTGSGGNSVSSNNSSSNTPDGHRRNPSASSSSSS
NNTNTTLFEKKVDHVDFNTLSVLIAIIKLSLKSFNESLRLKTFGTNGCHQIQIDLHYLKL
SLYDLFGSNSFDNLLQECENTVTERCVDPLPLAKSIVSKIWKQKX*kek

Frame A:
---vreseklvykf*mfslvyqvknwiks*rkv*nqfqiiig*y*knqemldq*misi*k
ry*nskmklqnfyql*iiiiil*nliqelvvvvivflqitlprihqmvieeilqhhhhhh
qiiqiqlyskrkltmsilillvf*llslnfhlnllmnl*d*kllvqmvvikfkliyii*n
yhymiylevirliiyyknvkil*lkdalthyhwqnllyqkfgnkxnkkkn


Frame B:
---*esqrnwftnfkcfhsfikskig*nlkerfrinsk**lvsiertkrc*isk*ylfrr
dtkipk*nfktftsykssssffkisfknw*ww**cffk*lfleytrws*kksfsiiiiii
k*ykynfirkes*pcrf*ys*cfncyh*tft*if**ifkiknfwykwlssnsn*ftlfki
iii*fiwk*fv**fitrm*kycn*kmr*pitigkiyciknletkxikrki


Frame C:
---KRVRETGLQILNVFTRLSSQKLDKILKKGLESIPNNNWLVLKEPRDVRSVNDIYLEE
ILKFQNETSKLLPVINHHHHSLKSHSRTGSGGNSVSSNNSSSNTPDGHRRNPSASSSSSS
NNTNTTLFEKKVDHVDFNTLSVLIAIIKLSLKSFNESLRLKTFGTNGCHQIQIDLHYLKL
SLYDLFGSNSFDNLLQECENTVTERCVDPLPLAKSIVSKIWKQKX*kek

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHD589 (VHD589Q) /CSM/VH/VHD5-D/VHD589Q.Seq.d/                        662   0.0
VHD323 (VHD323Q) /CSM/VH/VHD3-A/VHD323Q.Seq.d/                        662   0.0
VHC475 (VHC475Q) /CSM/VH/VHC4-D/VHC475Q.Seq.d/                        662   0.0
VHB196 (VHB196Q) /CSM/VH/VHB1-D/VHB196Q.Seq.d/                        662   0.0
SHF172 (SHF172Q) /CSM/SH/SHF1-C/SHF172Q.Seq.d/                        662   0.0
CHG258 (CHG258Q) /CSM/CH/CHG2-C/CHG258Q.Seq.d/                        662   0.0
AHM617 (AHM617Q) /CSM/AH/AHM6-A/AHM617Q.Seq.d/                        662   0.0
AHK804 (AHK804Q) /CSM/AH/AHK8-A/AHK804Q.Seq.d/                        662   0.0
AHI481 (AHI481Q) /CSM/AH/AHI4-D/AHI481Q.Seq.d/                        662   0.0
AHB814 (AHB814Q) /CSM/AH/AHB8-A/AHB814Q.Seq.d/                        662   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC115598|AC115598.2 Dictyostelium discoideum chromosome 2 map 581427-735498 strain AX4, complete sequence.    40   0.030  5
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    34   0.035  9
AC116960|AC116960.2 Dictyostelium discoideum chromosome 2 map complement(1004496-821614) strain AX4, complete sequence.    34   0.048  8
CZ535855|CZ535855.1 SRAA-aac99h10.g1 Strongyloides ratti whole genome shotgun library (SRAAGSS 004) Strongyloides ratti genomic, genomic survey sequence.    40   0.17   2
AJ787693|AJ787693.1 Antirrhinum majus EST, clone 018_1_03_b07.     34   0.20   2
AJ792236|AJ792236.1 Antirrhinum majus EST, clone 018_2_10_p05.     34   0.20   2
BZ315065|BZ315065.1 ia53f11.b1 WGS-ZmaysF (JM107 adapted methyl filtered) Zea mays genomic clone ia53f11 5', DNA sequence.    48   0.26   1
BH659669|BH659669.1 BOMED45TR BO_2_3_KB Brassica oleracea genomic clone BOMED45, DNA sequence.    48   0.26   1
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.    36   0.35   8
AL049185|AL049185.4 Plasmodium falciparum DNA *** SEQUENCING IN PROGRESS *** from  MAL13P2.    36   0.36   9

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54KG3) RecName: Full=Protein fat-free homolog;                      370   e-101
AF124740_1(AF124740|pid:none) Zea mays clone CCMCB76 unknown mRNA.     98   2e-19
(Q155U0) RecName: Full=Protein fat-free;  &BC162801_1(BC162801|p...    98   3e-19
BT072770_1(BT072770|pid:none) Salmo salar clone ssal-rgf-539-352...    97   4e-19
BT058824_1(BT058824|pid:none) Salmo salar clone ssal-rgf-505-252...    97   4e-19
AK137155_1(AK137155|pid:none) Mus musculus adult male urinary bl...    90   7e-17
AK146211_1(AK146211|pid:none) Mus musculus CRL-1722 L5178Y-R cDN...    90   7e-17
BC010872_1(BC010872|pid:none) Homo sapiens chromosome 11 open re...    90   7e-17
(Q9UID3) RecName: Full=Protein fat-free homolog; AltName: Full=A...    90   7e-17
(A6QQ47) RecName: Full=Protein fat-free homolog;  &BC149640_1(BC...    90   7e-17

 psg: 0.83  gvh: 0.48  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.37  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.80  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: mitochondrial
	 40.0 %: nuclear
	 12.0 %: cytoplasmic
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for SHF172 is mit

>SHF172Z.Seq
NNNNNNNNNNTGTAAGAGAGTCAGAGAAACTGGTTTACAAATTTTAAATGTTTTCACTCG
TTTATCAAGTCAAAAATTGGATAAAATCTTAAAGAAAGGTTTAGAATCAATTCCAAATAA
TAATTGGTTAGTATTGAAAGAACCAAGAGATGTTAGATCAGTAAATGATATCTATTTAGA
AGAGATACTAAAATTCCAAAATGAAACTTCAAAACTTTTACCAGTTATAAATCATCATCA
TCATTCTTTAAAATCTCATTCAAGAACTGGTAGTGGTGGTAATAGTGTTTCTTCAAATAA
CTCTTCCTCGAATACACCAGATGGTCATAGAAGAAATCCTTCAGCATCATCATCATCATC
ATCAAATAATACAAATACAACTTTATTCGAAAAGAAAGTTGACCATGTCGATTTTAATAC
TCTTAGTGTTTTAATTGCTATCATTAAACTTTCACTTAAATCTTTTAATGAATCTTTAAG
ATTAAAAACTTTTGGTACAAATGGTTGTCATCAAATTCAAATTGATTTACATTATTTAAA
ATTATCATTATATGATTTATTTGGAAGTAATTCGTTTGATAATTTATTACAAGAATGTGA
AAATACTGTAACTGAAAGATGCGTTGACCCATTACCATTGGCAAAATCTATTGTATCAAA
AATTTGGAAACAAAAANAATAAAAAGAAAAATA