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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHF144
Library SH
(Link to library)
Clone ID SHF144
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16333-1
Original site URL
Representative seq. ID SHF144P
(Link to Original site)
Representative DNA sequence 
>SHF144 (SHF144Q) /CSM/SH/SHF1-B/SHF144Q.Seq.d/
AATAATTTAATTTTTTTCAAAAAAAATAATAATAATAATAATAAAAATAATAAAAAAAAT
AAATATTAATAAATATTATTTAAAAATTTTATTTATAGCAAAATAACTAATATATTGTAA
AATAATTTTAGAATATTTAAAAAATATTCATATAATAATAAATAAAAATGAAAGAATATA
AAAAAATTATATTATATAGTTTTTTAGTTTTTTTAATTTTATGTTTTGCAAAACCATCTC
TTTCAGATGACCCTTTAACAACTTCAACAACAACAACAACAGCAACTGCACTACCAACGA
CATCGGCAGCATCAACAGCAAAGGTAACAACAACAACCACAACTACAGGATCACCAGCAT
CAACCACAACTACAGGAGCACCAACATCAACTACAGGAGCATCAACATCAACTACAGGAG
CACCATCATCATCATCTTCATCGAATGAAACAACTCCAACTGCAACACCAACTAAAAAAC
CTGTAACACCAACACCATCATCAACATCATCATCATCATCATCATCTGAAGTAACATCAT
CCTCATCCGAATCATCAGCTGGTGAACATCCATGTGCAACTGATGTTTTTGATAGTATGA
GTAATTCTGGTAATTTCXXXXXXXXXXCATCGGAATATCCATACTATCAACTTGGTCAAT
TATTAAATGGTGCTTCCGATCAGTATAACNCTGGTAATGTAAATTTCAAATCCGTTAGTA
AACTTTTTAGAGGTTTTCCAACTATCGAGTATCNATTATACAATAATTACAAAGCNATCA
TTTCAATGATGAATGGTACNATCGATACATNTCAATATGATNTTTTGAGATTGAANTTNT
TNTTCCTTCATGATCAATNTTATAATAATCCAAATCCAAGTATTGANTTTAGTGAAGGTT
GCAAANTNTTTNTTTTTAGATNTTCAAATTTGTATTACCGTTCAAGTGGTTGGTAATTTA
CCTTGTGTTGGTTTATCNCCANACTTGGGTGGTCAAGTTCCAAATGCAGTATGGGTTGAA
CTTTTAANTCCTTTNTNTGATNTTCAAGTTTATCCAAATGNTTTTATCAGTAGTATCNCT
TTCCAAANTCAAGTACAAGATCNCCAAGGTGTTTTNTTTANTGGTGATANTTNTGCTGGT
AATTTTGTACCNCCATATAAAGTA
sequence update 2002.10.25
Translated Amino Acid sequence 
*fnffqkk******k**kk*ilinii*kfyl*qnn*yivk*f*ni*kifi***IKMKEYK
KIILYSFLVFLILCFAKPSLSDDPLTTSTTTTTATALPTTSAASTAKVTTTTTTTGSPAS
TTTTGAPTSTTGASTSTTGAPSSSSSSNETTPTATPTKKPVTPTPSSTSSSSSSSEVTSS
SSESSAGEHPCATDVFDSMSNSGNF---

---SEYPYYQLGQLLNGASDQYNXGNVNFKSVSKLFRGFPTIEYXLYNNYKAIISMMNGT
IDTXQYDXLRLXXXFLHDQXYNNPNPSIXFSEGCKXFXFRXSNLYYRSSGW*ftlcwfix
xlgwssskcsmg*tfxsfx*xsslskxfyq*yxfpxsstrsprcfxxw*xxcw*fctxi*
s
Translated Amino Acid sequence (All Frames) 
Frame A:
nnliffkknnnnnnknnkknky**ilfknfiyskitnil*nnfrifkkysynnk*k*kni
kklyyivf*ff*fyvlqnhlfqmtl*qlqqqqqqqlhyqrhrqhqqqr*qqqpqlqdhqh
qpqlqehqhqlqehqhqlqehhhhhlhrmkqlqlqhqlknl*hqhhhqhhhhhhhlk*hh
phpnhqlvnihvqlmfliv*vilvi---

---hrnihtinlvny*mvlpisitlvm*isnplvnflevfqlssixytiitkxsfq**mv
xsihxnmxf*d*xxxsfminxiiiqiqvlxlvkvaxxxfldxqicitvqvvgnlpcvgls
pxlggqvpnavwvellxpxxdxqvypnxfissixfqxqvqdxqgvxfxgdxxagnfvppy
kv

Frame B:
ii*ffskkiiiiiikiikkininkyylkilfiak*liyckiileylknihiiinkneri*
knyii*ffsffnfmfcktisfr*pfnnfnnnnnsncttndigsinskgnnnnhnyritsi
nhnyrstninyrsininyrstiiiifie*nnsncntn*ktcntntiiniiiiiii*snii
liriisw*tsmcn*cf**ye*fw*f---

---igisilstwsiikwcfrsv*xw*ckfqir**tf*rfsnyrvsiiq*lqsxhfndewy
xryxsi*xfeiexxxps*sxl**sksky*x**rlqxxxf*xfkfvlpfkwlviylvlvyx
xtwvvkfqmqyglnf*xlxxxfkfiqmxlsvvslskxkykixkvfxlxvixxlvilyxhi
k

Frame C:
*fnffqkk******k**kk*ilinii*kfyl*qnn*yivk*f*ni*kifi***IKMKEYK
KIILYSFLVFLILCFAKPSLSDDPLTTSTTTTTATALPTTSAASTAKVTTTTTTTGSPAS
TTTTGAPTSTTGASTSTTGAPSSSSSSNETTPTATPTKKPVTPTPSSTSSSSSSSEVTSS
SSESSAGEHPCATDVFDSMSNSGNF---

---SEYPYYQLGQLLNGASDQYNXGNVNFKSVSKLFRGFPTIEYXLYNNYKAIISMMNGT
IDTXQYDXLRLXXXFLHDQXYNNPNPSIXFSEGCKXFXFRXSNLYYRSSGW*ftlcwfix
xlgwssskcsmg*tfxsfx*xsslskxfyq*yxfpxsstrsprcfxxw*xxcw*fctxi*
s
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHF144 (SHF144Q) /CSM/SH/SHF1-B/SHF144Q.Seq.d/                        918   0.0
CHJ125 (CHJ125Q) /CSM/CH/CHJ1-B/CHJ125Q.Seq.d/                        813   0.0
CHQ321 (CHQ321Q) /CSM/CH/CHQ3-A/CHQ321Q.Seq.d/                        811   0.0
CHJ322 (CHJ322Q) /CSM/CH/CHJ3-A/CHJ322Q.Seq.d/                        811   0.0
SHJ588 (SHJ588Q) /CSM/SH/SHJ5-D/SHJ588Q.Seq.d/                        805   0.0
CHR283 (CHR283Q) /CSM/CH/CHR2-D/CHR283Q.Seq.d/                        799   0.0
CHI503 (CHI503Q) /CSM/CH/CHI5-A/CHI503Q.Seq.d/                        799   0.0
AHM661 (AHM661Q) /CSM/AH/AHM6-C/AHM661Q.Seq.d/                        799   0.0
CHR237 (CHR237Q) /CSM/CH/CHR2-B/CHR237Q.Seq.d/                        797   0.0
AHI234 (AHI234Q) /CSM/AH/AHI2-B/AHI234Q.Seq.d/                        791   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC114263|AC114263.2 Dictyostelium discoideum chromosome 2 map 215673-367476 strain AX4, complete sequence.    40   0.005  2
AC126867|AC126867.4 Rattus norvegicus clone CH230-49B18, WORKING DRAFT SEQUENCE, 1 ordered piece.    54   0.006  3
AC095285|AC095285.5 Rattus norvegicus clone CH230-11A8, WORKING DRAFT SEQUENCE, 1 ordered piece.    54   0.006  3
BP663205|BP663205.1 Arabidopsis thaliana cDNA clone:RAFL21-47-C10, 3' end.    38   0.018  2
DT505060|DT505060.1 WS01315.B21_O02 PTxD-IL-FL-A-4 Populus trichocarpa x Populus deltoides cDNA clone WS01315_O02 3', mRNA sequence.    38   0.086  2
BJ008043|BJ008043.1 Oryzias latipes cDNA clone:MF01SSA114H01 : 5' end, single read.    34   0.087  2
CN518273|CN518273.1 GQ0093.B3_H17 GQ009 Populus balsamifera subsp. trichocarpa x Populus deltoides cDNA clone GQ0093_H17 5', mRNA sequence.    38   0.089  2
AF310888|AF310888.1 Dictyostelium discoideum RacC (racC) gene, complete cds, alternatively spliced; and unknown gene.    34   0.11   4
DW660731|DW660731.1 CNB304-H09.y1d-s SHGC-CNB2 Gasterosteus aculeatus cDNA clone CNB304-H09 5', mRNA sequence.    50   0.11   1
AC123513|AC123513.1 Dictyostelium discoideum chromosome 2 map 2779865-2840915 strain AX4, *** SEQUENCING IN PROGRESS ***, in ordered pieces.    36   0.15   5
dna update 2006. 3.11
Homology vs Protein 

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 837,716,363
protein update 2009. 4.18
PSORT 

 psg: 1.00  gvh: 0.82  alm: 0.55  top: 0.20  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	 12.0 %: endoplasmic reticulum
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: nuclear

>> prediction for SHF144 is exc
5' end seq. ID SHF144F
5' end seq. 
>SHF144F.Seq
AATAATTTAATTTTTTTCAAAAAAAATAATAATAATAATAATAAAAATAATAAAAAAAAT
AAATATTAATAAATATTATTTAAAAATTTTATTTATAGCAAAATAACTAATATATTGTAA
AATAATTTTAGAATATTTAAAAAATATTCATATAATAATAAATAAAAATGAAAGAATATA
AAAAAATTATATTATATAGTTTTTTAGTTTTTTTAATTTTATGTTTTGCAAAACCATCTC
TTTCAGATGACCCTTTAACAACTTCAACAACAACAACAACAGCAACTGCACTACCAACGA
CATCGGCAGCATCAACAGCAAAGGTAACAACAACAACCACAACTACAGGATCACCAGCAT
CAACCACAACTACAGGAGCACCAACATCAACTACAGGAGCATCAACATCAACTACAGGAG
CACCATCATCATCATCTTCATCGAATGAAACAACTCCAACTGCAACACCAACTAAAAAAC
CTGTAACACCAACACCATCATCAACATCATCATCATCATCATCATCTGAAGTAACATCAT
CCTCATCCGAATCATCAGCTGGTGAACATCCATGTGCAACTGATGTTTTTGATAGTATGA
GTAATTCTGGTAATTTCNNNNNNNNNN
Length of 5' end seq. 627
3' end seq. ID SHF144Z
3' end seq. 
>SHF144Z.Seq
NNNNNNNNNNCATCGGAATATCCATACTATCAACTTGGTCAATTATTAAATGGTGCTTCC
GATCAGTATAACNCTGGTAATGTAAATTTCAAATCCGTTAGTAAACTTTTTAGAGGTTTT
CCAACTATCGAGTATCNATTATACAATAATTACAAAGCNATCATTTCAATGATGAATGGT
ACNATCGATACATNTCAATATGATNTTTTGAGATTGAANTTNTTNTTCCTTCATGATCAA
TNTTATAATAATCCAAATCCAAGTATTGANTTTAGTGAAGGTTGCAAANTNTTTNTTTTT
AGATNTTCAAATTTGTATTACCGTTCAAGTGGTTGGTAATTTACCTTGTGTTGGTTTATC
NCCANACTTGGGTGGTCAAGTTCCAAATGCAGTATGGGTTGAACTTTTAANTCCTTTNTN
TGATNTTCAAGTTTATCCAAATGNTTTTATCAGTAGTATCNCTTTCCAAANTCAAGTACA
AGATCNCCAAGGTGTTTTNTTTANTGGTGATANTTNTGCTGGTAATTTTGTACCNCCATA
TAAAGTA
Length of 3' end seq. 547
Connected seq. ID SHF144P
Connected seq. 
>SHF144P.Seq
AATAATTTAATTTTTTTCAAAAAAAATAATAATAATAATAATAAAAATAATAAAAAAAAT
AAATATTAATAAATATTATTTAAAAATTTTATTTATAGCAAAATAACTAATATATTGTAA
AATAATTTTAGAATATTTAAAAAATATTCATATAATAATAAATAAAAATGAAAGAATATA
AAAAAATTATATTATATAGTTTTTTAGTTTTTTTAATTTTATGTTTTGCAAAACCATCTC
TTTCAGATGACCCTTTAACAACTTCAACAACAACAACAACAGCAACTGCACTACCAACGA
CATCGGCAGCATCAACAGCAAAGGTAACAACAACAACCACAACTACAGGATCACCAGCAT
CAACCACAACTACAGGAGCACCAACATCAACTACAGGAGCATCAACATCAACTACAGGAG
CACCATCATCATCATCTTCATCGAATGAAACAACTCCAACTGCAACACCAACTAAAAAAC
CTGTAACACCAACACCATCATCAACATCATCATCATCATCATCATCTGAAGTAACATCAT
CCTCATCCGAATCATCAGCTGGTGAACATCCATGTGCAACTGATGTTTTTGATAGTATGA
GTAATTCTGGTAATTTC----------CATCGGAATATCCATACTATCAACTTGGTCAAT
TATTAAATGGTGCTTCCGATCAGTATAACNCTGGTAATGTAAATTTCAAATCCGTTAGTA
AACTTTTTAGAGGTTTTCCAACTATCGAGTATCNATTATACAATAATTACAAAGCNATCA
TTTCAATGATGAATGGTACNATCGATACATNTCAATATGATNTTTTGAGATTGAANTTNT
TNTTCCTTCATGATCAATNTTATAATAATCCAAATCCAAGTATTGANTTTAGTGAAGGTT
GCAAANTNTTTNTTTTTAGATNTTCAAATTTGTATTACCGTTCAAGTGGTTGGTAATTTA
CCTTGTGTTGGTTTATCNCCANACTTGGGTGGTCAAGTTCCAAATGCAGTATGGGTTGAA
CTTTTAANTCCTTTNTNTGATNTTCAAGTTTATCCAAATGNTTTTATCAGTAGTATCNCT
TTCCAAANTCAAGTACAAGATCNCCAAGGTGTTTTNTTTANTGGTGATANTTNTGCTGGT
AATTTTGTACCNCCATATAAAGTA
Length of connected seq. 1154
Full length Seq ID -
Full length Seq. -
Length of full length seq. -