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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHF132
Library SH
(Link to library)
Clone ID SHF132
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U11380-1
Original site URL
Representative seq. ID SHF132P
(Link to Original site)
Representative DNA sequence 
>SHF132 (SHF132Q) /CSM/SH/SHF1-B/SHF132Q.Seq.d/
TTCTTCAATTACTTCATCATTTAATAAACTTAATATTATACCAACACCAGTAGCTGCAGT
AGAACCAAAAAAGAGAACAAAAGAAGCAGTAGCTTCAGTTCCAATAGCATCAATATCTAC
ATTTGTTCCTTTACAAGTACCAACACCACCAACATCAGTATTCTTCCCAACAACAACAAC
AACAACACCGGTTGCCACAACAAATTTAGAATCATCAATGAAATTTAATGAATTTGTGAC
ATCATTTAATAATATTTCAATTGGTGATAAATCAATTTATCAAGAAGATTCAACTGAAAA
TGAATATAGAAAATTATTTGTAAATAATCGTAATGATTCAAAATTGAATAATTTATATTT
ACATTGTGAAAAGGTATTTAAAAGTAAACCAACCACCTCTGAATTACCAAATGTGAATGG
TGTCGATTGTATTATGGGATTCAATGCAAAACAACATTCATTTTCAAATGATATCAAGTT
TAAACAAGAATGGAATAGATTTAGTAATAATATGTTTGATGGTTTCAATTGGTCCAATGT
ATTTATTXXXXXXXXXXGTGGTGGTTTTGGTAGTACTGNTCAATCATTGGGAGGACCAGG
TCAATCATTTGGAGGTCCAGGTCAATCATTCGGTCCAAAACCAATTGCAAGTGGTGGTTT
TGGTAGTACTGCTATACCATTTGGAGGACCAGGTCAATCATTTGGAGGATCGGCTACAAG
TGGTGGCTTTGGTAGCACTGCTCAATCATTTGGAGGTCCAAAACCAACCACAATTGGAAG
CAGTGGTTTTGGTAGTACTGCTGTATCATTTGGAGGACCAGGTCAACCATTTGGAGGATC
GGCCACAAGTGGTGGTTTTGGATCATCAACACCACAATCAACTCCAGCTTTTCCAAGTTC
ATTTGGAATTGGAGGTAAATTTGCTGCTAAAATTCCACCATCAGCCTCTATCCCTGCTAG
TGGTTTTGTCCCTCTTGCCTCAAACATATCTGCAACTCCAACTGGTTTATTCAGCAATAC
CCAACATTCTCTAAAAAATATTCCACAAAATATGGGTGGTAGTAGTGTATCAAACGTTTN
TCTTTCTATTGCTAAGCCTGCCACACTACTCAATTCTACACAATTCTTGGAATCCTCAGT
TATGGCTCAATGTTTATACTTGGTAGATGATTTATCAACAACAAAGAAATTACCAGATCA
AACCGTAAAAAGAATTAAAGAATTACTTTTCTCTCTTTATTTACCAATCTTTAATTGTTT
TGAAGCTTTCCTTTTAAATACAAATGAAAAAGCTTTAATTGAGAATCTTTTATGGCTTTA
AAAAATTANATCAAAAACAATA
sequence update 2002.10.25
Translated Amino Acid sequence 
SSITSSFNKLNIIPTPVAAVEPKKRTKEAVASVPIASISTFVPLQVPTPPTSVFFPTTTT
TTPVATTNLESSMKFNEFVTSFNNISIGDKSIYQEDSTENEYRKLFVNNRNDSKLNNLYL
HCEKVFKSKPTTSELPNVNGVDCIMGFNAKQHSFSNDIKFKQEWNRFSNNMFDGFNWSNV
FI---

---GGFGSTXQSLGGPGQSFGGPGQSFGPKPIASGGFGSTAIPFGGPGQSFGGSATSGGF
GSTAQSFGGPKPTTIGSSGFGSTAVSFGGPGQPFGGSATSGGFGSSTPQSTPAFPSSFGI
GGKFAAKIPPSASIPASGFVPLASNISATPTGLFSNTQHSLKNIPQNMGGSSVSNVXLSI
AKPATLLNSTQFLESSVMAQCLYLVDDLSTTKKLPDQTVKRIKELLFSLYLPIFNCFEAF
LLNTNEKALIENLLWL*kixskti
Translated Amino Acid sequence (All Frames) 
Frame A:
ffnyfii**t*yytntsscsrtkkenkrsssfssnsiniyicsftstnttnisilpnnnn
nntgchnkfriinei**icdii**yfnw**inlsrrfn*k*i*kiick*s**fkie*fif
tl*kgi*k*tnhl*itkcewcrlyygiqckttfifk*yqv*trme*i***yv*wfqlvqc
iy---

---vvvlvvlxnhwedqvnhlevqvnhsvqnqlqvvvlvvllyhledqvnhledrlqvva
lvallnhlevqnqpqleavvlvvllyhledqvnhledrpqvvvldhqhhnqlqlfqvhle
levnlllkfhhqplsllvvlsllpqtylqlqlvysaipnil*kifhkiwvvvvyqtfxfl
llslphysilhnswnpqlwlnvytw*miyqqqrnyqikp*kelknyfslfiyqslivlkl
sf*iqmkkl*lrifygfkklxqkq

Frame B:
SSITSSFNKLNIIPTPVAAVEPKKRTKEAVASVPIASISTFVPLQVPTPPTSVFFPTTTT
TTPVATTNLESSMKFNEFVTSFNNISIGDKSIYQEDSTENEYRKLFVNNRNDSKLNNLYL
HCEKVFKSKPTTSELPNVNGVDCIMGFNAKQHSFSNDIKFKQEWNRFSNNMFDGFNWSNV
FI---

---wwfw*yxsiigrtrsiiwrsrsiirsktnckwwfw*ycytiwrtrsiiwrigykwwl
w*hcsiiwrsktnhnwkqwfw*ycciiwrtrstiwrighkwwfwiinttinssfskfiwn
wr*icc*nstislypc*wfcpsclkhicnsnwfiqqyptfskkystkygw**cikrxsfy
c*achttqfytilgilsygsmfilgr*finnkeitrsnrkkn*ritflslftnl*lf*sf
pfkyk*ksfn*esfmalknxiknn

Frame C:
lqllhhlinlilyqhq*lq*nqkreqkkq*lqfq*hqylhlflykyqhhqhqyssqqqqq
qhrlpqqi*nhq*nlmnl*hhliifqlvinqfikkiqlkmnienyl*iivmiqn*iiyiy
ivkrylkvnqpplnyqm*mvsivlwdsmqnnihfqmisslnkngidlviiclmvsigpmy
l---

---GGFGSTXQSLGGPGQSFGGPGQSFGPKPIASGGFGSTAIPFGGPGQSFGGSATSGGF
GSTAQSFGGPKPTTIGSSGFGSTAVSFGGPGQPFGGSATSGGFGSSTPQSTPAFPSSFGI
GGKFAAKIPPSASIPASGFVPLASNISATPTGLFSNTQHSLKNIPQNMGGSSVSNVXLSI
AKPATLLNSTQFLESSVMAQCLYLVDDLSTTKKLPDQTVKRIKELLFSLYLPIFNCFEAF
LLNTNEKALIENLLWL*kixskti
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHF132 (SHF132Q) /CSM/SH/SHF1-B/SHF132Q.Seq.d/                       2212   0.0
SHF635 (SHF635Q) /CSM/SH/SHF6-B/SHF635Q.Seq.d/                       1475   0.0
VHK513 (VHK513Q) /CSM/VH/VHK5-A/VHK513Q.Seq.d/                       1463   0.0
VHO252 (VHO252Q) /CSM/VH/VHO2-C/VHO252Q.Seq.d/                       1413   0.0
VHD643 (VHD643Q) /CSM/VH/VHD6-B/VHD643Q.Seq.d/                       1328   0.0
AHH474 (AHH474Q) /CSM/AH/AHH4-D/AHH474Q.Seq.d/                       1035   0.0
VHF420 (VHF420Q) /CSM/VH/VHF4-A/VHF420Q.Seq.d/                        963   0.0
VHI446 (VHI446Q) /CSM/VH/VHI4-B/VHI446Q.Seq.d/                        757   0.0
VHD142 (VHD142Q) /CSM/VH/VHD1-B/VHD142Q.Seq.d/                        583   e-165
VHK243 (VHK243Q) /CSM/VH/VHK2-B/VHK243Q.Seq.d/                        537   e-151
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AM043557|AM043557.1 Schistosoma mansoni lung schistosomulum EST, clone SmlC42h02.q1k.    54   0.008  1
CD256609|CD256609.1 AGENCOURT_14165334 NICHD_XGC_Brn1 Xenopus laevis cDNA clone IMAGE:6951750 5', mRNA sequence.    38   0.034  3
CP000084|CP000084.1 Candidatus Pelagibacter ubique HTCC1062, complete genome.    36   0.041  21
AC109060|AC109060.4 Rattus norvegicus clone CH230-95O6, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    50   0.11   3
AC141784|AC141784.1 Apis mellifera clone CH224-59E8, WORKING DRAFT SEQUENCE, 31 unordered pieces.    44   0.11   2
AC176693|AC176693.1 Strongylocentrotus purpuratus clone R3-3088G04, WORKING DRAFT SEQUENCE, 17 unordered pieces.    46   0.12   6
CD154636|CD154636.1 ML1-0036T-L175-B10-U.B ML1-0036 Schistosoma mansoni cDNA clone ML1-0036T-L175-B10.B, mRNA sequence.    50   0.13   1
AF547983|AF547983.1 Schizosaccharomyces japonicus mitochondrial DNA, complete genome.    38   0.34   6
BM278816|BM278816.1 As_tgz_67G12_SKPL Ascaris suum adult male testis germinal zone from Alan Scott Ascaris suum cDNA clone As_tgz_67G12 5', mRNA sequence.    36   0.42   2
CT188711|CT188711.1 Sus scrofa genomic clone PigE-74G17, genomic survey sequence.    48   0.52   1
dna update 2006. 3.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AL844502_79(AL844502|pid:none) Plasmodium falciparum chromosome ...    36   3.4
AL844508_65(AL844508|pid:none) Plasmodium falciparum strain 3D7,...    35   4.5
BC047016_1(BC047016|pid:none) Homo sapiens FCH and double SH3 do...    35   5.8
CP000481_1655(CP000481|pid:none) Acidothermus cellulolyticus 11B...    35   7.6
(Q4QM23) RecName: Full=Thiol:disulfide interchange protein dsbD;...    34   9.9
AL844508_170(AL844508|pid:none) Plasmodium falciparum strain 3D7...    34   10.0
protein update 2009. 4.18
PSORT 

 psg: 0.89  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.32  mip: 0.00
 nuc: 0.09  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 28.0 %: nuclear
	 16.0 %: mitochondrial
	  4.0 %: plasma membrane

>> prediction for SHF132 is cyt
5' end seq. ID SHF132F
5' end seq. 
>SHF132F.Seq
TTCTTCAATTACTTCATCATTTAATAAACTTAATATTATACCAACACCAGTAGCTGCAGT
AGAACCAAAAAAGAGAACAAAAGAAGCAGTAGCTTCAGTTCCAATAGCATCAATATCTAC
ATTTGTTCCTTTACAAGTACCAACACCACCAACATCAGTATTCTTCCCAACAACAACAAC
AACAACACCGGTTGCCACAACAAATTTAGAATCATCAATGAAATTTAATGAATTTGTGAC
ATCATTTAATAATATTTCAATTGGTGATAAATCAATTTATCAAGAAGATTCAACTGAAAA
TGAATATAGAAAATTATTTGTAAATAATCGTAATGATTCAAAATTGAATAATTTATATTT
ACATTGTGAAAAGGTATTTAAAAGTAAACCAACCACCTCTGAATTACCAAATGTGAATGG
TGTCGATTGTATTATGGGATTCAATGCAAAACAACATTCATTTTCAAATGATATCAAGTT
TAAACAAGAATGGAATAGATTTAGTAATAATATGTTTGATGGTTTCAATTGGTCCAATGT
ATTTATTNNNNNNNNNN
Length of 5' end seq. 557
3' end seq. ID SHF132Z
3' end seq. 
>SHF132Z.Seq
NNNNNNNNNNGTGGTGGTTTTGGTAGTACTGNTCAATCATTGGGAGGACCAGGTCAATCA
TTTGGAGGTCCAGGTCAATCATTCGGTCCAAAACCAATTGCAAGTGGTGGTTTTGGTAGT
ACTGCTATACCATTTGGAGGACCAGGTCAATCATTTGGAGGATCGGCTACAAGTGGTGGC
TTTGGTAGCACTGCTCAATCATTTGGAGGTCCAAAACCAACCACAATTGGAAGCAGTGGT
TTTGGTAGTACTGCTGTATCATTTGGAGGACCAGGTCAACCATTTGGAGGATCGGCCACA
AGTGGTGGTTTTGGATCATCAACACCACAATCAACTCCAGCTTTTCCAAGTTCATTTGGA
ATTGGAGGTAAATTTGCTGCTAAAATTCCACCATCAGCCTCTATCCCTGCTAGTGGTTTT
GTCCCTCTTGCCTCAAACATATCTGCAACTCCAACTGGTTTATTCAGCAATACCCAACAT
TCTCTAAAAAATATTCCACAAAATATGGGTGGTAGTAGTGTATCAAACGTTTNTCTTTCT
ATTGCTAAGCCTGCCACACTACTCAATTCTACACAATTCTTGGAATCCTCAGTTATGGCT
CAATGTTTATACTTGGTAGATGATTTATCAACAACAAAGAAATTACCAGATCAAACCGTA
AAAAGAATTAAAGAATTACTTTTCTCTCTTTATTTACCAATCTTTAATTGTTTTGAAGCT
TTCCTTTTAAATACAAATGAAAAAGCTTTAATTGAGAATCTTTTATGGCTTTAAAAAATT
ANATCAAAAACAATA
Length of 3' end seq. 795
Connected seq. ID SHF132P
Connected seq. 
>SHF132P.Seq
TTCTTCAATTACTTCATCATTTAATAAACTTAATATTATACCAACACCAGTAGCTGCAGT
AGAACCAAAAAAGAGAACAAAAGAAGCAGTAGCTTCAGTTCCAATAGCATCAATATCTAC
ATTTGTTCCTTTACAAGTACCAACACCACCAACATCAGTATTCTTCCCAACAACAACAAC
AACAACACCGGTTGCCACAACAAATTTAGAATCATCAATGAAATTTAATGAATTTGTGAC
ATCATTTAATAATATTTCAATTGGTGATAAATCAATTTATCAAGAAGATTCAACTGAAAA
TGAATATAGAAAATTATTTGTAAATAATCGTAATGATTCAAAATTGAATAATTTATATTT
ACATTGTGAAAAGGTATTTAAAAGTAAACCAACCACCTCTGAATTACCAAATGTGAATGG
TGTCGATTGTATTATGGGATTCAATGCAAAACAACATTCATTTTCAAATGATATCAAGTT
TAAACAAGAATGGAATAGATTTAGTAATAATATGTTTGATGGTTTCAATTGGTCCAATGT
ATTTATT----------GTGGTGGTTTTGGTAGTACTGNTCAATCATTGGGAGGACCAGG
TCAATCATTTGGAGGTCCAGGTCAATCATTCGGTCCAAAACCAATTGCAAGTGGTGGTTT
TGGTAGTACTGCTATACCATTTGGAGGACCAGGTCAATCATTTGGAGGATCGGCTACAAG
TGGTGGCTTTGGTAGCACTGCTCAATCATTTGGAGGTCCAAAACCAACCACAATTGGAAG
CAGTGGTTTTGGTAGTACTGCTGTATCATTTGGAGGACCAGGTCAACCATTTGGAGGATC
GGCCACAAGTGGTGGTTTTGGATCATCAACACCACAATCAACTCCAGCTTTTCCAAGTTC
ATTTGGAATTGGAGGTAAATTTGCTGCTAAAATTCCACCATCAGCCTCTATCCCTGCTAG
TGGTTTTGTCCCTCTTGCCTCAAACATATCTGCAACTCCAACTGGTTTATTCAGCAATAC
CCAACATTCTCTAAAAAATATTCCACAAAATATGGGTGGTAGTAGTGTATCAAACGTTTN
TCTTTCTATTGCTAAGCCTGCCACACTACTCAATTCTACACAATTCTTGGAATCCTCAGT
TATGGCTCAATGTTTATACTTGGTAGATGATTTATCAACAACAAAGAAATTACCAGATCA
AACCGTAAAAAGAATTAAAGAATTACTTTTCTCTCTTTATTTACCAATCTTTAATTGTTT
TGAAGCTTTCCTTTTAAATACAAATGAAAAAGCTTTAATTGAGAATCTTTTATGGCTTTA
AAAAATTANATCAAAAACAATA
Length of connected seq. 1332
Full length Seq ID -
Full length Seq. -
Length of full length seq. -