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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFJ307
Library AF
(Link to library)
Clone ID AFJ307
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U09887-1
Original site URL
Representative seq. ID AFJ307P
(Link to Original site)
Representative DNA sequence 
>AFJ307 (AFJ307Q) /CSM/AF/AFJ3-A/AFJ307Q.Seq.d/
CACTGTTGGCCTACTGGAACACTGTATATTGGTATGGGTGTTTTCAGAAAACACAACGAA
TTTATATACACCAAATAATTACAATGATTTCTTTATCAAAGAATTTTTCGAAGAATGGTT
ATTCTATAATCAAAAAGTATAATAAAATATCATCACCAAAAGCAATTATTGGAAATCCAT
CAATTTCCATAAAATCATCGTCATCATTTATTTTTAATATTAAAAATAACAACAACAACA
ACATTGGTACATCAAATACACTTAATATTAAAAGATTTTATAGTGAGGCTAATAATAAAA
ATAATAATGTTAATCAAGATAAAAAACAACAACAACAAAATTTTGATAATGATGATACTT
ATCATAATAAAGAAGAATTTACAAAACCACCAAAATATTTTTATGCTGTTGCATTATTAG
CTATTGGATGTATTGGAACTTATTTAGTAGATAATTTTATAAGTTTTAGTAATGAACAAA
TTTTAAGAGATATTAAAACAATTTTAAAGAGTGAAGATGCATTAGAAAGAAATAATGCAT
TGAAATCAATATCAACTAGAGCAGGCCAAGCATTTTGTGTTGAAAAAGTATTTAAATTAG
GTGTTGXXXXXXXXXXGAAGCTGCTTCAAATTTATGGTTTCATTATTTTTTATCAAAAAC
TAATCAAATTCAATTATTAACATCCAAATGATTTATTATATTGTTTGCAAAATTTAGCAT
ATTCTGATAAATTTGAATTACAAAGAATATCAATTGCAACAGTAAAGATATTATTTGCAA
ATCGTGAACTTTATCCAATGTTAAATAAATTGAAAGGAATGATATCACATTTAAATAAGA
GTCAAGATGTTTTAATTTCAAATCATGCTGAATATTATTTAAATGTACTCAAAGGTGAAT
ATATTCCACCAGTTGGAAATATTTCTTTACCCGATGAAGAATTAGATCGTAAATTTAAAA
TTCATTCATGTTATAATTTATTATTGGCACCATTAACTTATTTATATTGTTGTTCAAGAT
GGAAAAAATATACAAGAGATCCAATTTATTTAAAAGCAAAGGGTATTTATGGTGGTACAA
TGATGTTATTAGCAGCAGTTATTTCATCATTGACCCTTTTTCCAGATTATCATCCATCAA
AGATTAATGCAAGATTAAAGAAAGAATTTTCATTAAATACAAAAACAATTGAAGAATTTG
AAAGATGTAAAGCAATTTCATTAGCTGAAGCTGTTACTTTAGTNATAAAAATAAAAATAA
TAATAATAATAATAATAATAATAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
LLAYWNTVYWYGCFQKTQRIYIHQIITMISLSKNFSKNGYSIIKKYNKISSPKAIIGNPS
ISIKSSSSFIFNIKNNNNNNIGTSNTLNIKRFYSEANNKNNNVNQDKKQQQQNFDNDDTY
HNKEEFTKPPKYFYAVALLAIGCIGTYLVDNFISFSNEQILRDIKTILKSEDALERNNAL
KSISTRAGQAFCVEKVFKLGV---

---EAASNLWFHYFLSKTNQIQLLTSK*fiilfakfsif**i*itknincnskdiicks*
tlsnvk*iernditfk*esrcfnfksc*ilfkctqr*iystswkyfftr*rirs*i*nsf
ml*fiigtinlfillfkmekiykrsnlfkskgylwwyndvisssyfiidpfsrlssikd*
ckikerifikyknn*ri*km*snfis*scyfsxknknnnnnnnnn
Translated Amino Acid sequence (All Frames) 
Frame A:
hcwptgtlyigmgvfrkhnefiytk*lq*flyqrifrrmvil*sksiikyhhqkqlleih
qfp*nhrhhlflilkitttttlvhqihlilkdfivrliikiimlikiknnnnkilimmil
iiikknlqnhqnifmllhy*lldvleli**iil*vlvmnkf*eilkqf*rvkmh*keimh
*nqyqleqakhfvlkkyln*vl---

---EAASNLWFHYFLSKTNQIQLLTSK*fiilfakfsif**i*itknincnskdiicks*
tlsnvk*iernditfk*esrcfnfksc*ilfkctqr*iystswkyfftr*rirs*i*nsf
ml*fiigtinlfillfkmekiykrsnlfkskgylwwyndvisssyfiidpfsrlssikd*
ckikerifikyknn*ri*km*snfis*scyfsxknknnnnnnnnn

Frame B:
tvgllehcilvwvfsenttnlytpnnyndffikeffeewlfynqkv**niitksnywksi
nfhkiiviiyf*y*k*qqqqhwyikyt*y*kil**g***k**c*sr*kttttkf****yl
s**rriykttkiflccciisywmywnlfsr*fykf***tnfkry*nnfke*rcirkk*ci
einin*srpsilc*ksi*irc---

---kllqiygfiifyqklikfny*hpndllyclqnlaysdkfelqrisiatvkilfanre
lypmlnklkgmishlnksqdvlisnhaeyylnvlkgeyippvgnislpdeeldrkfkihs
cynlllapltylyccsrwkkytrdpiylkakgiyggtmmllaavissltlfpdyhpskin
arlkkefslntktieeferckaislaeavtlvikikiiiiiiiii

Frame C:
LLAYWNTVYWYGCFQKTQRIYIHQIITMISLSKNFSKNGYSIIKKYNKISSPKAIIGNPS
ISIKSSSSFIFNIKNNNNNNIGTSNTLNIKRFYSEANNKNNNVNQDKKQQQQNFDNDDTY
HNKEEFTKPPKYFYAVALLAIGCIGTYLVDNFISFSNEQILRDIKTILKSEDALERNNAL
KSISTRAGQAFCVEKVFKLGV---

---scfkfmvslffikn*snsiiniqmiyyivcki*hilinlnykeyqlqq*ryylqivn
fiqc*in*ke*yhi*irvkmf*fqimlnii*myskvnifhqleiflypmkn*ivnlkfih
viiyywhh*liyivvqdgkniqeiqfi*kqrvfmvvq*cy*qqlfhh*pffqiiihqrlm
qd*rknfh*iqkqlknlkdvkqfh*lklll*x*k*k*********
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFJ307 (AFJ307Q) /CSM/AF/AFJ3-A/AFJ307Q.Seq.d/                       1729   0.0
VFJ834 (VFJ834Q) /CSM/VF/VFJ8-B/VFJ834Q.Seq.d/                       1227   0.0
AFB387 (AFB387Q) /CSM/AF/AFB3-D/AFB387Q.Seq.d/                       1213   0.0
AFJ537 (AFJ537Q) /CSM/AF/AFJ5-B/AFJ537Q.Seq.d/                       1197   0.0
SFC656 (SFC656Q) /CSM/SF/SFC6-C/SFC656Q.Seq.d/                       1138   0.0
AFL341 (AFL341Q) /CSM/AF/AFL3-B/AFL341Q.Seq.d/                        771   0.0
AFM123 (AFM123Q) /CSM/AF/AFM1-A/AFM123Q.Seq.d/                        620   e-176
SSL524 (SSL524Q) /CSM/SS/SSL5-A/SSL524Q.Seq.d/                        474   e-132
SSK651 (SSK651Q) /CSM/SS/SSK6-C/SSK651Q.Seq.d/                        161   3e-38
VHP778 (VHP778Q) /CSM/VH/VHP7-D/VHP778Q.Seq.d/                         42   0.021
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC115598|AC115598.2 Dictyostelium discoideum chromosome 2 map 581427-735498 strain AX4, complete sequence.   712   0.0    8
AB063522|AB063522.2 Wigglesworthia glossinidia endosymbiont of Glossina brevipalpis DNA, complete genome, section 2/2.    36   1e-16  29
AL844506|AL844506.1 Plasmodium falciparum chromosome  7.           38   9e-15  26
AE017263|AE017263.1 Mesoplasma florum L1 complete genome.          34   1e-14  33
CR382398|CR382398.1 Plasmodium falciparum chromosome 6, complete sequence; segment 1/5.    38   2e-14  23
BX842643|BX842643.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 2/4.    34   1e-13  28
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    38   2e-12  16
AC005140|AC005140.8 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    34   3e-12  27
CR382399|CR382399.1 Plasmodium falciparum chromosome 6, complete sequence; segment 2/5.    38   3e-12  26
AC117072|AC117072.3 Dictyostelium discoideum chromosome 2 map 3879572-4071762 strain AX4, complete sequence.    38   4e-12  14
dna update 2004. 9.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC115598_11(AC115598|pid:none) Dictyostelium discoideum chromoso...   495   e-138
AF063866_35(AF063866|pid:none) Melanoplus sanguinipes entomopoxv...    39   0.29
AJ251464_1(AJ251464|pid:none) Candida albicans eng1 gene for end...    36   2.5
CP000083_1966(CP000083|pid:none) Colwellia psychrerythraea 34H, ...    35   4.2
CP001080_1496(CP001080|pid:none) Sulfurihydrogenibium sp. YO3AOP...    35   5.5
EF217405_8(EF217405|pid:none) Shewanella hanedai ribosomal prote...    35   7.2
protein update 2008.11.24
PSORT 

 psg: 0.87  gvh: 0.35  alm: 0.28  top: 0.43  tms: 0.07  mit: 0.48  mip: 0.15
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.04  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: mitochondrial
	 24.0 %: endoplasmic reticulum
	 12.0 %: cytoplasmic
	 12.0 %: nuclear
	  8.0 %: Golgi
	  8.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for AFJ307 is mit
5' end seq. ID AFJ307F
5' end seq. 
>AFJ307F.Seq
CACTGTTGGCCTACTGGAACACTGTATATTGGTATGGGTGTTTTCAGAAAACACAACGAA
TTTATATACACCAAATAATTACAATGATTTCTTTATCAAAGAATTTTTCGAAGAATGGTT
ATTCTATAATCAAAAAGTATAATAAAATATCATCACCAAAAGCAATTATTGGAAATCCAT
CAATTTCCATAAAATCATCGTCATCATTTATTTTTAATATTAAAAATAACAACAACAACA
ACATTGGTACATCAAATACACTTAATATTAAAAGATTTTATAGTGAGGCTAATAATAAAA
ATAATAATGTTAATCAAGATAAAAAACAACAACAACAAAATTTTGATAATGATGATACTT
ATCATAATAAAGAAGAATTTACAAAACCACCAAAATATTTTTATGCTGTTGCATTATTAG
CTATTGGATGTATTGGAACTTATTTAGTAGATAATTTTATAAGTTTTAGTAATGAACAAA
TTTTAAGAGATATTAAAACAATTTTAAAGAGTGAAGATGCATTAGAAAGAAATAATGCAT
TGAAATCAATATCAACTAGAGCAGGCCAAGCATTTTGTGTTGAAAAAGTATTTAAATTAG
GTGTTG----------
Length of 5' end seq. 606
3' end seq. ID AFJ307Z
3' end seq. 
>AFJ307Z.Seq
----------GAAGCTGCTTCAAATTTATGGTTTCATTATTTTTTATCAAAAACTAATCA
AATTCAATTATTAACATCCAAATGATTTATTATATTGTTTGCAAAATTTAGCATATTCTG
ATAAATTTGAATTACAAAGAATATCAATTGCAACAGTAAAGATATTATTTGCAAATCGTG
AACTTTATCCAATGTTAAATAAATTGAAAGGAATGATATCACATTTAAATAAGAGTCAAG
ATGTTTTAATTTCAAATCATGCTGAATATTATTTAAATGTACTCAAAGGTGAATATATTC
CACCAGTTGGAAATATTTCTTTACCCGATGAAGAATTAGATCGTAAATTTAAAATTCATT
CATGTTATAATTTATTATTGGCACCATTAACTTATTTATATTGTTGTTCAAGATGGAAAA
AATATACAAGAGATCCAATTTATTTAAAAGCAAAGGGTATTTATGGTGGTACAATGATGT
TATTAGCAGCAGTTATTTCATCATTGACCCTTTTTCCAGATTATCATCCATCAAAGATTA
ATGCAAGATTAAAGAAAGAATTTTCATTAAATACAAAAACAATTGAAGAATTTGAAAGAT
GTAAAGCAATTTCATTAGCTGAAGCTGTTACTTTAGTNATAAAAATAAAAATAATAATAA
TAATAATAATAATAATAA
Length of 3' end seq. 668
Connected seq. ID AFJ307P
Connected seq. 
>AFJ307P.Seq
CACTGTTGGCCTACTGGAACACTGTATATTGGTATGGGTGTTTTCAGAAAACACAACGAA
TTTATATACACCAAATAATTACAATGATTTCTTTATCAAAGAATTTTTCGAAGAATGGTT
ATTCTATAATCAAAAAGTATAATAAAATATCATCACCAAAAGCAATTATTGGAAATCCAT
CAATTTCCATAAAATCATCGTCATCATTTATTTTTAATATTAAAAATAACAACAACAACA
ACATTGGTACATCAAATACACTTAATATTAAAAGATTTTATAGTGAGGCTAATAATAAAA
ATAATAATGTTAATCAAGATAAAAAACAACAACAACAAAATTTTGATAATGATGATACTT
ATCATAATAAAGAAGAATTTACAAAACCACCAAAATATTTTTATGCTGTTGCATTATTAG
CTATTGGATGTATTGGAACTTATTTAGTAGATAATTTTATAAGTTTTAGTAATGAACAAA
TTTTAAGAGATATTAAAACAATTTTAAAGAGTGAAGATGCATTAGAAAGAAATAATGCAT
TGAAATCAATATCAACTAGAGCAGGCCAAGCATTTTGTGTTGAAAAAGTATTTAAATTAG
GTGTTG----------GAAGCTGCTTCAAATTTATGGTTTCATTATTTTTTATCAAAAAC
TAATCAAATTCAATTATTAACATCCAAATGATTTATTATATTGTTTGCAAAATTTAGCAT
ATTCTGATAAATTTGAATTACAAAGAATATCAATTGCAACAGTAAAGATATTATTTGCAA
ATCGTGAACTTTATCCAATGTTAAATAAATTGAAAGGAATGATATCACATTTAAATAAGA
GTCAAGATGTTTTAATTTCAAATCATGCTGAATATTATTTAAATGTACTCAAAGGTGAAT
ATATTCCACCAGTTGGAAATATTTCTTTACCCGATGAAGAATTAGATCGTAAATTTAAAA
TTCATTCATGTTATAATTTATTATTGGCACCATTAACTTATTTATATTGTTGTTCAAGAT
GGAAAAAATATACAAGAGATCCAATTTATTTAAAAGCAAAGGGTATTTATGGTGGTACAA
TGATGTTATTAGCAGCAGTTATTTCATCATTGACCCTTTTTCCAGATTATCATCCATCAA
AGATTAATGCAAGATTAAAGAAAGAATTTTCATTAAATACAAAAACAATTGAAGAATTTG
AAAGATGTAAAGCAATTTCATTAGCTGAAGCTGTTACTTTAGTNATAAAAATAAAAATAA
TAATAATAATAATAATAATAATAA
Length of connected seq. 1274
Full length Seq ID -
Full length Seq. -
Length of full length seq. -