>SHE773 (SHE773Q) /CSM/SH/SHE7-D/SHE773Q.Seq.d/
CTGTTGGCCTACTGGNAAAATTTATTTTGTAATAAACTTAAAAAACACTACCACTATATT
TTCCCACGCTGTAAAAACAAAAAAACAAAAAACAAAAAAAATAAAAAGNAAAAAAAATCT
AAGAATCAAATACAAAACTTTAAAAACAATGTCACTCTTCAAGGAGAATTACAAATTTGT
TTGCTCCAAGTCAACAATCCAATTTAATTGAATGTAATGGTTATTTTTATCACTTTTATA
AAGGACAATGGGTTTGTTTATTTAATGATTGTGAATTAGTTATTCATAAATTTGAAAATT
ATAAATACCAAATTCAAGTTACAAGAATTGTAGATCAAGAACAAGAAAATGAAAGAAGAA
AACAAAGGGAAGCAGAAGGTTTAGAAGATGAAGAGGAAGAAGAGAATCTTGTAAATTATT
TTGAATTTCCAGTTACAAATGGATTAGAATTATATAATGAAATTGGACCATTAGGTAATT
TCTCATTAAGTTGGAAAGGTACAAATACAAGATTCTTATTCGAATTTGATAGTAATAATA
ATAATAACAATAATAACAATAATAATAATGAAAGTATTGAAACAACAGGGAAATCAATXX
XXXXXXXXAATACAAAAATGATGATGAAACTACCAATCAATTGTTTGTAGGCTACAATGG
TAATAATATGTTTTTAGTGGATCCCACGTGAATCAAAGAATAAAGTTACCGTTAAATTTC
ATGGTGGTTCAAATCCAAAATCTGTAACTACATGTGCTGCCACCACTGGTAGTGGTCAAA
TTGCTTTTGGTACGTCTAAAGGTGAAATCAAACTTTTCAGCAAAACTCAATTCGATCAAA
ATAAACGTTCCGTCACCTCTTCCGACCCTTTGGGTGCTATTGCTCGTTCACGTACTACCC
TACCGGGTATTGGTGATCCAATCGTTGGTATCGATGTCACCAAAGATGGTAAATGGATCG
TTGCAACCTGTAAACAATATATCATGGTCATACCAGCTTCACTCAAAGATGGTAGTAGTG
GTTTTGAAGATCGTTTGGGTGCTCGTCGTCCTTCACCAAAACGTTTAATTTTAAAACCTC
AAGATATTAAACGTATGGGTGGTGTCGTCAATTTCACACCAGCCAAATTCAATATCGTTG
GTGATGAACAATCTGAAACCTCCATTCTCACAAGCACCGGCAGTTTCCTTATCACTTGGA
ATTTTAGAAAAATTAAACAAAACATTTTGGACGTTTATCAAATTAAACAATTCCAAGATG
GTGTTGTAGCTGAACAATTTAAATCAAATAGAGATAATTCAATAGTTGTACTTTCCCNAT
AATGTAATTTTATCAAAAAAGAAATAATAACAAAATAATAAATAAA

vgllxkfil**t*ktlplyfptl*kqknkkqkk*kxkki*esntkl*KQCHSSRRITNLF
APSQQSNLIECNGYFYHFYKGQWVCLFNDCELVIHKFENYKYQIQVTRIVDQEQENERRK
QREAEGLEDEEEEENLVNYFEFPVTNGLELYNEIGPLGNFSLSWKGTNTRFLFEFDSNNN
NNNNNNNNNESIETTGKS---

---YKNDDETTNQLFVGYNGNNMFLVDPT*ike*syr*iswwfkskicnymcchhw*wsn
cfwyv*r*nqtfqqnsirsk*tfrhlfrpfgcycsftyyptgyw*snrwyrchqrw*mdr
cnl*tiyhghtsftqrw**wf*rsfgcsssftktfnfktsry*tygwcrqfhtsqiqyrw
**ti*nlhshkhrqfpyhlef*kn*tkhfgrlsn*tiprwccs*ti*ik*r*fnsctfpi
m*fyqkrnnnkiink

Frame A:
llaywxnlfcnklkkhyhyifprcknkktknkknkkxkksknqiqnfknnvtlqgelqic
llqvnnpi*lnvmvifitfikdngfvylmivn*lfinlkiintkfklqel*iknkkmkee
nkgkqkv*kmkrkkril*iilnfqlqmd*nyimkldh*vish*vgkvqiqdsysnlivii
iitiitiiimkvlkqqgnq---

---ntkmmmklpincl*atmviicf*wipresknkvtvkfhggsnpksvttcaattgsgq
iafgtskgeiklfsktqfdqnkrsvtssdplgaiarsrttlpgigdpivgidvtkdgkwi
vatckqyimvipaslkdgssgfedrlgarrpspkrlilkpqdikrmggvvnftpakfniv
gdeqsetsiltstgsflitwnfrkikqnildvyqikqfqdgvvaeqfksnrdnsivvlsx
*cnfikkeiitk**i

Frame B:
cwptgkiyfvinlkntttifshavktkkqktkkikxkknlrikyktlktmslfkenykfv
cskstiqfn*m*wlflsll*rtmglfi**l*isys*i*kl*ipnssykncrsrtrk*kkk
tkgsrrfrr*rgrrescklf*issykwirii**nwtir*fliklerykykiliri*****
**q**q****ky*nnrein---

---iqk***nyqsivcrlqw**yvfsgshvnqriklplnfmvvqiqnl*lhvlpplvvvk
lllvrlkvksnfsaklnsikinvpsplptlwvlllvhvlpyrvlviqslvsmspkmvngs
lqpvnniswsyqlhskmvvvvlkivwvlvvlhqnv*f*nlkilnvwvvssishqpnsisl
vmnnlkppfsqapavslslgileklnktfwtfiklnnskmvl*lnnlnqieiiq*lyfpx
nvilskkk**qnnk*

Frame C:
vgllxkfil**t*ktlplyfptl*kqknkkqkk*kxkki*esntkl*KQCHSSRRITNLF
APSQQSNLIECNGYFYHFYKGQWVCLFNDCELVIHKFENYKYQIQVTRIVDQEQENERRK
QREAEGLEDEEEEENLVNYFEFPVTNGLELYNEIGPLGNFSLSWKGTNTRFLFEFDSNNN
NNNNNNNNNESIETTGKS---

---YKNDDETTNQLFVGYNGNNMFLVDPT*ike*syr*iswwfkskicnymcchhw*wsn
cfwyv*r*nqtfqqnsirsk*tfrhlfrpfgcycsftyyptgyw*snrwyrchqrw*mdr
cnl*tiyhghtsftqrw**wf*rsfgcsssftktfnfktsry*tygwcrqfhtsqiqyrw
**ti*nlhshkhrqfpyhlef*kn*tkhfgrlsn*tiprwccs*ti*ik*r*fnsctfpi
m*fyqkrnnnkiink

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHE773 (SHE773Q) /CSM/SH/SHE7-D/SHE773Q.Seq.d/                       1449   0.0
AHH419 (AHH419Q) /CSM/AH/AHH4-A/AHH419Q.Seq.d/                       1380   0.0
SLB212 (SLB212Q) /CSM/SL/SLB2-A/SLB212Q.Seq.d/                       1364   0.0
CHM543 (CHM543Q) /CSM/CH/CHM5-B/CHM543Q.Seq.d/                       1332   0.0
AHA610 (AHA610Q) /CSM/AH/AHA6-A/AHA610Q.Seq.d/                       1324   0.0
SHB341 (SHB341Q) /CSM/SH/SHB3-B/SHB341Q.Seq.d/                       1310   0.0
AHH330 (AHH330Q) /CSM/AH/AHH3-B/AHH330Q.Seq.d/                       1310   0.0
AHD848 (AHD848Q) /CSM/AH/AHD8-B/AHD848Q.Seq.d/                       1298   0.0
AHN469 (AHN469Q) /CSM/AH/AHN4-C/AHN469Q.Seq.d/                       1291   0.0
SHB712 (SHB712Q) /CSM/SH/SHB7-A/SHB712Q.Seq.d/                       1289   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.  1241   0.0    3
AC068203|AC068203.2 Homo sapiens chromosome 4 clone RP11-683C4 map 4, WORKING DRAFT SEQUENCE, 18 unordered pieces.    52   0.034  1
AC104814|AC104814.5 Homo sapiens BAC clone RP11-683C4 from 4, complete sequence.    52   0.034  1
AC093790|AC093790.3 Homo sapiens BAC clone RP11-250F14 from 4, complete sequence.    52   0.034  1
CO731046|CO731046.1 JBW004G03.b_019.abi Pineapple green mature fruit cDNA library Ananas comosus cDNA clone JBW004G03 similar to unknown protein, mRNA sequence.    50   0.13   1
CO730778|CO730778.1 JBW001D09.b_073.abi Pineapple green mature fruit cDNA library Ananas comosus cDNA clone JBW001D09 similar to unknown protein, mRNA sequence.    50   0.13   1
AP008101|AP008101.1 Lotus corniculatus var. japonicus genomic DNA, clone:LjT22H07, TM1476.    50   0.13   1
AC142337|AC142337.1 Pan troglodytes chromosome 7 clone RP43-39F23, complete sequence.    38   0.37   7
BU018208|BU018208.1 QHE17P19.yg.ab1 QH_EFGHJ sunflower RHA280 Helianthus annuus cDNA clone QHE17P19, mRNA sequence.    48   0.53   1
CR493586|CR493586.1 mth2-167N22FM1 BAC end, cultivar Jemalong A17 of Medicago  truncatula.    48   0.53   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116986_49(AC116986|pid:none) Dictyostelium discoideum chromoso...   416   e-125
EF612188_1(EF612188|pid:none) Dictyostelium minutum unknown (Dm0...   305   3e-86
AE017345_303(AE017345|pid:none) Cryptococcus neoformans var. neo...   157   1e-36
(Q1MTR3) RecName: Full=Vacuolar import and degradation protein 2...   134   7e-30
CU633457_256(CU633457|pid:none) Podospora anserina genomic DNA c...   130   1e-28
FM992693_11(FM992693|pid:none) Candida dubliniensis CD36 chromos...   129   2e-28
CR382130_222(CR382130|pid:none) Yarrowia lipolytica strain CLIB1...   129   2e-28
AM920435_379(AM920435|pid:none) Penicillium chrysogenum Wisconsi...   126   1e-27
AP007151_1191(AP007151|pid:none) Aspergillus oryzae RIB40 genomi...   126   2e-27
CR382135_411(CR382135|pid:none) Debaryomyces hansenii strain CBS...   116   2e-24

 psg: 0.84  gvh: 0.41  alm: 0.51  top: 0.53  tms: 0.00  mit: 0.45  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: mitochondrial
	 20.0 %: nuclear
	 16.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for SHE773 is mit

>SHE773F.Seq
CTGTTGGCCTACTGGNAAAATTTATTTTGTAATAAACTTAAAAAACACTACCACTATATT
TTCCCACGCTGTAAAAACAAAAAAACAAAAAACAAAAAAAATAAAAAGNAAAAAAAATCT
AAGAATCAAATACAAAACTTTAAAAACAATGTCACTCTTCAAGGAGAATTACAAATTTGT
TTGCTCCAAGTCAACAATCCAATTTAATTGAATGTAATGGTTATTTTTATCACTTTTATA
AAGGACAATGGGTTTGTTTATTTAATGATTGTGAATTAGTTATTCATAAATTTGAAAATT
ATAAATACCAAATTCAAGTTACAAGAATTGTAGATCAAGAACAAGAAAATGAAAGAAGAA
AACAAAGGGAAGCAGAAGGTTTAGAAGATGAAGAGGAAGAAGAGAATCTTGTAAATTATT
TTGAATTTCCAGTTACAAATGGATTAGAATTATATAATGAAATTGGACCATTAGGTAATT
TCTCATTAAGTTGGAAAGGTACAAATACAAGATTCTTATTCGAATTTGATAGTAATAATA
ATAATAACAATAATAACAATAATAATAATGAAAGTATTGAAACAACAGGGAAATCAATNN
NNNNNNNN

>SHE773Z.Seq
NNNNNNNNNNAATACAAAAATGATGATGAAACTACCAATCAATTGTTTGTAGGCTACAAT
GGTAATAATATGTTTTTAGTGGATCCCACGTGAATCAAAGAATAAAGTTACCGTTAAATT
TCATGGTGGTTCAAATCCAAAATCTGTAACTACATGTGCTGCCACCACTGGTAGTGGTCA
AATTGCTTTTGGTACGTCTAAAGGTGAAATCAAACTTTTCAGCAAAACTCAATTCGATCA
AAATAAACGTTCCGTCACCTCTTCCGACCCTTTGGGTGCTATTGCTCGTTCACGTACTAC
CCTACCGGGTATTGGTGATCCAATCGTTGGTATCGATGTCACCAAAGATGGTAAATGGAT
CGTTGCAACCTGTAAACAATATATCATGGTCATACCAGCTTCACTCAAAGATGGTAGTAG
TGGTTTTGAAGATCGTTTGGGTGCTCGTCGTCCTTCACCAAAACGTTTAATTTTAAAACC
TCAAGATATTAAACGTATGGGTGGTGTCGTCAATTTCACACCAGCCAAATTCAATATCGT
TGGTGATGAACAATCTGAAACCTCCATTCTCACAAGCACCGGCAGTTTCCTTATCACTTG
GAATTTTAGAAAAATTAAACAAAACATTTTGGACGTTTATCAAATTAAACAATTCCAAGA
TGGTGTTGTAGCTGAACAATTTAAATCAAATAGAGATAATTCAATAGTTGTACTTTCCCN
ATAATGTAATTTTATCAAAAAAGAAATAATAACAAAATAATAAATAAA

>SHE773P.Seq
CTGTTGGCCTACTGGNAAAATTTATTTTGTAATAAACTTAAAAAACACTACCACTATATT
TTCCCACGCTGTAAAAACAAAAAAACAAAAAACAAAAAAAATAAAAAGNAAAAAAAATCT
AAGAATCAAATACAAAACTTTAAAAACAATGTCACTCTTCAAGGAGAATTACAAATTTGT
TTGCTCCAAGTCAACAATCCAATTTAATTGAATGTAATGGTTATTTTTATCACTTTTATA
AAGGACAATGGGTTTGTTTATTTAATGATTGTGAATTAGTTATTCATAAATTTGAAAATT
ATAAATACCAAATTCAAGTTACAAGAATTGTAGATCAAGAACAAGAAAATGAAAGAAGAA
AACAAAGGGAAGCAGAAGGTTTAGAAGATGAAGAGGAAGAAGAGAATCTTGTAAATTATT
TTGAATTTCCAGTTACAAATGGATTAGAATTATATAATGAAATTGGACCATTAGGTAATT
TCTCATTAAGTTGGAAAGGTACAAATACAAGATTCTTATTCGAATTTGATAGTAATAATA
ATAATAACAATAATAACAATAATAATAATGAAAGTATTGAAACAACAGGGAAATCAAT--
--------AATACAAAAATGATGATGAAACTACCAATCAATTGTTTGTAGGCTACAATGG
TAATAATATGTTTTTAGTGGATCCCACGTGAATCAAAGAATAAAGTTACCGTTAAATTTC
ATGGTGGTTCAAATCCAAAATCTGTAACTACATGTGCTGCCACCACTGGTAGTGGTCAAA
TTGCTTTTGGTACGTCTAAAGGTGAAATCAAACTTTTCAGCAAAACTCAATTCGATCAAA
ATAAACGTTCCGTCACCTCTTCCGACCCTTTGGGTGCTATTGCTCGTTCACGTACTACCC
TACCGGGTATTGGTGATCCAATCGTTGGTATCGATGTCACCAAAGATGGTAAATGGATCG
TTGCAACCTGTAAACAATATATCATGGTCATACCAGCTTCACTCAAAGATGGTAGTAGTG
GTTTTGAAGATCGTTTGGGTGCTCGTCGTCCTTCACCAAAACGTTTAATTTTAAAACCTC
AAGATATTAAACGTATGGGTGGTGTCGTCAATTTCACACCAGCCAAATTCAATATCGTTG
GTGATGAACAATCTGAAACCTCCATTCTCACAAGCACCGGCAGTTTCCTTATCACTTGGA
ATTTTAGAAAAATTAAACAAAACATTTTGGACGTTTATCAAATTAAACAATTCCAAGATG
GTGTTGTAGCTGAACAATTTAAATCAAATAGAGATAATTCAATAGTTGTACTTTCCCNAT
AATGTAATTTTATCAAAAAAGAAATAATAACAAAATAATAAATAAA