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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHE753
Library SH
(Link to library)
Clone ID SHE753
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11302-1
Original site URL
Representative seq. ID SHE753P
(Link to Original site)
Representative DNA sequence 
>SHE753 (SHE753Q) /CSM/SH/SHE7-C/SHE753Q.Seq.d/
ACTGTTGGCCTACTGGNACAAATTTGGATGTTAAATTAATTATAATTTGTTAATATAACA
TTTTGTATATATATATATATAAAAAATAAAAAACTCAAATGACAGGATTTTCAATTCCTA
ATATGAGTATGTTACTCAAATTAGCAAATAGTAGATTGGTATTACATAAAAATAAAAATT
TTGAACAAAGTAATGTTCATAAAAAAGAGATTGCAGAATTATTACAATTAGGTAAAGAAG
AACAAGCAAGAGTTAGAACGGTTTCAGTTATTAATGAAGATTATCACACTGAAGTTTTAG
GTATTTTAATTATTTATTGTGAAACTTTAGCAAATAGAATTAGAGGAATTGAAGGTGTAA
AGGTATGCCCACCAGATTTAAAAGAAGCATGTTGTAGTATTATATTTGCATCACCATATT
TAGATAAACAAGTTGAATTATATAAGATTAGAAAGAGATTAATTGAAAAATTTGGTAAAA
AATTCCCAGAGGAATGTATAGATTGTTGTTGTATCAATCCAAAGATTGTACATAGATTAT
CAAATAXXXXXXXXXXTGGATTGTGGTGATGGTGTTTGTCATACCGTGCCAATCTATGAA
GGTTATTCTATTCCACATGCAATTAAAAGAATCGATATCGGTGGTCGTGATATCACAGAG
TATTTAATGCGTTTACTCACTGAAAGAGGTTATGCTTTCACTACAACCGCCGAAAGAGAA
ATCGTACGTGATATCAAAGAGAAAACTTCATTTGTCTCTCAAGATTTCTATGCCTCTATG
AATGCTCCAGAGGATAATAATATACTCAAGGATTATACGATGCCCGATAAACAAGTTTTA
ACCATTGGTAAAGAAAGATTCCGTTGTTATGAAGCTTTCTTTGACCCTTCAGTACTAGGT
AAAGATCAAAATGGTATTCATCACCTCTTAAATGAAACTATCACCTCTTGTGATATTGAT
ATTCGTCGTGATCTCTATAAAAATATTGTAGTTTCTGGTGGTTCAACAATGGCAACAGGT
TTTGAAAAACGTTTACAAAAAGAAATGGAACTTTTAGTTTCAAATCCTCAAGTCCAAATT
AAAATTAATGCACCACCAAATCGTGATATTAGTGTTTGGTGTGGTGGTTCGGTTTTAGGT
GATCTTAAAACTTTTTCGGATCAATGGATTACAAAACAAGAATATGAGAAGTGGTAGAGG
AATTGTTCATAGAAAAGTTTTAAAAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
cwptgtnldvkliiic*ynilyiyiykk*KTQMTGFSIPNMSMLLKLANSRLVLHKNKNF
EQSNVHKKEIAELLQLGKEEQARVRTVSVINEDYHTEVLGILIIYCETLANRIRGIEGVK
VCPPDLKEACCSIIFASPYLDKQVELYKIRKRLIEKFGKKFPEECIDCCCINPKIVHRLS
N---

---DCGDGVCHTVPIYEGYSIPHAIKRIDIGGRDITEYLMRLLTERGYAFTTTAEREIVR
DIKEKTSFVSQDFYASMNAPEDNNILKDYTMPDKQVLTIGKERFRCYEAFFDPSVLGKDQ
NGIHHLLNETITSCDIDIRRDLYKNIVVSGGSTMATGFEKRLQKEMELLVSNPQVQIKIN
APPNRDISVWCGGSVLGDLKTFSDQWITKQEYEKW*rncs*ksfkk
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllxqiwmln*l*fvnitfciyiyiknkklk*qdfqfli*vcysn*qivdwyyikiki
lnkvmfikkrlqnyyn*vkknkqelerfqllmkiitlkf*vf*lfivkl*qieleelkv*
ryahqi*kkhvvvlylhhhi*inklnyirlerd*lknlvknsqrnv*ivvvsiqrlyidy
qi---

---wivvmvfvipcqsmkvilfhmqlkesisvvvisqsi*cvyslkevmlslqppkeksy
viskrklhlslkismpl*mlqriiiysriircpinkf*plvkkdsvvmklsltlqy*vki
kmvfits*mklsplvilifvvisikil*flvvqqwqqvlknvykkkwnf*fqilksklkl
mhhqivilvfgvvvrf*vilklfringlqnknmrsgrgivhrkvlkk

Frame B:
llaywxkfgc*inynlli*hfvyiyi*kiknsndrifns*yeyvtqisk**igit*k*kf
*tk*cs*krdcriitir*rrtsks*ngfsy**rlsh*sfryfnyll*nfsk*n*rn*rck
gmptrfkrsml*yyicitifr*ts*ii*d*kein*kiw*kiprgmyrlllyqskdct*ii
k---

---glw*wclsyranl*rlfystcn*knryrws*yhrvfnafth*krlcfhynrrkrnrt
*yqrenficlsrflclyecsrg**ytqglydar*tsfnhw*rkipll*sfl*pfstr*rs
kwyssplk*nyhll*y*yss*sl*kycsfwwfnngnrf*ktftkrngtfsfkssspn*n*
cttks*y*clvwwfgfr*s*nffgsmdyktri*evveelfiekf*kk

Frame C:
cwptgtnldvkliiic*ynilyiyiykk*KTQMTGFSIPNMSMLLKLANSRLVLHKNKNF
EQSNVHKKEIAELLQLGKEEQARVRTVSVINEDYHTEVLGILIIYCETLANRIRGIEGVK
VCPPDLKEACCSIIFASPYLDKQVELYKIRKRLIEKFGKKFPEECIDCCCINPKIVHRLS
N---

---DCGDGVCHTVPIYEGYSIPHAIKRIDIGGRDITEYLMRLLTERGYAFTTTAEREIVR
DIKEKTSFVSQDFYASMNAPEDNNILKDYTMPDKQVLTIGKERFRCYEAFFDPSVLGKDQ
NGIHHLLNETITSCDIDIRRDLYKNIVVSGGSTMATGFEKRLQKEMELLVSNPQVQIKIN
APPNRDISVWCGGSVLGDLKTFSDQWITKQEYEKW*rncs*ksfkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHE753 (SHE753Q) /CSM/SH/SHE7-C/SHE753Q.Seq.d/                       2177   0.0
AHE553 (AHE553Q) /CSM/AH/AHE5-C/AHE553Q.Seq.d/                       1304   0.0
AHI741 (AHI741Q) /CSM/AH/AHI7-B/AHI741Q.Seq.d/                       1302   0.0
AHM227 (AHM227Q) /CSM/AH/AHM2-B/AHM227Q.Seq.d/                       1294   0.0
SHE313 (SHE313Q) /CSM/SH/SHE3-A/SHE313Q.Seq.d/                       1292   0.0
VHA813 (VHA813Q) /CSM/VH/VHA8-A/VHA813Q.Seq.d/                       1289   0.0
VHB304 (VHB304Q) /CSM/VH/VHB3-A/VHB304Q.Seq.d/                       1287   0.0
VHD820 (VHD820Q) /CSM/VH/VHD8-A/VHD820Q.Seq.d/                       1281   0.0
SHC311 (SHC311Q) /CSM/SH/SHC3-A/SHC311Q.Seq.d/                       1281   0.0
AHL385 (AHL385Q) /CSM/AH/AHL3-D/AHL385Q.Seq.d/                       1281   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AY763999|AY763999.1 Marginopora vertebralis clone MvA1250_40 actin type 2 gene, partial cds.    52   1e-16  4
AY763998|AY763998.1 Marginopora vertebralis clone MvA1250_37 actin type 2 gene, partial cds.    52   1e-16  4
AY763997|AY763997.1 Marginopora vertebralis clone MvA1247_25 actin type 2 gene, partial cds.    52   1e-16  4
X52604|X52604.1 Artemia mRNA for actin (clone pArAct302).          66   3e-15  3
AJ269586|AJ269586.1 Artemia franciscana mRNA for actin (actin 302 gene), clone RT-PCR-14.    66   4e-15  3
AJ269585|AJ269585.1 Artemia franciscana mRNA for actin (actin 302 gene), clone RT-PCR-7.    66   4e-15  3
AJ269584|AJ269584.1 Artemia franciscana mRNA for actin (actin 302 gene), clone RT-PCR-6.    66   4e-15  3
AJ269583|AJ269583.1 Artemia franciscana mRNA for actin (actin 302 gene), clone RT-PCR-3.    66   4e-15  3
AJ269581|AJ269581.1 Artemia franciscana mRNA for actin (actin 302 gene), clone RT-PCR-18.    66   4e-15  3
AJ269580|AJ269580.1 Artemia franciscana mRNA for actin (actin 302 gene), clone RT-PCR-13.    66   4e-15  3
dna update 2006. 3. 8
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AJ132370_1(AJ132370|pid:none) Allogromia sp. mRNA for actin 1.        270   6e-71
AY452189_1(AY452189|pid:none) Sorosphaera veronicae actin 1 gene...   262   2e-68
AY452190_1(AY452190|pid:none) Sorosphaera veronicae actin 2 gene...   262   2e-68
AF363539_1(AF363539|pid:none) Cercomonas sp. ATCC 50319 actin (a...   261   4e-68
DQ980435_1(DQ980435|pid:none) Thaumatomonas sp. TMT002 actin gen...   261   4e-68
AF363536_1(AF363536|pid:none) Cercomonas sp. ATCC 50318 actin-1 ...   260   6e-68
AY764017_1(AY764017|pid:none) Sorites sp. isolate X-1646 clone S...   260   8e-68
(P11426) RecName: Full=Actin;  &ATAXE(A29877;A54526;S33463) &M16...   260   8e-68
EU334881_1(EU334881|pid:none) Andalucia incarcerata isolate MB1 ...   259   1e-67
AM411663_1(AM411663|pid:none) Plasmodiophora brassicae actII gen...   259   2e-67
protein update 2009. 4.17
PSORT 

 psg: 0.87  gvh: 0.48  alm: 0.41  top: 0.70  tms: 0.00  mit: 0.33  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: mitochondrial
	 32.0 %: nuclear
	 28.0 %: cytoplasmic
	  4.0 %: peroxisomal

>> prediction for SHE753 is mit
5' end seq. ID SHE753F
5' end seq. 
>SHE753F.Seq
ACTGTTGGCCTACTGGNACAAATTTGGATGTTAAATTAATTATAATTTGTTAATATAACA
TTTTGTATATATATATATATAAAAAATAAAAAACTCAAATGACAGGATTTTCAATTCCTA
ATATGAGTATGTTACTCAAATTAGCAAATAGTAGATTGGTATTACATAAAAATAAAAATT
TTGAACAAAGTAATGTTCATAAAAAAGAGATTGCAGAATTATTACAATTAGGTAAAGAAG
AACAAGCAAGAGTTAGAACGGTTTCAGTTATTAATGAAGATTATCACACTGAAGTTTTAG
GTATTTTAATTATTTATTGTGAAACTTTAGCAAATAGAATTAGAGGAATTGAAGGTGTAA
AGGTATGCCCACCAGATTTAAAAGAAGCATGTTGTAGTATTATATTTGCATCACCATATT
TAGATAAACAAGTTGAATTATATAAGATTAGAAAGAGATTAATTGAAAAATTTGGTAAAA
AATTCCCAGAGGAATGTATAGATTGTTGTTGTATCAATCCAAAGATTGTACATAGATTAT
CAAATANNNNNNNNNN
Length of 5' end seq. 556
3' end seq. ID SHE753Z
3' end seq. 
>SHE753Z.Seq
NNNNNNNNNNTGGATTGTGGTGATGGTGTTTGTCATACCGTGCCAATCTATGAAGGTTAT
TCTATTCCACATGCAATTAAAAGAATCGATATCGGTGGTCGTGATATCACAGAGTATTTA
ATGCGTTTACTCACTGAAAGAGGTTATGCTTTCACTACAACCGCCGAAAGAGAAATCGTA
CGTGATATCAAAGAGAAAACTTCATTTGTCTCTCAAGATTTCTATGCCTCTATGAATGCT
CCAGAGGATAATAATATACTCAAGGATTATACGATGCCCGATAAACAAGTTTTAACCATT
GGTAAAGAAAGATTCCGTTGTTATGAAGCTTTCTTTGACCCTTCAGTACTAGGTAAAGAT
CAAAATGGTATTCATCACCTCTTAAATGAAACTATCACCTCTTGTGATATTGATATTCGT
CGTGATCTCTATAAAAATATTGTAGTTTCTGGTGGTTCAACAATGGCAACAGGTTTTGAA
AAACGTTTACAAAAAGAAATGGAACTTTTAGTTTCAAATCCTCAAGTCCAAATTAAAATT
AATGCACCACCAAATCGTGATATTAGTGTTTGGTGTGGTGGTTCGGTTTTAGGTGATCTT
AAAACTTTTTCGGATCAATGGATTACAAAACAAGAATATGAGAAGTGGTAGAGGAATTGT
TCATAGAAAAGTTTTAAAAAAAA
Length of 3' end seq. 683
Connected seq. ID SHE753P
Connected seq. 
>SHE753P.Seq
ACTGTTGGCCTACTGGNACAAATTTGGATGTTAAATTAATTATAATTTGTTAATATAACA
TTTTGTATATATATATATATAAAAAATAAAAAACTCAAATGACAGGATTTTCAATTCCTA
ATATGAGTATGTTACTCAAATTAGCAAATAGTAGATTGGTATTACATAAAAATAAAAATT
TTGAACAAAGTAATGTTCATAAAAAAGAGATTGCAGAATTATTACAATTAGGTAAAGAAG
AACAAGCAAGAGTTAGAACGGTTTCAGTTATTAATGAAGATTATCACACTGAAGTTTTAG
GTATTTTAATTATTTATTGTGAAACTTTAGCAAATAGAATTAGAGGAATTGAAGGTGTAA
AGGTATGCCCACCAGATTTAAAAGAAGCATGTTGTAGTATTATATTTGCATCACCATATT
TAGATAAACAAGTTGAATTATATAAGATTAGAAAGAGATTAATTGAAAAATTTGGTAAAA
AATTCCCAGAGGAATGTATAGATTGTTGTTGTATCAATCCAAAGATTGTACATAGATTAT
CAAATA----------TGGATTGTGGTGATGGTGTTTGTCATACCGTGCCAATCTATGAA
GGTTATTCTATTCCACATGCAATTAAAAGAATCGATATCGGTGGTCGTGATATCACAGAG
TATTTAATGCGTTTACTCACTGAAAGAGGTTATGCTTTCACTACAACCGCCGAAAGAGAA
ATCGTACGTGATATCAAAGAGAAAACTTCATTTGTCTCTCAAGATTTCTATGCCTCTATG
AATGCTCCAGAGGATAATAATATACTCAAGGATTATACGATGCCCGATAAACAAGTTTTA
ACCATTGGTAAAGAAAGATTCCGTTGTTATGAAGCTTTCTTTGACCCTTCAGTACTAGGT
AAAGATCAAAATGGTATTCATCACCTCTTAAATGAAACTATCACCTCTTGTGATATTGAT
ATTCGTCGTGATCTCTATAAAAATATTGTAGTTTCTGGTGGTTCAACAATGGCAACAGGT
TTTGAAAAACGTTTACAAAAAGAAATGGAACTTTTAGTTTCAAATCCTCAAGTCCAAATT
AAAATTAATGCACCACCAAATCGTGATATTAGTGTTTGGTGTGGTGGTTCGGTTTTAGGT
GATCTTAAAACTTTTTCGGATCAATGGATTACAAAACAAGAATATGAGAAGTGGTAGAGG
AATTGTTCATAGAAAAGTTTTAAAAAAAA
Length of connected seq. 1219
Full length Seq ID -
Full length Seq. -
Length of full length seq. -