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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHE454
Library SH
(Link to library)
Clone ID SHE454
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11926-1
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>SHE454 (SHE454Q) /CSM/SH/SHE4-C/SHE454Q.Seq.d/
TACTGATGATAACAATGCCTGTACACTTGATTCCTGTTCACCATCAACTGGTGTTTCCCA
CACCCCAATTAACTGTGATGATAGTAATCCATGTACCGTAGACTCATGTTCAAATTCAAC
CGGTTGTGTAAACACTCCAGTCAATGTTGATGATAATAATCCATGTACTGTAGATGCTTG
TACCAAATCAACAGGTGTCACACATACCCCAGTAAATGTAGATGATAACAACAAATGTAC
AATTGATGCATGTACCAAAGAAGGTGGTGTAACTCATACTCCAGTCAACACTGATGATAA
CAATGCCTGTACAATTGATTCTTGTTCACCATCAACCGGTATTTCCCACACCCCAATCAA
TTGTGATGATAAAAAGGCCTGTACAGTTGATTCATGTTCAAATTCAACTGGTTGTGTAAA
TACTCCAATTTCCTGTGACGATAATAATCCATGTACTGTTGATTCTTGCGATGACTTAAC
TGGTTGTTGCAATACTCCAATCAATGTTGATGATAATAATCCATGTACCATCGATGCCTG
TACTAAATCAACAGGTGTTACTCATACCCCAGTCAATGTNGATGATAATAACAAATGTAC
AATTGATACATGTACCAAAGAAGGTGGTGTAACTCATACTCCAATCAATACTGATGATAA
CAATGCCTGTACCCTTGATTCCTGCTCACNATNANCTGGTGTTTCCCATACCCCCAATAA
ACNGTGATGATAATAATANATGGACCTGTAGAATTCATGTTCCAAACTCAACTGGTTGNG
GTAAATACCTCCAATTAACTGGGACGATANTAATNCNTGTNCCXXXXXXXXXX
sequence update 2002.10.25
Translated Amino Acid sequence 
TDDNNACTLDSCSPSTGVSHTPINCDDSNPCTVDSCSNSTGCVNTPVNVDDNNPCTVDAC
TKSTGVTHTPVNVDDNNKCTIDACTKEGGVTHTPVNTDDNNACTIDSCSPSTGISHTPIN
CDDKKACTVDSCSNSTGCVNTPISCDDNNPCTVDSCDDLTGCCNTPINVDDNNPCTIDAC
TKSTGVTHTPVNVDDNNKCTIDTCTKEGGVTHTPINTDDNNACTLDSCSXXXGVSHTPNK
X****xmdl*nscsklnwlx*ippinwddxnxc---
Translated Amino Acid sequence (All Frames) 
Frame A:
y***qclyt*flftinwcfphpn*l****smyrrlmfkfnrlckhssqc****smycrcl
yqinrchtypskcr**qqmyn*cmyqrrwcnsyssqh***qclyn*flftinryfphpnq
l***kglys*fmfkfnwlckysnfl*r**smyc*flr*lnwllqysnqc****smyhrcl
y*inrcysypsqcx***qmyn*ymyqrrwcnsysnqy***qclyp*flltxxwcfpypq*
tvmiiixgpvefmfqtqlvxvntsn*lgrx*xxx---


Frame B:
TDDNNACTLDSCSPSTGVSHTPINCDDSNPCTVDSCSNSTGCVNTPVNVDDNNPCTVDAC
TKSTGVTHTPVNVDDNNKCTIDACTKEGGVTHTPVNTDDNNACTIDSCSPSTGISHTPIN
CDDKKACTVDSCSNSTGCVNTPISCDDNNPCTVDSCDDLTGCCNTPINVDDNNPCTIDAC
TKSTGVTHTPVNVDDNNKCTIDTCTKEGGVTHTPINTDDNNACTLDSCSXXXGVSHTPNK
X****xmdl*nscsklnwlx*ippinwddxnxc---


Frame C:
lmitmpvhlipvhhqlvfptpqltvmivihvp*thvqiqpvv*tlqsmlmiiihvl*mlv
pnqqvshipq*m*mittnvqlmhvpkkvv*lilqstlmitmpvqlilvhhqpvfptpqsi
vmikrpvqlihvqiqlvv*ilqfpvtiiihvllilamt*lvvailqsmlmiiihvpsmpv
lnqqvllipqsmxmiitnvqlihvpkkvv*lilqsilmitmpvplipahxxlvfpippin
xddnnxwtcrihvpnstgxgkylqltgtixxxv---
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHE454 (SHE454Q) /CSM/SH/SHE4-C/SHE454Q.Seq.d/                       1566   0.0
SHJ336 (SHJ336Q) /CSM/SH/SHJ3-B/SHJ336Q.Seq.d/                       1338   0.0
SHG431 (SHG431Q) /CSM/SH/SHG4-B/SHG431Q.Seq.d/                       1247   0.0
CHS280 (CHS280Q) /CSM/CH/CHS2-D/CHS280Q.Seq.d/                       1207   0.0
SHK455 (SHK455Q) /CSM/SH/SHK4-C/SHK455Q.Seq.d/                       1203   0.0
SHG408 (SHG408Q) /CSM/SH/SHG4-A/SHG408Q.Seq.d/                       1164   0.0
SHA257 (SHA257Q) /CSM/SH/SHA2-C/SHA257Q.Seq.d/                       1132   0.0
SHA221 (SHA221Q) /CSM/SH/SHA2-A/SHA221Q.Seq.d/                       1017   0.0
SHD894 (SHD894Q) /CSM/SH/SHD8-D/SHD894Q.Seq.d/                        981   0.0
SHC394 (SHC394Q) /CSM/SH/SHC3-D/SHC394Q.Seq.d/                        975   0.0
own update 2002.12. 5
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X78948|X78948.1 D.minutum ecmB gene.                               88   3e-32  5
CK991559|CK991559.1 EST0111 Eyestalk cDNA library Penaeus monodon cDNA clone ES-N-S03-555-W 5' similar to Histidine-rich glycoprotein precursor (Plasmodium lophurae), mRNA sequence.    30   0.031  4
BQ739629|BQ739629.1 PfESToab48c05.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to TR:Q9U0J6 Q9U0J6 HYPOTHETICAL 312.5 KD PROTEIN. ;, mRNA sequence.    30   0.19   4
BX231032|BX231032.1 Danio rerio genomic clone DKEY-254O18, genomic survey sequence.    30   0.21   4
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    38   0.45   9
AF134686|AF134686.1 Plasmodium falciparum strain IVC10 from Cote d' Ivoire CG2 omega repeat (cg2) gene, partial cds.    28   0.56   4
DR453650|DR453650.1 CM015E04 Cotton Lambda Zap Express Library Gossypium hirsutum cDNA, mRNA sequence.    32   2.5    3
AC117072|AC117072.3 Dictyostelium discoideum chromosome 2 map 3879572-4071762 strain AX4, complete sequence.    38   2.8    6
AF500784|AF500784.1 Piromyces sp. E2 clone lambda11 beta-glucosidase Cel1C gene, complete cds.    44   3.1    2
G37776|G37776.1 atp Plasmodium falciparum haploid Plasmodium falciparum STS genomic, sequence tagged site.    28   3.2    3
dna update 2006. 2.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A27020(A27020)DIF-induced prestalk pDd63 protein precursor - sli...   373   e-102
(P11976) RecName: Full=Prestalk protein; AltName: Full=Extracell...   367   e-100
A26838(A26838)prestalk protein precursor - slime mold (Dictyoste...   367   e-100
S44208(S44208) extracellular matrix protein B - Dictyostelium mi...   312   7e-84
AC117072_61(AC117072|pid:none) Dictyostelium discoideum chromoso...   277   3e-73
AC116984_103(AC116984|pid:none) Dictyostelium discoideum chromos...   275   1e-72
(Q54G85) RecName: Full=Protein psiJ; Flags: Precursor;                127   6e-28
(Q54CH8) RecName: Full=Protein psiN; Flags: Precursor;                126   7e-28
(Q54C31) RecName: Full=Protein psiR; Flags: Precursor;                119   1e-25
(Q54C32) RecName: Full=Protein psiQ; Flags: Precursor;                108   3e-22
protein update 2009. 4.17
PSORT 

 psg: 0.75  gvh: 0.48  alm: 0.57  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 32.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal

>> prediction for SHE454 is nuc
5' end seq. ID SHE454F
5' end seq. 
>SHE454F.Seq
TACTGATGATAACAATGCCTGTACACTTGATTCCTGTTCACCATCAACTGGTGTTTCCCA
CACCCCAATTAACTGTGATGATAGTAATCCATGTACCGTAGACTCATGTTCAAATTCAAC
CGGTTGTGTAAACACTCCAGTCAATGTTGATGATAATAATCCATGTACTGTAGATGCTTG
TACCAAATCAACAGGTGTCACACATACCCCAGTAAATGTAGATGATAACAACAAATGTAC
AATTGATGCATGTACCAAAGAAGGTGGTGTAACTCATACTCCAGTCAACACTGATGATAA
CAATGCCTGTACAATTGATTCTTGTTCACCATCAACCGGTATTTCCCACACCCCAATCAA
TTGTGATGATAAAAAGGCCTGTACAGTTGATTCATGTTCAAATTCAACTGGTTGTGTAAA
TACTCCAATTTCCTGTGACGATAATAATCCATGTACTGTTGATTCTTGCGATGACTTAAC
TGGTTGTTGCAATACTCCAATCAATGTTGATGATAATAATCCATGTACCATCGATGCCTG
TACTAAATCAACAGGTGTTACTCATACCCCAGTCAATGTNGATGATAATAACAAATGTAC
AATTGATACATGTACCAAAGAAGGTGGTGTAACTCATACTCCAATCAATACTGATGATAA
CAATGCCTGTACCCTTGATTCCTGCTCACNATNANCTGGTGTTTCCCATACCCCCAATAA
ACNGTGATGATAATAATANATGGACCTGTAGAATTCATGTTCCAAACTCAACTGGTTGNG
GTAAATACCTCCAATTAACTGGGACGATANTAATNCNTGTNCCNNNNNNNNNN
Length of 5' end seq. 833
3' end seq. ID -
3' end seq. -
Length of 3' end seq. -
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -