>SHE452 (SHE452Q) /CSM/SH/SHE4-C/SHE452Q.Seq.d/
CTATAATAAAAAACGAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAAT
GGTCAAAATGAAGAATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGA
GAGCAACAATTAACTAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAG
ATAGAAGAAGCATTAGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTT
TCAATTTATAATAAACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCAT
AAAATTTTCAATAAAGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTA
AAAGAAACAGCAGAAAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGT
GAATCATCAGAAGGTGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGAT
TTATCAGCATTTGTAAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCA
TCGATCTATCAATCACAAAAGAATATACATACACAATTCTTTAAGAATGTTCATTTACAA
GCTGTCTATTATTCAATGATTGATATATTTTCAGTATTAATAAATTTAGATAGTATAATT
ACACAAAATACTGCTTTAXXXXXXXXXXGAATAATATGTACCNTTCAGAGTTGGTGACAG
AGTTGGAAAGAGACATTAGACAATTGGGTGTCTCCGAGACTGGTTTAACCTTTTTAGATC
ATTTTAGACTTTTAATCACTCATATTGGTAATGCTATGGGTTATATTAGATTGGTCAGAT
CCGGTGGTTTACACTATTGTAGTAATGCTATCAAATTCGTACCAGACTTGAAAAAGATTC
CAAAATTCCAAGATTTAACCTCAAAAGATGCCCTCTCACCTGAAACAATTCAAGCTAGTA
CCAATTTGGATTCAATCATTCATAATCTTTCAAATAATTTATCAGAGGGTACTGAATACT
TTAAGATGTTGGTAAACGTTTTCGCTACAGAGTTTCGAAATATCGCTAATCAACATTTAA
AGAATTTCTATATCATCGTACCTGCCTTGGCCTCAAATTACATCGATCATATGATCAATA
GTAAAGATAAACTCTTTAAGAAATCAAAAGCAATCGGTGCTGAAGCTTTATTATTCACTG
ATGATGGCTTTGCAATTGGTTTGGCTTATATCTTAAAATTATTAGATCAAAATAAAGATT
TCGATTCTCTTCATTGGTTCGATCGTATTCAATTGAAATGTGAAACNGATCAAAAGAGAA
TGTTGGCTGAAGCAAATGCAAAAGGTATCAAAGAGGATCAAAATCATTTAGCAATTAAAA
AGATTCAAAATTTCCAACAAGAATAGAATATTNAAATTCTTTCTC

l**ktnnpi*KMATSYSANNGQNEEYTFDAFEDGSSKLVGEQQLTKLNQFAVDYAQQIWK
IEEALDETLNEVWDINIDPVSIYNKPHEQLDLIELVKTDHKIFNKVILVFSSICNQTRIL
KETAESKFYSPLTVFGEITGESSEGDVQIEVGKLLPFMIDLSAFVNRCYSLIRNIISQFA
SIYQSQKNIHTQFFKNVHLQAVYYSMIDIFSVLINLDSIITQNTAL---

---NNMYXSELVTELERDIRQLGVSETGLTFLDHFRLLITHIGNAMGYIRLVRSGGLHYC
SNAIKFVPDLKKIPKFQDLTSKDALSPETIQASTNLDSIIHNLSNNLSEGTEYFKMLVNV
FATEFRNIANQHLKNFYIIVPALASNYIDHMINSKDKLFKKSKAIGAEALLFTDDGFAIG
LAYILKLLDQNKDFDSLHWFDRIQLKCETDQKRMLAEANAKGIKEDQNHLAIKKIQNFQQ
E*nixils

Frame A:
l**ktnnpi*KMATSYSANNGQNEEYTFDAFEDGSSKLVGEQQLTKLNQFAVDYAQQIWK
IEEALDETLNEVWDINIDPVSIYNKPHEQLDLIELVKTDHKIFNKVILVFSSICNQTRIL
KETAESKFYSPLTVFGEITGESSEGDVQIEVGKLLPFMIDLSAFVNRCYSLIRNIISQFA
SIYQSQKNIHTQFFKNVHLQAVYYSMIDIFSVLINLDSIITQNTAL---

---e*yvpfrvgdrvgkrh*tigclrdwfnlfrsf*tfnhsyw*cygly*igqirwftll
**cyqirtrlekdskiprfnlkrcplt*nnss*yqfgfnhs*sfk*firgy*il*dvgkr
fryrvskyr*stfkeflyhrtclglklhrsydq**r*tl*eiksnrc*sfiih**wlcnw
fglylkiirsk*rfrfsslvrsysiem*nxskenvg*skckryqrgsksfsn*kdskfpt
rieyxnsf

Frame B:
ynkkrttqfrkwphpirlimvkmknihlmhlkmvhlnwlesnn*lnlinlqlimhnkfgr
*kkh*mkh*mkygi*i*iqfqfiinlmnn*i*lnwlkliikfsik*y*yslqfviklef*
kkqqkvnfihh*qylerlqvnhqkvmyklklvnyyhl*liyqhl*idvih*leillvnlh
rsinhkriyihnslrmfiyklsiiq*liyfqy**i*iv*lhkill---

---NNMYXSELVTELERDIRQLGVSETGLTFLDHFRLLITHIGNAMGYIRLVRSGGLHYC
SNAIKFVPDLKKIPKFQDLTSKDALSPETIQASTNLDSIIHNLSNNLSEGTEYFKMLVNV
FATEFRNIANQHLKNFYIIVPALASNYIDHMINSKDKLFKKSKAIGAEALLFTDDGFAIG
LAYILKLLDQNKDFDSLHWFDRIQLKCETDQKRMLAEANAKGIKEDQNHLAIKKIQNFQQ
E*nixils

Frame C:
iikneqpnlenghilfg**wsk*riyi*ci*rwfi*igwratin*t*sics*lcttnled
rrsir*nik*smgykyrssfnl**ts*tirfn*ig*n*s*nfq*snisilfnl*sn*nfk
rnsrk*ilftinsiwrdyr*iirr*ctn*sw*iitiyd*fisick*mlfin*kyy*sici
dlsitkeytytil*ecsftscllfnd*yifsinkfr*ynytkycf---

---iictxqsw*qswketldnwvsprlv*pf*iildf*slilvmlwvildwsdpvvytiv
vmlsnsyqt*krfqnski*pqkmpshlkqfklvpiwiqsfiifqiiyqrvlntlrcw*tf
slqsfeislini*risissylpwpqitsii*sivkinslrnqkqsvlklyyslmmalqlv
wlis*ny*ikikisilfigsivfn*nvkxikrecwlkqmqkvskrikii*qlkrfkisnk
nrixkffl

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHE452 (SHE452Q) /CSM/SH/SHE4-C/SHE452Q.Seq.d/                       2748   0.0
SHB243 (SHB243Q) /CSM/SH/SHB2-B/SHB243Q.Seq.d/                       1443   0.0
VHP871 (VHP871Q) /CSM/VH/VHP8-C/VHP871Q.Seq.d/                       1417   0.0
VHK424 (VHK424Q) /CSM/VH/VHK4-A/VHK424Q.Seq.d/                       1417   0.0
VHB821 (VHB821Q) /CSM/VH/VHB8-A/VHB821Q.Seq.d/                       1417   0.0
VHB614 (VHB614Q) /CSM/VH/VHB6-A/VHB614Q.Seq.d/                       1417   0.0
VHA684 (VHA684Q) /CSM/VH/VHA6-D/VHA684Q.Seq.d/                       1417   0.0
SHD280 (SHD280Q) /CSM/SH/SHD2-D/SHD280Q.Seq.d/                       1417   0.0
CHL807 (CHL807Q) /CSM/CH/CHL8-A/CHL807Q.Seq.d/                       1417   0.0
CHG805 (CHG805Q) /CSM/CH/CHG8-A/CHG805Q.Seq.d/                       1417   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BU746157|BU746157.1 CH3#002_C07T7 Canine heart normalized cDNA Library in pBluescriptCanis familiaris cDNA clone CH3#002_C07 5', mRNA sequence.    38   8e-04  3
BY464171|BY464171.1 Mus musculus 15 days pregnant adult female amnion cDNA, RIKEN full-length enriched library, clone:K630150M24, 3' end partial sequence.    42   0.028  2
AK113047|AK113047.1 Ciona intestinalis cDNA, clone:ciad002a21, full insert sequence.    48   0.034  2
AC168241|AC168241.2 Bos taurus clone CH240-197K22, *** SEQUENCING IN PROGRESS ***, 28 unordered pieces.    52   0.035  1
CR450713|CR450713.2 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    38   0.12   8
BB822673|BB822673.1 Mus musculus mammary gland RCB-0526 Jyg-MC(A) cDNA, RIKEN full-length enriched library, clone:G830022O04, 3' end partial sequence.    32   0.12   4
CR044130|CR044130.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP186g09.    50   0.14   1
CR023465|CR023465.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP421e10.    50   0.14   1
CR017539|CR017539.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP47e19.    50   0.14   1
CR034434|CR034434.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP47e19.    50   0.14   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54R74) RecName: Full=UPF0681 protein;                               453   e-126
BC040936_1(BC040936|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   230   8e-59
AB028956_1(AB028956|pid:none) Homo sapiens mRNA for KIAA1033 pro...   230   1e-58
BC143373_1(BC143373|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   230   1e-58
BC020314_1(BC020314|pid:none) Mus musculus RIKEN cDNA A230046K03...   227   7e-58
(Q3UMB9) RecName: Full=UPF0681 protein KIAA1033;                      227   7e-58
AK161247_1(AK161247|pid:none) Mus musculus adult male testis cDN...   227   7e-58
AK145000_1(AK145000|pid:none) Mus musculus mammary gland RCB-052...   227   7e-58
BC110850_1(BC110850|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   176   2e-42
CP000600_221(CP000600|pid:none) Ostreococcus lucimarinus CCE9901...   172   3e-41

 psg: 0.79  gvh: 0.25  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 40.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for SHE452 is nuc

>SHE452F.Seq
CTATAATAAAAAACGAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAAT
GGTCAAAATGAAGAATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGA
GAGCAACAATTAACTAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAG
ATAGAAGAAGCATTAGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTT
TCAATTTATAATAAACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCAT
AAAATTTTCAATAAAGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTA
AAAGAAACAGCAGAAAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGT
GAATCATCAGAAGGTGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGAT
TTATCAGCATTTGTAAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCA
TCGATCTATCAATCACAAAAGAATATACATACACAATTCTTTAAGAATGTTCATTTACAA
GCTGTCTATTATTCAATGATTGATATATTTTCAGTATTAATAAATTTAGATAGTATAATT
ACACAAAATACTGCTTTANNNNNNNNNN

>SHE452Z.Seq
NNNNNNNNNNGAATAATATGTACCNTTCAGAGTTGGTGACAGAGTTGGAAAGAGACATTA
GACAATTGGGTGTCTCCGAGACTGGTTTAACCTTTTTAGATCATTTTAGACTTTTAATCA
CTCATATTGGTAATGCTATGGGTTATATTAGATTGGTCAGATCCGGTGGTTTACACTATT
GTAGTAATGCTATCAAATTCGTACCAGACTTGAAAAAGATTCCAAAATTCCAAGATTTAA
CCTCAAAAGATGCCCTCTCACCTGAAACAATTCAAGCTAGTACCAATTTGGATTCAATCA
TTCATAATCTTTCAAATAATTTATCAGAGGGTACTGAATACTTTAAGATGTTGGTAAACG
TTTTCGCTACAGAGTTTCGAAATATCGCTAATCAACATTTAAAGAATTTCTATATCATCG
TACCTGCCTTGGCCTCAAATTACATCGATCATATGATCAATAGTAAAGATAAACTCTTTA
AGAAATCAAAAGCAATCGGTGCTGAAGCTTTATTATTCACTGATGATGGCTTTGCAATTG
GTTTGGCTTATATCTTAAAATTATTAGATCAAAATAAAGATTTCGATTCTCTTCATTGGT
TCGATCGTATTCAATTGAAATGTGAAACNGATCAAAAGAGAATGTTGGCTGAAGCAAATG
CAAAAGGTATCAAAGAGGATCAAAATCATTTAGCAATTAAAAAGATTCAAAATTTCCAAC
AAGAATAGAATATTNAAATTCTTTCTC

>SHE452P.Seq
CTATAATAAAAAACGAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAAT
GGTCAAAATGAAGAATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGA
GAGCAACAATTAACTAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAG
ATAGAAGAAGCATTAGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTT
TCAATTTATAATAAACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCAT
AAAATTTTCAATAAAGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTA
AAAGAAACAGCAGAAAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGT
GAATCATCAGAAGGTGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGAT
TTATCAGCATTTGTAAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCA
TCGATCTATCAATCACAAAAGAATATACATACACAATTCTTTAAGAATGTTCATTTACAA
GCTGTCTATTATTCAATGATTGATATATTTTCAGTATTAATAAATTTAGATAGTATAATT
ACACAAAATACTGCTTTA----------GAATAATATGTACCNTTCAGAGTTGGTGACAG
AGTTGGAAAGAGACATTAGACAATTGGGTGTCTCCGAGACTGGTTTAACCTTTTTAGATC
ATTTTAGACTTTTAATCACTCATATTGGTAATGCTATGGGTTATATTAGATTGGTCAGAT
CCGGTGGTTTACACTATTGTAGTAATGCTATCAAATTCGTACCAGACTTGAAAAAGATTC
CAAAATTCCAAGATTTAACCTCAAAAGATGCCCTCTCACCTGAAACAATTCAAGCTAGTA
CCAATTTGGATTCAATCATTCATAATCTTTCAAATAATTTATCAGAGGGTACTGAATACT
TTAAGATGTTGGTAAACGTTTTCGCTACAGAGTTTCGAAATATCGCTAATCAACATTTAA
AGAATTTCTATATCATCGTACCTGCCTTGGCCTCAAATTACATCGATCATATGATCAATA
GTAAAGATAAACTCTTTAAGAAATCAAAAGCAATCGGTGCTGAAGCTTTATTATTCACTG
ATGATGGCTTTGCAATTGGTTTGGCTTATATCTTAAAATTATTAGATCAAAATAAAGATT
TCGATTCTCTTCATTGGTTCGATCGTATTCAATTGAAATGTGAAACNGATCAAAAGAGAA
TGTTGGCTGAAGCAAATGCAAAAGGTATCAAAGAGGATCAAAATCATTTAGCAATTAAAA
AGATTCAAAATTTCCAACAAGAATAGAATATTNAAATTCTTTCTC