>SHE365 (SHE365Q) /CSM/SH/SHE3-C/SHE365Q.Seq.d/
ATGAGCCTCGTCAAGCGGCAATTAGTGGTCTTTGTATTTTATATTCCAACGAGAATTACA
TCAATCAATTGGATCCGTTCACTCAATGTTTTAAACATAGAATTGTGGAGATTGCATTCT
CTGATAAAACTCCATCCATTGCTGTTGAGGCAATTCGTTTGGTTAGTATAATGTCTACTT
TGGATCTATTGGATGAATCGGATGTTCAAAAGATATGTACACTCTATCTAGTTGATCAAC
CAGAGATATCCAAAGCAGCTGGTGGTTTAATATTTTCAAAGTATCTTTTATCCACTCAAA
CTAAAATCGATACAACTTTGTCAAATATTATAAATTCAACTTCAGCCGCTTCCACCACGT
CAGGTCAGCATGCTAGAAAGAAATCAAAAACTACTAATCTCACATCTGCAGATTTAGATG
AACAACAACAACAACAAATTAGCTCACAAATTTATCATCATCGTGAAACTCAATTGAATG
CACTATTGGAATTTTTAGATAAAACAGTTATACCTGATGAACCTTATTATTTGGTTCAAT
CACTTTGGTCAACCAATGGTAAATCATTCTTTATTGATTXXXXXXXXXXCCAATTCAGTC
TGGTAAATTTGAAATGGAAGTTGTCATTGATCTTATCAATTCATTCCAATCAACTTGCCT
TAAAGAGGATGTCGCTTCAAATGCCGATTTCCAACATCATATGTTTTCAAGATTCCGTTC
CCTTTGTCAATGGTTAGTAACTGACTTTATTAAACATCCTGATACTCTCAGAGGTGTAAT
TGAACACTTTTTAAATTATGCTTTAAAGAATCCACAAGCTGAAAAATTAGAAGAATTATT
AGAAATCTTGGATTTCTTAGGTTCAAAACTTTCTGAAAAATCTTCAATTGAATTTATTAA
AAAAAGAATAATATTTAGTGATGACTTCCAACATATTGATTTAATTAATAAAATTAATAA
TACTATTGAATTTCAAACTATTCAAAATCTTGAACCTTCTGGAAAAACTTCAGGTGGTAA
GAAAATAAAACAACAATTAAAATTTGTTTCTCCAACTAAAAAAACTACACCAAAGAAAAG
AAGAAGAAAATCTTCAAAAGAAAGTGAAAGTGAAGAGAGTGAACCTGATAGTGAAGAAAG
TGATTATGAAGAAGATAGAAGAGAAGAATTACAAAGACAAAAGAAAATCATAAAAGATCA
AATTCAAAAAGATCAAGATCAAGAAATNAAGAACAAAGAGCATCATCGAAGATTCTTCGA
TTTNAAGTTCCAACGATCCAAAAAAGAAATNAAAA

EPRQAAISGLCILYSNENYINQLDPFTQCFKHRIVEIAFSDKTPSIAVEAIRLVSIMSTL
DLLDESDVQKICTLYLVDQPEISKAAGGLIFSKYLLSTQTKIDTTLSNIINSTSAASTTS
GQHARKKSKTTNLTSADLDEQQQQQISSQIYHHRETQLNALLEFLDKTVIPDEPYYLVQS
LWSTNGKSFFID---

---PIQSGKFEMEVVIDLINSFQSTCLKEDVASNADFQHHMFSRFRSLCQWLVTDFIKHP
DTLRGVIEHFLNYALKNPQAEKLEELLEILDFLGSKLSEKSSIEFIKKRIIFSDDFQHID
LINKINNTIEFQTIQNLEPSGKTSGGKKIKQQLKFVSPTKKTTPKKRRRKSSKESESEES
EPDSEESDYEEDRREELQRQKKIIKDQIQKDQDQEXKNKEHHRRFFDXKFQRSKKEXK

Frame A:
mslvkrqlvvfvfyiptritsinwirslnvlnielwrlhsliklhplllrqfvwlv*cll
wiywmnrmfkryvhsi*linqrypkqlvv*yfqsifyplklksiqlcqil*iqlqplppr
qvsmlernqkllishlqi*mnnnnnklahkfiiivkln*mhywnf*ikqlylmnliiwfn
hfgqpmvnhslli---

---PIQSGKFEMEVVIDLINSFQSTCLKEDVASNADFQHHMFSRFRSLCQWLVTDFIKHP
DTLRGVIEHFLNYALKNPQAEKLEELLEILDFLGSKLSEKSSIEFIKKRIIFSDDFQHID
LINKINNTIEFQTIQNLEPSGKTSGGKKIKQQLKFVSPTKKTTPKKRRRKSSKESESEES
EPDSEESDYEEDRREELQRQKKIIKDQIQKDQDQEXKNKEHHRRFFDXKFQRSKKEXK

Frame B:
*asssgn*wslyfifqrelhqsigsvhsmf*t*ncgdcil**nsihcc*gnsfg*ynvyf
gsig*igcskdmytlss*strdiqsswwfnifkvsfihsn*nrynfvkyykfnfsrfhhv
rsac*keikny*shicrfr*tttttn*ltnlsss*nsiectigifr*nsyt**tllfgsi
tlvnqw*iily*---

---qfslvnlkwklslilsihsnqlalkrmslqmpisniicfqdsvpfvng**ltllnil
ilsev*lntf*iml*rihklkn*kny*kswis*vqnflknlqlnllkke*ylvmtsnili
*likliillnfklfkilnlleklqvvrk*nnn*nlflqlkklhqrkeeenlqkkvkvkrv
nlivkkvimkkieeknykdkrks*kikfkkikikkxrtksiiedssixssndpkkkxk

Frame C:
EPRQAAISGLCILYSNENYINQLDPFTQCFKHRIVEIAFSDKTPSIAVEAIRLVSIMSTL
DLLDESDVQKICTLYLVDQPEISKAAGGLIFSKYLLSTQTKIDTTLSNIINSTSAASTTS
GQHARKKSKTTNLTSADLDEQQQQQISSQIYHHRETQLNALLEFLDKTVIPDEPYYLVQS
LWSTNGKSFFID---

---nsvw*i*ngsch*syqfipinlp*rgcrfkcrfptsyvfkipfplsmvsn*ly*ts*
ysqrcn*tlfklcfkests*kirriirnlgflrfktf*kifn*iy*kknni***lpty*f
n**n**yy*isnysks*tfwknfrw*enkttikicfsn*knytkekkkkifkrk*k*re*
t***rk*l*rr*krritktkenhkrsnskrsrsrnxeqrasskilrfxvptiqkrnx

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHE365 (SHE365Q) /CSM/SH/SHE3-C/SHE365Q.Seq.d/                       1128   0.0
AHL488 (AHL488Q) /CSM/AH/AHL4-D/AHL488Q.Seq.d/                        886   0.0
CHJ363 (CHJ363Q) /CSM/CH/CHJ3-C/CHJ363Q.Seq.d/                        733   0.0
AHQ566 (AHQ566Q) /CSM/AH/AHQ5-C/AHQ566Q.Seq.d/                        620   e-176
AHE482 (AHE482Q) /CSM/AH/AHE4-D/AHE482Q.Seq.d/                        549   e-155
VHK738 (VHK738Q) /CSM/VH/VHK7-B/VHK738Q.Seq.d/                         40   0.083
VHJ615 (VHJ615Q) /CSM/VH/VHJ6-A/VHJ615Q.Seq.d/                         40   0.083
CHP504 (CHP504Q) /CSM/CH/CHP5-A/CHP504Q.Seq.d/                         40   0.083
CFK339 (CFK339Q) /CSM/CF/CFK3-B/CFK339Q.Seq.d/                         40   0.083
AHR293 (AHR293Q) /CSM/AH/AHR2-D/AHR293Q.Seq.d/                         40   0.083

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    32   0.097  13
AL953844|AL953844.7 Zebrafish DNA sequence from clone DKEY-29G23 in linkage group 15.    50   0.12   1
BX842643|BX842643.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 2/4.    46   0.32   12
AC179101|AC179101.1 Strongylocentrotus purpuratus clone R3-4011C24, WORKING DRAFT SEQUENCE, 12 unordered pieces.    36   0.33   7
CL889644|CL889644.1 abf96d04.x1 Soybean random, unfiltered genomic library Glycine max genomic, genomic survey sequence.    40   0.34   2
AQ155894|AQ155894.1 HS_3197_B1_A02_T7 CIT Approved Human Genomic Sperm Library D Homo sapiens genomic clone Plate=3197 Col=3 Row=B, DNA sequence.    44   0.37   2
AC110435|AC110435.5 Rattus norvegicus clone CH230-288I22, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    48   0.49   1
AC163156|AC163156.2 Bos taurus clone CH240-78K11, *** SEQUENCING IN PROGRESS ***, 59 unordered pieces.    48   0.49   1
AF459639|AF459639.1 Triticum monococcum BAC clones 116F2 and 115G1 gene sequence.    48   0.49   1
AB182464|AB182464.1 Pichia membranifaciens mitochondrial gene for small subunit ribosomal RNA, partial sequence.    48   0.49   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM920436_198(AM920436|pid:none) Penicillium chrysogenum Wisconsi...    50   1e-04
BC161103_1(BC161103|pid:none) Xenopus tropicalis hypothetical pr...    49   4e-04
CR786576_4(CR786576|pid:none) Zebrafish DNA sequence from clone ...    45   0.004
BC098993_1(BC098993|pid:none) Xenopus laevis hypothetical protei...    45   0.005
(Q8WVM7) RecName: Full=Cohesin subunit SA-1; AltName: Full=Strom...    45   0.007
BC062954_1(BC062954|pid:none) Mus musculus stromal antigen 1, mR...    45   0.007
AK048671_1(AK048671|pid:none) Mus musculus 0 day neonate cerebel...    45   0.007
(Q9DGN1) RecName: Full=Cohesin subunit SA-1;          Short=xSA-...    45   0.007
CS300513_1(CS300513|pid:none) Sequence 21 from Patent EP1661986.       42   0.035
AB179263_1(AB179263|pid:none) Macaca fascicularis testis cDNA cl...    42   0.045

 psg: 0.93  gvh: 0.32  alm: 0.37  top: 0.40  tms: 0.00  mit: 0.21  mip: 0.02
 nuc: 0.21  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: nuclear
	 32.0 %: cytoplasmic
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for SHE365 is nuc

>SHE365F.Seq
ATGAGCCTCGTCAAGCGGCAATTAGTGGTCTTTGTATTTTATATTCCAACGAGAATTACA
TCAATCAATTGGATCCGTTCACTCAATGTTTTAAACATAGAATTGTGGAGATTGCATTCT
CTGATAAAACTCCATCCATTGCTGTTGAGGCAATTCGTTTGGTTAGTATAATGTCTACTT
TGGATCTATTGGATGAATCGGATGTTCAAAAGATATGTACACTCTATCTAGTTGATCAAC
CAGAGATATCCAAAGCAGCTGGTGGTTTAATATTTTCAAAGTATCTTTTATCCACTCAAA
CTAAAATCGATACAACTTTGTCAAATATTATAAATTCAACTTCAGCCGCTTCCACCACGT
CAGGTCAGCATGCTAGAAAGAAATCAAAAACTACTAATCTCACATCTGCAGATTTAGATG
AACAACAACAACAACAAATTAGCTCACAAATTTATCATCATCGTGAAACTCAATTGAATG
CACTATTGGAATTTTTAGATAAAACAGTTATACCTGATGAACCTTATTATTTGGTTCAAT
CACTTTGGTCAACCAATGGTAAATCATTCTTTATTGATTNNNNNNNNNN

>SHE365Z.Seq
NNNNNNNNNNCCAATTCAGTCTGGTAAATTTGAAATGGAAGTTGTCATTGATCTTATCAA
TTCATTCCAATCAACTTGCCTTAAAGAGGATGTCGCTTCAAATGCCGATTTCCAACATCA
TATGTTTTCAAGATTCCGTTCCCTTTGTCAATGGTTAGTAACTGACTTTATTAAACATCC
TGATACTCTCAGAGGTGTAATTGAACACTTTTTAAATTATGCTTTAAAGAATCCACAAGC
TGAAAAATTAGAAGAATTATTAGAAATCTTGGATTTCTTAGGTTCAAAACTTTCTGAAAA
ATCTTCAATTGAATTTATTAAAAAAAGAATAATATTTAGTGATGACTTCCAACATATTGA
TTTAATTAATAAAATTAATAATACTATTGAATTTCAAACTATTCAAAATCTTGAACCTTC
TGGAAAAACTTCAGGTGGTAAGAAAATAAAACAACAATTAAAATTTGTTTCTCCAACTAA
AAAAACTACACCAAAGAAAAGAAGAAGAAAATCTTCAAAAGAAAGTGAAAGTGAAGAGAG
TGAACCTGATAGTGAAGAAAGTGATTATGAAGAAGATAGAAGAGAAGAATTACAAAGACA
AAAGAAAATCATAAAAGATCAAATTCAAAAAGATCAAGATCAAGAAATNAAGAACAAAGA
GCATCATCGAAGATTCTTCGATTTNAAGTTCCAACGATCCAAAAAAGAAATNAAAA

>SHE365P.Seq
ATGAGCCTCGTCAAGCGGCAATTAGTGGTCTTTGTATTTTATATTCCAACGAGAATTACA
TCAATCAATTGGATCCGTTCACTCAATGTTTTAAACATAGAATTGTGGAGATTGCATTCT
CTGATAAAACTCCATCCATTGCTGTTGAGGCAATTCGTTTGGTTAGTATAATGTCTACTT
TGGATCTATTGGATGAATCGGATGTTCAAAAGATATGTACACTCTATCTAGTTGATCAAC
CAGAGATATCCAAAGCAGCTGGTGGTTTAATATTTTCAAAGTATCTTTTATCCACTCAAA
CTAAAATCGATACAACTTTGTCAAATATTATAAATTCAACTTCAGCCGCTTCCACCACGT
CAGGTCAGCATGCTAGAAAGAAATCAAAAACTACTAATCTCACATCTGCAGATTTAGATG
AACAACAACAACAACAAATTAGCTCACAAATTTATCATCATCGTGAAACTCAATTGAATG
CACTATTGGAATTTTTAGATAAAACAGTTATACCTGATGAACCTTATTATTTGGTTCAAT
CACTTTGGTCAACCAATGGTAAATCATTCTTTATTGATT----------CCAATTCAGTC
TGGTAAATTTGAAATGGAAGTTGTCATTGATCTTATCAATTCATTCCAATCAACTTGCCT
TAAAGAGGATGTCGCTTCAAATGCCGATTTCCAACATCATATGTTTTCAAGATTCCGTTC
CCTTTGTCAATGGTTAGTAACTGACTTTATTAAACATCCTGATACTCTCAGAGGTGTAAT
TGAACACTTTTTAAATTATGCTTTAAAGAATCCACAAGCTGAAAAATTAGAAGAATTATT
AGAAATCTTGGATTTCTTAGGTTCAAAACTTTCTGAAAAATCTTCAATTGAATTTATTAA
AAAAAGAATAATATTTAGTGATGACTTCCAACATATTGATTTAATTAATAAAATTAATAA
TACTATTGAATTTCAAACTATTCAAAATCTTGAACCTTCTGGAAAAACTTCAGGTGGTAA
GAAAATAAAACAACAATTAAAATTTGTTTCTCCAACTAAAAAAACTACACCAAAGAAAAG
AAGAAGAAAATCTTCAAAAGAAAGTGAAAGTGAAGAGAGTGAACCTGATAGTGAAGAAAG
TGATTATGAAGAAGATAGAAGAGAAGAATTACAAAGACAAAAGAAAATCATAAAAGATCA
AATTCAAAAAGATCAAGATCAAGAAATNAAGAACAAAGAGCATCATCGAAGATTCTTCGA
TTTNAAGTTCCAACGATCCAAAAAAGAAATNAAAA